MD02G1007400.v1.1

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010359
Physical Location & Seq
Reverse (-)
476550 .. 479497
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1007400.v1.1.491

Sequence Viewer

Length: 885 bp
ATGAGTGTTTCTTTGACAGTGATGGCCTTCAATCTTCACGAAGATCAGACGGAAGACAGTCCCAACTCGTGGGATAAGAGAAAGGATTTATGCATAAGCGTCATAACTAGCTATTCTCCTATGATTCTGTGTACCCAGCAAGGAGTTAAATCACAGTTGGATTATCTTCAGCAGTGCTTGCCAGGTTATGATCAATTTGGAATATCAAGAAAAGGGCCTGAAGACACTGCTGATGAACATTGCACCATCTTCTATGACAAGGAGAAGGTGGAGATGCTTGAAGGTGGAACATTTTGGTTGTCAGAGTCACCTTCTGTTCCCGGAAGCATGTCATGGGGTTCTGAAGTTCCATGTATAGCAACATGGATAGTAAATACAAACATGGATGAGTTCAGTGCTCGTGCTCGTCGACGAAGTGCTTTGCTCACATGGCAGCACATAGCGTCCTTACCACCTGGCTTGCCAGTTGTATATTGTGGAGGTTTCAACACACAAAAGGAATCAACTACTGGCCGGTTTCTTTTGGGGAGATCAAGAGAGCACGGTGCAGTGGGGGATATGAGGGATGCATGGCCTAATGCTCGTGTGAGGAAAAATGCCTCTCTCATTCGCACTTTCCATGGATTTAAAGGTGACAAACAAGGAGCTCTTGAATTCCTCAAGTTGATTTTCAGAGCGCTCTGCCTCTGCTGGGATCGACAAACACAGGATCTACATGTAGATTGGATTCTTTTTAGAGGTAGATCTCTTATCCCTGTCTTGTGTGAAGTGGTGAGTGATAACATTGATGGATATTACCCATCCTCGCACTATCCCATATTTGCCGAGTTTATGCTTCCTCGTACGGTGAGAATGATTGACCCGCCTGCTACGCAGGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

295

Amino Acids

33.34

Weight (kDa)

