Rw2G000970

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010359
Physical Location & Seq
Reverse (-)
807125 .. 808995
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000970.1

Sequence Viewer

Length: 945 bp
ATGAGTGTCTATTTGACTGTGATGACGTTCAATCTTCATGAAGATCCGACAGCAGATAGTTCTAATTCATGGGAAAAGAGAAGAGATTTGTGTATAAGCGTCGTTACTAGTTATTCTCCGATGATTCTCTGTACTCAACAAGGAGCTAAATCACAATTGGATTATCTTCAACAATGCTTGCCAGGTTATGACCAATTTGGGATATCAAGAAAAGGACCTGAAGACACTTCAGACGAACATTGCACCATCTTCTATGACAAGGAGAAGGTGGAGGTTATTGAAGGTGGAACATTTTGGTTGTCAGAGTCACCTTCTGTCCCTGGAAGCATGTCGTGGGGTGCTCTAGTTCCATGTATTGCTACATGGGTGATATCCTTGCCATTTTTCTTTCTCCATAATTTTGAGCCCCCTGGCTTTTCATTCCAGATAGTAAATACAAACATGGATGAGTTCAGTCCTCGTGCACGGAGGCGAAGTGCTTTGCTTACATGGCAGCACATTGCATCCTTACCACCTAGCTTGCCAGTTGTTTATTGTGGAGGGTTCAACACACAAAAGGAATCAACTACTGGTCGTTTTCTTCTTGGGAGATCAAGAGAGCATGGTGTGGTCGGTGATATGAGGGATACATGGCCTAATGCCCGTGTGAGGAAAAATGCTTCTCTAATTCGCACTTTTCATGGATTTAAAGGTAACAAACAAGGAGCTCTTGAATTCTTCAAGTTGGTTTTCAGAGCCCTGTGCCTTTGCTGGGATCGCCAAACACAGGATTTACACATAGATTGGATTCTTTTCAGAGGTAGATCTCTTGTTCCTGTTTCGTGTGAAGTGGTGAGTGATCACATTGATGGACAATATCCATCCTCCCACTATCCAATATTTGCTGAGTTTCTGCTTCCTCGTGCTGTGAGAATTGCTGGCCCACCTGCTCAAGAGCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

314

Amino Acids

35.61

Weight (kDa)

6.5

Isoelectric Point (pI)

