MD15G1144700.v1.1

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010359
Physical Location & Seq
Reverse (-)
10680880 .. 10684132
3253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1144700.v1.1.491

Sequence Viewer

Length: 885 bp
ATGAGTGTTTCTTTGACAGTGATGACCTTCAATCTTCACGAGGATCAGACGGAGGACAGTCCCTACTCGTGGGATAAGAGAAGGGATTTGTGCATAAGCGTCATTACTAGTTATTCTCCTATCATTCTGTGTACCCAGCAAGGAGTTAAATCACAGTTGGATTATCTTCAGCAGTGCTTGCCAGGTTATGATCAATTTGGAATATCAAGAAAAGGGCCTGAAGACACTTCTGATGAACATTGCACCATCTTCTATGACAAGGAGAAGGTGGAGCTGCTTGAAGGTGGAACGTTTTGGTTGTCAGAGTCACCTTCTGTCCCTGGAAGCATGTCATGGGGTTCTGAAGTTCCATGTATTGCAACATGGATAGTAAATACAAACATGGATGAGTTCAGTCCTCGTGCCCGTCGACGAAGTGCTTTGCTCACATGGCAGCACATAGCATCCTTGCCACCTGGCTTGCCAGTTGTATATTGTGGAGGTTTCAACACACAAAAGGAATCAACTACCGGCCGGTTTCTTTTGGGGAGATCAAGAGAGCACGGTGCAGTGGGGGATATGAGGGATGCATGGCCTAATGCCCGTGTGAGGAAAAATGTCTCTCTTATTCGCACTTTTCATGGATTTAAAGGTGACAAACAAGGAGCTCTTGAATTCCTCAAGTTGGTTTTCAGAGCGCTCTGCCTTTGCTGGGATCGCCAAACACAGGATCTGCATGTAGATTGGATTCTTTTTAGAGGTAGGTCTCTTAGTCCTGTTTTATGTGAAGTGGTGAGTGATAACATTGATGGATATTACCCATCCTCGCACTATCCCATATTTGCCGAGTTTATGCTTCCTCGCACGGTGAGAATGATTGACCCACCTGCTCCCGAGGGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

295

Amino Acids

33.37

Weight (kDa)

5.83

Isoelectric Point (pI)

