MD10G1171300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011210
Physical Location & Seq
Forward (+)
26414606 .. 26417362
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1171300.v1.1.491

Sequence Viewer

Length: 864 bp
ATGCGGCCCAATTGCTGCCACATATCCCTAGCTTTTGTGCTCAAATTACTCAACTTTCTTCAAGCCTTCATCGGCGTCTCAATCCTAGTATACTCGGCATGGATGCTCAACCAGTGGAACCACCATATTCCGATAGTCCCGCCACCGCCTTCAGCTCCTTCCCCTGACTCTTCGTCCTTCTCCTCCCTCCGATTGAGCTCCGACTCGGCGGCGGTTGCCGATCAGATCACGCCGTTGGATTTTGCAGTCCATTTGGTGTCTGGGTTCGATGAGGGATCGGGGTTCGGATTGAACTCGTTCAAGCTTCCAGCGCCATGGTTCATCTACTCTTTTATGGGACTGGGGATTTTGCTCTGTTGCGTTACATTCATAGGTTGTATTGCAGCTACAGCAATTAATGGCTGCTGCCTTTGCTTTTACACAATACTTATAACCATGCTCACTCTACTAGAAGCAGCTTTGGTGGCATTCATTTTAATTGATCGCCGTTGGGAAAAGGATCTTCCATTTGACCCAACTGGTCAACTTGACAGTCTTCGATCTTTTGTTAAAGAGAATATTGACATCTGTAGGTGGGTCGGGCTCACTGTCATTGTAATACAGGCTTTATCTCTGCTACTCTCAATAACTCTGCGAGGCATGCTTTCTATTTGCAAACCTGACTATGATGTTGAAGATGATTCTGATGCTAGGGCTAGAACTCGGGAGCCCCTGTTGAACCAAAAATTAAACCAAGCATCTGGATCAACCAAGGGTGATGGGAGAGGGACTCACTCTGATATTTGGAGCTCACGGATAAGAGAGAAGTATGGATTGAGCTCTCTGAATGAGAATGCGTCAGCGAGTATGAAATCCAAGGAGTGA

Protein Analysis

288

Amino Acids

31.54

Weight (kDa)

