FvH4_2g19890

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011210
Physical Location & Seq
Forward (+)
16799562 .. 16803164
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19890.t2

Sequence Viewer

Length: 864 bp
ATGCGGCGGAATTGCTGCCATGTGTCCCTAGCCTTCGTGCTCAAATTCCTCAACTTCCTTCAAGCCTTCGTTGGCGTCTCGATCTTACTCTACTCGGCGTGGATGCTTGATCAGTGGAACCATCACATTCCGGTGTTCCCTCCGTCCTCCGCGCCTTCCCCGGACCCTCCTTTCTCACTCTCGCTGAGCTCCGGCGCCGGCGCAGTTTCCGATCAGGTCAATTTTGGAGTCCAGTTGGTGTCTGGGTTGGATGAAGGCATGGGGTTTCAATTGAATGCCCTGAACCTCCCTGCTCCTTGGTTCATATACTCTTTTATGGGAGTGGGGGCTTTGCTCCTCTGCATTACTTTCATAGGTTGTATCGCAGCTGAATCGATCAACCGTTGCTGCTTGTGTTTCTACAGTCTACTCATAACTGTACTAGCTCTAGCAGAAGCAGCTCTGGTAGCTTTCATAGCAATTGATCACCATTGGGACAAGGATCTTCCAGTTGACCCAACTGGTGAACTTGAGAGTTTTCAATCTTTCATTGAAAAAAACATTGACATATGTAGGTGGGTTGGGATAGCAGTTGTTGGAATACAGGCTTTATCTCTGCTACTAGCAATAATTCTACGAGGCATGATATCTACCCATAAAGCTGACTATGATGTTGAAGATGAGTATGATGCTAGGGGTAGAACTCAGGAACCACTGCTAAATCAACATTTAAGCCAAGCATCTGGATCAACTAAGGTGGATGGCAGAGGGACTCACTCTGACATTTGGAGCTCACGTATAAGAGATAAGTATGGACTGAGCTCTGGTGATAAATACAATTCCGTAAATCAGAATGCATCATTGAGTATGAAATCCAGGCAGTGA

Protein Analysis

288

Amino Acids

31.49

Weight (kDa)

