Rh4AG131900

Translation initiation factor eIF3 subunit 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011973
Physical Location & Seq
Reverse (-)
28690183 .. 28709600
19418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG131900.1

Sequence Viewer

Length: 5544 bp
ATGGCTCCCAAAACAGGGAAAGCCAAGCCACACAAATCCAAGGGAGACAAGAAGAAGAAAGAAGAGAAAGTTTTGCCTACCGTAATCGAGATTACCATTGAAACGTCAGATGACTCGCAAGTGACCCTCAAGGGTATATCTACGGACAGGATCTTAGATGTGAGAAAGCTGTTGGGGGTCCACGTAGATACATGTCATTTCACCAATTTCTCCCTATCACACGAGGTGCGAGGACCCAGACTGAAAGATACTGTGGACATCCTCTCTCTCAAACCCTGCCACCTCACCATCGTCGAAGAGGATTACACGGAAGAGCAAGCCGTGGCTCACATTCGAAGACTGGTTGACATCGTCGCCTGCACGACGTCGTTTGGCTCGTCGTCTTCTTCACCCAGAACCCCCGGTTCTGCTTCGGTTCCGGCTCCGGTTCGGTCGAGTTCTAAAGAGTCCGGTTTAGACGAGGGCGACGAGCACAATGCCGTTCCGAAGACCAAAGTTTCGAGTCCGATTCCGATCGCCGGCGAGAAAGGCGGCGAGTCGGCAATGTATCCTCCGCCGAGGCTCGGCCAGTTTTACGACTTCTTCTCCTTGTCTCACCTCACTCCTCCACTCCACTACGTTAGAAGATCTAGCCGTCCGTTCCTTGAGGACAAGGCAGAGGAGGACCTTTTTCAAATTGATGTTCGGGTTTGTAGTGGGAAGCCGACGACTATAGTAGCATCCAGAAAAGGGTTCTACCCTGCTGGAAAACGTCCTCTTATTACTCACTCTCTGGTTGCTTTGCTTCAACAGATAAGCCGGCCATTCGATGCTGCGTACAATGCTGTCATGAAGGCTTTCACCGAGCACAATAAATTTGGGAACCTTCCTTATGGTTTTCGGGCAAACACATGGGTTGTCCCTCCGGTTGGTGCTGAGAACCCGTCTGTTTTCCCGCCACTTCCTCTAGAAGATGAAAATTGGGGAGGAAATGGGGGTGGGCAGGGAAGAGACGGTAAACACGATAACAGGCCATGGGCGAAAGAGTTTGCCATTCTTGCAGCAATGCCTTGTGCAACAGCAGAGGAGAGGCAAGTTCGAGATCGAAAGGCTTTTCTGCTTCACAGTTTATTTGTCGACGTCTCAGTTCTTAAAGCTGTTGCAGCTATTAAGAGCCTAATTGACACTCATCAATCTTGCCTAAATGATTCCACTCTTTCACATCATCATGAGGAAAAAATTGGAGATTTAATCATTAAGATCGTTAGAGATGCACCTGATGCAAGTGTAAAGGTGGATTGCAAGAATGATGGTAGTCGAGTTCTAGGAATGCCACAAGGAGAAGTTACTCAGAGAAACCTACTGAAAGGCATAACAGCTGATGAGAGTGCAACTGTTCATGATACTTCTACACTAGGTGTTGTGGTTGTTAGGCATTGTGGATTTACTGCTGTTGTGAAAGTTTTAGCTGAGGTGAATTGGGTGGGGAAACCTGTTCCTCAGGACATTGAAATTGAGGACCAGCCTGAAGGAGGTGCAAATGCCTTGAATGTTAACAGCCTGAGAATGCTATTGCAACAGTCATCCCTCCTTCAGTCATCAACTACGGTTCAACGAAGCCAGAGTACAGATTTTGAAAATTTACGTTCTGCTAGGTCCTTAGTAAGGAAAGTACTCGAAGAAAGTTTATTAAGGTTGCAGGGTGGGCCATCTAATCACACCAAGTCGATCAGATGGGAGCTAGGAGCATGTTGGGTGCAACATTTGCAAAATCAGGCTTCAGCGAAAAATGAGCCTAAGAAAAATGAAGCTAAGATAGAACCGGCTGTTAAGGGCCTCGGAAAGCAAGGTGGACTGCTAAAGGAAATAAAGAAGAAAATGGATATCAGGAGCAGTAAAACTGAACAGGGCAAGGAAGTTCTTGGGGGTAACAACCTTGATCTTAATAAGAATTCAGTCACCACCAGTCAGGAGGATTTAGAAAAGCGAGATGCCGAAAAGGAAATAAATTGGAGAAGGCTACTTCCTGATGCATCCTATTCGCGGCTCAAAGAATCAGATACCGGTCTCCACCTTAAGTCACCTGATGAGTTGATTGAAATGGCACACAAGTATTACGCTGATACTGCACTTCCAAAACTGGTGGCAGATTTTGGCTCCCTTGAACTTTCACCAGTTGATGGAAGGACATTGACAGATTTTATGCATACAAGGGGTTTGCAAATGTGTTCCCTGGGACGCGTGGTTGAACTTGCAGATAAGCTCCCTCATGTACAATCACTCTGTATCCATGAGATGGTTGTGCGAGCATACAAACACATACTGCAAGCTGTTGTAGCAGCAGTTGATAATGTCGCAGACTTGGCTGCATCCATAGCAGCATGTCTAAATATTCTGCTAGGAACCCCATCACCTGAAAATGTGGATGGAGACATTACTTGTGAGGATACGTTAAAATGGAAGTGGGTGGAAACATTCCTTTTGAAGAGGTTTGGATGGCAATGGAAACATGAAAGCGTTGAGGACTTTAGAAAGTTTGCCATTCTTCGTGGATTGTGCCATAAGGTTGGCCTTGAGCTTGTGCCAAGGGACTATGATATGGACACTTCGTCTCCTTTTAGGAAATCAGATATAGTAAGCATGGTCCCTGTGTACAAGCATGTTGCATGTTCATCTGCTGATGGACGTACACTTTTGGAATCTTCCAAGACTTCCTTGGATAAAGGTAAACTGGAGGATGCTGTTAATTATGGCACTAAGGCGTTGTCAAAACTTGTATCAGTATGTGGGCCTTATCATCGAATGACTGCTGGAGCATATAGTCTTCTCGCTGTAGTACTGTATCACACTGGGGATTTTAATCAGGCAACCATCTATCAGCAAAAAGCATTGGATATTAATGAGAGAGAGCTTGGACTTGATCATCCTGATACAATGAAAAGTTACGGGGATTTAGCTGTGTTCTACTACCGACTTCAACATACAGAGTTGGCCTTGAAGTACGTCAATCGTGCGCTGTATCTTTTGCACCTAACATGTGGGCCGTCTCATCCAAACACGGCTGCCACCTATATCAATGTAGCAATGATGGAAGAAGGTTTGGGGAACGTCCATGTTGCTCTTAGGTACCTCCATGAAGCTCTCAAGTGCAATCAAAGGCTACTTGGAGCTGATCATATACAGACTGCTGCTAGCTATCATGCCATAGCTATCGCACTTTCTTTGATGGAGGCATACTCGTTAAGTGTTCAGCACGAACAAACTACCTTGCAAATTCTGCAGGCGAAACTCGGGTCAGAGGATCTACGCACACAGGATGCGGCTGCATGGCTTGAGTATTTTGAGTCCAAAGCTTTGGAGCAGCAAGAAGCTGCACGGAATGGTACTCCAAAGCCAGATGCTTCAATCTCCAGCAAAGGTCATTTAAGTGTATCAGATCTTTTGGATTATATAACACCAGATGCTGATATGAAAGCTAGAGAGGCCCAAAGGAAAGCTCGTTTGAAGGTTAAAGGCAAACCAGGTCAGAACGGGGAGGCAGTCTCTGATGAGTACCAGAAGGATGAAAATTTGTTACCGAGTCATCCTGTTGCAGAGAATTTAAGTGATAAAGAGAACAAGTCCGAAGCACAATTAGGAGAACCTAGGAATGAGAAATATGATTCCCGTCTACTTGAGCAATCAATAATATTTGCTACACATGATGATCTGGCACAAGATGACAACTCAGATGAAGGCTGGCAAGAGGCTGTTCCTAAAGGCCGGTCACTTATAGGTCGCAAATCTACTGGGTCAAGGAGGCCAAGCCTTGAAAAACTGAATACTAACTTTATCAATACATCCCAGTCATCAAGATATCGAGGCAAGGCCAATAATTTCACATCCCCCAAAACAAGCCCAAATGAGCCTGCTTCTTCAACTGGACCTGCTCCTCCAGTGTCAAAGAAGTTTGTCAAGAGTGCAAGCTTCAGTCCTAAGCAAAACAACTCTAGCACATCAGCTACCGGAGCTGCAGAAAGATTGCCAAACCCAAAGTCAGCACCATCTACCCCAGCTTCTGTTGATCAAGTCATCAAGTCAGTAAGTCCGATCAGTGTTCAGTCAACTGGAAAACTCTTTTCCTACAAAGAAGTTGCATTGGCTCCACCTGGGACAATTGTGAAGGCAGTGGCGGAGCAGTTACCAAAGCCGATTGTACAAATTTCTCAGGTGGGCCAGGAGACACCTACAACAGAGGTCACTGTTGGTGAAGTGACGGCAATTAAAGATGTGAAGGATAAAAAAAAAAAGAAATCGACAGGGGAGAAACAGGTTATGGTTTCTGAGAAAAGCCCTTCTGTAGTACGATCAGCAAAACTGAAGGATTCTGTGGCAAAGGAGTCCTTGGAAGTTGTGAAGCATGCTAGTGTTGGAGTACAAGTTGAAGCTGAATCTGAAGCTGTGGAATTAGAAAACACTGCTTTTGAAGGTGCAGAGGTTGAAAATGTAAAAGTTCCTGTTCCTAGCGTTGAGATTTCTGATGCTTCCCAAAGTCCAAATACCACAGCTGCTGAATGTGGACCATCAGACCCCCTAGGGCCAGACAGTTGCCCCAGGACTTCCTCTGATTCAGAACCTCCATCTGCTTTAACTGAGACTAGAACCAACAATCCTTCTAATACAGGGGAGGGAAAGTCACGTGATTTACCCAATGGCGACATAGTTAAACCAGTGCCAACTGGTGGGGAAAAGGTAGATGAACAAGAAACTGGAAAGGAGACGAGCAAAAAACTTTCTGCAGCCGCACCTCCTTTTAATCCATCCCCCATTCCAGTTTTTGGCTCAATTCCGGTTCCAGTTCCTGGTCTCAAAGATCACGGAGGGATTTTGCCCCCACCTGTAAATATTCCTCCGATGCTTGCAGTTAATCCTGTCCGTAGATCACCCCATCAGTCAGCGACAGCAAGAGTTCCTTATGGTCCACGACTTTCTGGTGGCTATAACAGATCTGGAAGTCGGGTTTCACGTAATAAACATAGCTTTCACAATGGTGAGCACACCGGGGATGGTCCTCCCAGAATAATGAACCCACATGCAGCTGAGTTTGTACCTGGCCAACCTTGGGTTCAAAATGGCTATCCACTGTCTCCCAATGGTTTCTTGCCTTCCCCAAATGGTTATCCAGTGTCCCCAAATGGATATCCGGTATCACCGAATGGTACTCCGGTGAATCAAAACGGGTCTCCGACATCTCCAGTCAGTTCAGATGAGTCATCACCAGTTGTGACTGCTGATTTTGGTGTTGGAGCAAATACTGAAGGTGCAGGGAAGGAGACTAATGATAAGTTATCAGTACAAGTTGAATGTGACAAGCAACCAATTGAAGGAGAGCTGCAGGAAGAACAGTCTTTAGATAATGTAAACGTTCCTCCTGAAACTGAAGAAAAGCCTATTGATACCGATACAGTTCCTGGTGATACTATTGTGGAAAAGGAGACTAGCAATCTAGTAGTTGAGGAAAAACCAAGCAAATGTTGGGGAGATTATAGCGATAATGAAGCCGAGGTCATTGAGGTTAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0033993 GO:0042221 GO:0050896 GO:0097305 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLU CLU_N TPR_12 eIF3_p135

Protein Analysis

1847

Amino Acids

200.9

Weight (kDa)

6.12

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLU_N PF15044 48 - 118 3.1e-09 Mitochondrial function, CLU-N-term
eIF3_p135 PF12807 716 - 859 6.5e-23 Translation initiation factor eIF3 subunit 135
TPR_12 PF13424 900 - 961 2e-06 Tetratricopeptide repeat
TPR_NPHP3 PF24885 900 - 999 2.8e-07 Nephrocystin-3 TPR domain
TPR_12 PF13424 929 - 999 1.4e-12 Tetratricopeptide repeat
TPR_10 PF13374 929 - 970 2.1e-09 Tetratricopeptide repeat
TPR_12 PF13424 1013 - 1087 2.1e-10 Tetratricopeptide repeat
TPR_10 PF13374 1013 - 1053 4.2e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011973)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28080
fragaria_vesca FvH4_3g24640 FvH4_3g24640 FvH4_3g24640
malus_domestica MD16G1258600.v1.1
prunus_persica Prupe.1G101800_v2.0.a1
pyrus_communis pycom16g22600
rosa_chinensis RchiOBHm_Chr4g0405441
rosa_laevigata RLG00000008842
rosa_multiflora Rmu_co8255623.1_g000001 Rmu_sc0000575.1_g000017 Rmu_sc0000575.1_g000018
rosa_roxburghii Rroxscaffold_5G00349900
rosa_rugosa Rorug04G0060200
rosa_samantha Rh4AG131900 Rh4BG126800 Rh4CG139300 Rh4DG125900
rosa_wichuraiana Rw4G010680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 368, 1122
Acc36I ACCTGC 1 cut(s) 3913
Acc65I GGTACC 1 cut(s) 3105
AccB1I GGYRCC 1 cut(s) 3105
AccB7I CCANNNNNTGG 5 cut(s) 2159, 2275, 4700, 4796, 4820
AccI GTMKAC 2 cut(s) 1116, 3649
AccII CGCG 2 cut(s) 2023, 2220
AciI CCGC 7 cut(s) 531, 554, 935, 2023, 3299, 4151, 4761
