Rorug04G0060200

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011973
Physical Location & Seq
Reverse (-)
9418485 .. 9419265
781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0060200.1

Sequence Viewer

Length: 723 bp
ATGGATGCTCTTAATTGGTTGAATTTGTGTGTTGGGAAGTTATCAAAAACAGACTTTGATCAACTGTTGCATTTTATTTGGGGAATTTGGAAAGAAAGGAATGATAGGGTATGGGAAAACAAAATCACTTTAGCTCGTGATATAGCTCTCATGTCTTCTGTCAGACTGAGTAACTTTATTTTTCACAATACAAAATTACATGGTTCTCAGGGAACTAGGAAAATTTTGAAGTGGCAGTGCCCTCCTATGGGTTGGTTGAAGTCCGTGAATTTTGATGGAGCTTTTAGTCCGAGCTCTGGCAAAGCAGCAATTGGTTTCTTAGTCCGTGACTCTCAAGAGACTTTTTTGGGAGCAGTGGGGAAGGTAGTTACTCGAGTTCAGTCTGCTGAACATGTCGAGCTCTTGGCTTGTAAGGAAGCATTTGGTTATGTGATGGAACATGGTCTATGTCCAGTAATTTTGGAGACTGATTGTTTGGTTTTGAATCAACAAGTGTGTCAGGAGGAGCTGCAAAATTATTCTATTCTTGGAAGGTTATATGAGGACCTCAGACAAGATTTGAACGAGGTGAACCAAGTTAGAATTATTCATGCTAGAAGAGAGGCAAACATGGCTGCGCACACATTGGCAGCTCATGCTGCAGATTTTGATCAAGCTTTCTTTTGGTCTAATATTCCTTCCTTCATTCAAGATGTAATAGAATTTGAGAAATGTATTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

240

Amino Acids

27.37

Weight (kDa)

