Rh6AG287300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011210
Physical Location & Seq
Forward (+)
48367238 .. 48369991
2754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG287300.1

Sequence Viewer

Length: 642 bp
ATGCCGAAGAATTGCTGCCACGTATGCTTATCTTTCGTGCTCAAATTCCTCAACTTTCTTCAAGCTTTCGTCGGCGTCGCGATCCTTGTCTACTCGGCGTGGATGCTTGATCAGTGGAACCATCACATTCCGGTGTTCCCACCGCCCTCCGCGCCTTCCCCGGACTCTCCTTTCTCACTTTCGTTGGGCTCCGACGCCGGCGCGGCTTCCGATCAGATCACGCCGTTGGATTTTGCAGTCCGGTTGGTGTCTGGGGTCGATGACGGCATGGCGTTTCAATTGAATGCAGTGAAGCTCCCTGCTCCTTGGTTCATCTACTCTTTTATGGGAGTGGGGGCTTTGCTCTGTTGCATTACTTTCATAGGTTGTATCGCAGCTGAATCAATCAACGGCTGCTGCCTGTGTTTCTACAGTCTGCTCATAACGGTACTAACTCTAGTGGAAGCAGCTCTGGTAGCGTTCATAGCTATTGATCACCATTGGGACAAGGATCTTCCACTTGACCCAACTGGTGAACTTGACAGTCTTCGATCTTTTATTGAAGAGAACATTGACATCTGTAAGTGCGTTGGGATAGCTGTTATTGGAATACAGGTATGCTGCAGTTATAATGATGACTTTGTTCCTCTCTTGATTGCATAG

Protein Analysis

213

Amino Acids

23.07

Weight (kDa)

