AT4G30900

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010359
Physical Location & Seq
Forward (+)
15039336 .. 15042401
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30900.2

Sequence Viewer

Length: 951 bp
ATGAGTGTGTCTTTGAGTGTGATGTCATTCAATCTTCACGATGATCTACCTGAAGAAAGCCCTAATTCATGGCTCAAAAGGAAAGATTTGTGTCTCACTGTCATCACTAGTTACTCCCCCATTGTTCTCTGTACCCAACAAGGTGTGAAGTCGCAGTTGGATTATCTTCAGCAGGGGTTGCCAGCCTATGATCAATTTGGCATATCAAGAACAGGACCTGGGGATGCTAATGATGAACACTGCACCATCTTCTTCAACAAGGAAAAAGTAGAGCTGCTAGAGGGTGGAACTTTTTGGTTGTCAGAGTCTCCCTCGGTCCCTGGAAGCACGGCATGGGGTTCCGCAGTTCCTTGTATAGCGACATGGGCTACATTCCAACTTAAAGGAGCGGAGCCGCCTGGTTTTTCGTTTCAGATAGTCAACACAAACTTGGATGAAATCAGCCCTCGTGCCCGTAGGCGAAGTGCTTTACTCACATGGCAACACATAGCATCATTACCTCCCACTTTGCCGGTTGTGTACTGTGGAGGATTCAACACACAGAAGGAATCAACAACAGGGCGGTTTCTGTTGGGCAGATCTAGAGAGCATGGTGTGGTAGGAGATATGAGAGACGCATGGCCAAGTGCCCGAGTAAGAAAAAACGTCGCTCTCATAAGAACATACCATGATTTCAAAGGTGACAAGCAGGGAACGGTCGAGTTCCTGAAGCTGATATTCAGAGCGTTATGCCTCTGCTGGGACCGACAGACGCAAGATCTACACACGGATTGGATACTTTACAGAGGTAGATCTATAGTTCCTGTAATGTGCGAGATCGTAAATGATAAGATTGACGACTTGTATCCTTCATCTCACTACCCTGTGTTTGCTGAGTTTATGCTTCCTCGTTCTGTAAGAATGCTTGAACCTACTCCTCCTGTTTCTGCTCCTGCTCAAGAGGAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

316

Amino Acids

35.5

Weight (kDa)

