AT4G28300

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010117
Physical Location & Seq
Forward (+)
14014646 .. 14017097
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28300.1

Sequence Viewer

Length: 1491 bp
ATGGCGTCTGGATCTTCGGGTCGGGTCAACTCAGGATCCAAAGGTTTCGATTTTGGCTCCGATGATATCCTTTGCTCTTATGATGACTACACCAACCAAGACTCCTCTAATGGTCCTCACTCCGATCCTGCGATCGCTGCTTCCAACTCCAACAAGGAATTCCATAAAACAAGGATGGCAAGGTCTTCGGTTTTCCCTACAAGCTCTTACAGTCCACCAGAGGATTCTTTGAGCCAAGACATTACTGACACTGTCGAAAGGACCATGAAGATGTATGCTGATAACATGATGCGCTTTCTTGAAGGCCTCAGTTCACGCTTGTCTCAGCTTGAACTCTACTGCTACAACCTTGATAAGACCATTGGAGAAATGAGATCGGAGTTGACTCATGCACATGAGGATGCCGATGTGAAGCTTAGATCTCTCGACAAACATCTTCAAGAGGTGCATAGGTCTGTCCAGATTCTTAGAGACAAACAAGAGCTAGCAGATACTCAGAAAGAGCTAGCGAAGCTACAACTTGTGCAGAAAGAGTCATCTTCGTCGTCTCACTCTCAACATGGTGAGGACCGTGTCGCCACTCCTGTTCCAGAGCCTAAGAAGAGCGAGAACACCTCTGATGCACACAACCAGCAGCTTGCACTTGCTTTGCCTCACCAAATAGCCCCACAGCCACAGGTGCAGCCGCAGCCACAGCCACAACAACATCAGTATTACATGCCTCCTCCTCCTACACAGCTTCAAAACACACCTGCACCGGTGCCCGTTTCTACTCCACCATCACAACTTCAAGCACCACCAGCGCAGAGTCAGTTCATGCCCCCACCTCCTGCTCCATCTCACCCAAGCTCAGCCCAAACTCAGTCATTCCCGCAGTACCAGCAAAACTGGCCACCCCAACCACAAGCCAGGCCACAGTCAAGTGGAGGCTACCCAACATACTCGCCTGCACCACCAGGCAACCAACCGCCAGTAGAATCGTTGCCAAGCAGCATGCAGATGCAATCACCATACTCTGGACCTCCTCAACAGTCTATGCAAGCTTACGGATACGGTGCTGCACCACCACCACAGGCTCCTCCTCAGCAGACAAAGATGTCTTACAGCCCCCAGACTGGCGATGGGTATCTACCCTCAGGACCTCCTCCTCCTTCCGGGTATGCCAATGCCATGTATGAAGGTGGGCGAATGCAATACCCACCACCTCAGCCGCAGCAGCAGCAGCAGCAAGCCCATTACTTGCAAGGTCCACAAGGTGGTGGATACTCTCCCCAGCCGCACCAGGCAGGTGGCGGTAACATTGGTGCACCACCTGTGTTGAGATCAAAATACGGTGAACTGATAGAGAAGCTAGTGAGCATGGGTTTCAGAGGAGACCATGTCATGGCTGTTATTCAGCGGATGGAAGAGAGTGGCCAACCCATCGACTTCAACACCCTCCTCGACAGATTGAGTGGGCAGTCCTCAGGAGGACCTCCCAGAGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

496

Amino Acids

54.11

Weight (kDa)

5.82

Isoelectric Point (pI)