58.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 8 - 260 1.6e-07 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 874
Acc36I ACCTGC 1 cut(s) 874
AccI GTMKAC 1 cut(s) 409
AclI AACGTT 1 cut(s) 290
AclWI GGATC 3 cut(s) 51, 702, 717
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 3 cut(s) 152, 240, 363
AfaI GTAC 1 cut(s) 133
AfeI AGCGCT 1 cut(s) 678
AfiI CCNNNNNNNGG 5 cut(s) 69, 588, 664, 691, 706
AgsI TTSAA 4 cut(s) 31, 281, 487, 653
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 3 cut(s) 181, 319, 454
AluBI AGCT 2 cut(s) 274, 647
AluI AGCT 2 cut(s) 274, 647
Alw21I GWGCWC 2 cut(s) 543, 649
Alw26I GTCTC 2 cut(s) 604, 750
AlwI GGATC 3 cut(s) 51, 702, 717
AlwNI CAGNNNCTG 1 cut(s) 712
Ama87I CYCGRG 1 cut(s) 872
Aor51HI AGCGCT 1 cut(s) 678
AoxI GGCC 3 cut(s) 215, 511, 572
ApeKI GCWGC 2 cut(s) 274, 433
ApoI RAATTY 1 cut(s) 653
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 300, 644, 784, 859
AvaI CYCGRG 1 cut(s) 872
BaeGI GKGCMC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 649
BauI CACGAG 3 cut(s) 38, 67, 399
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 2 cut(s) 543, 649
BbvI GCAGC 2 cut(s) 261, 445
BccI CCATC 3 cut(s) 254, 782, 808
BciT130I CCWGG 3 cut(s) 183, 321, 456
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 604, 750
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 874
BisI GCNGC 2 cut(s) 275, 434
BlsI GCNGC 2 cut(s) 276, 435
Bme1390I CCNGG 3 cut(s) 183, 321, 456
BmeT110I CYCGRG 1 cut(s) 872
BmgT120I GGNCC 1 cut(s) 215
BmrFI CCNGG 3 cut(s) 183, 321, 456
BmsI GCATC 2 cut(s) 452, 556
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 750
BsaJI CCNNGG 2 cut(s) 319, 873
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 588, 664, 691, 706
Bse118I RCCGGY 2 cut(s) 509, 513
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 3 cut(s) 183, 321, 456
BseDI CCNNGG 2 cut(s) 319, 873
BseGI GGATG 4 cut(s) 391, 443, 571, 800
BseLI CCNNNNNNNGG 5 cut(s) 69, 588, 664, 691, 706
BseMI GCAATG 1 cut(s) 238
BseNI ACTGG 1 cut(s) 464
BseSI GKGCMC 1 cut(s) 406
BseX3I CGGCCG 1 cut(s) 511
BseXI GCAGC 2 cut(s) 261, 445
BseYI CCCAGC 2 cut(s) 135, 690
BsgI GTGCAG 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 514
BshFI GGCC 3 cut(s) 217, 513, 574
BsiEI CGRYCG 1 cut(s) 514
BsiHKAI GWGCWC 2 cut(s) 543, 649
BsiHKCI CYCGRG 1 cut(s) 872
BsiSI CCGG 2 cut(s) 510, 514
BslFI GGGAC 2 cut(s) 45, 302
BslI CCNNNNNNNGG 5 cut(s) 69, 588, 664, 691, 706
BsmAI GTCTC 2 cut(s) 604, 750
BsmFI GGGAC 2 cut(s) 45, 302
BsnI GGCC 3 cut(s) 217, 513, 574
Bso31I GGTCTC 1 cut(s) 750
BsoBI CYCGRG 1 cut(s) 872
Bsp1286I GDGCHC 3 cut(s) 406, 543, 649
Bsp143I GATC 5 cut(s) 43, 190, 530, 694, 709
BspANI GGCC 3 cut(s) 217, 513, 574
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 3 cut(s) 51, 702, 717
BspTNI GGTCTC 1 cut(s) 750
BsrDI GCAATG 1 cut(s) 238
BsrFI RCCGGY 2 cut(s) 509, 513
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 2 cut(s) 509, 513
BssECI CCNNGG 2 cut(s) 319, 873
BssMI GATC 5 cut(s) 43, 190, 530, 694, 709
BssSI CACGAG 3 cut(s) 38, 67, 399
Bst2BI CACGAG 3 cut(s) 38, 67, 399
Bst2UI CCWGG 3 cut(s) 183, 321, 456
Bst4CI ACNGT 5 cut(s) 19, 59, 156, 545, 847
BstAPI GCANNNNNTGC 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 179, 461
BstDEI CTNAG 1 cut(s) 749
BstF5I GGATG 4 cut(s) 391, 443, 571, 800
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 5 cut(s) 46, 193, 533, 697, 712
BstMAI GTCTC 2 cut(s) 604, 750
BstMBI GATC 5 cut(s) 43, 190, 530, 694, 709
BstMCI CGRYCG 1 cut(s) 514
BstMWI GCNNNNNNNGC 3 cut(s) 178, 430, 696
BstNI CCWGG 3 cut(s) 183, 321, 456
BstNSI RCATGY 2 cut(s) 331, 719
BstSCI CCNGG 3 cut(s) 181, 319, 454
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 2 cut(s) 261, 445
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BstZI CGGCCG 1 cut(s) 511
BsuRI GGCC 3 cut(s) 217, 513, 574
BtsCI GGATG 4 cut(s) 391, 443, 571, 800
BtsI GCAGTG 2 cut(s) 179, 555
BtsIMutI CAGTG 3 cut(s) 24, 179, 555
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 2 cut(s) 179, 461
CaiI CAGNNNCTG 1 cut(s) 712
CfoI GCGC 1 cut(s) 679
Cfr10I RCCGGY 2 cut(s) 509, 513
Cfr13I GGNCC 1 cut(s) 215
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 9 cut(s) 328, 333, 351, 363, 382, 429, 570, 620, 716
CviJI RGCY 6 cut(s) 217, 274, 459, 513, 574, 647
CviKI_1 RGCY 6 cut(s) 217, 274, 459, 513, 574, 647
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 1 cut(s) 749
DpnI GATC 5 cut(s) 45, 192, 532, 696, 711
DpnII GATC 5 cut(s) 43, 190, 530, 694, 709
DraI TTTAAA 1 cut(s) 628
EaeI YGGCCR 1 cut(s) 511
EagI CGGCCG 1 cut(s) 511
Ecl136II GAGCTC 1 cut(s) 647
EclXI CGGCCG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 649
Eco31I GGTCTC 1 cut(s) 750
Eco47III AGCGCT 1 cut(s) 678
Eco52I CGGCCG 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 3 cut(s) 152, 240, 363
Eco88I CYCGRG 1 cut(s) 872
EcoICRI GAGCTC 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 653
EcoRII CCWGG 3 cut(s) 181, 319, 454
EcoT22I ATGCAT 1 cut(s) 571
EcoT38I GRGCYC 1 cut(s) 649
FaeI CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 2 cut(s) 45, 302
FatI CATG 9 cut(s) 327, 332, 350, 362, 381, 428, 569, 619, 715
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 2 cut(s) 275, 434
FokI GGATG 4 cut(s) 398, 430, 578, 787
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 2 cut(s) 275, 434
FspBI CTAG 1 cut(s) 108
GlaI GCGC 1 cut(s) 678
GluI GCNGC 2 cut(s) 275, 434
GsaI CCCAGC 2 cut(s) 139, 694
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 3 cut(s) 217, 513, 574
HapII CCGG 2 cut(s) 510, 514
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 679
Hin1II CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 1 cut(s) 410
HindII GTYRAC 1 cut(s) 410
HinfI GANTC 3 cut(s) 305, 500, 727
HpaII CCGG 2 cut(s) 510, 514
HphI GGTGA 4 cut(s) 300, 644, 784, 859
Hpy166II GTNNAC 2 cut(s) 132, 410
Hpy188I TCNGA 5 cut(s) 48, 232, 304, 343, 674
Hpy188III TCNNGA 5 cut(s) 38, 207, 534, 650, 872
Hpy8I GTNNAC 2 cut(s) 132, 410
Hpy99I CGWCG 2 cut(s) 411, 414
HpyAV CCTTC 5 cut(s) 37, 75, 259, 275, 321
HpyCH4III ACNGT 5 cut(s) 19, 59, 156, 545, 847
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 6 cut(s) 93, 243, 359, 548, 569, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 430, 696
HpyF3I CTNAG 1 cut(s) 749
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
HspAI GCGC 1 cut(s) 677
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 5 cut(s) 43, 190, 530, 694, 709
LmnI GCTCC 3 cut(s) 271, 644, 874
Lsp1109I GCAGC 2 cut(s) 261, 445
LweI GCATC 2 cut(s) 452, 556
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 306, 632
MalI GATC 5 cut(s) 45, 192, 532, 696, 711
MboI GATC 5 cut(s) 43, 190, 530, 694, 709
MboII GAAGA 4 cut(s) 26, 158, 233, 241
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 3 cut(s) 406, 543, 649
MluCI AATT 3 cut(s) 194, 653, 880
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 571
MseI TTAA 2 cut(s) 147, 627
MspI CCGG 2 cut(s) 510, 514
MspR9I CCNGG 3 cut(s) 183, 321, 456
MvaI CCWGG 3 cut(s) 183, 321, 456
MwoI GCNNNNNNNGC 3 cut(s) 178, 430, 696
NdeII GATC 5 cut(s) 43, 190, 530, 694, 709
NlaIII CATG 9 cut(s) 331, 336, 354, 366, 385, 432, 573, 623, 719
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 2 cut(s) 306, 632
NsiI ATGCAT 1 cut(s) 571
NspI RCATGY 2 cut(s) 331, 719
PaqCI CACCTGC 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 406
PfeI GAWTC 2 cut(s) 500, 727
PkrI GCNGC 2 cut(s) 276, 435
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 649
Psp1406I AACGTT 1 cut(s) 290
Psp6I CCWGG 3 cut(s) 181, 319, 454
PspFI CCCAGC 2 cut(s) 135, 690
PspGI CCWGG 3 cut(s) 181, 319, 454
PspPI GGNCC 1 cut(s) 215
PstNI CAGNNNCTG 1 cut(s) 712
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SacI GAGCTC 1 cut(s) 649
SalI GTCGAC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 147, 627
SatI GCNGC 2 cut(s) 275, 434
Sau3AI GATC 5 cut(s) 43, 190, 530, 694, 709
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 183, 321, 456
SduI GDGCHC 3 cut(s) 406, 543, 649
SfaNI GCATC 2 cut(s) 452, 556
SgrDI CGTCGACG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 3 cut(s) 194, 653, 880
SspMI CTAG 1 cut(s) 108
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 3 cut(s) 181, 319, 454
TaaI ACNGT 5 cut(s) 19, 59, 156, 545, 847
TaiI ACGT 1 cut(s) 293
TaqI TCGA 1 cut(s) 409
TasI AATT 3 cut(s) 194, 653, 880
TfiI GAWTC 2 cut(s) 500, 727
Tru1I TTAA 2 cut(s) 147, 627
Tru9I TTAA 2 cut(s) 147, 627
TscAI CASTG 3 cut(s) 24, 179, 555
TseFI GTSAC 2 cut(s) 306, 632
TseI GCWGC 2 cut(s) 274, 433
Tsp45I GTSAC 2 cut(s) 306, 632
TspDTI ATGAA 2 cut(s) 249, 608
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 3 cut(s) 24, 179, 555
XapI RAATTY 1 cut(s) 653
XceI RCATGY 2 cut(s) 331, 719
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.