MD05G1183500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011210
Physical Location & Seq
Forward (+)
31199287 .. 31202068
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1183500.v1.1.491

Sequence Viewer

Length: 864 bp
ATGCGGCCCAATTGCTGCCATGTATCCCTAGCTTTCGTCCTCAAACTCCTCAACTTTCTTCAAGCTTTCATCGGAGTTTCGATCTTAGTCTACTCGGCATGGATGCTCAACCAGTGGAACCACCACATTCCGATAGTCCCGCCACCGCCTTCAGCTCCTTCTCCTGATTCCTCGTCCTTCTCCTCCCTCTTATTGAGCTCCGAGTCGGCTGCGGATGCCGATCAGATCACGCCGTTGGATTTTGCTGTCCATTTGGTTTCTGGGTTCGATGAGGGATTGGGGTTCGGATTGAACTCGTTGAAGCTTCCCGCGCCCTGGTTCATCTACTCTTTTATGGGACTGGGGATTTTGCTCTGTTGCGTTACTTTCATAGGTTGTATTGCAGCCGCAGCAATTAATGGCTGCTGCCTTTGCTTTTATACAATACTTGTAACCATGCTCACTCTACTAGAAGCAGCTTTGGTGGCATTCATTTTAATTGATCGCCGTTGGGAAAAGGATCTTCCATTTGACCCAACTGGTCAACTTGACAGTTTCCGATCTTTTGTTGAAGAGAATATTGACATCTGTAGGTGGGTTGGGCTCGGGGTCATTGTAATACAGGTTTTATCTCTGCTACTATCAATAACTCTGAGAGGCATGCTTTCTACTCACAAACCTGACTATGATGTTGAAGATGATTATGATGCTAGGGCTAGAACTCGGGAGCCCCTTTTAAAACAACAATTAAACCAAGCATCTGGATCAACCAAGGGTGATGGGAAAGGGACTCACTCTGACATTTGGAGCTCACGGATAAGAGATAAGTATGGATTGAGCTCTCTGAATCAGAATGCGTCACCAAGTATGCAATCCAAGCAGTAA

Protein Analysis

288

Amino Acids

31.58

Weight (kDa)