AclI AACGTT 1 cut(s) 5382
AclWI GGATC 2 cut(s) 158, 3288
AcoI YGGCCR 3 cut(s) 565, 800, 5071
AcsI RAATTY 7 cut(s) 854, 1618, 1930, 3252, 3547, 3577, 4179
AcuI CTGAAG 8 cut(s) 1527, 1556, 1743, 3931, 4358, 4434, 5295, 5418
AcvI CACGTG 1 cut(s) 4658
AcyI GRCGYC 2 cut(s) 365, 1119
AdeI CACNNNGTG 2 cut(s) 226, 1397
AflII CTTAAG 1 cut(s) 2054
AflIII ACRYGT 3 cut(s) 191, 2218, 3014
AgeI ACCGGT 1 cut(s) 2042
AhdI GACNNNNNGTC 1 cut(s) 2587
AjnI CCWGG 8 cut(s) 2211, 3499, 4126, 4194, 4571, 4819, 5068, 5428
AjuI GAANNNNNNNTTGG 2 cut(s) 5067, 5099
AleI CACNNNNGTG 1 cut(s) 5007
AloI GAACNNNNNNTCC 2 cut(s) 4612, 4644
Alw21I GWGCWC 3 cut(s) 474, 849, 5016
AlwI GGATC 2 cut(s) 158, 3288
AlwNI CAGNNNCTG 6 cut(s) 3443, 3524, 4037, 4529, 4820, 5429
Ama87I CYCGRG 1 cut(s) 3269
ApoI RAATTY 7 cut(s) 854, 1618, 1930, 3252, 3547, 3577, 4179
ArsI GACNNNNNNTTYG 6 cut(s) 481, 513, 3257, 3289, 3998, 4030
AseI ATTAAT 1 cut(s) 2877
AsiGI ACCGGT 1 cut(s) 2042
Asp700I GAANNNNTTC 1 cut(s) 836
Asp718I GGTACC 1 cut(s) 3105
AspA2I CCTAGG 2 cut(s) 3623, 4552
AspLEI GCGC 1 cut(s) 2995
AsuC2I CCSGG 2 cut(s) 402, 5020
AsuII TTCGAA 1 cut(s) 334
AsuNHI GCTAGC 1 cut(s) 3170
AvaI CYCGRG 1 cut(s) 3269
AvrII CCTAGG 2 cut(s) 3623, 4552
AxyI CCTNAGG 1 cut(s) 1479
BaeI ACNNNNGTAYC 4 cut(s) 5169, 5202, 5427, 5460
BalI TGGCCA 1 cut(s) 5073
BanI GGYRCC 1 cut(s) 3105
BarI GAAGNNNNNNTAC 2 cut(s) 1588, 1620
BauI CACGAG 1 cut(s) 221
BbrPI CACGTG 1 cut(s) 4658
BbsI GAAGAC 4 cut(s) 343, 375, 494, 2795
Bbv12I GWGCWC 3 cut(s) 474, 849, 5016
BbvCI CCTCAGC 1 cut(s) 1449
BceAI ACGGC 6 cut(s) 305, 464, 618, 3007, 3054, 4251
BcgI CGANNNNNNTGC 4 cut(s) 458, 492, 2001, 2035
BciT130I CCWGG 8 cut(s) 2213, 3501, 4128, 4196, 4573, 4821, 5070, 5430
BciVI GTATCC 3 cut(s) 558, 2276, 2419
BclI TGATCA 3 cut(s) 2899, 3151, 4042
BcnI CCSGG 2 cut(s) 402, 5020
BfmI CTRYAG 7 cut(s) 711, 2811, 3257, 3990, 4317, 4755, 5351
BfrI CTTAAG 1 cut(s) 2054
BfuAI ACCTGC 1 cut(s) 3913
BfuI GTATCC 3 cut(s) 558, 2276, 2419
BglII AGATCT 3 cut(s) 626, 3415, 4964
BlnI CCTAGG 2 cut(s) 3623, 4552
BmcAI AGTACT 2 cut(s) 1653, 2817
BmeRI GACNNNNNGTC 1 cut(s) 2587
BmeT110I CYCGRG 1 cut(s) 3269
BmrI ACTGGG 3 cut(s) 2838, 3777, 3817
BmtI GCTAGC 1 cut(s) 3174
BmuI ACTGGG 3 cut(s) 2838, 3777, 3817
BpiI GAAGAC 4 cut(s) 343, 375, 494, 2795
BplI GAGNNNNNCTC 4 cut(s) 3200, 3232, 3506, 3538
BpmI CTGGAG 5 cut(s) 2732, 2811, 3373, 3897, 5196
Bpu10I CCTNAGC 2 cut(s) 1449, 3954
Bpu14I TTCGAA 1 cut(s) 334
BpuEI CTTGAG 6 cut(s) 113, 665, 2573, 3107, 3332, 3674
