Rroxscaffold_2G00155180

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010359
Physical Location & Seq
Forward (+)
91583350 .. 91585717
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155180.1

Sequence Viewer

Length: 933 bp
ATGAGTGTTTCTTTGACTGTGATGACGTTCAATCTTCATGAAGATCCGACAGCAGATAGTTCTAATTCATGGGAAAAGAGAAGAGATTTGTGTATAAGCGTCGTTACTAGTTATTCTCCGATGATTCTCTGTACTCAACAAGGAGCTAAATCACAATTGGATTATCTTCAACAATGTTTGCCAGGTTATGGCCAATTTGGGATATCAAGAAAAGGACCTGAAGACACTTCAGACGAACATTGCACCATCTTCTATGACAAGGAGAAGGTGGAGGTTATTGAAGGTGGAACATTTTGGTTGTCAGAGTCACCTTCTGTCCCTGGAAGCATGTCGTGGGGTGCTCTAGTTCCATGTATTGCTACATGGGTGACATTTCAACTGAAAGGAGTTGAGCCCCCGGGCTTTTCATTCCAGATAGTAAATACAAACATGGATGAGTTCAGTCCTCGTGCACGGAGGCGAAGTGCTTTGCTTACATGGCAGCACATTGCATCCTTACCACCTAGCTTGCCAGTTGTTTATTGTGGAGGGTTCAACACACAAAAGGAATCAACTACTGGTCGTTTTCTTCTTGGGAGATCAAGAGAGCATGGTGTGGTTGGTGATATGAGGGATACATGGCCTAATGCCCGTGTGAGGAAAAATGCTTCTCTAATTCGCACTTTTCATGGATTTAAAGGTAACAAACAAGGAGCTCTTGAATTCTTCAAGTTGGTTTTCAGAGCCCTGTGCCTTTGCTGGGATCGCCAAACACAGGATCTACACATAGATTGGATTCTTTTCAGAGGTAGATCTCTTGTTCCTGTTTCATGTGAAGTGGTGAGTGATAATATTGATGGACAATATCCATCCTCCCACTATCCAATATTTGCTGAGTTTCTGCTTCCTCGTGCTGTGAGAATGGTTGACCCACCTGCTCAAGAGGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

310

Amino Acids

34.97

Weight (kDa)

6.39

Isoelectric Point (pI)