5.96

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 164 3e-06 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20230 AT4G28770 AT4G28770
fragaria_vesca FvH4_2g19890 FvH4_2g19890
malus_domestica MD05G1183500.v1.1 MD10G1171300.v1.1
prunus_persica Prupe.8G209700_v2.0.a1
pyrus_communis pycom05g16850 pycom10g14770
rosa_chinensis RchiOBHm_Chr6g0285581
rosa_laevigata RLG00000012625
rosa_multiflora Rmu_sc0012495.1_g000001
rosa_roxburghii Rroxscaffold_7G00182930
rosa_rugosa Rorug06G0175800
rosa_samantha Rh6AG287300 Rh6BG288500 Rh6CG289700 Rh6DG282500
rosa_wichuraiana Rw6G024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccI GTMKAC 1 cut(s) 90
AciI CCGC 5 cut(s) 4, 140, 146, 209, 212
AclWI GGATC 3 cut(s) 283, 507, 751
AcuI CTGAAG 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 75
AgsI TTSAA 5 cut(s) 62, 292, 301, 674, 718
AhdI GACNNNNNGTC 1 cut(s) 172
AluBI AGCT 8 cut(s) 32, 155, 198, 304, 386, 458, 789, 819
AluI AGCT 8 cut(s) 32, 155, 198, 304, 386, 458, 789, 819
Alw21I GWGCWC 4 cut(s) 42, 200, 791, 821
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 3 cut(s) 283, 507, 751
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 5 cut(s) 15, 383, 402, 405, 455
AseI ATTAAT 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 767
AvaI CYCGRG 1 cut(s) 702
BanII GRGCYC 5 cut(s) 200, 585, 711, 791, 821
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 4 cut(s) 42, 200, 791, 821
BbvI GCAGC 5 cut(s) 2, 389, 392, 395, 467
BccI CCATC 1 cut(s) 752
BceAI ACGGC 2 cut(s) 217, 471
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 5 cut(s) 29, 86, 449, 690, 696
BfmI CTRYAG 2 cut(s) 387, 568
BfoI RGCGCY 1 cut(s) 314
BisI GCNGC 7 cut(s) 5, 16, 210, 384, 403, 406, 456
BlsI GCNGC 7 cut(s) 6, 17, 211, 385, 404, 407, 457
BmeRI GACNNNNNGTC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 119, 708
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 3 cut(s) 93, 676, 746
BmuI ACTGGG 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 527
BplI GAGNNNNNCTC 2 cut(s) 754, 786
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 314, 750, 855
BsaXI ACNNNNNCTCC 2 cut(s) 698, 728
Bse1I ACTGG 3 cut(s) 112, 345, 523
BseDI CCNNGG 3 cut(s) 314, 750, 855
BseGI GGATG 1 cut(s) 108
BseNI ACTGG 3 cut(s) 112, 345, 523
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 5 cut(s) 2, 389, 392, 395, 467
BshFI GGCC 1 cut(s) 7
BsiHKAI GWGCWC 4 cut(s) 42, 200, 791, 821
BsiHKCI CYCGRG 1 cut(s) 702
BslFI GGGAC 3 cut(s) 122, 351, 781
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 3 cut(s) 122, 351, 781
BsmI GAATGC 2 cut(s) 467, 838
BsnI GGCC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 702
Bsp1286I GDGCHC 6 cut(s) 42, 200, 585, 711, 791, 821
Bsp143I GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 5 cut(s) 4, 140, 146, 209, 212
BspANI GGCC 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 119, 708
BspPI GGATC 3 cut(s) 283, 507, 751
BsrI ACTGG 3 cut(s) 112, 345, 523
BssECI CCNNGG 3 cut(s) 314, 750, 855
BssMI GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
BssNAI GTATAC 1 cut(s) 91
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 3 cut(s) 314, 750, 855
Bst1107I GTATAC 1 cut(s) 91
Bst4CI ACNGT 2 cut(s) 533, 589
Bst6I CTCTTC 1 cut(s) 175
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 641
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 7 cut(s) 223, 228, 278, 484, 502, 542, 746
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
BstMWI GCNNNNNNNGC 6 cut(s) 215, 310, 389, 411, 464, 640
BstNSI RCATGY 1 cut(s) 643
BstSFI CTRYAG 2 cut(s) 387, 568
BstV1I GCAGC 5 cut(s) 2, 389, 392, 395, 467
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 499
BstXI CCANNNNNNTGG 2 cut(s) 315, 740
BstYI RGATCY 1 cut(s) 499
BstZ17I GTATAC 1 cut(s) 91
BsuRI GGCC 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 119, 585
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 6
CseI GACGC 2 cut(s) 64, 825
CviAII CATG 4 cut(s) 99, 315, 436, 640
DpnI GATC 7 cut(s) 222, 227, 277, 483, 501, 541, 745
DpnII GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
DriI GACNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 175
Eam1105I GACNNNNNGTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 175
Ecl136II GAGCTC 3 cut(s) 198, 789, 819
Eco130I CCWWGG 3 cut(s) 314, 750, 855
