RLG00000015706

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010359
Physical Location & Seq
Reverse (-)
857677 .. 859544
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015706

Sequence Viewer

Length: 885 bp
ATGAGTGTTTATTTGACTGTGATGACGTTCAATCTTCATGAAGATCCGACAGCAGATAGTTCTAATTCATGGGAAAAGAGAAGAGATTTGTGTATAAGCGTCGTTACTAGTTATTCTCCGATGATTCTCTGTACTCAACAAGGAGCTAAATCACAATTGGATTATCTTCAACAATGCTTGCCAGGTTATGACCAATTTGGGATATCAAGAAAAGGACCTGAAGACACTTCAGACGAACATTGCACCATCTTCTATGACAAGGAGAAGGTGGAGGTTATTGAAGGTGGAACATTTTGGTTGTCAGAGTCACCTTCTGTCCCTGGAAGCATGTCGTGGGGTGCTCTAGTTCCATGTATTGCTACATGGATAGTAAATACAAACATGGATGAGTTCAGTCCTCGTGCACGGAGGCGAAGTGCTTTGCTTACATGGCAGCACATTGCATCCTTACCACCTAGCTTGCCAGTTGTTTATTGTGGAGGGTTCAACACACAAAAGGAATCGACTACTGGTCGTTTTCTTCTTGGGAGATCAAGAGAGCATGGTGTGGTCGGTGATATGAGGGATACATGGCCTAATGCCCGTGTGAGGAAAAATGCTTCTCTAATTCGCACTTTTCATGGATTTAAAGGTAACAAACAAGGAGCTCTTGAATTCTTCAAGTTGGTTTTCAGAGCCCTGTGCCTTTGCTGGGATCGCCAAACACAGGATCTACACATAGATTGGATTCTTTTCAGAGGTAGATCTCTTGTTCCTGTTTCGTGTGAAGTGGTGAGTGATAACATTGATGGACAATATCCATCCTCCCACTATCCAATATTTGCTGAGTTTCTGCTGCCTCGTGCTGTGAGAATGGTTGACCCACCTGCTCAAGAGGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

295

Amino Acids

33.34

Weight (kDa)

6.18

Isoelectric Point (pI)

