Rh2BG328700

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010117
Physical Location & Seq
Forward (+)
42861129 .. 42865691
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG328700.1

Sequence Viewer

Length: 1527 bp
ATGTGCTACCTTTACCAGGATTTTCACAAAAGTAGAATGGCAAGATCACCAATGTACACTTCTGCTGCCTATGGCCAGCCTGAAGACTCTCTCAACCAAGAAGTAATTGCTACTGTTGAAAAAAGCATGAAAAAATATGCTGACAATCTCATGCGCTTCCTTGAAGGAATTAGTTCACGGCTGTCACAGTTGGAATTATATTGCTACAATGTTGATAAGTCAATCGGTGAGATGCGTTCTGATTTAAATCGTGATCATGGGGAGGCAGATTCAAAGCTCAAGTCTCTTGAGAAACATCTGCAAGAAGTCCATAGGTCTGTCCAGATCCTGAGAGACAAGCAAGAACTTGCTGAGACTCAAAAAGAATTAGCCAAGCTTCAGCTTGCACAGAAAGGATCTTCTTCATCAACCCATTCACAATCTAATGAGGAAAGAGCTTCACCACCTACCTCAGATGGTCAGAAGACTGACAACACATCTGAACATAACCAGCAATTAGCTCTTGCCTTGCCCCATCAAGTAGCACCACAACAACAGCCGGTGGCACCTCCCCCACAGGCGCCAACTCAGAATGTGACCCAACCACAACAGCCCTATTATTTACCTCAAACTCAGCTATCAAATCCACCACCCCCACAGCAGCATCCTCAGAGTCAATATTTGGCTTCGGATCCTCAGTATCGAACCCCGCAACTGCAAGACCCTAGAGTGGCACCACAGCCAACACAATCTCAAGTAAATCAACCACAAGTCCAACCGTTCCCTCAGTATCCACAGCAATTACCACAGCAGTTACCTCAGCAGGTGCAGTATCCACAACAGCCCTCTGTGCAGCCCCAGATGAGACCCCCATCAACACCTGTGTACCCACCATACCCTCCTGGTCAACAGGCGAACCCACCATACCCACCTGGTCAACAGGCGAACCCACCATACCCATCTGGTCAACAGACGAACCCATCTCCTCCAGAGACTCTGCCAAACAGCATGCCAATGCAAGTATCGTATTCAAGTGTTCCTCAACCTGGTTCTGGTCGTTCTGATGCCATGCCTTTTGGATATAGCGGAGCTGGTAGAACAATGCCACAGCAGCCTCCACCTCAGCAGATCAAGGGTGCTTTTGGAGCTCAACAAGGTGATGTGTATGCCTCTGCTGGGCCTCACCCTGCACTTCCCCCAGGGAGTACATATATGATGTATGATAGTGAAGGAGGTAGAAATCATTATTCTGCTCAGGTGCCTCATTACAGTCAAGGTGGATATCCTCCAGCAAGTGCCTCTCAGACTCCACAACCAACAACAGCTCCAAATCATATGGTTCGGAATCCGCAATTCATTCGTAACCATCCTTACAACGAGTTGATTGAGAAGTTGGTGAACATGGGTTTTAGGGGTGATCATGTTGCAAGTGTAATCCAGAGGATGGAGGAAAGTGGCCAAACCATCGATTTTAATGCTGTGATTGACAGGTTGAATGTACATTCATCTGGGGCTCCTCCTCCTCCTCAGAGAGGATGGTCAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1421

Protein Analysis

508

Amino Acids

56.29

Weight (kDa)

6.57

Isoelectric Point (pI)