83.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 444 - 487 1.5e-23 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 760, 1277
AasI GACNNNNNNGTC 1 cut(s) 1096
Acc36I ACCTGC 2 cut(s) 760, 1277
AccB1I GGYRCC 1 cut(s) 760
AccB7I CCANNNNNTGG 4 cut(s) 1016, 1256, 1384, 1485
AciI CCGC 7 cut(s) 686, 872, 968, 1211, 1277, 1293, 1399
AclWI GGATC 4 cut(s) 19, 30, 43, 119
AcoI YGGCCR 2 cut(s) 890, 1414
AcsI RAATTY 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1256
AfaI GTAC 1 cut(s) 878
AfiI CCNNNNNNNGG 7 cut(s) 1016, 1115, 1154, 1256, 1384, 1481, 1485
AgeI ACCGGT 1 cut(s) 757
AgsI TTSAA 6 cut(s) 302, 332, 440, 743, 791, 1432
AjnI CCWGG 3 cut(s) 908, 955, 1281
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
Alw21I GWGCWC 1 cut(s) 1309
Alw26I GTCTC 4 cut(s) 327, 465, 552, 1368
Alw44I GTGCAC 1 cut(s) 1305
AlwI GGATC 4 cut(s) 19, 30, 43, 119
AoxI GGCC 4 cut(s) 304, 890, 911, 1414
ApaLI GTGCAC 1 cut(s) 1305
ApoI RAATTY 1 cut(s) 158
AsiGI ACCGGT 1 cut(s) 757
AsiSI GCGATCGC 1 cut(s) 135
AspLEI GCGC 2 cut(s) 294, 805
AspS9I GGNCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
AsuC2I CCSGG 1 cut(s) 1156
AsuHPI GGTGA 5 cut(s) 575, 647, 833, 999, 1346
AsuNHI GCTAGC 2 cut(s) 484, 505
AvaII GGWCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
AxyI CCTNAGG 2 cut(s) 1135, 1465
BaeGI GKGCMC 2 cut(s) 765, 1309
BalI TGGCCA 2 cut(s) 892, 1416
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 760
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 1309
BbvCI CCTCAGC 2 cut(s) 1083, 1206
BccI CCATC 7 cut(s) 169, 787, 844, 1115, 1396, 1430, 1479
BcgI CGANNNNNNTGC 2 cut(s) 168, 202
BciT130I CCWGG 3 cut(s) 910, 957, 1283
BciVI GTATCC 2 cut(s) 1043, 1256
BcnI CCSGG 1 cut(s) 1156
BcoDI GTCTC 4 cut(s) 327, 465, 552, 1368
BfaI CTAG 3 cut(s) 485, 506, 1352
BfuAI ACCTGC 2 cut(s) 760, 1277
BfuI GTATCC 2 cut(s) 1043, 1256
BglII AGATCT 1 cut(s) 419
BlpI GCTNAGC 1 cut(s) 850
Bme1390I CCNGG 4 cut(s) 910, 957, 1156, 1283
Bme18I GGWCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
BmgT120I GGNCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
BmiI GGNNCC 4 cut(s) 37, 58, 762, 1077
BmrFI CCNGG 4 cut(s) 910, 957, 1156, 1283
BmsI GCATC 4 cut(s) 279, 391, 610, 990
BmtI GCTAGC 2 cut(s) 488, 509
BpiI GAAGAC 1 cut(s) 177
BplI GAGNNNNNCTC 2 cut(s) 599, 631
Bpu10I CCTNAGC 2 cut(s) 1083, 1206
Bpu1102I GCTNAGC 1 cut(s) 850
BpuMI CCSGG 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 1125
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1368
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 1365, 1395
Bsc4I CCNNNNNNNGG 7 cut(s) 1016, 1115, 1154, 1256, 1384, 1481, 1485
