Rh2AG320000

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010117
Physical Location & Seq
Forward (+)
45821830 .. 45825356
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG320000.1

Sequence Viewer

Length: 1557 bp
ATGTGCTACCTTTACCAGGATTTTCACAAAAGTAGAATGGCAAGATCACCAATGTACACTTCTGCTGCCTATGGCCAGCCTGAAGACTCTCTCAACCAAGAAGTAATTGCTACTGTTGAAAAGAGCATGAAAAAATATGCTGACAATCTCATGCGCTTCCTTGAAGGAATTAGTTCACGGCTGTCACAGTTGGAATTATATTGCTACAATGTTGATAAGTCAATCGGCGAGATGCGTTCTGATTTAAATCGTGATCATGGGGAGGCAGATTCAAAGCTCAAGTCTCTTGAGAAACATCTGCAAGAAGTCCATAGGTCTGTCCAGATCCTGAGAGACAAGCAAGAACTTGCTGAGACACAAAAAGAATTAGCCAAGCTTCAGCTTGCACAGAAAGGATCTTCTTCATCGACCCATTCACAATCTAATGAGGAAAGAGCTTCACCACCTACCTCAGATGGTCAGAAGACTGACAGCACATCTGAACATAACCAGCAATTAGCTCTTGCCTTGCCCCATCAAGTAGCACCACAACAACAGCCGGTGGCACCTCCCCCACAGGCGCCAACTCAGAATGTGACCCAACCACAACAGCCCTATTATTTGCCTCAAACTCAGCTATCAAATCCACCACCCCCACAACAGCATCCTCAGAGTCAATATTTGGCTTCGGATCCTCAGTATCGAACCCCGCAACTGCAAGACCCTAGAGTGGCACCACAGCCAACACAATCTCAAGTAAATCAACCACAAGTCCAACCGTTCCCTCAGTATCCACAGCAATTACCACAGCAGTTACCTCAGCAGGTGCAGTATCCACAACAGCCCTCTGTGCAGCCCCAGATGAGGCCCCCATCAACACCTGTGTACCCACCATACCCACCTGGTCAACAGGCAAACCCACCATACCCACCTGGTCAACAGGCAAACCCACCATACCCACCTGGTCAACAGGCGAACCCACCATACCCATCTGGTCAACAGACGAACCCATCTCCTCCAGAGACTCTGCCAAACAGCATGCCAATGCAAGTATCGTATTCGAGTGTTCCTCAACCTGGTTCTGGTCGATCTGATGCCATGCCTTTTGGATATAGCGGAGCTGGTAGAACAATGCCACAGCAGCCTCCACCTCAGCAGATCAAGGGTGCTTTTGGAGCTCAACAAGGTGATGTGTATGCCTCTGCTGGGCCTCACCCTGCACTTCCCCCAGGGAGTACATATATGATGTATGATAGTGAAGGAGGTAGAAATCATTATTCTGCTCAGGTGCCTCATTACAGTCAAGGTGGATATCCTCCAGCAAGTGCCTCTCAGACTCCACAACCAACAACAGCTCCAAATCATATGGTTCGGAATCCGCAATTCATTCGTAACCATCCTTACAATGAGTTGATTGAGAAGTTGGTGAACATGGGTTTTAGGGGTGATCATGTTGCAAGTGTAATCCAGAGGATGGAGGAAAGTGGGCAAACCATCGATTTTAATGCTGTGATTGACAGGTTGAATGTACATTCATCTGGGGCTCCTCCTCCTCCTCAGAGAGGATGGTCAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

518

Amino Acids

57.31

Weight (kDa)

6.57

Isoelectric Point (pI)