71.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 451 - 495 4.6e-23 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 793
Acc36I ACCTGC 1 cut(s) 793
AccB1I GGYRCC 4 cut(s) 544, 559, 712, 1237
AccB7I CCANNNNNTGG 1 cut(s) 1423
AciI CCGC 3 cut(s) 689, 1065, 1328
AclWI GGATC 4 cut(s) 319, 403, 665, 678
AcoI YGGCCR 2 cut(s) 73, 1435
AcuI CTGAAG 2 cut(s) 102, 362
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 4 cut(s) 56, 866, 1186, 1479
AfiI CCNNNNNNNGG 7 cut(s) 16, 709, 1025, 1031, 1155, 1423, 1511
AgsI TTSAA 5 cut(s) 119, 164, 273, 1011, 1474
AjnI CCWGG 5 cut(s) 15, 880, 910, 1024, 1177
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 9 cut(s) 277, 376, 382, 437, 500, 616, 1070, 1127, 1304
AluI AGCT 9 cut(s) 277, 376, 382, 437, 500, 616, 1070, 1127, 1304
Alw21I GWGCWC 1 cut(s) 1129
Alw26I GTCTC 5 cut(s) 288, 327, 347, 838, 965
AlwI GGATC 4 cut(s) 319, 403, 665, 678
AlwNI CAGNNNCTG 1 cut(s) 328
AoxI GGCC 3 cut(s) 73, 1157, 1435
ApeKI GCWGC 4 cut(s) 65, 640, 832, 1090
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
Asp700I GAANNNNTTC 1 cut(s) 172
AspLEI GCGC 2 cut(s) 156, 562
AspS9I GGNCC 1 cut(s) 1157
AsuHPI GGTGA 7 cut(s) 39, 239, 432, 1148, 1154, 1387, 1406
BaeI ACNNNNGTAYC 2 cut(s) 848, 881
BalI TGGCCA 2 cut(s) 75, 1437
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 4 cut(s) 544, 559, 712, 1237
BanII GRGCYC 2 cut(s) 1129, 1495
BbsI GAAGAC 2 cut(s) 90, 470
Bbv12I GWGCWC 1 cut(s) 1129
BbvCI CCTCAGC 2 cut(s) 798, 1101
BbvI GCAGC 4 cut(s) 52, 652, 844, 1102
BccI CCATC 9 cut(s) 449, 522, 859, 946, 967, 1353, 1417, 1451, 1509
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 4 cut(s) 1319, 1353, 1436, 1470
BciT130I CCWGG 5 cut(s) 17, 882, 912, 1026, 1179
BciVI GTATCC 2 cut(s) 780, 822
BclI TGATCA 2 cut(s) 253, 1396
BcoDI GTCTC 5 cut(s) 288, 327, 347, 838, 965
BfaI CTAG 1 cut(s) 705
BfoI RGCGCY 1 cut(s) 563
BfuAI ACCTGC 1 cut(s) 793
BfuI GTATCC 2 cut(s) 780, 822
BisI GCNGC 4 cut(s) 66, 641, 833, 1091
BlsI GCNGC 4 cut(s) 67, 642, 834, 1092
Bme1390I CCNGG 5 cut(s) 17, 882, 912, 1026, 1179
BmgT120I GGNCC 1 cut(s) 1157
BmiI GGNNCC 6 cut(s) 546, 561, 672, 714, 1239, 1494
BmrFI CCNGG 5 cut(s) 17, 882, 912, 1026, 1179
BmsI GCATC 3 cut(s) 222, 652, 1033
BpiI GAAGAC 2 cut(s) 90, 470
BpmI CTGGAG 2 cut(s) 951, 1251
Bpu10I CCTNAGC 3 cut(s) 798, 1101, 1233
BpuEI CTTGAG 3 cut(s) 263, 308, 717
Bsa29I ATCGAT 1 cut(s) 1446
BsaBI GATNNNNATC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 2 cut(s) 1177, 1178
BsaXI ACNNNNNCTCC 2 cut(s) 1288, 1318
Bsc4I CCNNNNNNNGG 7 cut(s) 16, 709, 1025, 1031, 1155, 1423, 1511
Bse118I RCCGGY 1 cut(s) 538
Bse8I GATNNNNATC 1 cut(s) 246
BseBI CCWGG 5 cut(s) 17, 882, 912, 1026, 1179
BseCI ATCGAT 1 cut(s) 1446
BseDI CCNNGG 2 cut(s) 1177, 1178
BseGI GGATG 4 cut(s) 643, 1345, 1428, 1520