6.06

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 90 - 213 1.5e-22 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 618
AcsI RAATTY 5 cut(s) 22, 84, 222, 268, 701
AfiI CCNNNNNNNGG 3 cut(s) 247, 248, 296
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 6 cut(s) 22, 229, 259, 484, 562, 689
AluBI AGCT 8 cut(s) 134, 146, 281, 294, 400, 508, 632, 656
AluI AGCT 8 cut(s) 134, 146, 281, 294, 400, 508, 632, 656
Alw21I GWGCWC 2 cut(s) 296, 402
Alw26I GTCTC 2 cut(s) 332, 458
Ama87I CYCGRG 1 cut(s) 372
ApeKI GCWGC 5 cut(s) 305, 508, 614, 629, 638
ApoI RAATTY 5 cut(s) 22, 84, 222, 268, 701
ArsI GACNNNNNNTTYG 2 cut(s) 457, 489
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 1 cut(s) 580
AvaI CYCGRG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 296, 402
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 296, 402
BbvI GCAGC 5 cut(s) 317, 495, 601, 625, 641
BccI CCATC 2 cut(s) 269, 427
BclI TGATCA 2 cut(s) 58, 649
BcoDI GTCTC 2 cut(s) 332, 458
BfaI CTAG 2 cut(s) 216, 594
BfmI CTRYAG 1 cut(s) 639
BisI GCNGC 5 cut(s) 306, 509, 615, 630, 639
BlsI GCNGC 5 cut(s) 307, 510, 616, 631, 640
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 372
BmgT120I GGNCC 1 cut(s) 544
BpiI GAAGAC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 318
BsaBI GATNNNNATC 1 cut(s) 648
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 248, 296
Bse1I ACTGG 1 cut(s) 452
Bse8I GATNNNNATC 1 cut(s) 648
BseGI GGATG 1 cut(s) 10
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 3 cut(s) 247, 248, 296
BseMII CTCAG 3 cut(s) 158, 221, 562
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 5 cut(s) 317, 495, 601, 625, 641
BsiHKAI GWGCWC 2 cut(s) 296, 402
BsiHKCI CYCGRG 1 cut(s) 372
BslI CCNNNNNNNGG 3 cut(s) 247, 248, 296
BsmAI GTCTC 2 cut(s) 332, 458
BsoBI CYCGRG 1 cut(s) 372
Bsp1286I GDGCHC 3 cut(s) 242, 296, 402
Bsp143I GATC 2 cut(s) 58, 649
BspCNI CTCAG 3 cut(s) 159, 220, 561
BspMAI CTGCAG 1 cut(s) 643
BsrI ACTGG 1 cut(s) 452
BssMI GATC 2 cut(s) 58, 649
BssSI CACGAG 1 cut(s) 135
Bst2BI CACGAG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 66
Bst6I CTCTTC 1 cut(s) 592
BstAPI GCANNNNNTGC 1 cut(s) 635
BstDEI CTNAG 4 cut(s) 167, 207, 319, 548
BstF5I GGATG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 2 cut(s) 61, 652
BstMAI GTCTC 2 cut(s) 332, 458
BstMBI GATC 2 cut(s) 58, 649
BstMWI GCNNNNNNNGC 3 cut(s) 611, 635, 638
BstNSI RCATGY 1 cut(s) 395
BstSFI CTRYAG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 242
BstV1I GCAGC 5 cut(s) 317, 495, 601, 625, 641
BstV2I GAAGAC 1 cut(s) 147
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 2 cut(s) 242, 360
BtsIMutI CAGTG 2 cut(s) 242, 360
CfoI GCGC 1 cut(s) 619
Cfr13I GGNCC 1 cut(s) 544
CviAII CATG 7 cut(s) 151, 200, 392, 440, 590, 610, 635
DdeI CTNAG 4 cut(s) 167, 207, 319, 548
DpnI GATC 2 cut(s) 60, 651
DpnII GATC 2 cut(s) 58, 649
Eam1104I CTCTTC 1 cut(s) 592
EarI CTCTTC 1 cut(s) 592
Ecl136II GAGCTC 2 cut(s) 294, 400
Eco24I GRGCYC 2 cut(s) 296, 402
Eco47I GGWCC 1 cut(s) 544
Eco53kI GAGCTC 2 cut(s) 294, 400
Eco88I CYCGRG 1 cut(s) 372
EcoICRI GAGCTC 2 cut(s) 294, 400
EcoO109I RGGNCCY 1 cut(s) 544
EcoT38I GRGCYC 2 cut(s) 296, 402
FaeI CATG 7 cut(s) 154, 203, 395, 443, 593, 613, 638
FatI CATG 7 cut(s) 150, 199, 391, 439, 589, 609, 634
FbaI TGATCA 2 cut(s) 58, 649
Fnu4HI GCNGC 5 cut(s) 306, 509, 615, 630, 639
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 2 cut(s) 296, 402
Fsp4HI GCNGC 5 cut(s) 306, 509, 615, 630, 639
FspBI CTAG 2 cut(s) 216, 594
FspI TGCGCA 1 cut(s) 618
GlaI GCGC 1 cut(s) 618
GluI GCNGC 5 cut(s) 306, 509, 615, 630, 639
HhaI GCGC 1 cut(s) 619
Hin1II CATG 7 cut(s) 154, 203, 395, 443, 593, 613, 638
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 2 cut(s) 329, 484
HphI GGTGA 1 cut(s) 580
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 3 cut(s) 164, 291, 551
Hpy188III TCNNGA 4 cut(s) 137, 335, 500, 689
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 4 cut(s) 355, 525, 687, 691
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4V TGCA 3 cut(s) 70, 511, 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 611, 635, 638
HpyF3I CTNAG 4 cut(s) 167, 207, 319, 548
Hsp92II CATG 7 cut(s) 154, 203, 395, 443, 593, 613, 638
HspAI GCGC 1 cut(s) 617
Ksp22I TGATCA 2 cut(s) 58, 649
Kzo9I GATC 2 cut(s) 58, 649
LmnI GCTCC 3 cut(s) 278, 350, 505
LpnPI CCDG 4 cut(s) 194, 282, 465, 485
Lsp1109I GCAGC 5 cut(s) 317, 495, 601, 625, 641
MaeI CTAG 2 cut(s) 216, 594
MaeIII GTNAC 3 cut(s) 170, 326, 367
MalI GATC 2 cut(s) 60, 651
MboI GATC 2 cut(s) 58, 649
MboII GAAGA 2 cut(s) 147, 609
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 3 cut(s) 242, 296, 402
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 6 cut(s) 252, 496, 535, 557, 559, 595
MseI TTAA 2 cut(s) 12, 721
MunI CAATTG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 611, 635, 638
NdeII GATC 2 cut(s) 58, 649
NlaIII CATG 7 cut(s) 154, 203, 395, 443, 593, 613, 638
NmuCI GTSAC 1 cut(s) 326
NsbI TGCGCA 1 cut(s) 618
NspI RCATGY 1 cut(s) 395
PaeR7I CTCGAG 1 cut(s) 372
PciI ACATGT 1 cut(s) 391
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 5 cut(s) 307, 510, 616, 631, 640
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 544
PscI ACATGT 1 cut(s) 391
Psp124BI GAGCTC 2 cut(s) 296, 402
Psp5II RGGWCCY 1 cut(s) 544
PspPI GGNCC 1 cut(s) 544
PspPPI RGGWCCY 1 cut(s) 544
PspXI VCTCGAGB 1 cut(s) 372
PstI CTGCAG 1 cut(s) 643
SacI GAGCTC 2 cut(s) 296, 402
SaqAI TTAA 2 cut(s) 12, 721
SatI GCNGC 5 cut(s) 306, 509, 615, 630, 639
Sau3AI GATC 2 cut(s) 58, 649
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 1 cut(s) 323
SduI GDGCHC 3 cut(s) 242, 296, 402
SfcI CTRYAG 1 cut(s) 639
Sfr274I CTCGAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 544
SlaI CTCGAG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 333, 372
SmoI CTYRAG 2 cut(s) 333, 372
SspI AATATT 1 cut(s) 673
SspMI CTAG 2 cut(s) 216, 594
SstI GAGCTC 2 cut(s) 296, 402
TaaI ACNGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 373, 396
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 2 cut(s) 12, 721
Tru9I TTAA 2 cut(s) 12, 721
TscAI CASTG 2 cut(s) 242, 360
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 5 cut(s) 305, 508, 614, 629, 638
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 2 cut(s) 578, 673
TspGWI ACGGA 2 cut(s) 253, 314
TspRI CASTG 2 cut(s) 242, 360
VpaK11BI GGWCC 1 cut(s) 544
XapI RAATTY 5 cut(s) 22, 84, 222, 268, 701
XceI RCATGY 1 cut(s) 395
XhoI CTCGAG 1 cut(s) 372
XspI CTAG 2 cut(s) 216, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.