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 3 cut(s) 893, 971, 1120
Bse21I CCTNAGG 2 cut(s) 1135, 1465
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 3 cut(s) 910, 957, 1283
BseGI GGATG 4 cut(s) 180, 406, 1407, 1490
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 7 cut(s) 1016, 1115, 1154, 1256, 1384, 1481, 1485
BseNI ACTGG 3 cut(s) 893, 971, 1120
BseSI GKGCMC 2 cut(s) 765, 1309
BseYI CCCAGC 1 cut(s) 1272
BsgI GTGCAG 5 cut(s) 545, 701, 738, 933, 1044
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 4 cut(s) 306, 892, 913, 1416
BshNI GGYRCC 1 cut(s) 760
BshTI ACCGGT 1 cut(s) 757
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 1309
BsiSI CCGG 2 cut(s) 758, 1155
BslI CCNNNNNNNGG 7 cut(s) 1016, 1115, 1154, 1256, 1384, 1481, 1485
BsmAI GTCTC 4 cut(s) 327, 465, 552, 1368
BsmBI CGTCTC 1 cut(s) 552
BsmI GAATGC 1 cut(s) 1194
BsnI GGCC 4 cut(s) 306, 892, 913, 1416
Bso31I GGTCTC 1 cut(s) 1368
Bsp1286I GDGCHC 2 cut(s) 765, 1309
Bsp143I GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
Bsp1720I GCTNAGC 1 cut(s) 850
BspACI CCGC 7 cut(s) 686, 872, 968, 1211, 1277, 1293, 1399
BspANI GGCC 4 cut(s) 306, 892, 913, 1416
BspLI GGNNCC 4 cut(s) 37, 58, 762, 1077
BspMI ACCTGC 2 cut(s) 760, 1277
BspOI GCTAGC 2 cut(s) 488, 509
BspPI GGATC 4 cut(s) 19, 30, 43, 119
BspQI GCTCTTC 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 760
BspTNI GGTCTC 1 cut(s) 1368
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 3 cut(s) 893, 971, 1120
BssAI RCCGGY 1 cut(s) 757
BssMI GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 3 cut(s) 910, 957, 1283
Bst4CI ACNGT 7 cut(s) 212, 253, 572, 918, 1032, 1055, 1334
Bst6I CTCTTC 2 cut(s) 596, 1401
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 7 cut(s) 486, 507, 639, 948, 995, 1041, 1230
BstENI CCTNNNNNAGG 1 cut(s) 1479
BstF5I GGATG 4 cut(s) 180, 406, 1407, 1490
BstHHI GCGC 2 cut(s) 294, 805
BstKTI GATC 7 cut(s) 14, 38, 127, 135, 377, 422, 1325
BstMAI GTCTC 4 cut(s) 327, 465, 552, 1368
BstMBI GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
BstMCI CGRYCG 1 cut(s) 135
BstNI CCWGG 3 cut(s) 910, 957, 1283
BstNSI RCATGY 2 cut(s) 721, 997
BstSCI CCNGG 4 cut(s) 908, 955, 1154, 1281
BstSLI GKGCMC 2 cut(s) 765, 1309
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 3 cut(s) 11, 35, 419
BstXI CCANNNNNNTGG 1 cut(s) 1289
BstYI RGATCY 3 cut(s) 11, 35, 419
Bsu36I CCTNAGG 2 cut(s) 1135, 1465
BsuI GTATCC 2 cut(s) 1043, 1256
BsuRI GGCC 4 cut(s) 306, 892, 913, 1416
BtgZI GCGATG 1 cut(s) 1134
BtsCI GGATG 4 cut(s) 180, 406, 1407, 1490
BtsIMutI CAGTG 1 cut(s) 249
BveI ACCTGC 2 cut(s) 760, 1277
Cac8I GCNNGC 7 cut(s) 486, 507, 639, 948, 995, 1041, 1230