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 7 cut(s) 16, 709, 1025, 1031, 1155, 1423, 1511
BseRI GAGGAG 5 cut(s) 954, 1485, 1488, 1491, 1494
BseXI GCAGC 4 cut(s) 52, 652, 844, 1102
BseYI CCCAGC 1 cut(s) 1154
BsgI GTGCAG 3 cut(s) 827, 851, 1152
BshFI GGCC 3 cut(s) 75, 1159, 1437
BshNI GGYRCC 4 cut(s) 544, 559, 712, 1237
BshVI ATCGAT 1 cut(s) 1446
BsiHKAI GWGCWC 1 cut(s) 1129
BsiSI CCGG 1 cut(s) 539
BslI CCNNNNNNNGG 7 cut(s) 16, 709, 1025, 1031, 1155, 1423, 1511
BsmAI GTCTC 5 cut(s) 288, 327, 347, 838, 965
BsnI GGCC 3 cut(s) 75, 1159, 1437
Bso31I GGTCTC 1 cut(s) 838
Bsp1286I GDGCHC 2 cut(s) 1129, 1495
Bsp1407I TGTACA 2 cut(s) 54, 1477
Bsp143I GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
BspACI CCGC 3 cut(s) 689, 1065, 1328
BspANI GGCC 3 cut(s) 75, 1159, 1437
BspDI ATCGAT 1 cut(s) 1446
BspLI GGNNCC 6 cut(s) 546, 561, 672, 714, 1239, 1494
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 4 cut(s) 319, 403, 665, 678
BspT107I GGYRCC 4 cut(s) 544, 559, 712, 1237
BspTNI GGTCTC 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 538
BsrGI TGTACA 2 cut(s) 54, 1477
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 1177, 1178
BssMI GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
BssNI GRCGYC 1 cut(s) 560
Bst2UI CCWGG 5 cut(s) 17, 882, 912, 1026, 1179
Bst4CI ACNGT 4 cut(s) 115, 189, 759, 1250
BstACI GRCGYC 1 cut(s) 560
BstAUI TGTACA 2 cut(s) 54, 1477
BstC8I GCNNGC 3 cut(s) 77, 384, 989
BstENI CCTNNNNNAGG 2 cut(s) 14, 1509
BstF5I GGATG 4 cut(s) 643, 1345, 1428, 1520
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 2 cut(s) 156, 562
BstKTI GATC 7 cut(s) 47, 256, 327, 398, 673, 1110, 1399
BstMAI GTCTC 5 cut(s) 288, 327, 347, 838, 965
BstMBI GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
BstMWI GCNNNNNNNGC 3 cut(s) 829, 1090, 1124
BstNI CCWGG 5 cut(s) 17, 882, 912, 1026, 1179
BstNSI RCATGY 1 cut(s) 991
BstSCI CCNGG 5 cut(s) 15, 880, 910, 1024, 1177
BstV1I GCAGC 4 cut(s) 52, 652, 844, 1102
BstV2I GAAGAC 2 cut(s) 90, 470
BstX2I RGATCY 3 cut(s) 324, 395, 670
BstYI RGATCY 3 cut(s) 324, 395, 670
Bsu15I ATCGAT 1 cut(s) 1446
BsuI GTATCC 2 cut(s) 780, 822
BsuRI GGCC 3 cut(s) 75, 1159, 1437
BsuTUI ATCGAT 1 cut(s) 1446
BtsCI GGATG 4 cut(s) 643, 1345, 1428, 1520
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 3 cut(s) 77, 384, 989
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 2 cut(s) 156, 562
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 1 cut(s) 1157
ClaI ATCGAT 1 cut(s) 1446
CsiI ACCWGGT 2 cut(s) 910, 1024
Csp6I GTAC 4 cut(s) 55, 865, 1185, 1478
CviAII CATG 7 cut(s) 127, 151, 257, 988, 1048, 1381, 1400
CviQI GTAC 4 cut(s) 55, 865, 1185, 1478
DinI GGCGCC 1 cut(s) 561
DpnI GATC 7 cut(s) 46, 255, 326, 397, 672, 1109, 1398
DpnII GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
DraI TTTAAA 1 cut(s) 246