5.46

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 164 6.9e-06 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20230 AT4G28770 AT4G28770
fragaria_vesca FvH4_2g19890 FvH4_2g19890
malus_domestica MD05G1183500.v1.1 MD10G1171300.v1.1
prunus_persica Prupe.8G209700_v2.0.a1
pyrus_communis pycom05g16850 pycom10g14770
rosa_chinensis RchiOBHm_Chr6g0285581
rosa_laevigata RLG00000012625
rosa_multiflora Rmu_sc0012495.1_g000001
rosa_roxburghii Rroxscaffold_7G00182930
rosa_rugosa Rorug06G0175800
rosa_samantha Rh6AG287300 Rh6BG288500 Rh6CG289700 Rh6DG282500
rosa_wichuraiana Rw6G024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 311
AciI CCGC 6 cut(s) 4, 140, 146, 212, 309, 387
AclWI GGATC 2 cut(s) 507, 751
AcuI CTGAAG 1 cut(s) 135
AfiI CCNNNNNNNGG 1 cut(s) 315
AgsI TTSAA 5 cut(s) 62, 292, 301, 551, 674
AjnI CCWGG 1 cut(s) 314
AluBI AGCT 8 cut(s) 32, 65, 155, 198, 304, 458, 789, 819
AluI AGCT 8 cut(s) 32, 65, 155, 198, 304, 458, 789, 819
Alw21I GWGCWC 3 cut(s) 200, 791, 821
AlwI GGATC 2 cut(s) 507, 751
Ama87I CYCGRG 2 cut(s) 584, 702
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 7 cut(s) 15, 209, 383, 389, 402, 405, 455
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 767, 831
AvaI CYCGRG 2 cut(s) 584, 702
BanII GRGCYC 5 cut(s) 200, 585, 711, 791, 821
Bbv12I GWGCWC 3 cut(s) 200, 791, 821
BbvI GCAGC 7 cut(s) 2, 196, 389, 392, 395, 401, 467
BccI CCATC 1 cut(s) 752
BceAI ACGGC 2 cut(s) 217, 471
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 1 cut(s) 316
BciVI GTATCC 1 cut(s) 34
BfaI CTAG 4 cut(s) 29, 449, 690, 696
BfmI CTRYAG 1 cut(s) 568
BfuI GTATCC 1 cut(s) 34
BisI GCNGC 9 cut(s) 5, 16, 210, 384, 387, 390, 403, 406, 456
BlsI GCNGC 9 cut(s) 6, 17, 211, 385, 388, 391, 404, 407, 457
Bme1390I CCNGG 1 cut(s) 316
BmeT110I CYCGRG 2 cut(s) 584, 702
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 119, 708
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 4 cut(s) 93, 205, 676, 746
BmuI ACTGGG 1 cut(s) 350
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 314, 750
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse1I ACTGG 3 cut(s) 112, 345, 523
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 2 cut(s) 314, 750
BseGI GGATG 2 cut(s) 108, 220
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 3 cut(s) 112, 345, 523
BseRI GAGGAG 2 cut(s) 38, 172
BseXI GCAGC 7 cut(s) 2, 196, 389, 392, 395, 401, 467
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 7
BsiHKAI GWGCWC 3 cut(s) 200, 791, 821
BsiHKCI CYCGRG 2 cut(s) 584, 702
BslFI GGGAC 3 cut(s) 122, 351, 781
BslI CCNNNNNNNGG 1 cut(s) 315
BsmFI GGGAC 3 cut(s) 122, 351, 781
BsmI GAATGC 2 cut(s) 467, 838
BsnI GGCC 1 cut(s) 7
BsoBI CYCGRG 2 cut(s) 584, 702
Bsp1286I GDGCHC 5 cut(s) 200, 585, 711, 791, 821
Bsp143I GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
BspACI CCGC 6 cut(s) 4, 140, 146, 212, 309, 387
BspANI GGCC 1 cut(s) 7
BspCNI CTCAG 1 cut(s) 624
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 119, 708
BspPI GGATC 2 cut(s) 507, 751
BsrI ACTGG 3 cut(s) 112, 345, 523
BssECI CCNNGG 2 cut(s) 314, 750
BssMI GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
BssT1I CCWWGG 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 1 cut(s) 533
Bst6I CTCTTC 1 cut(s) 546
BstC8I GCNNGC 1 cut(s) 641
BstDEI CTNAG 2 cut(s) 85, 632
BstF5I GGATG 2 cut(s) 108, 220
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 7 cut(s) 84, 223, 228, 484, 502, 542, 746
BstMBI GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
BstMWI GCNNNNNNNGC 6 cut(s) 215, 310, 389, 411, 464, 856
BstNI CCWGG 1 cut(s) 316
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 314
BstSFI CTRYAG 1 cut(s) 568
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 7 cut(s) 2, 196, 389, 392, 395, 401, 467
BstX2I RGATCY 1 cut(s) 499
BstXI CCANNNNNNTGG 1 cut(s) 740
BstYI RGATCY 1 cut(s) 499
BsuI GTATCC 1 cut(s) 34
BsuRI GGCC 1 cut(s) 7
BtsCI GGATG 2 cut(s) 108, 220
BtsIMutI CAGTG 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 6
CseI GACGC 1 cut(s) 825
CviAII CATG 4 cut(s) 20, 99, 436, 640
DdeI CTNAG 2 cut(s) 85, 632
DpnI GATC 7 cut(s) 83, 222, 227, 483, 501, 541, 745
DpnII GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
DraI TTTAAA 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Ecl136II GAGCTC 3 cut(s) 198, 789, 819