6.0

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 274 3.4e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 389
AciI CCGC 4 cut(s) 342, 389, 395, 562
AcoI YGGCCR 1 cut(s) 620
AcuI CTGAAG 3 cut(s) 72, 152, 728
AfaI GTAC 2 cut(s) 133, 521
AfiI CCNNNNNNNGG 1 cut(s) 739
AgsI TTSAA 5 cut(s) 31, 256, 535, 676, 908
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 3 cut(s) 217, 319, 397
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 3 cut(s) 274, 712, 948
AluI AGCT 3 cut(s) 274, 712, 948
Alw26I GTCTC 3 cut(s) 98, 312, 606
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 1 cut(s) 274
AspS9I GGNCC 3 cut(s) 215, 316, 742
AsuHPI GGTGA 1 cut(s) 692
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 3 cut(s) 215, 316, 742
BaeGI GKGCMC 2 cut(s) 454, 631
BalI TGGCCA 1 cut(s) 622
BauI CACGAG 1 cut(s) 447
BbvI GCAGC 1 cut(s) 261
BccI CCATC 1 cut(s) 254
BceAI ACGGC 1 cut(s) 345
BciT130I CCWGG 3 cut(s) 219, 321, 399
BciVI GTATCC 2 cut(s) 768, 855
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 3 cut(s) 98, 312, 606
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 4 cut(s) 108, 278, 582, 949
BfmI CTRYAG 1 cut(s) 795
BfuI GTATCC 2 cut(s) 768, 855
BglII AGATCT 3 cut(s) 578, 757, 791
BisI GCNGC 2 cut(s) 275, 395
BlsI GCNGC 2 cut(s) 276, 396
Bme1390I CCNGG 3 cut(s) 219, 321, 399
Bme18I GGWCC 3 cut(s) 215, 316, 742
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 3 cut(s) 215, 316, 742
BmiI GGNNCC 4 cut(s) 318, 340, 393, 743
BmrFI CCNGG 3 cut(s) 219, 321, 399
BmsI GCATC 2 cut(s) 214, 500
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpuEI CTTGAG 1 cut(s) 921
BsaJI CCNNGG 3 cut(s) 218, 312, 319
Bsc4I CCNNNNNNNGG 1 cut(s) 739
Bse118I RCCGGY 1 cut(s) 511
BseBI CCWGG 3 cut(s) 219, 321, 399
BseDI CCNNGG 3 cut(s) 218, 312, 319
BseGI GGATG 2 cut(s) 229, 439
BseLI CCNNNNNNNGG 1 cut(s) 739
BseMII CTCAG 1 cut(s) 864
BseRI GAGGAG 1 cut(s) 906
BseSI GKGCMC 2 cut(s) 454, 631
BseXI GCAGC 1 cut(s) 261
BseYI CCCAGC 1 cut(s) 738
BsgI GTGCAG 1 cut(s) 226
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 1 cut(s) 622
BsiEI CGRYCG 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 302, 755
BslI CCNNNNNNNGG 1 cut(s) 739
BsmAI GTCTC 3 cut(s) 98, 312, 606
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 2 cut(s) 302, 755
BsmI GAATGC 1 cut(s) 906
BsnI GGCC 1 cut(s) 622
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 454, 631
Bsp143I GATC 6 cut(s) 43, 190, 578, 757, 791, 816
BspACI CCGC 4 cut(s) 342, 389, 395, 562
BspANI GGCC 1 cut(s) 622
BspCNI CTCAG 1 cut(s) 865
BspLI GGNNCC 4 cut(s) 318, 340, 393, 743
BsrBI CCGCTC 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 511
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 3 cut(s) 218, 312, 319
BssMI GATC 6 cut(s) 43, 190, 578, 757, 791, 816
BssSI CACGAG 1 cut(s) 447
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 3 cut(s) 219, 321, 399
Bst4CI ACNGT 3 cut(s) 100, 524, 697
BstAPI GCANNNNNTGC 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 1 cut(s) 873
BstF5I GGATG 2 cut(s) 229, 439
BstKTI GATC 6 cut(s) 46, 193, 581, 760, 794, 819
BstMAI GTCTC 3 cut(s) 98, 312, 606
BstMBI GATC 6 cut(s) 43, 190, 578, 757, 791, 816
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 2 cut(s) 178, 365
BstNI CCWGG 3 cut(s) 219, 321, 399
BstSCI CCNGG 3 cut(s) 217, 319, 397
BstSFI CTRYAG 1 cut(s) 795
BstSLI GKGCMC 2 cut(s) 454, 631
BstV1I GCAGC 1 cut(s) 261
BstX2I RGATCY 3 cut(s) 578, 757, 791
BstYI RGATCY 3 cut(s) 578, 757, 791
BsuI GTATCC 2 cut(s) 768, 855
BsuRI GGCC 1 cut(s) 622
BtsCI GGATG 2 cut(s) 229, 439
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 96, 238
Cac8I GCNNGC 1 cut(s) 183
CaiI CAGNNNCTG 1 cut(s) 218
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 3 cut(s) 215, 316, 742
CseI GACGC 2 cut(s) 623, 760
Csp6I GTAC 2 cut(s) 132, 520
CviAII CATG 7 cut(s) 69, 333, 363, 477, 590, 618, 668
CviQI GTAC 2 cut(s) 132, 520
DdeI CTNAG 1 cut(s) 873
DpnI GATC 6 cut(s) 45, 192, 580, 759, 793, 818
DpnII GATC 6 cut(s) 43, 190, 578, 757, 791, 816
EaeI YGGCCR 1 cut(s) 620