72.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 461 - 505 4.7e-23 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 793
Acc36I ACCTGC 1 cut(s) 793
AccB1I GGYRCC 4 cut(s) 544, 559, 712, 1267
AccB7I CCANNNNNTGG 1 cut(s) 1453
AciI CCGC 3 cut(s) 689, 1095, 1358
AclWI GGATC 4 cut(s) 319, 403, 665, 678
AcoI YGGCCR 1 cut(s) 73
AcuI CTGAAG 2 cut(s) 102, 362
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 4 cut(s) 56, 866, 1216, 1509
AfiI CCNNNNNNNGG 8 cut(s) 16, 709, 843, 1055, 1061, 1185, 1453, 1541
AgsI TTSAA 4 cut(s) 119, 164, 273, 1504
AjnI CCWGG 6 cut(s) 15, 880, 910, 940, 1054, 1207
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 9 cut(s) 277, 376, 382, 437, 500, 616, 1100, 1157, 1334
AluI AGCT 9 cut(s) 277, 376, 382, 437, 500, 616, 1100, 1157, 1334
Alw21I GWGCWC 1 cut(s) 1159
Alw26I GTCTC 4 cut(s) 288, 327, 347, 995
AlwI GGATC 4 cut(s) 319, 403, 665, 678
AlwNI CAGNNNCTG 1 cut(s) 328
AoxI GGCC 3 cut(s) 73, 845, 1187
ApeKI GCWGC 3 cut(s) 65, 832, 1120
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
Asp700I GAANNNNTTC 1 cut(s) 172
AspLEI GCGC 2 cut(s) 156, 562
AspS9I GGNCC 2 cut(s) 846, 1187
AsuHPI GGTGA 6 cut(s) 39, 432, 1178, 1184, 1417, 1436
BaeI ACNNNNGTAYC 2 cut(s) 848, 881
BalI TGGCCA 1 cut(s) 75
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 4 cut(s) 544, 559, 712, 1267
BanII GRGCYC 2 cut(s) 1159, 1525
BbsI GAAGAC 2 cut(s) 90, 470
Bbv12I GWGCWC 1 cut(s) 1159
BbvCI CCTCAGC 2 cut(s) 798, 1131
BbvI GCAGC 3 cut(s) 52, 844, 1132
BccI CCATC 9 cut(s) 449, 522, 859, 976, 997, 1383, 1447, 1481, 1539
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 4 cut(s) 1349, 1383, 1466, 1500
BciT130I CCWGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
BciVI GTATCC 2 cut(s) 780, 822
BclI TGATCA 2 cut(s) 253, 1426
BcoDI GTCTC 4 cut(s) 288, 327, 347, 995
BfaI CTAG 1 cut(s) 705
BfoI RGCGCY 1 cut(s) 563
BfuAI ACCTGC 1 cut(s) 793
BfuI GTATCC 2 cut(s) 780, 822
BisI GCNGC 3 cut(s) 66, 833, 1121
BlsI GCNGC 3 cut(s) 67, 834, 1122
Bme1390I CCNGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
BmgT120I GGNCC 2 cut(s) 846, 1187
BmiI GGNNCC 7 cut(s) 546, 561, 672, 714, 848, 1269, 1524
BmrFI CCNGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
BmsI GCATC 3 cut(s) 222, 652, 1063
BpiI GAAGAC 2 cut(s) 90, 470
BplI GAGNNNNNCTC 2 cut(s) 1033, 1065
BpmI CTGGAG 2 cut(s) 981, 1281
Bpu10I CCTNAGC 3 cut(s) 798, 1131, 1263
BpuEI CTTGAG 3 cut(s) 263, 308, 717
Bsa29I ATCGAT 1 cut(s) 1476
BsaBI GATNNNNATC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 1207, 1208
BsaXI ACNNNNNCTCC 2 cut(s) 1318, 1348
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 709, 843, 1055, 1061, 1185, 1453, 1541
Bse118I RCCGGY 1 cut(s) 538
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
BseCI ATCGAT 1 cut(s) 1476
BseDI CCNNGG 2 cut(s) 1207, 1208