55.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 277 2.8e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 922
Acc36I ACCTGC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 188
AclWI GGATC 3 cut(s) 38, 750, 765
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 701
AcuI CTGAAG 2 cut(s) 213, 240
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 4 cut(s) 188, 636, 739, 754
AgsI TTSAA 7 cut(s) 31, 170, 281, 377, 535, 701, 709
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 2 cut(s) 181, 319
AluBI AGCT 3 cut(s) 146, 507, 695
AluI AGCT 3 cut(s) 146, 507, 695
Alw21I GWGCWC 3 cut(s) 343, 454, 697
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 3 cut(s) 38, 750, 765
Ama87I CYCGRG 1 cut(s) 397
AoxI GGCC 2 cut(s) 190, 620
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 1 cut(s) 481
ApoI RAATTY 1 cut(s) 701
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 2 cut(s) 398, 399
AsuHPI GGTGA 4 cut(s) 300, 379, 614, 832
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 454
BalI TGGCCA 1 cut(s) 192
BanII GRGCYC 3 cut(s) 396, 697, 727
BauI CACGAG 2 cut(s) 447, 888
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 343, 454, 697
BbvI GCAGC 1 cut(s) 493
BccI CCATC 3 cut(s) 254, 830, 856
BciT130I CCWGG 2 cut(s) 183, 321
BciVI GTATCC 1 cut(s) 607
BcnI CCSGG 2 cut(s) 398, 399
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 108, 344, 504
BfuAI ACCTGC 1 cut(s) 922
BfuI GTATCC 1 cut(s) 607
BglII AGATCT 1 cut(s) 791
BisI GCNGC 1 cut(s) 482
BlsI GCNGC 1 cut(s) 483
Bme1390I CCNGG 4 cut(s) 183, 321, 398, 399
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 4 cut(s) 183, 321, 398, 399
BmsI GCATC 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 903
BpuMI CCSGG 2 cut(s) 398, 399
BsaJI CCNNGG 3 cut(s) 319, 396, 397
BsaXI ACNNNNNCTCC 2 cut(s) 448, 478
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 636, 739, 754
Bse1I ACTGG 2 cut(s) 512, 562
Bse3DI GCAATG 2 cut(s) 238, 486
BseBI CCWGG 2 cut(s) 183, 321
BseDI CCNNGG 3 cut(s) 319, 396, 397
BseGI GGATG 3 cut(s) 439, 491, 848
BseLI CCNNNNNNNGG 4 cut(s) 188, 636, 739, 754
BseMI GCAATG 2 cut(s) 238, 486
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 2 cut(s) 512, 562
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 1 cut(s) 493
BseYI CCCAGC 1 cut(s) 738
BshFI GGCC 2 cut(s) 192, 622
BsiHKAI GWGCWC 3 cut(s) 343, 454, 697
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 1 cut(s) 398
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 4 cut(s) 188, 636, 739, 754
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 2 cut(s) 192, 622
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 5 cut(s) 343, 396, 454, 697, 727
Bsp143I GATC 5 cut(s) 43, 578, 742, 757, 791
BspANI GGCC 2 cut(s) 192, 622
BspCNI CTCAG 1 cut(s) 865
BspHI TCATGA 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 3 cut(s) 38, 750, 765
BsrDI GCAATG 2 cut(s) 238, 486
BsrI ACTGG 2 cut(s) 512, 562
BssECI CCNNGG 3 cut(s) 319, 396, 397
BssMI GATC 5 cut(s) 43, 578, 742, 757, 791
BssSI CACGAG 2 cut(s) 447, 888
Bst2BI CACGAG 2 cut(s) 447, 888
Bst2UI CCWGG 2 cut(s) 183, 321
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 509
BstDEI CTNAG 1 cut(s) 873
BstF5I GGATG 3 cut(s) 439, 491, 848
BstKTI GATC 5 cut(s) 46, 581, 745, 760, 794
BstMBI GATC 5 cut(s) 43, 578, 742, 757, 791
BstMWI GCNNNNNNNGC 2 cut(s) 478, 744
BstNI CCWGG 2 cut(s) 183, 321
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 4 cut(s) 181, 319, 396, 397
BstSLI GKGCMC 1 cut(s) 454
BstV1I GCAGC 1 cut(s) 493
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 3 cut(s) 43, 757, 791
BstYI RGATCY 3 cut(s) 43, 757, 791
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 2 cut(s) 192, 622
BtsCI GGATG 3 cut(s) 439, 491, 848
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 1 cut(s) 509
CciI TCATGA 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 215
Cfr9I CCCGGG 1 cut(s) 397
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 489, 524
CviJI RGCY 8 cut(s) 146, 192, 394, 402, 507, 622, 695, 725
CviKI_1 RGCY 8 cut(s) 146, 192, 394, 402, 507, 622, 695, 725
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 873