EaeI YGGCCR 2 cut(s) 73, 1435
Ecl136II GAGCTC 1 cut(s) 1127
Eco24I GRGCYC 2 cut(s) 1129, 1495
Eco31I GGTCTC 1 cut(s) 838
Eco32I GATATC 1 cut(s) 1262
Eco53kI GAGCTC 1 cut(s) 1127
Eco57I CTGAAG 2 cut(s) 102, 362
EcoICRI GAGCTC 1 cut(s) 1127
EcoNI CCTNNNNNAGG 2 cut(s) 14, 1509
EcoRII CCWGG 5 cut(s) 15, 880, 910, 1024, 1177
EcoRV GATATC 1 cut(s) 1262
EcoT38I GRGCYC 2 cut(s) 1129, 1495
EgeI GGCGCC 1 cut(s) 561
EheI GGCGCC 1 cut(s) 561
FaeI CATG 7 cut(s) 130, 154, 260, 991, 1051, 1384, 1403
FatI CATG 7 cut(s) 126, 150, 256, 987, 1047, 1380, 1399
FauI CCCGC 1 cut(s) 696
FauNDI CATATG 1 cut(s) 1314
FbaI TGATCA 2 cut(s) 253, 1396
Fnu4HI GCNGC 4 cut(s) 66, 641, 833, 1091
FokI GGATG 3 cut(s) 630, 1332, 1435
FriOI GRGCYC 2 cut(s) 1129, 1495
Fsp4HI GCNGC 4 cut(s) 66, 641, 833, 1091
FspBI CTAG 1 cut(s) 705
GlaI GCGC 2 cut(s) 155, 561
GluI GCNGC 4 cut(s) 66, 641, 833, 1091
GsaI CCCAGC 1 cut(s) 1158
GsuI CTGGAG 2 cut(s) 951, 1251
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 3 cut(s) 75, 1159, 1437
HapII CCGG 1 cut(s) 539
HhaI GCGC 2 cut(s) 156, 562
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 7 cut(s) 130, 154, 260, 991, 1051, 1384, 1403
Hin6I GCGC 2 cut(s) 154, 560
HinP1I GCGC 2 cut(s) 154, 560
HincII GTYRAC 3 cut(s) 887, 917, 947
HindII GTYRAC 3 cut(s) 887, 917, 947
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 7 cut(s) 86, 269, 355, 652, 973, 1285, 1324
HpaII CCGG 1 cut(s) 539
HphI GGTGA 7 cut(s) 39, 239, 432, 1148, 1154, 1387, 1406
Hpy166II GTNNAC 7 cut(s) 57, 176, 865, 887, 917, 947, 1378
Hpy188III TCNNGA 6 cut(s) 251, 287, 322, 328, 968, 1417
Hpy8I GTNNAC 7 cut(s) 57, 176, 865, 887, 917, 947, 1378
HpyAV CCTTC 2 cut(s) 158, 1202
HpyCH4III ACNGT 4 cut(s) 115, 189, 759, 1250
HpyCH4V TGCA 8 cut(s) 301, 386, 697, 808, 832, 997, 1169, 1406
HpyF10VI GCNNNNNNNGC 3 cut(s) 829, 1090, 1124
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 7 cut(s) 130, 154, 260, 991, 1051, 1384, 1403
HspAI GCGC 2 cut(s) 154, 560
KasI GGCGCC 1 cut(s) 559
Ksp22I TGATCA 2 cut(s) 253, 1396
Kzo9I GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
LmnI GCTCC 4 cut(s) 1067, 1124, 1309, 1498
Lsp1109I GCAGC 4 cut(s) 52, 652, 844, 1102
LweI GCATC 3 cut(s) 222, 652, 1033
MabI ACCWGGT 2 cut(s) 910, 1024
MaeI CTAG 1 cut(s) 705
MaeIII GTNAC 4 cut(s) 183, 574, 792, 1340
MalI GATC 7 cut(s) 46, 255, 326, 397, 672, 1109, 1398
MboI GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
MboII GAAGA 4 cut(s) 95, 390, 393, 475
MflI RGATCY 3 cut(s) 324, 395, 670
MhlI GDGCHC 2 cut(s) 1129, 1495
MlsI TGGCCA 2 cut(s) 75, 1437
MluCI AATT 7 cut(s) 105, 168, 194, 365, 494, 779, 1331
MluNI TGGCCA 2 cut(s) 75, 1437
Mly113I GGCGCC 1 cut(s) 560
MlyI GAGTC 5 cut(s) 80, 349, 661, 967, 1279
MmeI TCCRAC 2 cut(s) 171, 778
Mox20I TGGCCA 2 cut(s) 75, 1437
MroXI GAANNNNTTC 1 cut(s) 172