BseGI GGATG 4 cut(s) 643, 1375, 1458, 1550
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 8 cut(s) 16, 709, 843, 1055, 1061, 1185, 1453, 1541
BseRI GAGGAG 5 cut(s) 984, 1515, 1518, 1521, 1524
BseXI GCAGC 3 cut(s) 52, 844, 1132
BseYI CCCAGC 1 cut(s) 1184
BsgI GTGCAG 3 cut(s) 827, 851, 1182
BshFI GGCC 3 cut(s) 75, 847, 1189
BshNI GGYRCC 4 cut(s) 544, 559, 712, 1267
BshVI ATCGAT 1 cut(s) 1476
BsiHKAI GWGCWC 1 cut(s) 1159
BsiSI CCGG 1 cut(s) 539
BslI CCNNNNNNNGG 8 cut(s) 16, 709, 843, 1055, 1061, 1185, 1453, 1541
BsmAI GTCTC 4 cut(s) 288, 327, 347, 995
BsnI GGCC 3 cut(s) 75, 847, 1189
Bsp1286I GDGCHC 2 cut(s) 1159, 1525
Bsp1407I TGTACA 2 cut(s) 54, 1507
Bsp143I GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
BspACI CCGC 3 cut(s) 689, 1095, 1358
BspANI GGCC 3 cut(s) 75, 847, 1189
BspDI ATCGAT 1 cut(s) 1476
BspLI GGNNCC 7 cut(s) 546, 561, 672, 714, 848, 1269, 1524
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 4 cut(s) 319, 403, 665, 678
BspT107I GGYRCC 4 cut(s) 544, 559, 712, 1267
BsrFI RCCGGY 1 cut(s) 538
BsrGI TGTACA 2 cut(s) 54, 1507
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 1207, 1208
BssMI GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
BssNI GRCGYC 1 cut(s) 560
Bst2UI CCWGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
Bst4CI ACNGT 4 cut(s) 115, 189, 759, 1280
BstACI GRCGYC 1 cut(s) 560
BstAUI TGTACA 2 cut(s) 54, 1507
BstC8I GCNNGC 3 cut(s) 77, 384, 1019
BstENI CCTNNNNNAGG 2 cut(s) 14, 1539
BstF5I GGATG 4 cut(s) 643, 1375, 1458, 1550
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 2 cut(s) 156, 562
BstKTI GATC 8 cut(s) 47, 256, 327, 398, 673, 1070, 1140, 1429
BstMAI GTCTC 4 cut(s) 288, 327, 347, 995
BstMBI GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
BstMWI GCNNNNNNNGC 3 cut(s) 829, 1120, 1154
BstNI CCWGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
BstNSI RCATGY 1 cut(s) 1021
BstSCI CCNGG 6 cut(s) 15, 880, 910, 940, 1054, 1207
BstV1I GCAGC 3 cut(s) 52, 844, 1132
BstV2I GAAGAC 2 cut(s) 90, 470
BstX2I RGATCY 3 cut(s) 324, 395, 670
BstYI RGATCY 3 cut(s) 324, 395, 670
Bsu15I ATCGAT 1 cut(s) 1476
BsuI GTATCC 2 cut(s) 780, 822
BsuRI GGCC 3 cut(s) 75, 847, 1189
BsuTUI ATCGAT 1 cut(s) 1476
BtsCI GGATG 4 cut(s) 643, 1375, 1458, 1550
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 3 cut(s) 77, 384, 1019
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 2 cut(s) 156, 562
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 2 cut(s) 846, 1187
ClaI ATCGAT 1 cut(s) 1476
CsiI ACCWGGT 4 cut(s) 880, 910, 940, 1054
Csp6I GTAC 4 cut(s) 55, 865, 1215, 1508
CviAII CATG 7 cut(s) 127, 151, 257, 1018, 1078, 1411, 1430
CviQI GTAC 4 cut(s) 55, 865, 1215, 1508
DinI GGCGCC 1 cut(s) 561
DpnI GATC 8 cut(s) 46, 255, 326, 397, 672, 1069, 1139, 1428
DpnII GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