Eco24I GRGCYC 5 cut(s) 200, 585, 711, 791, 821
Eco53kI GAGCTC 3 cut(s) 198, 789, 819
Eco57I CTGAAG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 702
EcoICRI GAGCTC 3 cut(s) 198, 789, 819
EcoT14I CCWWGG 3 cut(s) 314, 750, 855
EcoT38I GRGCYC 5 cut(s) 200, 585, 711, 791, 821
ErhI CCWWGG 3 cut(s) 314, 750, 855
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 4 cut(s) 102, 318, 439, 643
FaqI GGGAC 3 cut(s) 122, 351, 781
FatI CATG 4 cut(s) 98, 314, 435, 639
FauI CCCGC 1 cut(s) 147
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 7 cut(s) 5, 16, 210, 384, 403, 406, 456
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 5 cut(s) 200, 585, 711, 791, 821
Fsp4HI GCNGC 7 cut(s) 5, 16, 210, 384, 403, 406, 456
FspBI CTAG 5 cut(s) 29, 86, 449, 690, 696
GlaI GCGC 1 cut(s) 312
GluI GCNGC 7 cut(s) 5, 16, 210, 384, 403, 406, 456
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 1 cut(s) 7
HgaI GACGC 2 cut(s) 64, 825
HhaI GCGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 4 cut(s) 102, 318, 439, 643
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 4 cut(s) 167, 203, 680, 769
HphI GGTGA 1 cut(s) 767
Hpy166II GTNNAC 2 cut(s) 91, 524
Hpy188I TCNGA 8 cut(s) 132, 191, 202, 225, 287, 685, 778, 825
Hpy188III TCNNGA 2 cut(s) 704, 741
Hpy8I GTNNAC 2 cut(s) 91, 524
HpyAV CCTTC 4 cut(s) 76, 159, 168, 187
HpyCH4III ACNGT 2 cut(s) 533, 589
HpyCH4V TGCA 3 cut(s) 245, 383, 654
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 310, 389, 411, 464, 640
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 4 cut(s) 102, 318, 439, 643
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
LmnI GCTCC 4 cut(s) 160, 203, 706, 786
Lsp1109I GCAGC 5 cut(s) 2, 389, 392, 395, 467
LweI GCATC 3 cut(s) 93, 676, 746
MaeI CTAG 5 cut(s) 29, 86, 449, 690, 696
MaeIII GTNAC 1 cut(s) 361
MalI GATC 7 cut(s) 222, 227, 277, 483, 501, 541, 745
MboI GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
MboII GAAGA 5 cut(s) 50, 162, 494, 527, 686
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 6 cut(s) 42, 200, 585, 711, 791, 821
MluCI AATT 5 cut(s) 10, 44, 393, 477, 725
MlyI GAGTC 3 cut(s) 161, 197, 763
MmeI TCCRAC 2 cut(s) 216, 225
MnlI CCTC 5 cut(s) 193, 197, 265, 629, 758
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 4 cut(s) 396, 476, 549, 728
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 2 cut(s) 467, 838
MwoI GCNNNNNNNGC 6 cut(s) 215, 310, 389, 411, 464, 640
NcoI CCATGG 1 cut(s) 314
NdeII GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
NlaIII CATG 4 cut(s) 102, 318, 439, 643
NlaIV GGNNCC 2 cut(s) 119, 708
NmeAIII GCCGAG 2 cut(s) 74, 185
NspI RCATGY 1 cut(s) 643
PaeI GCATGC 1 cut(s) 643
PctI GAATGC 2 cut(s) 467, 838
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 680
PkrI GCNGC 7 cut(s) 6, 17, 211, 385, 404, 407, 457
PleI GAGTC 3 cut(s) 161, 197, 763
PpsI GAGTC 3 cut(s) 161, 197, 763
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 1 cut(s) 431
Psp124BI GAGCTC 3 cut(s) 200, 791, 821
PspN4I GGNNCC 2 cut(s) 119, 708
PspPI GGNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 499
SacI GAGCTC 3 cut(s) 200, 791, 821
SaqAI TTAA 4 cut(s) 396, 476, 549, 728
SatI GCNGC 7 cut(s) 5, 16, 210, 384, 403, 406, 456
Sau3AI GATC 7 cut(s) 220, 225, 275, 481, 499, 539, 743
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 3 cut(s) 161, 197, 763
SduI GDGCHC 6 cut(s) 42, 200, 585, 711, 791, 821
SfaNI GCATC 3 cut(s) 93, 676, 746
SfcI CTRYAG 2 cut(s) 387, 568
SphI GCATGC 1 cut(s) 643
Sse9I AATT 5 cut(s) 10, 44, 393, 477, 725
SsiI CCGC 5 cut(s) 4, 140, 146, 209, 212
SspI AATATT 1 cut(s) 559
SspMI CTAG 5 cut(s) 29, 86, 449, 690, 696
SstI GAGCTC 3 cut(s) 200, 791, 821
StyI CCWWGG 3 cut(s) 314, 750, 855
TaaI ACNGT 2 cut(s) 533, 589
TaqI TCGA 2 cut(s) 267, 538
TasI AATT 5 cut(s) 10, 44, 393, 477, 725
TauI GCSGC 2 cut(s) 7, 212
TfiI GAWTC 1 cut(s) 680
Tru1I TTAA 4 cut(s) 396, 476, 549, 728
Tru9I TTAA 4 cut(s) 396, 476, 549, 728
TscAI CASTG 2 cut(s) 119, 592
TseI GCWGC 5 cut(s) 15, 383, 402, 405, 455
TspDTI ATGAA 5 cut(s) 58, 310, 358, 460, 863
TspGWI ACGGA 1 cut(s) 808
TspRI CASTG 2 cut(s) 119, 592
VspI ATTAAT 1 cut(s) 396
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 5 cut(s) 29, 86, 449, 690, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.