pycom10g14770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011210
Physical Location & Seq
Forward (+)
18185971 .. 18188226
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g14770.3

Sequence Viewer

Length: 783 bp
ATGCGGCCCAATTGCTGCCACATATCCCTAGCTTTCGTGCTCAAATTACTCAACTTTCTTCAAGCCTTCATCGGCGTCTCAATCCTTGTCTACTCGGCATGGATGCTCAACCAGTGGAACCACCATATTCCGATAGTCCCGCCACCGCCTTCAGCTCCTTCCCCTGACTCTTCGTCCTTCTCCTCCCTCCGATTGAGCTCCGACTCGGCGGCGCTTGCCGATCAGATCACGCCGTTGGATTTTGCAGTCCATTTGGTGTCTGGATCCGATGAGGGATTGGGGTTCGGATTGAACTCGTTCAAGCTTCCAGCGCCATGGTTCATCTACTCTTTTATGGGACTGGGAATTTTGCTCTGTTGCGTTAATTTCATAGGTTGTATTGCAGCTACAGCAATTAATGGCTGCTGCCTTTGCTTTGATCTTCCATTTGACCCAACTGGTCAACTTGACAGTCTTCGATCTTTTGTTAAAGAGAATATTGACATCTGTAGGTGGGTCGGGCTCACTGTCATTGTAATACAGGCTTTATCTCTGCTACTCTCAATAACTCTGCGAGGCATGCTTTCTACTCGCAAACCTGACTATGATGTTGAAGATGATTCTGATGCTAGGGCTAGAACTCGGGAGCCCCTGTTAAACCAAAAATTAAACCAAGCATCTGGATCAACCAAAGGTGATGGGAGAGGGAGTCACTCTGACATCTGGAGCTCACGGATAAGAGAGAAGTATGGATTGAGCTCTCTGAATCAGAATGCGTCAGCGAGTATGAAATCCAAGGAGTGA

Protein Analysis

261

Amino Acids

28.37

Weight (kDa)