MscI TGGCCA 2 cut(s) 75, 1437
MseI TTAA 2 cut(s) 245, 1452
MslI CAYNNNNRTG 1 cut(s) 992
Msp20I TGGCCA 2 cut(s) 75, 1437
MspI CCGG 1 cut(s) 539
MspR9I CCNGG 5 cut(s) 17, 882, 912, 1026, 1179
MvaI CCWGG 5 cut(s) 17, 882, 912, 1026, 1179
MwoI GCNNNNNNNGC 3 cut(s) 829, 1090, 1124
NarI GGCGCC 1 cut(s) 560
NdeI CATATG 1 cut(s) 1314
NdeII GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
NlaIII CATG 7 cut(s) 130, 154, 260, 991, 1051, 1384, 1403
NlaIV GGNNCC 6 cut(s) 546, 561, 672, 714, 1239, 1494
NmuCI GTSAC 2 cut(s) 183, 574
NspI RCATGY 1 cut(s) 991
PaeI GCATGC 1 cut(s) 991
PaqCI CACCTGC 1 cut(s) 793
PasI CCCWGGG 1 cut(s) 1178
PdmI GAANNNNTTC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 269, 1324
PflMI CCANNNNNTGG 1 cut(s) 1423
PkrI GCNGC 4 cut(s) 67, 642, 834, 1092
PleI GAGTC 5 cut(s) 80, 349, 660, 967, 1279
PluTI GGCGCC 1 cut(s) 563
PpsI GAGTC 5 cut(s) 80, 349, 660, 967, 1279
Psp124BI GAGCTC 1 cut(s) 1129
Psp6I CCWGG 5 cut(s) 15, 880, 910, 1024, 1177
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 5 cut(s) 15, 880, 910, 1024, 1177
PspN4I GGNNCC 6 cut(s) 546, 561, 672, 714, 1239, 1494
PspPI GGNCC 1 cut(s) 1157
PsrI GAACNNNNNNTAC 4 cut(s) 887, 917, 919, 949
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 3 cut(s) 324, 395, 670
RsaI GTAC 4 cut(s) 56, 866, 1186, 1479
RsaNI GTAC 4 cut(s) 55, 865, 1185, 1478
RseI CAYNNNNRTG 1 cut(s) 992
SacI GAGCTC 1 cut(s) 1129
SaqAI TTAA 2 cut(s) 245, 1452
SatI GCNGC 4 cut(s) 66, 641, 833, 1091
Sau3AI GATC 7 cut(s) 44, 253, 324, 395, 670, 1107, 1396
Sau96I GGNCC 1 cut(s) 1157
SchI GAGTC 5 cut(s) 80, 349, 661, 967, 1279
ScrFI CCNGG 5 cut(s) 17, 882, 912, 1026, 1179
SduI GDGCHC 2 cut(s) 1129, 1495
SexAI ACCWGGT 2 cut(s) 910, 1024
SfaNI GCATC 3 cut(s) 222, 652, 1033
SfoI GGCGCC 1 cut(s) 561
SmiI ATTTAAAT 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 992
SmlI CTYRAG 3 cut(s) 278, 287, 732
SmoI CTYRAG 3 cut(s) 278, 287, 732
SphI GCATGC 1 cut(s) 991
Sse9I AATT 7 cut(s) 105, 168, 194, 365, 494, 779, 1331
SsiI CCGC 3 cut(s) 689, 1065, 1328
SspDI GGCGCC 1 cut(s) 559
SspI AATATT 1 cut(s) 659
SspMI CTAG 1 cut(s) 705
SstI GAGCTC 1 cut(s) 1129
StyD4I CCNGG 5 cut(s) 15, 880, 910, 1024, 1177
SwaI ATTTAAAT 1 cut(s) 246
TaaI ACNGT 4 cut(s) 115, 189, 759, 1250
TaqI TCGA 2 cut(s) 682, 1446
TasI AATT 7 cut(s) 105, 168, 194, 365, 494, 779, 1331
TatI WGTACW 3 cut(s) 54, 1184, 1477
TfiI GAWTC 2 cut(s) 269, 1324
Tru1I TTAA 2 cut(s) 245, 1452
Tru9I TTAA 2 cut(s) 245, 1452
TseFI GTSAC 2 cut(s) 183, 574
TseI GCWGC 4 cut(s) 65, 640, 832, 1090
Tsp45I GTSAC 2 cut(s) 183, 574
TspDTI ATGAA 4 cut(s) 143, 393, 1324, 1473
Van91I CCANNNNNTGG 1 cut(s) 1423
XagI CCTNNNNNAGG 2 cut(s) 14, 1509
XceI RCATGY 1 cut(s) 991
XmnI GAANNNNTTC 1 cut(s) 172
XspI CTAG 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.