CfoI GCGC 2 cut(s) 294, 805
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
Csp6I GTAC 1 cut(s) 877
CspAI ACCGGT 1 cut(s) 757
CspCI CAANNNNNGTGG 2 cut(s) 1299, 1334
CviQI GTAC 1 cut(s) 877
DpnI GATC 7 cut(s) 13, 37, 126, 134, 376, 421, 1324
DpnII GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
DraIII CACNNNGTG 1 cut(s) 1256
DrdI GACNNNNNNGTC 1 cut(s) 1096
DseDI GACNNNNNNGTC 1 cut(s) 1096
EaeI YGGCCR 2 cut(s) 890, 1414
Eam1104I CTCTTC 2 cut(s) 596, 1401
EarI CTCTTC 2 cut(s) 596, 1401
Eco147I AGGCCT 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 1368
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
Eco81I CCTNAGG 2 cut(s) 1135, 1465
EcoNI CCTNNNNNAGG 1 cut(s) 1479
EcoO109I RGGNCCY 2 cut(s) 1139, 1472
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 3 cut(s) 908, 955, 1281
EcoRV GATATC 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 552
FauI CCCGC 1 cut(s) 879
FokI GGATG 3 cut(s) 187, 413, 1414
FspBI CTAG 3 cut(s) 485, 506, 1352
GlaI GCGC 2 cut(s) 293, 804
GsaI CCCAGC 1 cut(s) 1276
HaeIII GGCC 4 cut(s) 306, 892, 913, 1416
HapII CCGG 2 cut(s) 758, 1155
HhaI GCGC 2 cut(s) 294, 805
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 292, 803
HinP1I GCGC 2 cut(s) 292, 803
HincII GTYRAC 2 cut(s) 28, 384
HindII GTYRAC 2 cut(s) 28, 384
HindIII AAGCTT 2 cut(s) 413, 1041
HinfI GANTC 7 cut(s) 101, 224, 385, 463, 533, 808, 977
HpaII CCGG 2 cut(s) 758, 1155
HphI GGTGA 5 cut(s) 575, 647, 833, 999, 1346
Hpy166II GTNNAC 7 cut(s) 28, 215, 314, 384, 1250, 1307, 1337
Hpy188I TCNGA 6 cut(s) 61, 124, 379, 498, 619, 1370
Hpy8I GTNNAC 7 cut(s) 28, 215, 314, 384, 1250, 1307, 1337
Hpy99I CGWCG 1 cut(s) 547
HpyAV CCTTC 3 cut(s) 296, 1161, 1172
HpyCH4III ACNGT 7 cut(s) 212, 253, 572, 918, 1032, 1055, 1334
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 292, 803
Kzo9I GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 3 cut(s) 62, 838, 1081
LweI GCATC 4 cut(s) 279, 391, 610, 990
MaeI CTAG 3 cut(s) 485, 506, 1352
MaeIII GTNAC 1 cut(s) 1295
MalI GATC 7 cut(s) 13, 37, 126, 134, 376, 421, 1324
MboI GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
MboII GAAGA 7 cut(s) 6, 177, 280, 428, 531, 613, 1418
MflI RGATCY 3 cut(s) 11, 35, 419
MhlI GDGCHC 2 cut(s) 765, 1309
MlsI TGGCCA 2 cut(s) 892, 1416
MluCI AATT 1 cut(s) 158
MluNI TGGCCA 2 cut(s) 892, 1416
MlyI GAGTC 4 cut(s) 95, 379, 542, 817
MmeI TCCRAC 2 cut(s) 168, 174
Mox20I TGGCCA 2 cut(s) 892, 1416
MscI TGGCCA 2 cut(s) 892, 1416
MslI CAYNNNNRTG 4 cut(s) 269, 393, 399, 998
Msp20I TGGCCA 2 cut(s) 892, 1416
MspA1I CMGCKG 1 cut(s) 1399
MspI CCGG 2 cut(s) 758, 1155
MspR9I CCNGG 4 cut(s) 910, 957, 1156, 1283
Mva1269I GAATGC 1 cut(s) 1194