5.83

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 260 1.8e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 69
AccI GTMKAC 1 cut(s) 409
AciI CCGC 1 cut(s) 863
AclWI GGATC 2 cut(s) 702, 717
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 3 cut(s) 152, 240, 363
AfaI GTAC 2 cut(s) 133, 844
AfeI AGCGCT 1 cut(s) 678
AfiI CCNNNNNNNGG 2 cut(s) 69, 691
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 4 cut(s) 31, 281, 487, 653
AjnI CCWGG 2 cut(s) 181, 454
AluBI AGCT 2 cut(s) 111, 647
AluI AGCT 2 cut(s) 111, 647
Alw21I GWGCWC 4 cut(s) 400, 406, 543, 649
AlwI GGATC 2 cut(s) 702, 717
Aor51HI AGCGCT 1 cut(s) 678
AoxI GGCC 4 cut(s) 24, 215, 511, 572
ApeKI GCWGC 1 cut(s) 433
ApoI RAATTY 1 cut(s) 653
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 4 cut(s) 300, 644, 784, 859
BanII GRGCYC 1 cut(s) 649
BauI CACGAG 3 cut(s) 67, 399, 582
BbsI GAAGAC 2 cut(s) 60, 228
Bbv12I GWGCWC 4 cut(s) 400, 406, 543, 649
BbvI GCAGC 1 cut(s) 445
BccI CCATC 4 cut(s) 16, 254, 782, 808
BciT130I CCWGG 2 cut(s) 183, 456
BclI TGATCA 1 cut(s) 190
BcnI CCSGG 1 cut(s) 321
BfaI CTAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 680
BglII AGATCT 1 cut(s) 743
BisI GCNGC 1 cut(s) 434
BlsI GCNGC 1 cut(s) 435
Bme1390I CCNGG 3 cut(s) 183, 321, 456
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 3 cut(s) 183, 321, 456
BmsI GCATC 2 cut(s) 264, 556
BpiI GAAGAC 2 cut(s) 60, 228
BpuEI CTTGAG 1 cut(s) 644
BpuMI CCSGG 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 619
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 691
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 2 cut(s) 464, 514
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 2 cut(s) 183, 456
BseDI CCNNGG 1 cut(s) 619
BseGI GGATG 3 cut(s) 391, 571, 800
BseLI CCNNNNNNNGG 2 cut(s) 69, 691
BseMI GCAATG 1 cut(s) 238
BseNI ACTGG 2 cut(s) 464, 514
BseXI GCAGC 1 cut(s) 445
BseYI CCCAGC 2 cut(s) 135, 690
BsgI GTGCAG 1 cut(s) 567
BshFI GGCC 4 cut(s) 26, 217, 513, 574
BsiHKAI GWGCWC 4 cut(s) 400, 406, 543, 649
BsiSI CCGG 2 cut(s) 321, 514
BsiWI CGTACG 1 cut(s) 842
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 69, 691
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 4 cut(s) 26, 217, 513, 574
Bsp1286I GDGCHC 4 cut(s) 400, 406, 543, 649
Bsp143I GATC 6 cut(s) 43, 190, 530, 694, 709, 743
Bsp19I CCATGG 1 cut(s) 619
BspACI CCGC 1 cut(s) 863
BspANI GGCC 4 cut(s) 26, 217, 513, 574
BspPI GGATC 2 cut(s) 702, 717
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 513
BsrI ACTGG 2 cut(s) 464, 514
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 619
BssMI GATC 6 cut(s) 43, 190, 530, 694, 709, 743
BssSI CACGAG 3 cut(s) 67, 399, 582
BssT1I CCWWGG 1 cut(s) 619
Bst2BI CACGAG 3 cut(s) 67, 399, 582
Bst2UI CCWGG 2 cut(s) 183, 456
Bst4CI ACNGT 5 cut(s) 19, 59, 156, 545, 847
BstAPI GCANNNNNTGC 1 cut(s) 178
BstC8I GCNNGC 3 cut(s) 179, 461, 867
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 3 cut(s) 391, 571, 800
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 6 cut(s) 46, 193, 533, 697, 712, 746
BstMBI GATC 6 cut(s) 43, 190, 530, 694, 709, 743
BstMWI GCNNNNNNNGC 3 cut(s) 178, 430, 871
BstNI CCWGG 2 cut(s) 183, 456
BstNSI RCATGY 2 cut(s) 331, 719
BstSCI CCNGG 3 cut(s) 181, 319, 454
BstV1I GCAGC 1 cut(s) 445
BstV2I GAAGAC 2 cut(s) 60, 228
BstX2I RGATCY 2 cut(s) 709, 743
BstYI RGATCY 2 cut(s) 709, 743
BsuRI GGCC 4 cut(s) 26, 217, 513, 574
BtgI CCRYGG 1 cut(s) 619
BtsCI GGATG 3 cut(s) 391, 571, 800
BtsI GCAGTG 3 cut(s) 179, 225, 555
BtsIMutI CAGTG 5 cut(s) 24, 179, 225, 400, 555
Cac8I GCNNGC 3 cut(s) 179, 461, 867
CfoI GCGC 1 cut(s) 679
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 2 cut(s) 88, 432
Csp6I GTAC 2 cut(s) 132, 843
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 9 cut(s) 328, 333, 351, 363, 382, 429, 570, 620, 716
CviJI RGCY 7 cut(s) 26, 111, 217, 459, 513, 574, 647
CviKI_1 RGCY 7 cut(s) 26, 111, 217, 459, 513, 574, 647
CviQI GTAC 2 cut(s) 132, 843
DpnI GATC 6 cut(s) 45, 192, 532, 696, 711, 745
DpnII GATC 6 cut(s) 43, 190, 530, 694, 709, 743
DraI TTTAAA 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 649
Eco47III AGCGCT 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 3 cut(s) 152, 240, 363