DraI TTTAAA 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 1157
Eco24I GRGCYC 2 cut(s) 1159, 1525
Eco32I GATATC 1 cut(s) 1292
Eco53kI GAGCTC 1 cut(s) 1157
Eco57I CTGAAG 2 cut(s) 102, 362
EcoICRI GAGCTC 1 cut(s) 1157
EcoNI CCTNNNNNAGG 2 cut(s) 14, 1539
EcoO109I RGGNCCY 1 cut(s) 846
EcoRII CCWGG 6 cut(s) 15, 880, 910, 940, 1054, 1207
EcoRV GATATC 1 cut(s) 1292
EcoT38I GRGCYC 2 cut(s) 1159, 1525
EgeI GGCGCC 1 cut(s) 561
EheI GGCGCC 1 cut(s) 561
FaeI CATG 7 cut(s) 130, 154, 260, 1021, 1081, 1414, 1433
FatI CATG 7 cut(s) 126, 150, 256, 1017, 1077, 1410, 1429
FauI CCCGC 1 cut(s) 696
FauNDI CATATG 1 cut(s) 1344
FbaI TGATCA 2 cut(s) 253, 1426
Fnu4HI GCNGC 3 cut(s) 66, 833, 1121
FokI GGATG 3 cut(s) 630, 1362, 1465
FriOI GRGCYC 2 cut(s) 1159, 1525
Fsp4HI GCNGC 3 cut(s) 66, 833, 1121
FspBI CTAG 1 cut(s) 705
GlaI GCGC 2 cut(s) 155, 561
GluI GCNGC 3 cut(s) 66, 833, 1121
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 2 cut(s) 981, 1281
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 3 cut(s) 75, 847, 1189
HapII CCGG 1 cut(s) 539
HhaI GCGC 2 cut(s) 156, 562
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 7 cut(s) 130, 154, 260, 1021, 1081, 1414, 1433
Hin6I GCGC 2 cut(s) 154, 560
HinP1I GCGC 2 cut(s) 154, 560
HincII GTYRAC 4 cut(s) 887, 917, 947, 977
HindII GTYRAC 4 cut(s) 887, 917, 947, 977
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 6 cut(s) 86, 269, 652, 1003, 1315, 1354
HpaII CCGG 1 cut(s) 539
HphI GGTGA 6 cut(s) 39, 432, 1178, 1184, 1417, 1436
Hpy166II GTNNAC 8 cut(s) 57, 176, 865, 887, 917, 947, 977, 1408
Hpy188III TCNNGA 6 cut(s) 251, 287, 322, 328, 998, 1447
Hpy8I GTNNAC 8 cut(s) 57, 176, 865, 887, 917, 947, 977, 1408
HpyAV CCTTC 2 cut(s) 158, 1232
HpyCH4III ACNGT 4 cut(s) 115, 189, 759, 1280
HpyCH4V TGCA 8 cut(s) 301, 386, 697, 808, 832, 1027, 1199, 1436
HpyF10VI GCNNNNNNNGC 3 cut(s) 829, 1120, 1154
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 7 cut(s) 130, 154, 260, 1021, 1081, 1414, 1433
HspAI GCGC 2 cut(s) 154, 560
KasI GGCGCC 1 cut(s) 559
Ksp22I TGATCA 2 cut(s) 253, 1426
Kzo9I GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
LmnI GCTCC 4 cut(s) 1097, 1154, 1339, 1528
Lsp1109I GCAGC 3 cut(s) 52, 844, 1132
LweI GCATC 3 cut(s) 222, 652, 1063
MabI ACCWGGT 4 cut(s) 880, 910, 940, 1054
MaeI CTAG 1 cut(s) 705
MaeIII GTNAC 4 cut(s) 183, 574, 792, 1370
MalI GATC 8 cut(s) 46, 255, 326, 397, 672, 1069, 1139, 1428
MboI GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
MboII GAAGA 4 cut(s) 95, 390, 393, 475
MflI RGATCY 3 cut(s) 324, 395, 670
MhlI GDGCHC 2 cut(s) 1159, 1525
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 7 cut(s) 105, 168, 194, 365, 494, 779, 1361
MluNI TGGCCA 1 cut(s) 75
Mly113I GGCGCC 1 cut(s) 560
MlyI GAGTC 4 cut(s) 80, 661, 997, 1309
MmeI TCCRAC 2 cut(s) 171, 778
Mox20I TGGCCA 1 cut(s) 75
MroXI GAANNNNTTC 1 cut(s) 172