Eco47I GGWCC 3 cut(s) 215, 316, 742
Eco57I CTGAAG 3 cut(s) 72, 152, 728
Eco88I CYCGRG 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 217, 319, 397
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 7 cut(s) 72, 336, 366, 480, 593, 621, 671
FaqI GGGAC 2 cut(s) 302, 755
FatI CATG 7 cut(s) 68, 332, 362, 476, 589, 617, 667
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 275, 395
FokI GGATG 2 cut(s) 236, 446
Fsp4HI GCNGC 2 cut(s) 275, 395
FspBI CTAG 4 cut(s) 108, 278, 582, 949
GluI GCNGC 2 cut(s) 275, 395
GsaI CCCAGC 1 cut(s) 742
HaeIII GGCC 1 cut(s) 622
HapII CCGG 1 cut(s) 512
HgaI GACGC 2 cut(s) 623, 760
Hin1II CATG 7 cut(s) 72, 336, 366, 480, 593, 621, 671
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 3 cut(s) 305, 531, 548
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 692
Hpy166II GTNNAC 2 cut(s) 421, 520
Hpy188I TCNGA 3 cut(s) 304, 414, 722
Hpy188III TCNNGA 5 cut(s) 38, 207, 582, 706, 938
Hpy8I GTNNAC 2 cut(s) 421, 520
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 2 cut(s) 538, 858
HpyCH4III ACNGT 3 cut(s) 100, 524, 697
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 2 cut(s) 178, 365
HpyF3I CTNAG 1 cut(s) 873
HpySE526I ACGT 1 cut(s) 645
Hsp92II CATG 7 cut(s) 72, 336, 366, 480, 593, 621, 671
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 6 cut(s) 43, 190, 578, 757, 791, 816
LmnI GCTCC 3 cut(s) 386, 391, 934
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 2 cut(s) 214, 500
MaeI CTAG 4 cut(s) 108, 278, 582, 949
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 2 cut(s) 110, 680
MalI GATC 6 cut(s) 45, 192, 580, 759, 793, 818
MbiI CCGCTC 1 cut(s) 389
MboI GATC 6 cut(s) 43, 190, 578, 757, 791, 816
MboII GAAGA 5 cut(s) 26, 65, 158, 241, 244
MflI RGATCY 3 cut(s) 578, 757, 791
MhlI GDGCHC 2 cut(s) 454, 631
MlsI TGGCCA 1 cut(s) 622
MluCI AATT 2 cut(s) 64, 194
MluNI TGGCCA 1 cut(s) 622
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 2 cut(s) 138, 400
Mox20I TGGCCA 1 cut(s) 622
MscI TGGCCA 1 cut(s) 622
MseI TTAA 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 622
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 3 cut(s) 219, 321, 399
Mva1269I GAATGC 1 cut(s) 906
MvaI CCWGG 3 cut(s) 219, 321, 399
MwoI GCNNNNNNNGC 2 cut(s) 178, 365
NdeII GATC 6 cut(s) 43, 190, 578, 757, 791, 816
NlaIII CATG 7 cut(s) 72, 336, 366, 480, 593, 621, 671
NlaIV GGNNCC 4 cut(s) 318, 340, 393, 743
NmuCI GTSAC 1 cut(s) 680
PctI GAATGC 1 cut(s) 906
PfeI GAWTC 2 cut(s) 531, 548
PkrI GCNGC 2 cut(s) 276, 396
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 3 cut(s) 217, 319, 397
PspFI CCCAGC 1 cut(s) 738
PspGI CCWGG 3 cut(s) 217, 319, 397
PspN4I GGNNCC 4 cut(s) 318, 340, 393, 743
PspPI GGNCC 3 cut(s) 215, 316, 742
PspPPI RGGWCCY 1 cut(s) 215
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 578, 757, 791
RsaI GTAC 2 cut(s) 133, 521
RsaNI GTAC 2 cut(s) 132, 520
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 275, 395
Sau3AI GATC 6 cut(s) 43, 190, 578, 757, 791, 816
Sau96I GGNCC 3 cut(s) 215, 316, 742
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 219, 321, 399
SduI GDGCHC 2 cut(s) 454, 631
SfaNI GCATC 2 cut(s) 214, 500
SfcI CTRYAG 1 cut(s) 795
SinI GGWCC 3 cut(s) 215, 316, 742
SmlI CTYRAG 1 cut(s) 936
SmoI CTYRAG 1 cut(s) 936
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 2 cut(s) 64, 194
SsiI CCGC 4 cut(s) 342, 389, 395, 562
SspMI CTAG 4 cut(s) 108, 278, 582, 949
StyD4I CCNGG 3 cut(s) 217, 319, 397
TaaI ACNGT 3 cut(s) 100, 524, 697
TaiI ACGT 1 cut(s) 648
TaqI TCGA 1 cut(s) 699
TaqII GACCGA 2 cut(s) 304, 759
TasI AATT 2 cut(s) 64, 194
TatI WGTACW 1 cut(s) 519
TauI GCSGC 1 cut(s) 397
TfiI GAWTC 2 cut(s) 531, 548
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TscAI CASTG 2 cut(s) 103, 245
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 4 cut(s) 57, 249, 450, 840
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 2 cut(s) 103, 245
VpaK11BI GGWCC 3 cut(s) 215, 316, 742
XbaI TCTAGA 1 cut(s) 581
XspI CTAG 4 cut(s) 108, 278, 582, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.