DpnI GATC 5 cut(s) 45, 580, 744, 759, 793
DpnII GATC 5 cut(s) 43, 578, 742, 757, 791
DraI TTTAAA 1 cut(s) 676
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 695
Eco24I GRGCYC 3 cut(s) 396, 697, 727
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 695
Eco57I CTGAAG 2 cut(s) 213, 240
Eco88I CYCGRG 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 2 cut(s) 181, 319
EcoRV GATATC 1 cut(s) 204
EcoT38I GRGCYC 3 cut(s) 396, 697, 727
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FaqI GGGAC 1 cut(s) 302
Fnu4HI GCNGC 1 cut(s) 482
FokI GGATG 3 cut(s) 446, 478, 835
FriOI GRGCYC 3 cut(s) 396, 697, 727
Fsp4HI GCNGC 1 cut(s) 482
FspBI CTAG 3 cut(s) 108, 344, 504
GluI GCNGC 1 cut(s) 482
GsaI CCCAGC 1 cut(s) 742
HaeIII GGCC 2 cut(s) 192, 622
HapII CCGG 1 cut(s) 398
HgaI GACGC 1 cut(s) 88
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HinfI GANTC 4 cut(s) 124, 305, 548, 775
HpaII CCGG 1 cut(s) 398
HphI GGTGA 4 cut(s) 300, 379, 614, 832
Hpy166II GTNNAC 2 cut(s) 452, 907
Hpy188I TCNGA 6 cut(s) 48, 120, 232, 304, 722, 785
Hpy188III TCNNGA 6 cut(s) 38, 207, 412, 582, 698, 920
Hpy8I GTNNAC 2 cut(s) 452, 907
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 259, 275, 321
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 243, 452, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 478, 744
HpyF3I CTNAG 1 cut(s) 873
HpySE526I ACGT 1 cut(s) 26
Kzo9I GATC 5 cut(s) 43, 578, 742, 757, 791
LmnI GCTCC 2 cut(s) 143, 692
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 1 cut(s) 500
MaeI CTAG 3 cut(s) 108, 344, 504
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 4 cut(s) 103, 306, 367, 680
MalI GATC 5 cut(s) 45, 580, 744, 759, 793
MboI GATC 5 cut(s) 43, 578, 742, 757, 791
MboII GAAGA 8 cut(s) 26, 53, 93, 158, 233, 241, 560, 697
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 3 cut(s) 43, 757, 791
MhlI GDGCHC 5 cut(s) 343, 396, 454, 697, 727
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 6 cut(s) 64, 155, 194, 654, 701, 928
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 192
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 4 cut(s) 183, 321, 398, 399
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 2 cut(s) 183, 321
MwoI GCNNNNNNNGC 2 cut(s) 478, 744
NciI CCSGG 2 cut(s) 398, 399
NdeII GATC 5 cut(s) 43, 578, 742, 757, 791
NmuCI GTSAC 2 cut(s) 306, 367
NspI RCATGY 1 cut(s) 331
PagI TCATGA 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 922
PfeI GAWTC 3 cut(s) 124, 548, 775
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 1 cut(s) 483
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 697
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 2 cut(s) 181, 319
PspFI CCCAGC 1 cut(s) 738
PspGI CCWGG 2 cut(s) 181, 319
PspPI GGNCC 1 cut(s) 215
PspPPI RGGWCCY 1 cut(s) 215
PsuI RGATCY 3 cut(s) 43, 757, 791
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SacI GAGCTC 1 cut(s) 697
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 1 cut(s) 482
Sau3AI GATC 5 cut(s) 43, 578, 742, 757, 791
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 4 cut(s) 183, 321, 398, 399
SduI GDGCHC 5 cut(s) 343, 396, 454, 697, 727
SfaNI GCATC 1 cut(s) 500
SinI GGWCC 1 cut(s) 215
SmaI CCCGGG 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 918
SmoI CTYRAG 1 cut(s) 918
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 6 cut(s) 64, 155, 194, 654, 701, 928
SspI AATATT 2 cut(s) 832, 867
SspMI CTAG 3 cut(s) 108, 344, 504
SstI GAGCTC 1 cut(s) 697
StyD4I CCNGG 4 cut(s) 181, 319, 396, 397
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 29
TasI AATT 6 cut(s) 64, 155, 194, 654, 701, 928
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 3 cut(s) 124, 548, 775
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 2 cut(s) 306, 367
TseI GCWGC 1 cut(s) 481
Tsp45I GTSAC 2 cut(s) 306, 367
TspDTI ATGAA 6 cut(s) 26, 54, 57, 396, 656, 798
TspGWI ACGGA 1 cut(s) 469
TspMI CCCGGG 1 cut(s) 397
Van91I CCANNNNNTGG 1 cut(s) 188
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 701
XceI RCATGY 1 cut(s) 331
XmaI CCCGGG 1 cut(s) 397
XspI CTAG 3 cut(s) 108, 344, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.