BpuMI CCSGG 2 cut(s) 402, 5020
BsaAI YACGTR 3 cut(s) 184, 4658, 4985
BsaBI GATNNNNATC 2 cut(s) 512, 2838
BsaHI GRCGYC 2 cut(s) 365, 1119
BsaI GGTCTC 3 cut(s) 2051, 4829, 5205
BsaWI WCCGGW 8 cut(s) 424, 449, 904, 2042, 3983, 4807, 5161, 5182
BsaXI ACNNNNNCTCC 8 cut(s) 569, 599, 2575, 2605, 4383, 4413, 4637, 4667
Bse118I RCCGGY 5 cut(s) 518, 798, 1801, 2042, 3741
Bse21I CCTNAGG 1 cut(s) 1479
Bse3DI GCAATG 4 cut(s) 549, 1050, 2486, 3069
Bse8I GATNNNNATC 2 cut(s) 512, 2838
BseBI CCWGG 8 cut(s) 2213, 3501, 4128, 4196, 4573, 4821, 5070, 5430
BseJI GATNNNNATC 2 cut(s) 512, 2838
BseMI GCAATG 4 cut(s) 549, 1050, 2486, 3069
BseRI GAGGAG 4 cut(s) 594, 674, 1079, 3900
BseYI CCCAGC 1 cut(s) 4030
BsgI GTGCAG 5 cut(s) 343, 2091, 3336, 4470, 5301
Bsh1236I CGCG 2 cut(s) 2023, 2220
Bsh1285I CGRYCG 2 cut(s) 434, 516
BshNI GGYRCC 1 cut(s) 3105
BshTI ACCGGT 1 cut(s) 2042
BsiEI CGRYCG 2 cut(s) 434, 516
BsiHKAI GWGCWC 3 cut(s) 474, 849, 5016
BsiHKCI CYCGRG 1 cut(s) 3269
BslFI GGGAC 6 cut(s) 884, 2229, 2582, 2609, 4144, 5131
BsmBI CGTCTC 5 cut(s) 984, 1126, 2595, 3030, 4730
BsmFI GGGAC 6 cut(s) 884, 2229, 2582, 2609, 4144, 5131
BsmI GAATGC 2 cut(s) 1314, 1551
Bso31I GGTCTC 3 cut(s) 2051, 4829, 5205
BsoBI CYCGRG 1 cut(s) 3269
Bsp119I TTCGAA 1 cut(s) 334
Bsp1286I GDGCHC 3 cut(s) 474, 849, 5016
Bsp1407I TGTACA 3 cut(s) 2251, 2631, 4174
Bsp19I CCATGG 1 cut(s) 1013
BspACI CCGC 7 cut(s) 531, 554, 935, 2023, 3299, 4151, 4761
BspFNI CGCG 2 cut(s) 2023, 2220
BspHI TCATGA 3 cut(s) 828, 1207, 1378
BspMAI CTGCAG 4 cut(s) 3261, 3994, 4759, 5355
BspMI ACCTGC 1 cut(s) 3913
BspOI GCTAGC 1 cut(s) 3174
BspPI GGATC 2 cut(s) 158, 3288
BspQI GCTCTTC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 334
BspT107I GGYRCC 1 cut(s) 3105
BspTI CTTAAG 1 cut(s) 2054
BspTNI GGTCTC 3 cut(s) 2051, 4829, 5205
BsrDI GCAATG 4 cut(s) 549, 1050, 2486, 3069
BsrFI RCCGGY 5 cut(s) 518, 798, 1801, 2042, 3741
BsrGI TGTACA 3 cut(s) 2251, 2631, 4174
BssAI RCCGGY 5 cut(s) 518, 798, 1801, 2042, 3741
BssNI GRCGYC 2 cut(s) 365, 1119
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 8 cut(s) 39, 1013, 2564, 2694, 3623, 4362, 4552, 5078
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 8 cut(s) 2213, 3501, 4128, 4196, 4573, 4821, 5070, 5430
Bst6I CTCTTC 5 cut(s) 57, 291, 306, 982, 2459
BstACI GRCGYC 2 cut(s) 365, 1119
BstAFI CTTAAG 1 cut(s) 2054
BstAPI GCANNNNNTGC 3 cut(s) 1259, 1744, 3256
BstAUI TGTACA 3 cut(s) 2251, 2631, 4174
BstBAI YACGTR 3 cut(s) 184, 4658, 4985
BstBI TTCGAA 1 cut(s) 334