EcoICRI GAGCTC 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 181, 454
EcoT14I CCWWGG 1 cut(s) 619
EcoT22I ATGCAT 2 cut(s) 95, 571
EcoT38I GRGCYC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 619
FaeI CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 1 cut(s) 45
FatI CATG 9 cut(s) 327, 332, 350, 362, 381, 428, 569, 619, 715
FauI CCCGC 1 cut(s) 870
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 434
FokI GGATG 3 cut(s) 398, 578, 787
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 1 cut(s) 434
FspBI CTAG 1 cut(s) 108
GlaI GCGC 1 cut(s) 678
GluI GCNGC 1 cut(s) 434
GsaI CCCAGC 2 cut(s) 139, 694
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 4 cut(s) 26, 217, 513, 574
HapII CCGG 2 cut(s) 321, 514
HgaI GACGC 2 cut(s) 88, 432
HhaI GCGC 1 cut(s) 679
Hin1II CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 1 cut(s) 410
HindII GTYRAC 1 cut(s) 410
HinfI GANTC 4 cut(s) 124, 305, 500, 727
HpaII CCGG 2 cut(s) 321, 514
HphI GGTGA 4 cut(s) 300, 644, 784, 859
Hpy166II GTNNAC 2 cut(s) 132, 410
Hpy188I TCNGA 4 cut(s) 48, 304, 343, 674
Hpy188III TCNNGA 4 cut(s) 38, 207, 534, 650
Hpy8I GTNNAC 2 cut(s) 132, 410
Hpy99I CGWCG 2 cut(s) 411, 414
HpyAV CCTTC 4 cut(s) 37, 259, 275, 321
HpyCH4III ACNGT 5 cut(s) 19, 59, 156, 545, 847
HpyCH4V TGCA 4 cut(s) 93, 243, 548, 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 430, 871
Hsp92II CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
HspAI GCGC 1 cut(s) 677
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 6 cut(s) 43, 190, 530, 694, 709, 743
LmnI GCTCC 1 cut(s) 644
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 2 cut(s) 264, 556
MaeI CTAG 1 cut(s) 108
MaeIII GTNAC 2 cut(s) 306, 632
MalI GATC 6 cut(s) 45, 192, 532, 696, 711, 745
MboI GATC 6 cut(s) 43, 190, 530, 694, 709, 743
MboII GAAGA 6 cut(s) 26, 53, 65, 158, 233, 241
MflI RGATCY 2 cut(s) 709, 743
MhlI GDGCHC 4 cut(s) 400, 406, 543, 649
MluCI AATT 2 cut(s) 194, 653
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 9 cut(s) 473, 555, 582, 610, 668, 695, 731, 814, 849
Mph1103I ATGCAT 2 cut(s) 95, 571
MseI TTAA 2 cut(s) 147, 627
MspI CCGG 2 cut(s) 321, 514
MspR9I CCNGG 3 cut(s) 183, 321, 456
MvaI CCWGG 2 cut(s) 183, 456
MwoI GCNNNNNNNGC 3 cut(s) 178, 430, 871
NciI CCSGG 1 cut(s) 321
NcoI CCATGG 1 cut(s) 619
NdeII GATC 6 cut(s) 43, 190, 530, 694, 709, 743
NlaIII CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 2 cut(s) 306, 632
NsiI ATGCAT 2 cut(s) 95, 571
NspI RCATGY 2 cut(s) 331, 719
PciI ACATGT 1 cut(s) 715
PcsI WCGNNNNNNNCGW 1 cut(s) 406
PfeI GAWTC 3 cut(s) 124, 500, 727
Pfl23II CGTACG 1 cut(s) 842
PflMI CCANNNNNTGG 1 cut(s) 69
PfoI TCCNGGA 1 cut(s) 319
PkrI GCNGC 1 cut(s) 435
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PscI ACATGT 1 cut(s) 715
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 2 cut(s) 181, 454
PspFI CCCAGC 2 cut(s) 135, 690
PspGI CCWGG 2 cut(s) 181, 454
PspLI CGTACG 1 cut(s) 842
PspPI GGNCC 1 cut(s) 215
PsuI RGATCY 2 cut(s) 709, 743
RsaI GTAC 2 cut(s) 133, 844
RsaNI GTAC 2 cut(s) 132, 843
SacI GAGCTC 1 cut(s) 649
SalI GTCGAC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 147, 627
SatI GCNGC 1 cut(s) 434
Sau3AI GATC 6 cut(s) 43, 190, 530, 694, 709, 743
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 183, 321, 456
SduI GDGCHC 4 cut(s) 400, 406, 543, 649
SfaNI GCATC 2 cut(s) 264, 556
SgrDI CGTCGACG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
Sse9I AATT 2 cut(s) 194, 653
SsiI CCGC 1 cut(s) 863
SspMI CTAG 1 cut(s) 108
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 3 cut(s) 181, 319, 454
StyI CCWWGG 1 cut(s) 619
TaaI ACNGT 5 cut(s) 19, 59, 156, 545, 847
TaqI TCGA 2 cut(s) 409, 697
TasI AATT 2 cut(s) 194, 653
TfiI GAWTC 3 cut(s) 124, 500, 727
Tru1I TTAA 2 cut(s) 147, 627
Tru9I TTAA 2 cut(s) 147, 627
TscAI CASTG 5 cut(s) 24, 179, 232, 400, 555
TseFI GTSAC 2 cut(s) 306, 632
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 2 cut(s) 306, 632
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 5 cut(s) 24, 179, 232, 400, 555
Van91I CCANNNNNTGG 1 cut(s) 69
XapI RAATTY 1 cut(s) 653
XceI RCATGY 2 cut(s) 331, 719
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 1 cut(s) 108
Zsp2I ATGCAT 2 cut(s) 95, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.