MvaI CCWGG 3 cut(s) 910, 957, 1283
NciI CCSGG 1 cut(s) 1156
NdeII GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
NheI GCTAGC 2 cut(s) 484, 505
NlaIV GGNNCC 4 cut(s) 37, 58, 762, 1077
NspI RCATGY 2 cut(s) 721, 997
PaeI GCATGC 1 cut(s) 997
PaqCI CACCTGC 2 cut(s) 760, 1277
PceI AGGCCT 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 596
PctI GAATGC 1 cut(s) 1194
PfeI GAWTC 3 cut(s) 224, 463, 977
PflFI GACNNNGTC 3 cut(s) 251, 572, 1379
PflMI CCANNNNNTGG 4 cut(s) 1016, 1256, 1384, 1485
PinAI ACCGGT 1 cut(s) 757
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 4 cut(s) 95, 379, 541, 816
PpsI GAGTC 4 cut(s) 95, 379, 541, 816
PpuMI RGGWCCY 2 cut(s) 1139, 1472
Psp5II RGGWCCY 2 cut(s) 1139, 1472
Psp6I CCWGG 3 cut(s) 908, 955, 1281
PspFI CCCAGC 1 cut(s) 1272
PspGI CCWGG 3 cut(s) 908, 955, 1281
PspN4I GGNNCC 4 cut(s) 37, 58, 762, 1077
PspPI GGNCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
PspPPI RGGWCCY 2 cut(s) 1139, 1472
PsuI RGATCY 3 cut(s) 11, 35, 419
PsyI GACNNNGTC 3 cut(s) 251, 572, 1379
PvuI CGATCG 1 cut(s) 135
RgaI GCGATCGC 1 cut(s) 135
RsaI GTAC 1 cut(s) 878
RsaNI GTAC 1 cut(s) 877
RseI CAYNNNNRTG 4 cut(s) 269, 393, 399, 998
SapI GCTCTTC 1 cut(s) 596
Sau3AI GATC 7 cut(s) 11, 35, 124, 132, 374, 419, 1322
Sau96I GGNCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
SchI GAGTC 4 cut(s) 95, 379, 542, 817
ScrFI CCNGG 4 cut(s) 910, 957, 1156, 1283
SduI GDGCHC 2 cut(s) 765, 1309
SfaAI GCGATCGC 1 cut(s) 135
SfaNI GCATC 4 cut(s) 279, 391, 610, 990
SgfI GCGATCGC 1 cut(s) 135
SgrAI CRCCGGYG 1 cut(s) 757
SinI GGWCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
SmiMI CAYNNNNRTG 4 cut(s) 269, 393, 399, 998
SphI GCATGC 1 cut(s) 997
Sse9I AATT 1 cut(s) 158
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 7 cut(s) 686, 872, 968, 1211, 1277, 1293, 1399
SspMI CTAG 3 cut(s) 485, 506, 1352
StuI AGGCCT 1 cut(s) 306
StyD4I CCNGG 4 cut(s) 908, 955, 1154, 1281
TaaI ACNGT 7 cut(s) 212, 253, 572, 918, 1032, 1055, 1334
TaqI TCGA 5 cut(s) 48, 255, 426, 1425, 1443
TasI AATT 1 cut(s) 158
TauI GCSGC 3 cut(s) 688, 1213, 1279
TfiI GAWTC 3 cut(s) 224, 463, 977
TscAI CASTG 1 cut(s) 256
TspDTI ATGAA 3 cut(s) 281, 805, 1191
TspGWI ACGGA 1 cut(s) 1062
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 3 cut(s) 251, 572, 1379
Van91I CCANNNNNTGG 4 cut(s) 1016, 1256, 1384, 1485
VneI GTGCAC 1 cut(s) 1305
VpaK11BI GGWCC 7 cut(s) 113, 261, 568, 1019, 1139, 1247, 1472
XagI CCTNNNNNAGG 1 cut(s) 1479
XapI RAATTY 1 cut(s) 158
XceI RCATGY 2 cut(s) 721, 997
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XspI CTAG 3 cut(s) 485, 506, 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.