BstDSI CCRYGG 2 cut(s) 321, 1013
BstENI CCTNNNNNAGG 2 cut(s) 1509, 1642
BstFNI CGCG 2 cut(s) 2023, 2220
BstHHI GCGC 1 cut(s) 2995
BstMCI CGRYCG 2 cut(s) 434, 516
BstNI CCWGG 8 cut(s) 2213, 3501, 4128, 4196, 4573, 4821, 5070, 5430
BstNSI RCATGY 8 cut(s) 195, 1731, 2364, 2642, 2649, 3018, 4382, 5054
BstSFI CTRYAG 7 cut(s) 711, 2811, 3257, 3990, 4317, 4755, 5351
BstUI CGCG 2 cut(s) 2023, 2220
BstV2I GAAGAC 4 cut(s) 343, 375, 494, 2795
BstX2I RGATCY 5 cut(s) 150, 626, 3280, 3415, 4964
BstXI CCANNNNNNTGG 2 cut(s) 2546, 3334
BstYI RGATCY 5 cut(s) 150, 626, 3280, 3415, 4964
Bsu36I CCTNAGG 1 cut(s) 1479
BsuI GTATCC 3 cut(s) 558, 2276, 2419
BtgI CCRYGG 2 cut(s) 321, 1013
BtsI GCAGTG 2 cut(s) 4152, 4434
BtsIMutI CAGTG 9 cut(s) 2826, 3921, 4078, 4152, 4218, 4434, 4695, 5099, 5148
BveI ACCTGC 1 cut(s) 3913
CaiI CAGNNNCTG 6 cut(s) 3443, 3524, 4037, 4529, 4820, 5429
CciI TCATGA 3 cut(s) 828, 1207, 1378
CfoI GCGC 1 cut(s) 2995
Cfr10I RCCGGY 5 cut(s) 518, 798, 1801, 2042, 3741
CseI GACGC 1 cut(s) 2226
CsiI ACCWGGT 1 cut(s) 3499
CspAI ACCGGT 1 cut(s) 2042
CspCI CAANNNNNGTGG 2 cut(s) 2103, 2138
DraIII CACNNNGTG 2 cut(s) 226, 1397
DriI GACNNNNNGTC 1 cut(s) 2587
EaeI YGGCCR 3 cut(s) 565, 800, 5071
Eam1104I CTCTTC 5 cut(s) 57, 291, 306, 982, 2459
Eam1105I GACNNNNNGTC 1 cut(s) 2587
EarI CTCTTC 5 cut(s) 57, 291, 306, 982, 2459
EciI GGCGGA 2 cut(s) 543, 4166
Eco130I CCWWGG 8 cut(s) 39, 1013, 2564, 2694, 3623, 4362, 4552, 5078
Eco31I GGTCTC 3 cut(s) 2051, 4829, 5205
Eco32I GATATC 3 cut(s) 1864, 3836, 5159
Eco57I CTGAAG 8 cut(s) 1527, 1556, 1743, 3931, 4358, 4434, 5295, 5418
Eco72I CACGTG 1 cut(s) 4658
Eco81I CCTNAGG 1 cut(s) 1479
Eco88I CYCGRG 1 cut(s) 3269
EcoNI CCTNNNNNAGG 2 cut(s) 1509, 1642
EcoO109I RGGNCCY 4 cut(s) 233, 664, 1635, 1813
EcoRI GAATTC 1 cut(s) 1930
EcoRII CCWGG 8 cut(s) 2211, 3499, 4126, 4194, 4571, 4819, 5068, 5428
EcoRV GATATC 3 cut(s) 1864, 3836, 5159
EcoT14I CCWWGG 8 cut(s) 39, 1013, 2564, 2694, 3623, 4362, 4552, 5078
EcoT22I ATGCAT 2 cut(s) 2014, 2187
ErhI CCWWGG 8 cut(s) 39, 1013, 2564, 2694, 3623, 4362, 4552, 5078
Esp3I CGTCTC 5 cut(s) 984, 1126, 2595, 3030, 4730
FalI AAGNNNNNCTT 6 cut(s) 2678, 2710, 4346, 4378, 4915, 4947
FaqI GGGAC 6 cut(s) 884, 2229, 2582, 2609, 4144, 5131
FauI CCCGC 1 cut(s) 942
FbaI TGATCA 3 cut(s) 2899, 3151, 4042
FblI GTMKAC 2 cut(s) 1116, 3649
GlaI GCGC 1 cut(s) 2994
GsaI CCCAGC 1 cut(s) 4034
GsuI CTGGAG 5 cut(s) 2732, 2811, 3373, 3897, 5196
HgaI GACGC 1 cut(s) 2226
HhaI GCGC 1 cut(s) 2995
Hin1I GRCGYC 2 cut(s) 365, 1119