57.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 281 1.7e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 934
Acc36I ACCTGC 1 cut(s) 934
AclWI GGATC 2 cut(s) 38, 762
AcsI RAATTY 1 cut(s) 713
AcuI CTGAAG 2 cut(s) 213, 240
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 3 cut(s) 648, 751, 766
AgsI TTSAA 6 cut(s) 31, 170, 281, 547, 713, 721
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 3 cut(s) 181, 319, 409
AluBI AGCT 3 cut(s) 146, 519, 707
AluI AGCT 3 cut(s) 146, 519, 707
Alw21I GWGCWC 3 cut(s) 343, 466, 709
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 2 cut(s) 38, 762
AoxI GGCC 2 cut(s) 632, 919
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 1 cut(s) 493
ApoI RAATTY 1 cut(s) 713
AspS9I GGNCC 2 cut(s) 215, 920
AsuHPI GGTGA 4 cut(s) 300, 379, 626, 844
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 466
BanII GRGCYC 3 cut(s) 408, 709, 739
BauI CACGAG 2 cut(s) 459, 900
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 343, 466, 709
BbvI GCAGC 1 cut(s) 505
BccI CCATC 3 cut(s) 254, 842, 868
BciT130I CCWGG 3 cut(s) 183, 321, 411
BciVI GTATCC 1 cut(s) 619
BclI TGATCA 1 cut(s) 838
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 3 cut(s) 108, 344, 516
BfuAI ACCTGC 1 cut(s) 934
BfuI GTATCC 1 cut(s) 619
BglII AGATCT 1 cut(s) 803
BisI GCNGC 1 cut(s) 494
BlsI GCNGC 1 cut(s) 495
Bme1390I CCNGG 3 cut(s) 183, 321, 411
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 215, 920
BmrFI CCNGG 3 cut(s) 183, 321, 411
BmsI GCATC 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 319, 409
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 648, 751, 766
Bse1I ACTGG 2 cut(s) 524, 574
Bse3DI GCAATG 2 cut(s) 238, 498
BseBI CCWGG 3 cut(s) 183, 321, 411
BseDI CCNNGG 2 cut(s) 319, 409
BseGI GGATG 3 cut(s) 451, 503, 860
BseLI CCNNNNNNNGG 3 cut(s) 648, 751, 766
BseMI GCAATG 2 cut(s) 238, 498
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 2 cut(s) 524, 574
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 1 cut(s) 505
BseYI CCCAGC 1 cut(s) 750
BshFI GGCC 2 cut(s) 634, 921
BsiHKAI GWGCWC 3 cut(s) 343, 466, 709
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 3 cut(s) 648, 751, 766
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 2 cut(s) 634, 921
Bsp1286I GDGCHC 5 cut(s) 343, 408, 466, 709, 739
Bsp143I GATC 5 cut(s) 43, 590, 754, 803, 838
BspANI GGCC 2 cut(s) 634, 921
BspCNI CTCAG 1 cut(s) 877
BspHI TCATGA 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 934
BspPI GGATC 2 cut(s) 38, 762
BsrDI GCAATG 2 cut(s) 238, 498
BsrI ACTGG 2 cut(s) 524, 574
BssECI CCNNGG 2 cut(s) 319, 409
BssMI GATC 5 cut(s) 43, 590, 754, 803, 838
BssSI CACGAG 2 cut(s) 459, 900
Bst2BI CACGAG 2 cut(s) 459, 900
Bst2UI CCWGG 3 cut(s) 183, 321, 411
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 76
BstC8I GCNNGC 3 cut(s) 179, 521, 919
BstDEI CTNAG 1 cut(s) 885
BstF5I GGATG 3 cut(s) 451, 503, 860
BstKTI GATC 5 cut(s) 46, 593, 757, 806, 841
BstMBI GATC 5 cut(s) 43, 590, 754, 803, 838
BstMWI GCNNNNNNNGC 2 cut(s) 490, 756
BstNI CCWGG 3 cut(s) 183, 321, 411
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 181, 319, 409
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 1 cut(s) 505
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 2 cut(s) 43, 803
BstYI RGATCY 2 cut(s) 43, 803
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 2 cut(s) 634, 921
BtsCI GGATG 3 cut(s) 451, 503, 860
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 3 cut(s) 179, 521, 919
CciI TCATGA 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 215, 920
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 501, 536
CviJI RGCY 8 cut(s) 146, 406, 414, 519, 634, 707, 737, 921
CviKI_1 RGCY 8 cut(s) 146, 406, 414, 519, 634, 707, 737, 921
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 885
DpnI GATC 5 cut(s) 45, 592, 756, 805, 840
DpnII GATC 5 cut(s) 43, 590, 754, 803, 838
DraI TTTAAA 1 cut(s) 688
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 707
Eco24I GRGCYC 3 cut(s) 408, 709, 739
Eco32I GATATC 2 cut(s) 204, 372
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 707
Eco57I CTGAAG 2 cut(s) 213, 240
EcoICRI GAGCTC 1 cut(s) 707
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 3 cut(s) 181, 319, 409
EcoRV GATATC 2 cut(s) 204, 372
EcoT38I GRGCYC 3 cut(s) 408, 709, 739
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FaqI GGGAC 1 cut(s) 302
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 1 cut(s) 494
FokI GGATG 3 cut(s) 458, 490, 847
FriOI GRGCYC 3 cut(s) 408, 709, 739
Fsp4HI GCNGC 1 cut(s) 494
FspBI CTAG 3 cut(s) 108, 344, 516
GluI GCNGC 1 cut(s) 494
GsaI CCCAGC 1 cut(s) 754
HaeIII GGCC 2 cut(s) 634, 921
HgaI GACGC 1 cut(s) 88
HinfI GANTC 4 cut(s) 124, 305, 560, 787
HphI GGTGA 4 cut(s) 300, 379, 626, 844
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 6 cut(s) 48, 120, 232, 304, 734, 797
Hpy188III TCNNGA 6 cut(s) 38, 207, 424, 594, 710, 932
Hpy8I GTNNAC 1 cut(s) 464
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 259, 275, 321
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 3 cut(s) 243, 464, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 490, 756
HpyF3I CTNAG 1 cut(s) 885
HpySE526I ACGT 1 cut(s) 26
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 5 cut(s) 43, 590, 754, 803, 838
LmnI GCTCC 2 cut(s) 143, 704
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 1 cut(s) 512
MaeI CTAG 3 cut(s) 108, 344, 516
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 3 cut(s) 103, 306, 692
MalI GATC 5 cut(s) 45, 592, 756, 805, 840
MboI GATC 5 cut(s) 43, 590, 754, 803, 838
MboII GAAGA 8 cut(s) 26, 53, 93, 158, 233, 241, 572, 709
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 2 cut(s) 43, 803
MhlI GDGCHC 5 cut(s) 343, 408, 466, 709, 739
MluCI AATT 8 cut(s) 64, 155, 194, 397, 666, 713, 912, 940
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 9 cut(s) 265, 462, 468, 533, 615, 642, 791, 874, 909
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 846
MspR9I CCNGG 3 cut(s) 183, 321, 411
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 3 cut(s) 183, 321, 411
MwoI GCNNNNNNNGC 2 cut(s) 490, 756
NdeII GATC 5 cut(s) 43, 590, 754, 803, 838
NmuCI GTSAC 1 cut(s) 306
NspI RCATGY 1 cut(s) 331
PagI TCATGA 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 934
PfeI GAWTC 3 cut(s) 124, 560, 787
PkrI GCNGC 1 cut(s) 495
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 3 cut(s) 181, 319, 409
PspFI CCCAGC 1 cut(s) 750
PspGI CCWGG 3 cut(s) 181, 319, 409
PspPI GGNCC 2 cut(s) 215, 920
PspPPI RGGWCCY 1 cut(s) 215
PsuI RGATCY 2 cut(s) 43, 803
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 846
SacI GAGCTC 1 cut(s) 709
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 1 cut(s) 494
Sau3AI GATC 5 cut(s) 43, 590, 754, 803, 838
Sau96I GGNCC 2 cut(s) 215, 920
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 183, 321, 411
SduI GDGCHC 5 cut(s) 343, 408, 466, 709, 739
SfaNI GCATC 1 cut(s) 512
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 846
SmlI CTYRAG 1 cut(s) 930
SmoI CTYRAG 1 cut(s) 930
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 8 cut(s) 64, 155, 194, 397, 666, 713, 912, 940
SspI AATATT 1 cut(s) 879
SspMI CTAG 3 cut(s) 108, 344, 516
SstI GAGCTC 1 cut(s) 709
StyD4I CCNGG 3 cut(s) 181, 319, 409
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 29
TasI AATT 8 cut(s) 64, 155, 194, 397, 666, 713, 912, 940
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 3 cut(s) 124, 560, 787
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 1 cut(s) 493
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 5 cut(s) 26, 54, 57, 408, 668
TspGWI ACGGA 1 cut(s) 481
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 1 cut(s) 713
XceI RCATGY 1 cut(s) 331
XspI CTAG 3 cut(s) 108, 344, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.