AT4G28770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011210
Physical Location & Seq
Reverse (-)
14211855 .. 14213969
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28770.1

Sequence Viewer

Length: 846 bp
ATGCGACACAATTGTTGCCATGTCTCGTTCGCTTCGATTCTCAAGATCCTTAATTTTCTCCAAGCTTTCATCGGTATATCAATCATAATCTACTCGATTTGGATGCTCGATCAATACAATCATCACGTTCCCGTTGACCCTCCTCCGTCTCAGCCTCCGGCAGCTTCATCACCGGATTCTTCTTATTCCTTCTCTAATTCAATAATTGAGATCAACAGTGTTTCTGATTCTTTGAAGAATCCGATCGATTTCGTATCGGGTATCGTTCTCGGGTCTGGTGGTGGAGATTCGGGTTTCAATCTCCGTTCTTTAGATCTTCCTGCTCCATGGTTTATCTACTGTTTCATGGCGATTGGGATTTTGGTCTGCATTGTCACTATCATTGGTTTCATTGCAGCTGAAGCTATCAATGGATGTTGCTTGTGTTTCTATTCAATTCTCAAAACTCTTTTAATCATTATCGAAGCAGCTCTAGTGGGATTCATAGTGATTGACCGTCACTGGGAGAAGGATCTTCCTTATGATCCAACTGGAGAACTCAATAGTCTTCGAGCTTTCATCGAAGAAAACATCGATATTTGCAAATGGGTCGGAATTGTTGTGGTAGCCATCCAGTTACTGTCATTGCTACTGGCTTTGGTTTTGAGAGCTATGGTTTCACCTCGGCAATCAGAGCTTGATGATGAAGATGATTTTGAGAATCCGATGAGTAGAGCACGAGACAATCTTCTTGGTCCACAGGCAAACCAGACATCTTCTGGATCAAGCAATATTGACAACTGGAGGTCCCGAATCAGAGAGAAGTACGGATTGATCAACAGTCAGAGCCACACTCCATCAGCTTAA

Protein Analysis

281

Amino Acids

30.98

Weight (kDa)