Hin6I GCGC 1 cut(s) 2993
HinP1I GCGC 1 cut(s) 2993
HincII GTYRAC 4 cut(s) 346, 1117, 1534, 4083
HindII GTYRAC 4 cut(s) 346, 1117, 1534, 4083
HindIII AAGCTT 2 cut(s) 3330, 3943
HpaI GTTAAC 1 cut(s) 1534
Hpy99I CGWCG 8 cut(s) 296, 356, 367, 370, 382, 470, 709, 1121
Hsp92I GRCGYC 2 cut(s) 365, 1119
HspAI GCGC 1 cut(s) 2993
KpnI GGTACC 1 cut(s) 3109
KroI GCCGGC 2 cut(s) 518, 798
KroNI GCCGGC 2 cut(s) 520, 800
Ksp22I TGATCA 3 cut(s) 2899, 3151, 4042
KspAI GTTAAC 1 cut(s) 1534
LguI GCTCTTC 1 cut(s) 306
MabI ACCWGGT 1 cut(s) 3499
MfeI CAATTG 2 cut(s) 4134, 5337
MflI RGATCY 5 cut(s) 150, 626, 3280, 3415, 4964
MhlI GDGCHC 3 cut(s) 474, 849, 5016
MlsI TGGCCA 1 cut(s) 5073
MluI ACGCGT 1 cut(s) 2218
MluNI TGGCCA 1 cut(s) 5073
MlyI GAGTC 8 cut(s) 107, 455, 511, 545, 3332, 3568, 4367, 5237
MmeI TCCRAC 3 cut(s) 4369, 5228, 5242
Mox20I TGGCCA 1 cut(s) 5073
Mph1103I ATGCAT 2 cut(s) 2014, 2187
MreI CGCCGGCG 1 cut(s) 518
MroNI GCCGGC 2 cut(s) 518, 798
MroXI GAANNNNTTC 1 cut(s) 836
MscI TGGCCA 1 cut(s) 5073
MslI CAYNNNNRTG 4 cut(s) 1206, 3405, 4383, 5007
Msp20I TGGCCA 1 cut(s) 5073
MspA1I CMGCKG 3 cut(s) 1358, 4526, 5057
MspCI CTTAAG 1 cut(s) 2054
MunI CAATTG 2 cut(s) 4134, 5337
Mva1269I GAATGC 2 cut(s) 1314, 1551
MvaI CCWGG 8 cut(s) 2213, 3501, 4128, 4196, 4573, 4821, 5070, 5430
MvnI CGCG 2 cut(s) 2023, 2220
NaeI GCCGGC 2 cut(s) 520, 800
NciI CCSGG 2 cut(s) 402, 5020
NcoI CCATGG 1 cut(s) 1013
NgoMIV GCCGGC 2 cut(s) 518, 798
NheI GCTAGC 1 cut(s) 3170
NmeAIII GCCGAG 2 cut(s) 543, 582
NmuCI GTSAC 9 cut(s) 121, 1936, 2058, 3744, 4216, 4231, 4653, 5242, 5324
NsiI ATGCAT 2 cut(s) 2014, 2187
NspI RCATGY 8 cut(s) 195, 1731, 2364, 2642, 2649, 3018, 4382, 5054
NspV TTCGAA 1 cut(s) 334
OliI CACNNNNGTG 1 cut(s) 5007
PaeI GCATGC 1 cut(s) 4382
PagI TCATGA 3 cut(s) 828, 1207, 1378
PasI CCCWGGG 1 cut(s) 2212
PciI ACATGT 2 cut(s) 191, 3014
PciSI GCTCTTC 1 cut(s) 306
PcsI WCGNNNNNNNCGW 4 cut(s) 374, 465, 999, 4981
PctI GAATGC 2 cut(s) 1314, 1551
PdiI GCCGGC 2 cut(s) 520, 800
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 9 cut(s) 508, 1187, 2033, 2678, 3641, 4343, 4409, 4586, 5188
PflFI GACNNNGTC 1 cut(s) 350
PflMI CCANNNNNTGG 5 cut(s) 2159, 2275, 4700, 4796, 4820
PinAI ACCGGT 1 cut(s) 2042
Ple19I CGATCG 1 cut(s) 516
PleI GAGTC 8 cut(s) 107, 454, 510, 544, 3331, 3567, 4366, 5236
PmaCI CACGTG 1 cut(s) 4658
PmlI CACGTG 1 cut(s) 4658
PpsI GAGTC 8 cut(s) 107, 454, 510, 544, 3331, 3567, 4366, 5236
Ppu21I YACGTR 3 cut(s) 184, 4658, 4985
PpuMI RGGWCCY 3 cut(s) 233, 664, 1635