58.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 261 1.4e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 874
Acc36I ACCTGC 1 cut(s) 874
AclWI GGATC 3 cut(s) 38, 702, 717
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 2 cut(s) 213, 240
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 588, 691, 706
AgsI TTSAA 6 cut(s) 31, 170, 281, 487, 653, 661
AhdI GACNNNNNGTC 1 cut(s) 510
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 2 cut(s) 181, 319
AluBI AGCT 3 cut(s) 146, 459, 647
AluI AGCT 3 cut(s) 146, 459, 647
Alw21I GWGCWC 3 cut(s) 343, 406, 649
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 3 cut(s) 38, 702, 717
AoxI GGCC 1 cut(s) 572
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 2 cut(s) 433, 835
ApoI RAATTY 1 cut(s) 653
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 3 cut(s) 300, 566, 784
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 406
BanII GRGCYC 2 cut(s) 649, 679
BauI CACGAG 2 cut(s) 399, 840
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 343, 406, 649
BbvI GCAGC 2 cut(s) 445, 822
BccI CCATC 3 cut(s) 254, 782, 808
BciT130I CCWGG 2 cut(s) 183, 321
BciVI GTATCC 1 cut(s) 559
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 108, 344, 456
BfuAI ACCTGC 1 cut(s) 874
BfuI GTATCC 1 cut(s) 559
BglII AGATCT 1 cut(s) 743
BisI GCNGC 2 cut(s) 434, 836
BlsI GCNGC 2 cut(s) 435, 837
Bme1390I CCNGG 2 cut(s) 183, 321
Bme18I GGWCC 1 cut(s) 215
BmeRI GACNNNNNGTC 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 2 cut(s) 183, 321
BmsI GCATC 1 cut(s) 452
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 855
BsaJI CCNNGG 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 3 cut(s) 588, 691, 706
Bse1I ACTGG 2 cut(s) 464, 514
Bse3DI GCAATG 2 cut(s) 238, 438
BseBI CCWGG 2 cut(s) 183, 321
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 3 cut(s) 391, 443, 800
BseLI CCNNNNNNNGG 3 cut(s) 588, 691, 706
BseMI GCAATG 2 cut(s) 238, 438
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 2 cut(s) 464, 514
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 2 cut(s) 445, 822
BseYI CCCAGC 1 cut(s) 690
BshFI GGCC 1 cut(s) 574
BsiHKAI GWGCWC 3 cut(s) 343, 406, 649
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 3 cut(s) 588, 691, 706
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 1 cut(s) 574
Bsp1286I GDGCHC 4 cut(s) 343, 406, 649, 679
Bsp143I GATC 5 cut(s) 43, 530, 694, 709, 743
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 1 cut(s) 817
BspHI TCATGA 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 3 cut(s) 38, 702, 717
BsrDI GCAATG 2 cut(s) 238, 438
BsrI ACTGG 2 cut(s) 464, 514
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 5 cut(s) 43, 530, 694, 709, 743
BssSI CACGAG 2 cut(s) 399, 840
Bst2BI CACGAG 2 cut(s) 399, 840
Bst2UI CCWGG 2 cut(s) 183, 321
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 76
BstC8I GCNNGC 2 cut(s) 179, 461
BstDEI CTNAG 1 cut(s) 825
BstF5I GGATG 3 cut(s) 391, 443, 800
BstKTI GATC 5 cut(s) 46, 533, 697, 712, 746
BstMBI GATC 5 cut(s) 43, 530, 694, 709, 743
BstMWI GCNNNNNNNGC 2 cut(s) 430, 696
BstNI CCWGG 2 cut(s) 183, 321
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 181, 319
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 2 cut(s) 445, 822
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 3 cut(s) 43, 709, 743
BstYI RGATCY 3 cut(s) 43, 709, 743
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 1 cut(s) 574
BtsCI GGATG 3 cut(s) 391, 443, 800
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 2 cut(s) 179, 461
CciI TCATGA 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviJI RGCY 5 cut(s) 146, 459, 574, 647, 677
CviKI_1 RGCY 5 cut(s) 146, 459, 574, 647, 677
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 825
DpnI GATC 5 cut(s) 45, 532, 696, 711, 745
DpnII GATC 5 cut(s) 43, 530, 694, 709, 743
DraI TTTAAA 1 cut(s) 628
DriI GACNNNNNGTC 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 76
Eam1105I GACNNNNNGTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 647
Eco24I GRGCYC 2 cut(s) 649, 679
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 2 cut(s) 213, 240
EcoICRI GAGCTC 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 181, 319
EcoRV GATATC 1 cut(s) 204
EcoT38I GRGCYC 2 cut(s) 649, 679
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 434, 836
FokI GGATG 3 cut(s) 398, 430, 787
FriOI GRGCYC 2 cut(s) 649, 679
Fsp4HI GCNGC 2 cut(s) 434, 836
FspBI CTAG 3 cut(s) 108, 344, 456
GluI GCNGC 2 cut(s) 434, 836
GsaI CCCAGC 1 cut(s) 694
HaeIII GGCC 1 cut(s) 574
HgaI GACGC 1 cut(s) 88
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 4 cut(s) 124, 305, 500, 727
HphI GGTGA 3 cut(s) 300, 566, 784
Hpy166II GTNNAC 2 cut(s) 404, 859
Hpy188I TCNGA 6 cut(s) 48, 120, 232, 304, 674, 737
Hpy188III TCNNGA 5 cut(s) 38, 207, 534, 650, 872
Hpy8I GTNNAC 2 cut(s) 404, 859
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 259, 275, 321
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 243, 404, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 430, 696
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 1 cut(s) 26
Kzo9I GATC 5 cut(s) 43, 530, 694, 709, 743
LmnI GCTCC 2 cut(s) 143, 644
Lsp1109I GCAGC 2 cut(s) 445, 822
LweI GCATC 1 cut(s) 452
MaeI CTAG 3 cut(s) 108, 344, 456
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 3 cut(s) 103, 306, 632
MalI GATC 5 cut(s) 45, 532, 696, 711, 745
MboI GATC 5 cut(s) 43, 530, 694, 709, 743
MboII GAAGA 8 cut(s) 26, 53, 93, 158, 233, 241, 512, 649
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 3 cut(s) 43, 709, 743
MhlI GDGCHC 4 cut(s) 343, 406, 649, 679
MluCI AATT 6 cut(s) 64, 155, 194, 606, 653, 880
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 71
MseI TTAA 1 cut(s) 627
MspR9I CCNGG 2 cut(s) 183, 321
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 2 cut(s) 183, 321
MwoI GCNNNNNNNGC 2 cut(s) 430, 696
NdeII GATC 5 cut(s) 43, 530, 694, 709, 743
NmuCI GTSAC 1 cut(s) 306
NspI RCATGY 1 cut(s) 331
PagI TCATGA 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 874
PfeI GAWTC 3 cut(s) 124, 500, 727
PkrI GCNGC 2 cut(s) 435, 837
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 649
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 181, 319
PspFI CCCAGC 1 cut(s) 690
PspGI CCWGG 2 cut(s) 181, 319
PspPI GGNCC 1 cut(s) 215
PspPPI RGGWCCY 1 cut(s) 215
PsuI RGATCY 3 cut(s) 43, 709, 743
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SacI GAGCTC 1 cut(s) 649
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 2 cut(s) 434, 836
Sau3AI GATC 5 cut(s) 43, 530, 694, 709, 743
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 183, 321
SduI GDGCHC 4 cut(s) 343, 406, 649, 679
SfaNI GCATC 1 cut(s) 452
SinI GGWCC 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 6 cut(s) 64, 155, 194, 606, 653, 880
SspI AATATT 1 cut(s) 819
SspMI CTAG 3 cut(s) 108, 344, 456
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 2 cut(s) 181, 319
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 29
TaqI TCGA 1 cut(s) 503
TasI AATT 6 cut(s) 64, 155, 194, 606, 653, 880
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 3 cut(s) 124, 500, 727
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 2 cut(s) 433, 835
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 4 cut(s) 26, 54, 57, 608
TspGWI ACGGA 1 cut(s) 421
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 653
XceI RCATGY 1 cut(s) 331
XspI CTAG 3 cut(s) 108, 344, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.