6.5

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20230 AT4G28770 AT4G28770
fragaria_vesca FvH4_2g19890 FvH4_2g19890
malus_domestica MD05G1183500.v1.1 MD10G1171300.v1.1
prunus_persica Prupe.8G209700_v2.0.a1
pyrus_communis pycom05g16850 pycom10g14770
rosa_chinensis RchiOBHm_Chr6g0285581
rosa_laevigata RLG00000012625
rosa_multiflora Rmu_sc0012495.1_g000001
rosa_roxburghii Rroxscaffold_7G00182930
rosa_rugosa Rorug06G0175800
rosa_samantha Rh6AG287300 Rh6BG288500 Rh6CG289700 Rh6DG282500
rosa_wichuraiana Rw6G024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AciI CCGC 4 cut(s) 4, 140, 146, 209
AclWI GGATC 3 cut(s) 258, 271, 670
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 75
AgsI TTSAA 4 cut(s) 62, 292, 301, 593
AhdI GACNNNNNGTC 1 cut(s) 172
AluBI AGCT 7 cut(s) 32, 155, 198, 304, 386, 708, 738
AluI AGCT 7 cut(s) 32, 155, 198, 304, 386, 708, 738
Alw21I GWGCWC 4 cut(s) 42, 200, 710, 740
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 3 cut(s) 258, 271, 670
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 4 cut(s) 15, 383, 402, 405
ApoI RAATTY 1 cut(s) 345
AseI ATTAAT 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 2 cut(s) 214, 313
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 621
BamHI GGATCC 1 cut(s) 263
BanII GRGCYC 5 cut(s) 200, 504, 630, 710, 740
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 4 cut(s) 42, 200, 710, 740
BbvI GCAGC 4 cut(s) 2, 389, 392, 395
BccI CCATC 1 cut(s) 671
BceAI ACGGC 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 3 cut(s) 29, 609, 615
BfmI CTRYAG 2 cut(s) 387, 487
BfoI RGCGCY 2 cut(s) 215, 314
BisI GCNGC 6 cut(s) 5, 16, 210, 384, 403, 406
BlsI GCNGC 6 cut(s) 6, 17, 211, 385, 404, 407
BmeRI GACNNNNNGTC 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 3 cut(s) 119, 265, 627
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 3 cut(s) 93, 595, 665
BmuI ACTGGG 1 cut(s) 350
BpiI GAAGAC 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 724
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 314, 774
BsaXI ACNNNNNCTCC 4 cut(s) 617, 647, 673, 703
Bse1I ACTGG 3 cut(s) 112, 345, 442
BseDI CCNNGG 2 cut(s) 314, 774
BseGI GGATG 1 cut(s) 108
BseNI ACTGG 3 cut(s) 112, 345, 442
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 4 cut(s) 2, 389, 392, 395
BshFI GGCC 1 cut(s) 7
BsiHKAI GWGCWC 4 cut(s) 42, 200, 710, 740
BsiHKCI CYCGRG 1 cut(s) 621
BslFI GGGAC 2 cut(s) 122, 351
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 2 cut(s) 122, 351
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 1 cut(s) 7
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 6 cut(s) 42, 200, 504, 630, 710, 740
Bsp143I GATC 6 cut(s) 220, 225, 263, 418, 458, 662
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 4 cut(s) 4, 140, 146, 209
BspANI GGCC 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 119, 265, 627
BspPI GGATC 3 cut(s) 258, 271, 670
BsrI ACTGG 3 cut(s) 112, 345, 442
BssECI CCNNGG 2 cut(s) 314, 774
BssMI GATC 6 cut(s) 220, 225, 263, 418, 458, 662
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 2 cut(s) 314, 774
Bst4CI ACNGT 2 cut(s) 452, 508
Bst6I CTCTTC 1 cut(s) 175
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 2 cut(s) 216, 560
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 108
BstH2I RGCGCY 2 cut(s) 215, 314
BstHHI GCGC 2 cut(s) 214, 313
BstKTI GATC 6 cut(s) 223, 228, 266, 421, 461, 665
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 6 cut(s) 220, 225, 263, 418, 458, 662
BstMWI GCNNNNNNNGC 5 cut(s) 215, 310, 389, 411, 559
BstNSI RCATGY 1 cut(s) 562
BstSFI CTRYAG 2 cut(s) 387, 487
BstV1I GCAGC 4 cut(s) 2, 389, 392, 395
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 263
BstXI CCANNNNNNTGG 2 cut(s) 315, 659
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 119, 504
Cac8I GCNNGC 2 cut(s) 216, 560
CfoI GCGC 2 cut(s) 214, 313
Cfr13I GGNCC 1 cut(s) 6
CseI GACGC 2 cut(s) 64, 744
CviAII CATG 3 cut(s) 99, 315, 559
DpnI GATC 6 cut(s) 222, 227, 265, 420, 460, 664
DpnII GATC 6 cut(s) 220, 225, 263, 418, 458, 662
DriI GACNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 175
Eam1105I GACNNNNNGTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 175
Ecl136II GAGCTC 3 cut(s) 198, 708, 738
Eco130I CCWWGG 2 cut(s) 314, 774
Eco24I GRGCYC 5 cut(s) 200, 504, 630, 710, 740