4.33

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 161 8.8e-09 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011210)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20230 AT4G28770 AT4G28770
fragaria_vesca FvH4_2g19890 FvH4_2g19890
malus_domestica MD05G1183500.v1.1 MD10G1171300.v1.1
prunus_persica Prupe.8G209700_v2.0.a1
pyrus_communis pycom05g16850 pycom10g14770
rosa_chinensis RchiOBHm_Chr6g0285581
rosa_laevigata RLG00000012625
rosa_multiflora Rmu_sc0012495.1_g000001
rosa_roxburghii Rroxscaffold_7G00182930
rosa_rugosa Rorug06G0175800
rosa_samantha Rh6AG287300 Rh6BG288500 Rh6CG289700 Rh6DG282500
rosa_wichuraiana Rw6G024700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AccI GTMKAC 1 cut(s) 90
AccII CGCG 3 cut(s) 80, 152, 203
AciI CCGC 3 cut(s) 143, 150, 203
AclWI GGATC 2 cut(s) 76, 498
AcsI RAATTY 1 cut(s) 44
AcyI GRCGYC 2 cut(s) 75, 195
AfaI GTAC 1 cut(s) 429
AgsI TTSAA 4 cut(s) 62, 278, 283, 542
AluBI AGCT 6 cut(s) 65, 295, 377, 449, 467, 578
AluI AGCT 6 cut(s) 65, 295, 377, 449, 467, 578
Alw21I GWGCWC 1 cut(s) 42
AlwI GGATC 2 cut(s) 76, 498
ApeKI GCWGC 6 cut(s) 15, 374, 393, 396, 446, 600
ApoI RAATTY 1 cut(s) 44
AspLEI GCGC 2 cut(s) 154, 203
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 467, 524
BanII GRGCYC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 6 cut(s) 2, 380, 383, 386, 458, 587
BccI CCATC 1 cut(s) 129
BceAI ACGGC 3 cut(s) 208, 280, 406
BclI TGATCA 2 cut(s) 109, 472
BcnI CCSGG 1 cut(s) 161
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 2 cut(s) 409, 601
BglI GCCNNNNNGGC 1 cut(s) 203
BisI GCNGC 7 cut(s) 16, 204, 375, 394, 397, 447, 601
BlsI GCNGC 7 cut(s) 17, 205, 376, 395, 398, 448, 602
Bme1390I CCNGG 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 119, 190
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 93
BpiI GAAGAC 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 22
BsaHI GRCGYC 2 cut(s) 75, 195
BsaJI CCNNGG 2 cut(s) 159, 305
BsaWI WCCGGW 2 cut(s) 130, 240
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 514
BseDI CCNNGG 2 cut(s) 159, 305
BseGI GGATG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 514
BseXI GCAGC 6 cut(s) 2, 380, 383, 386, 458, 587
Bsh1236I CGCG 3 cut(s) 80, 152, 203
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 4 cut(s) 131, 161, 198, 241
BslFI GGGAC 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 42, 191
Bsp143I GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
Bsp68I TCGCGA 1 cut(s) 80
BspACI CCGC 3 cut(s) 143, 150, 203
BspFNI CGCG 3 cut(s) 80, 152, 203
BspLI GGNNCC 2 cut(s) 119, 190
BspMAI CTGCAG 1 cut(s) 605
BspPI GGATC 2 cut(s) 76, 498
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 159, 305
BssMI GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
BssNI GRCGYC 2 cut(s) 75, 195
BssT1I CCWWGG 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 413, 427, 524
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 2 cut(s) 75, 195
BstBAI YACGTR 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 199
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 3 cut(s) 80, 152, 203
BstHHI GCGC 2 cut(s) 154, 203
BstKTI GATC 7 cut(s) 84, 112, 214, 219, 475, 493, 533
BstMBI GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
BstMWI GCNNNNNNNGC 5 cut(s) 24, 151, 203, 455, 464
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 2 cut(s) 409, 601
BstUI CGCG 3 cut(s) 80, 152, 203
BstV1I GCAGC 6 cut(s) 2, 380, 383, 386, 458, 587
BstV2I GAAGAC 1 cut(s) 518
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 119, 294
BtuMI TCGCGA 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 2 cut(s) 154, 203
Cfr10I RCCGGY 1 cut(s) 197
CseI GACGC 2 cut(s) 64, 203
Csp6I GTAC 1 cut(s) 428
CviAII CATG 1 cut(s) 268
CviQI GTAC 1 cut(s) 428
DpnI GATC 7 cut(s) 83, 111, 213, 218, 474, 492, 532
DpnII GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 305
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 1 cut(s) 271
FaiI YATR 9 cut(s) 25, 269, 326, 362, 422, 464, 598, 609, 640
FaqI GGGAC 1 cut(s) 497
FatI CATG 1 cut(s) 267
FbaI TGATCA 2 cut(s) 109, 472
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 7 cut(s) 16, 204, 375, 394, 397, 447, 601
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 7 cut(s) 16, 204, 375, 394, 397, 447, 601
FspBI CTAG 1 cut(s) 437
GlaI GCGC 2 cut(s) 153, 202
GluI GCNGC 7 cut(s) 16, 204, 375, 394, 397, 447, 601
HapII CCGG 4 cut(s) 131, 161, 198, 241
HgaI GACGC 2 cut(s) 64, 203
HhaI GCGC 2 cut(s) 154, 203
Hin1I GRCGYC 2 cut(s) 75, 195
Hin1II CATG 1 cut(s) 271
Hin6I GCGC 2 cut(s) 152, 201
HinP1I GCGC 2 cut(s) 152, 201
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 2 cut(s) 164, 380
HpaII CCGG 4 cut(s) 131, 161, 198, 241
HphI GGTGA 2 cut(s) 467, 524
Hpy166II GTNNAC 2 cut(s) 91, 515
Hpy188I TCNGA 3 cut(s) 193, 211, 216
Hpy188III TCNNGA 2 cut(s) 79, 631
Hpy8I GTNNAC 2 cut(s) 91, 515
Hpy99I CGWCG 3 cut(s) 74, 80, 197
HpyAV CCTTC 1 cut(s) 165
HpyCH4III ACNGT 3 cut(s) 413, 427, 524
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 5 cut(s) 236, 287, 351, 603, 638
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 151, 203, 455, 464
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 2 cut(s) 75, 195
Hsp92II CATG 1 cut(s) 271
HspAI GCGC 2 cut(s) 152, 201
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 109, 472
Kzo9I GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
LmnI GCTCC 3 cut(s) 194, 300, 307
Lsp1109I GCAGC 6 cut(s) 2, 380, 383, 386, 458, 587
LweI GCATC 1 cut(s) 93
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 21
MalI GATC 7 cut(s) 83, 111, 213, 218, 474, 492, 532
MboI GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
MboII GAAGA 5 cut(s) 19, 50, 485, 518, 554
MfeI CAATTG 1 cut(s) 278
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 2 cut(s) 42, 191
MluCI AATT 3 cut(s) 10, 44, 278
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 2 cut(s) 207, 216
MnlI CCTC 3 cut(s) 59, 157, 636
MreI CGCCGGCG 1 cut(s) 197
MroNI GCCGGC 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 4 cut(s) 131, 161, 198, 241
MspR9I CCNGG 1 cut(s) 161
MunI CAATTG 1 cut(s) 278
Mva1269I GAATGC 1 cut(s) 289
MvnI CGCG 3 cut(s) 80, 152, 203
MwoI GCNNNNNNNGC 5 cut(s) 24, 151, 203, 455, 464
NaeI GCCGGC 1 cut(s) 199
NciI CCSGG 1 cut(s) 161
NdeII GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
NgoMIV GCCGGC 1 cut(s) 197
NlaIII CATG 1 cut(s) 271
NlaIV GGNNCC 2 cut(s) 119, 190
NmeAIII GCCGAG 1 cut(s) 74
NruI TCGCGA 1 cut(s) 80
PctI GAATGC 1 cut(s) 289
PdiI GCCGGC 1 cut(s) 199
PfeI GAWTC 1 cut(s) 380
PkrI GCNGC 7 cut(s) 17, 205, 376, 395, 398, 448, 602
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Ppu21I YACGTR 1 cut(s) 22
PsiI TTATAA 1 cut(s) 609
PspN4I GGNNCC 2 cut(s) 119, 190
PstI CTGCAG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 377
RruI TCGCGA 1 cut(s) 80
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 131
SatI GCNGC 7 cut(s) 16, 204, 375, 394, 397, 447, 601
Sau3AI GATC 7 cut(s) 81, 109, 211, 216, 472, 490, 530
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 2 cut(s) 42, 191
SetI ASST 9 cut(s) 24, 67, 297, 367, 379, 451, 469, 580, 597
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 2 cut(s) 409, 601
SgrAI CRCCGGYG 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 131
Sse9I AATT 3 cut(s) 10, 44, 278
SsiI CCGC 3 cut(s) 143, 150, 203
SspMI CTAG 1 cut(s) 437
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 3 cut(s) 413, 427, 524
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 258, 529
TasI AATT 3 cut(s) 10, 44, 278
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 1 cut(s) 380
TscAI CASTG 2 cut(s) 119, 294
TseI GCWGC 6 cut(s) 15, 374, 393, 396, 446, 600
TspDTI ATGAA 3 cut(s) 301, 349, 451
TspRI CASTG 2 cut(s) 119, 294
XapI RAATTY 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.