PscI ACATGT 2 cut(s) 191, 3014
PshBI ATTAAT 1 cut(s) 2877
Psp1406I AACGTT 1 cut(s) 5382
Psp5II RGGWCCY 3 cut(s) 233, 664, 1635
Psp6I CCWGG 8 cut(s) 2211, 3499, 4126, 4194, 4571, 4819, 5068, 5428
PspCI CACGTG 1 cut(s) 4658
PspFI CCCAGC 1 cut(s) 4030
PspGI CCWGG 8 cut(s) 2211, 3499, 4126, 4194, 4571, 4819, 5068, 5428
PspPPI RGGWCCY 3 cut(s) 233, 664, 1635
PstI CTGCAG 4 cut(s) 3261, 3994, 4759, 5355
PstNI CAGNNNCTG 6 cut(s) 3443, 3524, 4037, 4529, 4820, 5429
PsuI RGATCY 5 cut(s) 150, 626, 3280, 3415, 4964
PsyI GACNNNGTC 1 cut(s) 350
PvuI CGATCG 1 cut(s) 516
PvuII CAGCTG 3 cut(s) 1358, 4526, 5057
RseI CAYNNNNRTG 4 cut(s) 1206, 3405, 4383, 5007
SalI GTCGAC 1 cut(s) 1115
SapI GCTCTTC 1 cut(s) 306
ScaI AGTACT 2 cut(s) 1653, 2817
SchI GAGTC 8 cut(s) 107, 455, 511, 545, 3332, 3568, 4367, 5237
SduI GDGCHC 3 cut(s) 474, 849, 5016
SexAI ACCWGGT 1 cut(s) 3499
SfcI CTRYAG 7 cut(s) 711, 2811, 3257, 3990, 4317, 4755, 5351
SfuI TTCGAA 1 cut(s) 334
SgrAI CRCCGGYG 1 cut(s) 518
SmiMI CAYNNNNRTG 4 cut(s) 1206, 3405, 4383, 5007
SmlI CTYRAG 7 cut(s) 128, 644, 2054, 2552, 3122, 3311, 3653
SmoI CTYRAG 7 cut(s) 128, 644, 2054, 2552, 3122, 3311, 3653
SphI GCATGC 1 cut(s) 4382
SsiI CCGC 7 cut(s) 531, 554, 935, 2023, 3299, 4151, 4761
SspI AATATT 3 cut(s) 2371, 3669, 4864
StyI CCWWGG 8 cut(s) 39, 1013, 2564, 2694, 3623, 4362, 4552, 5078
TaqII GACCGA 1 cut(s) 420
TatI WGTACW 8 cut(s) 1604, 1651, 2251, 2631, 2815, 4174, 4393, 5311
TauI GCSGC 4 cut(s) 534, 2026, 3302, 4763
TfiI GAWTC 9 cut(s) 508, 1187, 2033, 2678, 3641, 4343, 4409, 4586, 5188
TscAI CASTG 9 cut(s) 2833, 3921, 4078, 4152, 4225, 4441, 4695, 5106, 5148
TseFI GTSAC 9 cut(s) 121, 1936, 2058, 3744, 4216, 4231, 4653, 5242, 5324
Tsp45I GTSAC 9 cut(s) 121, 1936, 2058, 3744, 4216, 4231, 4653, 5242, 5324
TspGWI ACGGA 6 cut(s) 158, 323, 627, 3370, 4851, 4883
TspRI CASTG 9 cut(s) 2833, 3921, 4078, 4152, 4225, 4441, 4695, 5106, 5148
Tth111I GACNNNGTC 1 cut(s) 350
Van91I CCANNNNNTGG 5 cut(s) 2159, 2275, 4700, 4796, 4820
Vha464I CTTAAG 1 cut(s) 2054
VspI ATTAAT 1 cut(s) 2877
XagI CCTNNNNNAGG 2 cut(s) 1509, 1642
XapI RAATTY 7 cut(s) 854, 1618, 1930, 3252, 3547, 3577, 4179
XbaI TCTAGA 1 cut(s) 946
XceI RCATGY 8 cut(s) 195, 1731, 2364, 2642, 2649, 3018, 4382, 5054
XcmI CCANNNNNNNNNTGG 2 cut(s) 2692, 5490
XmaJI CCTAGG 2 cut(s) 3623, 4552
XmiI GTMKAC 2 cut(s) 1116, 3649
XmnI GAANNNNTTC 1 cut(s) 836
ZraI GACGTC 2 cut(s) 366, 1120
ZrmI AGTACT 2 cut(s) 1653, 2817
Zsp2I ATGCAT 2 cut(s) 2014, 2187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.