4.86

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 13 - 168 1.9e-07 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20230 AT4G28770 AT4G28770
fragaria_vesca FvH4_2g19890 FvH4_2g19890
malus_domestica MD05G1183500.v1.1 MD10G1171300.v1.1
prunus_persica Prupe.8G209700_v2.0.a1
pyrus_communis pycom05g16850 pycom10g14770
rosa_chinensis RchiOBHm_Chr6g0285581
rosa_laevigata RLG00000012625
rosa_multiflora Rmu_sc0012495.1_g000001
rosa_roxburghii Rroxscaffold_7G00182930
rosa_rugosa Rorug06G0175800
rosa_samantha Rh6AG287300 Rh6BG288500 Rh6CG289700 Rh6DG282500
rosa_wichuraiana Rw6G024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 40, 518, 519, 769
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 1 cut(s) 502
AgsI TTSAA 4 cut(s) 201, 235, 298, 435
AluBI AGCT 9 cut(s) 65, 164, 398, 404, 470, 554, 650, 676, 842
AluI AGCT 9 cut(s) 65, 164, 398, 404, 470, 554, 650, 676, 842
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 3 cut(s) 28, 153, 714
AlwI GGATC 4 cut(s) 40, 518, 519, 769
AlwNI CAGNNNCTG 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 269
ApeKI GCWGC 3 cut(s) 161, 395, 467
AspS9I GGNCC 2 cut(s) 734, 786
AsuHPI GGTGA 2 cut(s) 162, 651
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 2 cut(s) 734, 786
BauI CACGAG 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 3 cut(s) 173, 407, 479
BccI CCATC 2 cut(s) 617, 844
BcgI CGANNNNNNTGC 4 cut(s) 85, 119, 571, 605
BclI TGATCA 1 cut(s) 813
BcoDI GTCTC 3 cut(s) 28, 153, 714
BfaI CTAG 1 cut(s) 473
BglII AGATCT 1 cut(s) 313
BisI GCNGC 3 cut(s) 162, 396, 468
BlsI GCNGC 3 cut(s) 163, 397, 469
Bme18I GGWCC 2 cut(s) 734, 786
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 734, 786
BmiI GGNNCC 1 cut(s) 788
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 539
BplI GAGNNNNNCTC 1 cut(s) 817
BpmI CTGGAG 2 cut(s) 552, 802
BpuEI CTTGAG 1 cut(s) 26
Bsa29I ATCGAT 2 cut(s) 246, 573
BsaJI CCNNGG 2 cut(s) 326, 662
BsaWI WCCGGW 1 cut(s) 172
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse1I ACTGG 5 cut(s) 506, 535, 613, 636, 785
Bse3DI GCAATG 2 cut(s) 390, 623
BseCI ATCGAT 2 cut(s) 246, 573
BseDI CCNNGG 2 cut(s) 326, 662
BseGI GGATG 3 cut(s) 108, 419, 609
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMI GCAATG 2 cut(s) 390, 623
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 5 cut(s) 506, 535, 613, 636, 785
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 3 cut(s) 173, 407, 479
Bsh1285I CGRYCG 1 cut(s) 246
BshVI ATCGAT 2 cut(s) 246, 573
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 718
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 2 cut(s) 158, 173
BslFI GGGAC 1 cut(s) 772
BslI CCNNNNNNNGG 1 cut(s) 502
BsmAI GTCTC 3 cut(s) 28, 153, 714
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 772
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
Bsp19I CCATGG 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 163
BspDI ATCGAT 2 cut(s) 246, 573
BspLI GGNNCC 1 cut(s) 788
BspPI GGATC 4 cut(s) 40, 518, 519, 769
BsrDI GCAATG 2 cut(s) 390, 623
BsrI ACTGG 5 cut(s) 506, 535, 613, 636, 785
BssECI CCNNGG 2 cut(s) 326, 662
BssMI GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 326
Bst2BI CACGAG 1 cut(s) 717
Bst4CI ACNGT 5 cut(s) 218, 341, 497, 621, 821
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 326
BstF5I GGATG 3 cut(s) 108, 419, 609
BstKTI GATC 9 cut(s) 48, 112, 213, 246, 316, 514, 526, 764, 816
BstMAI GTCTC 3 cut(s) 28, 153, 714
BstMBI GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 401, 673
BstV1I GCAGC 3 cut(s) 173, 407, 479
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 3 cut(s) 45, 313, 511
BstYI RGATCY 3 cut(s) 45, 313, 511
Bsu15I ATCGAT 2 cut(s) 246, 573
BsuTUI ATCGAT 2 cut(s) 246, 573
BtgI CCRYGG 1 cut(s) 326
BtsCI GGATG 3 cut(s) 108, 419, 609
BtsIMutI CAGTG 2 cut(s) 223, 499
CaiI CAGNNNCTG 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 734, 786
ClaI ATCGAT 2 cut(s) 246, 573
Csp6I GTAC 1 cut(s) 805
CviAII CATG 3 cut(s) 20, 327, 346