Eco130I CCWWGG 1 cut(s) 750
Eco24I GRGCYC 5 cut(s) 200, 585, 711, 791, 821
Eco53kI GAGCTC 3 cut(s) 198, 789, 819
Eco57I CTGAAG 1 cut(s) 135
Eco88I CYCGRG 2 cut(s) 584, 702
EcoICRI GAGCTC 3 cut(s) 198, 789, 819
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 750
EcoT38I GRGCYC 5 cut(s) 200, 585, 711, 791, 821
ErhI CCWWGG 1 cut(s) 750
FaeI CATG 4 cut(s) 23, 102, 439, 643
FaqI GGGAC 3 cut(s) 122, 351, 781
FatI CATG 4 cut(s) 19, 98, 435, 639
FauI CCCGC 2 cut(s) 147, 316
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 9 cut(s) 5, 16, 210, 384, 387, 390, 403, 406, 456
FokI GGATG 2 cut(s) 115, 227
FriOI GRGCYC 5 cut(s) 200, 585, 711, 791, 821
Fsp4HI GCNGC 9 cut(s) 5, 16, 210, 384, 387, 390, 403, 406, 456
FspBI CTAG 4 cut(s) 29, 449, 690, 696
GlaI GCGC 1 cut(s) 312
GluI GCNGC 9 cut(s) 5, 16, 210, 384, 387, 390, 403, 406, 456
HaeIII GGCC 1 cut(s) 7
HgaI GACGC 1 cut(s) 825
HhaI GCGC 1 cut(s) 313
Hin1II CATG 4 cut(s) 23, 102, 439, 643
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HindIII AAGCTT 2 cut(s) 63, 302
HinfI GANTC 4 cut(s) 167, 203, 769, 826
HphI GGTGA 2 cut(s) 767, 831
Hpy166II GTNNAC 2 cut(s) 91, 524
Hpy188III TCNNGA 3 cut(s) 164, 704, 741
Hpy8I GTNNAC 2 cut(s) 91, 524
HpyAV CCTTC 3 cut(s) 159, 168, 187
HpyCH4III ACNGT 1 cut(s) 533
HpyCH4V TGCA 2 cut(s) 383, 850
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 310, 389, 411, 464, 856
HpyF3I CTNAG 2 cut(s) 85, 632
Hsp92II CATG 4 cut(s) 23, 102, 439, 643
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
LmnI GCTCC 4 cut(s) 160, 203, 706, 786
Lsp1109I GCAGC 7 cut(s) 2, 196, 389, 392, 395, 401, 467
LweI GCATC 4 cut(s) 93, 205, 676, 746
MaeI CTAG 4 cut(s) 29, 449, 690, 696
MaeIII GTNAC 3 cut(s) 361, 430, 837
MalI GATC 7 cut(s) 83, 222, 227, 483, 501, 541, 745
MboI GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
MboII GAAGA 4 cut(s) 50, 494, 563, 686
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 5 cut(s) 200, 585, 711, 791, 821
MluCI AATT 4 cut(s) 10, 393, 477, 725
MlyI GAGTC 2 cut(s) 212, 763
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 7 cut(s) 50, 59, 181, 193, 197, 265, 629
MseI TTAA 4 cut(s) 396, 476, 716, 728
MspR9I CCNGG 1 cut(s) 316
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 2 cut(s) 467, 838
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 6 cut(s) 215, 310, 389, 411, 464, 856
NdeII GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
NlaIII CATG 4 cut(s) 23, 102, 439, 643
NlaIV GGNNCC 2 cut(s) 119, 708
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 1 cut(s) 837
NspI RCATGY 1 cut(s) 643
PaeI GCATGC 1 cut(s) 643
PctI GAATGC 2 cut(s) 467, 838
PfeI GAWTC 2 cut(s) 167, 826
PkrI GCNGC 9 cut(s) 6, 17, 211, 385, 388, 391, 404, 407, 457
PleI GAGTC 2 cut(s) 211, 763
PpsI GAGTC 2 cut(s) 211, 763
PshBI ATTAAT 1 cut(s) 396
Psp124BI GAGCTC 3 cut(s) 200, 791, 821
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 119, 708
PspPI GGNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 499
SacI GAGCTC 3 cut(s) 200, 791, 821
SaqAI TTAA 4 cut(s) 396, 476, 716, 728
SatI GCNGC 9 cut(s) 5, 16, 210, 384, 387, 390, 403, 406, 456
Sau3AI GATC 7 cut(s) 81, 220, 225, 481, 499, 539, 743
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 2 cut(s) 212, 763
ScrFI CCNGG 1 cut(s) 316
SduI GDGCHC 5 cut(s) 200, 585, 711, 791, 821
SfaNI GCATC 4 cut(s) 93, 205, 676, 746
SfcI CTRYAG 1 cut(s) 568
SphI GCATGC 1 cut(s) 643
Sse9I AATT 4 cut(s) 10, 393, 477, 725
SsiI CCGC 6 cut(s) 4, 140, 146, 212, 309, 387
SspI AATATT 1 cut(s) 559
SspMI CTAG 4 cut(s) 29, 449, 690, 696
SstI GAGCTC 3 cut(s) 200, 791, 821
StyD4I CCNGG 1 cut(s) 314
StyI CCWWGG 1 cut(s) 750
TaaI ACNGT 1 cut(s) 533
TaqI TCGA 2 cut(s) 80, 267
TasI AATT 4 cut(s) 10, 393, 477, 725
TauI GCSGC 2 cut(s) 7, 389
TfiI GAWTC 2 cut(s) 167, 826
Tru1I TTAA 4 cut(s) 396, 476, 716, 728
Tru9I TTAA 4 cut(s) 396, 476, 716, 728
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 1 cut(s) 837
TseI GCWGC 7 cut(s) 15, 209, 383, 389, 402, 405, 455
Tsp45I GTSAC 1 cut(s) 837
TspDTI ATGAA 4 cut(s) 58, 310, 358, 460
TspGWI ACGGA 1 cut(s) 808
TspRI CASTG 1 cut(s) 119
VspI ATTAAT 1 cut(s) 396
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 4 cut(s) 29, 449, 690, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.