5.55

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 158 2.8e-08 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20230 AT4G28770 AT4G28770
fragaria_vesca FvH4_2g19890 FvH4_2g19890
malus_domestica MD05G1183500.v1.1 MD10G1171300.v1.1
prunus_persica Prupe.8G209700_v2.0.a1
pyrus_communis pycom05g16850 pycom10g14770
rosa_chinensis RchiOBHm_Chr6g0285581
rosa_laevigata RLG00000012625
rosa_multiflora Rmu_sc0012495.1_g000001
rosa_roxburghii Rroxscaffold_7G00182930
rosa_rugosa Rorug06G0175800
rosa_samantha Rh6AG287300 Rh6BG288500 Rh6CG289700 Rh6DG282500
rosa_wichuraiana Rw6G024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 406
AccII CGCG 1 cut(s) 152
AciI CCGC 3 cut(s) 4, 7, 150
AclWI GGATC 2 cut(s) 489, 733
AcsI RAATTY 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 75, 195
AfaI GTAC 1 cut(s) 420
AgsI TTSAA 6 cut(s) 62, 269, 274, 521, 533, 656
AjnI CCWGG 1 cut(s) 854
AluBI AGCT 8 cut(s) 189, 368, 425, 440, 449, 641, 771, 801
AluI AGCT 8 cut(s) 189, 368, 425, 440, 449, 641, 771, 801
Alw21I GWGCWC 4 cut(s) 42, 191, 773, 803
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 2 cut(s) 489, 733
ApeKI GCWGC 4 cut(s) 15, 365, 387, 437
ApoI RAATTY 1 cut(s) 44
AspLEI GCGC 3 cut(s) 154, 197, 203
AspS9I GGNCC 1 cut(s) 163
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 3 cut(s) 458, 515, 818
AvaII GGWCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 3 cut(s) 191, 773, 803
Bbv12I GWGCWC 4 cut(s) 42, 191, 773, 803
BbvI GCAGC 4 cut(s) 2, 374, 377, 449
BccI CCATC 2 cut(s) 129, 734
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BciT130I CCWGG 1 cut(s) 856
BclI TGATCA 2 cut(s) 109, 463
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 5 cut(s) 29, 422, 428, 602, 672
BfmI CTRYAG 1 cut(s) 400
BfoI RGCGCY 1 cut(s) 198
BisI GCNGC 5 cut(s) 5, 16, 366, 388, 438
BlpI GCTNAGC 1 cut(s) 185
BlsI GCNGC 5 cut(s) 6, 17, 367, 389, 439
Bme1390I CCNGG 2 cut(s) 161, 856
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 4 cut(s) 119, 165, 196, 690
BmrFI CCNGG 2 cut(s) 161, 856
BmsI GCATC 4 cut(s) 93, 658, 728, 845
Bpu1102I GCTNAGC 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 161
Bsa29I ATCGAT 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 776
BsaHI GRCGYC 2 cut(s) 75, 195
BsaJI CCNNGG 2 cut(s) 159, 296
BsaWI WCCGGW 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 760, 790
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 3 cut(s) 232, 488, 505
BseBI CCWGG 1 cut(s) 856
BseCI ATCGAT 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 159, 296
BseGI GGATG 3 cut(s) 108, 256, 745
BseMII CTCAG 3 cut(s) 176, 698, 788
BseNI ACTGG 3 cut(s) 232, 488, 505
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 4 cut(s) 2, 374, 377, 449
Bsh1236I CGCG 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 374
BsiHKAI GWGCWC 4 cut(s) 42, 191, 773, 803
BsiSI CCGG 4 cut(s) 131, 161, 192, 198
BslFI GGGAC 3 cut(s) 10, 488, 763
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 3 cut(s) 10, 488, 763
BsmI GAATGC 2 cut(s) 280, 838
Bsp1286I GDGCHC 4 cut(s) 42, 191, 773, 803
Bsp143I GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
Bsp1720I GCTNAGC 1 cut(s) 185
BspACI CCGC 3 cut(s) 4, 7, 150
BspCNI CTCAG 3 cut(s) 177, 697, 789
BspDI ATCGAT 1 cut(s) 374
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 4 cut(s) 119, 165, 196, 690
BspPI GGATC 2 cut(s) 489, 733
BspT107I GGYRCC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 3 cut(s) 232, 488, 505
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 159, 296
BssMI GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
BssNI GRCGYC 2 cut(s) 75, 195
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 856
Bst4CI ACNGT 3 cut(s) 383, 404, 418
BstACI GRCGYC 2 cut(s) 75, 195
BstBAI YACGTR 1 cut(s) 776
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 4 cut(s) 185, 684, 732, 797
BstF5I GGATG 3 cut(s) 108, 256, 745
BstFNI CGCG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 3 cut(s) 154, 197, 203
BstKTI GATC 7 cut(s) 84, 112, 214, 378, 466, 484, 728
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
BstMWI GCNNNNNNNGC 3 cut(s) 437, 446, 455
BstNI CCWGG 1 cut(s) 856
BstSCI CCNGG 2 cut(s) 159, 854
BstSFI CTRYAG 1 cut(s) 400
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 4 cut(s) 2, 374, 377, 449
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 1 cut(s) 481
Bsu15I ATCGAT 1 cut(s) 374
BsuTUI ATCGAT 1 cut(s) 374
BtsCI GGATG 3 cut(s) 108, 256, 745
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 2 cut(s) 119, 692
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 3 cut(s) 154, 197, 203
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 374
CseI GACGC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 419
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 3 cut(s) 20, 259, 622
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 4 cut(s) 185, 684, 732, 797
DinI GGCGCC 1 cut(s) 196
DpnI GATC 7 cut(s) 83, 111, 213, 377, 465, 483, 727
DpnII GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
EciI GGCGGA 1 cut(s) 22