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 1 cut(s) 150
DpnI GATC 9 cut(s) 47, 111, 212, 245, 315, 513, 525, 763, 815
DpnII GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
Eco130I CCWWGG 1 cut(s) 326
Eco47I GGWCC 2 cut(s) 734, 786
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 786
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 3 cut(s) 23, 330, 349
FaiI YATR 8 cut(s) 21, 77, 86, 328, 347, 485, 522, 653
FaqI GGGAC 1 cut(s) 772
FatI CATG 3 cut(s) 19, 326, 345
FbaI TGATCA 1 cut(s) 813
Fnu4HI GCNGC 3 cut(s) 162, 396, 468
FokI GGATG 3 cut(s) 115, 426, 596
Fsp4HI GCNGC 3 cut(s) 162, 396, 468
FspBI CTAG 1 cut(s) 473
GluI GCNGC 3 cut(s) 162, 396, 468
GsuI CTGGAG 2 cut(s) 552, 802
HapII CCGG 2 cut(s) 158, 173
Hin1II CATG 3 cut(s) 23, 330, 349
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 8 cut(s) 37, 176, 227, 238, 287, 480, 700, 792
HpaII CCGG 2 cut(s) 158, 173
HphI GGTGA 2 cut(s) 162, 651
Hpy166II GTNNAC 2 cut(s) 136, 737
Hpy188I TCNGA 7 cut(s) 226, 243, 593, 673, 705, 797, 825
Hpy188III TCNNGA 3 cut(s) 43, 759, 789
Hpy8I GTNNAC 2 cut(s) 136, 737
HpyAV CCTTC 2 cut(s) 199, 502
HpyCH4III ACNGT 5 cut(s) 218, 341, 497, 621, 821
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 3 cut(s) 369, 395, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 673
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 3 cut(s) 23, 330, 349
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
LmnI GCTCC 1 cut(s) 328
Lsp1109I GCAGC 3 cut(s) 173, 407, 479
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 3 cut(s) 373, 497, 615
MalI GATC 9 cut(s) 47, 111, 212, 245, 315, 513, 525, 763, 815
MboI GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
MboII GAAGA 9 cut(s) 171, 247, 308, 506, 539, 575, 698, 719, 747
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 3 cut(s) 45, 313, 511
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 6 cut(s) 10, 52, 196, 204, 435, 594
MmeI TCCRAC 2 cut(s) 551, 571
MnlI CCTC 5 cut(s) 150, 153, 165, 672, 777
MseI TTAA 3 cut(s) 51, 452, 844
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 2 cut(s) 158, 173
MunI CAATTG 1 cut(s) 10
MwoI GCNNNNNNNGC 2 cut(s) 401, 673
NcoI CCATGG 1 cut(s) 326
NdeII GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
NlaIII CATG 3 cut(s) 23, 330, 349
NlaIV GGNNCC 1 cut(s) 788
NmeAIII GCCGAG 1 cut(s) 643
NmuCI GTSAC 2 cut(s) 373, 497
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PfeI GAWTC 8 cut(s) 37, 176, 227, 238, 287, 480, 700, 792
PkrI GCNGC 3 cut(s) 163, 397, 469
Ple19I CGATCG 1 cut(s) 246
PpuMI RGGWCCY 1 cut(s) 786
Psp5II RGGWCCY 1 cut(s) 786
PspN4I GGNNCC 1 cut(s) 788
PspPI GGNCC 2 cut(s) 734, 786
PspPPI RGGWCCY 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 619
PsuI RGATCY 3 cut(s) 45, 313, 511
PvuI CGATCG 1 cut(s) 246
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
SaqAI TTAA 3 cut(s) 51, 452, 844
SatI GCNGC 3 cut(s) 162, 396, 468
Sau3AI GATC 9 cut(s) 45, 109, 210, 243, 313, 511, 523, 761, 813
Sau96I GGNCC 2 cut(s) 734, 786
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 1 cut(s) 93
SinI GGWCC 2 cut(s) 734, 786
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 6 cut(s) 10, 52, 196, 204, 435, 594
SspI AATATT 1 cut(s) 772
SspMI CTAG 1 cut(s) 473
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 5 cut(s) 218, 341, 497, 621, 821
TaiI ACGT 1 cut(s) 129
TaqI TCGA 8 cut(s) 35, 95, 108, 246, 462, 550, 561, 573
TasI AATT 6 cut(s) 10, 52, 196, 204, 435, 594
TfiI GAWTC 8 cut(s) 37, 176, 227, 238, 287, 480, 700, 792
Tru1I TTAA 3 cut(s) 51, 452, 844
Tru9I TTAA 3 cut(s) 51, 452, 844
TscAI CASTG 2 cut(s) 223, 506
TseFI GTSAC 2 cut(s) 373, 497
TseI GCWGC 3 cut(s) 161, 395, 467
Tsp45I GTSAC 2 cut(s) 373, 497
TspDTI ATGAA 7 cut(s) 58, 156, 334, 379, 472, 547, 699
TspGWI ACGGA 3 cut(s) 135, 293, 822
TspRI CASTG 2 cut(s) 223, 506
VpaK11BI GGWCC 2 cut(s) 734, 786
XcmI CCANNNNNNNNNTGG 1 cut(s) 755
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.