MscI TGGCCA 1 cut(s) 75
MseI TTAA 2 cut(s) 245, 1482
MslI CAYNNNNRTG 1 cut(s) 1022
Msp20I TGGCCA 1 cut(s) 75
MspI CCGG 1 cut(s) 539
MspR9I CCNGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
MvaI CCWGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
MwoI GCNNNNNNNGC 3 cut(s) 829, 1120, 1154
NarI GGCGCC 1 cut(s) 560
NdeI CATATG 1 cut(s) 1344
NdeII GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
NlaIII CATG 7 cut(s) 130, 154, 260, 1021, 1081, 1414, 1433
NlaIV GGNNCC 7 cut(s) 546, 561, 672, 714, 848, 1269, 1524
NmuCI GTSAC 2 cut(s) 183, 574
NspI RCATGY 1 cut(s) 1021
PaeI GCATGC 1 cut(s) 1021
PaqCI CACCTGC 1 cut(s) 793
PasI CCCWGGG 1 cut(s) 1208
PdmI GAANNNNTTC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 269, 1354
PflMI CCANNNNNTGG 1 cut(s) 1453
PkrI GCNGC 3 cut(s) 67, 834, 1122
PleI GAGTC 4 cut(s) 80, 660, 997, 1309
PluTI GGCGCC 1 cut(s) 563
PpsI GAGTC 4 cut(s) 80, 660, 997, 1309
Psp124BI GAGCTC 1 cut(s) 1159
Psp6I CCWGG 6 cut(s) 15, 880, 910, 940, 1054, 1207
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 6 cut(s) 15, 880, 910, 940, 1054, 1207
PspN4I GGNNCC 7 cut(s) 546, 561, 672, 714, 848, 1269, 1524
PspPI GGNCC 2 cut(s) 846, 1187
PsrI GAACNNNNNNTAC 2 cut(s) 947, 979
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 3 cut(s) 324, 395, 670
RsaI GTAC 4 cut(s) 56, 866, 1216, 1509
RsaNI GTAC 4 cut(s) 55, 865, 1215, 1508
RseI CAYNNNNRTG 1 cut(s) 1022
SacI GAGCTC 1 cut(s) 1159
SaqAI TTAA 2 cut(s) 245, 1482
SatI GCNGC 3 cut(s) 66, 833, 1121
Sau3AI GATC 8 cut(s) 44, 253, 324, 395, 670, 1067, 1137, 1426
Sau96I GGNCC 2 cut(s) 846, 1187
SchI GAGTC 4 cut(s) 80, 661, 997, 1309
ScrFI CCNGG 6 cut(s) 17, 882, 912, 942, 1056, 1209
SduI GDGCHC 2 cut(s) 1159, 1525
SexAI ACCWGGT 4 cut(s) 880, 910, 940, 1054
SfaNI GCATC 3 cut(s) 222, 652, 1063
SfoI GGCGCC 1 cut(s) 561
SmiI ATTTAAAT 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 3 cut(s) 278, 287, 732
SmoI CTYRAG 3 cut(s) 278, 287, 732
SphI GCATGC 1 cut(s) 1021
Sse9I AATT 7 cut(s) 105, 168, 194, 365, 494, 779, 1361
SsiI CCGC 3 cut(s) 689, 1095, 1358
SspDI GGCGCC 1 cut(s) 559
SspI AATATT 1 cut(s) 659
SspMI CTAG 1 cut(s) 705
SstI GAGCTC 1 cut(s) 1159
StyD4I CCNGG 6 cut(s) 15, 880, 910, 940, 1054, 1207
SwaI ATTTAAAT 1 cut(s) 246
TaaI ACNGT 4 cut(s) 115, 189, 759, 1280
TaqI TCGA 5 cut(s) 407, 682, 1040, 1066, 1476
TasI AATT 7 cut(s) 105, 168, 194, 365, 494, 779, 1361
TatI WGTACW 3 cut(s) 54, 1214, 1507
TfiI GAWTC 2 cut(s) 269, 1354
Tru1I TTAA 2 cut(s) 245, 1482
Tru9I TTAA 2 cut(s) 245, 1482
TseFI GTSAC 2 cut(s) 183, 574
TseI GCWGC 3 cut(s) 65, 832, 1120
Tsp45I GTSAC 2 cut(s) 183, 574
TspDTI ATGAA 4 cut(s) 143, 393, 1354, 1503
Van91I CCANNNNNTGG 1 cut(s) 1453
XagI CCTNNNNNAGG 2 cut(s) 14, 1539
XceI RCATGY 1 cut(s) 1021
XmnI GAANNNNTTC 1 cut(s) 172
XspI CTAG 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.