Ecl136II GAGCTC 3 cut(s) 189, 771, 801
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 3 cut(s) 191, 773, 803
Eco32I GATATC 1 cut(s) 627
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 3 cut(s) 189, 771, 801
EcoICRI GAGCTC 3 cut(s) 189, 771, 801
EcoRII CCWGG 1 cut(s) 854
EcoRV GATATC 1 cut(s) 627
EcoT14I CCWWGG 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 838
EcoT38I GRGCYC 3 cut(s) 191, 773, 803
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 296
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 23, 262, 625
FaqI GGGAC 3 cut(s) 10, 488, 763
FatI CATG 3 cut(s) 19, 258, 621
FauNDI CATATG 1 cut(s) 548
FbaI TGATCA 2 cut(s) 109, 463
FblI GTMKAC 1 cut(s) 406
Fnu4HI GCNGC 5 cut(s) 5, 16, 366, 388, 438
FokI GGATG 3 cut(s) 115, 263, 752
FriOI GRGCYC 3 cut(s) 191, 773, 803
Fsp4HI GCNGC 5 cut(s) 5, 16, 366, 388, 438
FspBI CTAG 5 cut(s) 29, 422, 428, 602, 672
GlaI GCGC 3 cut(s) 153, 196, 202
GluI GCNGC 5 cut(s) 5, 16, 366, 388, 438
HaeII RGCGCY 1 cut(s) 198
HapII CCGG 4 cut(s) 131, 161, 192, 198
HgaI GACGC 1 cut(s) 64
HhaI GCGC 3 cut(s) 154, 197, 203
Hin1I GRCGYC 2 cut(s) 75, 195
Hin1II CATG 3 cut(s) 23, 262, 625
Hin6I GCGC 3 cut(s) 152, 195, 201
HinP1I GCGC 3 cut(s) 152, 195, 201
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 3 cut(s) 228, 371, 751
HpaII CCGG 4 cut(s) 131, 161, 192, 198
HphI GGTGA 3 cut(s) 458, 515, 818
Hpy166II GTNNAC 3 cut(s) 407, 493, 506
Hpy188I TCNGA 3 cut(s) 211, 760, 831
Hpy188III TCNNGA 3 cut(s) 79, 686, 723
Hpy8I GTNNAC 3 cut(s) 407, 493, 506
HpyAV CCTTC 5 cut(s) 43, 68, 76, 165, 248
HpyCH4III ACNGT 3 cut(s) 383, 404, 418
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 2 cut(s) 342, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 446, 455
HpyF3I CTNAG 4 cut(s) 185, 684, 732, 797
HpySE526I ACGT 1 cut(s) 775
Hsp92I GRCGYC 2 cut(s) 75, 195
Hsp92II CATG 3 cut(s) 23, 262, 625
HspAI GCGC 3 cut(s) 152, 195, 201
KasI GGCGCC 1 cut(s) 194
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 109, 463
Kzo9I GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
LmnI GCTCC 4 cut(s) 194, 298, 339, 768
Lsp1109I GCAGC 4 cut(s) 2, 374, 377, 449
LweI GCATC 4 cut(s) 93, 658, 728, 845
MaeI CTAG 5 cut(s) 29, 422, 428, 602, 672
MaeII ACGT 1 cut(s) 775
MalI GATC 7 cut(s) 83, 111, 213, 377, 465, 483, 727
MboI GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
MboII GAAGA 2 cut(s) 476, 668
MfeI CAATTG 2 cut(s) 269, 459
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 4 cut(s) 42, 191, 773, 803
MluCI AATT 7 cut(s) 10, 44, 220, 269, 459, 609, 817
Mly113I GGCGCC 1 cut(s) 195
MlyI GAGTC 2 cut(s) 237, 745
MmeI TCCRAC 2 cut(s) 228, 556
MnlI CCTC 8 cut(s) 59, 150, 157, 177, 296, 347, 611, 740
Mph1103I ATGCAT 1 cut(s) 838
MreI CGCCGGCG 1 cut(s) 197
MroNI GCCGGC 1 cut(s) 197
MseI TTAA 1 cut(s) 710
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 4 cut(s) 131, 161, 192, 198
MspR9I CCNGG 2 cut(s) 161, 856
MunI CAATTG 2 cut(s) 269, 459
Mva1269I GAATGC 2 cut(s) 280, 838
MvaI CCWGG 1 cut(s) 856
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 3 cut(s) 437, 446, 455
NaeI GCCGGC 1 cut(s) 199
NarI GGCGCC 1 cut(s) 195
NciI CCSGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 548
NdeII GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 3 cut(s) 23, 262, 625
NlaIV GGNNCC 4 cut(s) 119, 165, 196, 690
NmeAIII GCCGAG 1 cut(s) 74
NsiI ATGCAT 1 cut(s) 838
PctI GAATGC 2 cut(s) 280, 838
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 5 cut(s) 6, 17, 367, 389, 439
PleI GAGTC 2 cut(s) 236, 745
PluTI GGCGCC 1 cut(s) 198
PpsI GAGTC 2 cut(s) 236, 745
Ppu21I YACGTR 1 cut(s) 776
Psp124BI GAGCTC 3 cut(s) 191, 773, 803
Psp6I CCWGG 1 cut(s) 854
PspGI CCWGG 1 cut(s) 854
PspN4I GGNNCC 4 cut(s) 119, 165, 196, 690
PspPI GGNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 3 cut(s) 191, 773, 803
SaqAI TTAA 1 cut(s) 710
SatI GCNGC 5 cut(s) 5, 16, 366, 388, 438
Sau3AI GATC 7 cut(s) 81, 109, 211, 375, 463, 481, 725
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 2 cut(s) 237, 745
ScrFI CCNGG 2 cut(s) 161, 856
SduI GDGCHC 4 cut(s) 42, 191, 773, 803
SfaNI GCATC 4 cut(s) 93, 658, 728, 845
SfcI CTRYAG 1 cut(s) 400
SfoI GGCGCC 1 cut(s) 196
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 7 cut(s) 10, 44, 220, 269, 459, 609, 817
SsiI CCGC 3 cut(s) 4, 7, 150
SspDI GGCGCC 1 cut(s) 194
SspMI CTAG 5 cut(s) 29, 422, 428, 602, 672
SstI GAGCTC 3 cut(s) 191, 773, 803
StyD4I CCNGG 2 cut(s) 159, 854
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 3 cut(s) 383, 404, 418
TaiI ACGT 1 cut(s) 778
TaqI TCGA 2 cut(s) 80, 374
TasI AATT 7 cut(s) 10, 44, 220, 269, 459, 609, 817
TatI WGTACW 1 cut(s) 418
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 710
Tru9I TTAA 1 cut(s) 710
TscAI CASTG 2 cut(s) 119, 699
TseI GCWGC 4 cut(s) 15, 365, 387, 437
TspDTI ATGAA 6 cut(s) 267, 292, 340, 442, 517, 863
TspGWI ACGGA 2 cut(s) 132, 811
TspRI CASTG 2 cut(s) 119, 699
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 406
XspI CTAG 5 cut(s) 29, 422, 428, 602, 672
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.