Eco53kI GAGCTC 3 cut(s) 198, 708, 738
Eco57I CTGAAG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 621
EcoICRI GAGCTC 3 cut(s) 198, 708, 738
EcoT14I CCWWGG 2 cut(s) 314, 774
EcoT38I GRGCYC 5 cut(s) 200, 504, 630, 710, 740
ErhI CCWWGG 2 cut(s) 314, 774
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 3 cut(s) 102, 318, 562
FaqI GGGAC 2 cut(s) 122, 351
FatI CATG 3 cut(s) 98, 314, 558
FauI CCCGC 1 cut(s) 147
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 6 cut(s) 5, 16, 210, 384, 403, 406
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 5 cut(s) 200, 504, 630, 710, 740
Fsp4HI GCNGC 6 cut(s) 5, 16, 210, 384, 403, 406
FspBI CTAG 3 cut(s) 29, 609, 615
GlaI GCGC 2 cut(s) 213, 312
GluI GCNGC 6 cut(s) 5, 16, 210, 384, 403, 406
GsuI CTGGAG 1 cut(s) 724
HaeII RGCGCY 2 cut(s) 215, 314
HaeIII GGCC 1 cut(s) 7
HgaI GACGC 2 cut(s) 64, 744
HhaI GCGC 2 cut(s) 214, 313
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 102, 318, 562
Hin6I GCGC 2 cut(s) 212, 311
HinP1I GCGC 2 cut(s) 212, 311
HincII GTYRAC 1 cut(s) 443
HindII GTYRAC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 5 cut(s) 167, 203, 599, 688, 745
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 2 cut(s) 91, 443
Hpy188III TCNNGA 4 cut(s) 261, 623, 660, 703
Hpy8I GTNNAC 2 cut(s) 91, 443
HpyAV CCTTC 4 cut(s) 76, 159, 168, 187
HpyCH4III ACNGT 2 cut(s) 452, 508
HpyCH4V TGCA 2 cut(s) 245, 383
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 310, 389, 411, 559
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 102, 318, 562
HspAI GCGC 2 cut(s) 212, 311
Kzo9I GATC 6 cut(s) 220, 225, 263, 418, 458, 662
LmnI GCTCC 4 cut(s) 160, 203, 625, 705
Lsp1109I GCAGC 4 cut(s) 2, 389, 392, 395
LweI GCATC 3 cut(s) 93, 595, 665
MaeI CTAG 3 cut(s) 29, 609, 615
MaeIII GTNAC 1 cut(s) 689
MalI GATC 6 cut(s) 222, 227, 265, 420, 460, 664
MboI GATC 6 cut(s) 220, 225, 263, 418, 458, 662
MboII GAAGA 5 cut(s) 50, 162, 413, 446, 605
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 6 cut(s) 42, 200, 504, 630, 710, 740
MluCI AATT 6 cut(s) 10, 44, 345, 364, 393, 644
MlyI GAGTC 3 cut(s) 161, 197, 697
MmeI TCCRAC 2 cut(s) 216, 225
MnlI CCTC 5 cut(s) 193, 197, 265, 548, 677
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 5 cut(s) 363, 396, 468, 635, 647
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 757
MwoI GCNNNNNNNGC 5 cut(s) 215, 310, 389, 411, 559
NcoI CCATGG 1 cut(s) 314
NdeII GATC 6 cut(s) 220, 225, 263, 418, 458, 662
NlaIII CATG 3 cut(s) 102, 318, 562
NlaIV GGNNCC 3 cut(s) 119, 265, 627
NmeAIII GCCGAG 2 cut(s) 74, 185
NmuCI GTSAC 1 cut(s) 689
NspI RCATGY 1 cut(s) 562
PaeI GCATGC 1 cut(s) 562
PctI GAATGC 1 cut(s) 757
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 599, 745
PkrI GCNGC 6 cut(s) 6, 17, 211, 385, 404, 407
PleI GAGTC 3 cut(s) 161, 197, 696
PpsI GAGTC 3 cut(s) 161, 197, 696
PshBI ATTAAT 1 cut(s) 396
Psp124BI GAGCTC 3 cut(s) 200, 710, 740
PspN4I GGNNCC 3 cut(s) 119, 265, 627
PspPI GGNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 263
SacI GAGCTC 3 cut(s) 200, 710, 740
SaqAI TTAA 5 cut(s) 363, 396, 468, 635, 647
SatI GCNGC 6 cut(s) 5, 16, 210, 384, 403, 406
Sau3AI GATC 6 cut(s) 220, 225, 263, 418, 458, 662
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 3 cut(s) 161, 197, 697
SduI GDGCHC 6 cut(s) 42, 200, 504, 630, 710, 740
SfaNI GCATC 3 cut(s) 93, 595, 665
SfcI CTRYAG 2 cut(s) 387, 487
SphI GCATGC 1 cut(s) 562
Sse9I AATT 6 cut(s) 10, 44, 345, 364, 393, 644
SsiI CCGC 4 cut(s) 4, 140, 146, 209
SspI AATATT 1 cut(s) 478
SspMI CTAG 3 cut(s) 29, 609, 615
SstI GAGCTC 3 cut(s) 200, 710, 740
StyI CCWWGG 2 cut(s) 314, 774
TaaI ACNGT 2 cut(s) 452, 508
TaqI TCGA 1 cut(s) 457
TasI AATT 6 cut(s) 10, 44, 345, 364, 393, 644
TauI GCSGC 2 cut(s) 7, 212
TfiI GAWTC 2 cut(s) 599, 745
Tru1I TTAA 5 cut(s) 363, 396, 468, 635, 647
Tru9I TTAA 5 cut(s) 363, 396, 468, 635, 647
TscAI CASTG 2 cut(s) 119, 511
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 4 cut(s) 15, 383, 402, 405
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 4 cut(s) 58, 310, 358, 782
TspGWI ACGGA 1 cut(s) 727
TspRI CASTG 2 cut(s) 119, 511
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 1 cut(s) 345
XceI RCATGY 1 cut(s) 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 3 cut(s) 29, 609, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.