FvH4_1g00920

hemolysis in other organism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010359
Physical Location & Seq
Reverse (-)
451431 .. 453893
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00920.t1

Sequence Viewer

Length: 930 bp
ATGAGTGTTTTGACTGTGATGACCTTCAATCTACATGAAGATCCGAATGAAGATAGTTCTAATTCTTGGGAAAAGAGAAGAGATTTGTGCATAAGTGTTGTTACTAGTTATTCTCCAATGATTCTCTGTACTCAACAAGGAGCTAAATCACAACTGGATTATCTTCAACAGTGCTTGCCAGGTTATGATCAATTTGGGATATCAAGAAAAGGACCTGAAGACACTTCAGATGAACATTGCACCATCTTCTATGAGAAGGAGAAGGTGGAGCTTCTTGAAGGTGGAACATTTTGGCTGTCAGAGTCACCTTCTGTCCCTGGAAGCATGTCGTGGGGTGCTGAAGTTCCATGTATTGCTACATGGGTGACATTTCAACTGAAAGGAGTTGAGCCCCCTGGCTTTTCATTCCAGATAGTAAATACTAACATGGATGAATTCAGTCCTCGTGCACGGAGGCGGAGTGCTTTGCTTACATGGCAGCACATTGCATCCTTACCACCTAACTTGCCGGTTGTTTATTGTGGAGGGTTCAACACACAAAAGGAATCAACCACTGGTCGATTTCTTCTTGGGAGATCAAGAGAGCATGGTGTGGTGGGTGATATGAGGGATGCATGGCCTAATGCCCGTGTGAGGAAAAATGCTTCTCTTATTCGCACTTTTCATGGATTTAAAGGTAACAAACAAGGAGCTCTTGAATTCTTCAAGTTGGTTTTCAGAGCCTTGTGCCTTTGCTGGGATCGCCAAACACAGGATCTACACATAGATTGGATTCTTTTCAGAGGGAGATCTCTTGTTCCTGTTTTGTCTGAAGTGGTGAGTGATAACATTGATGGACAATATCCATCCTCCCACTATCCAGTGTTTGCCGAGTTTCTGCTTCCTCGTGCTGTGAGAATGGTTGACCCACCTGCTCAAGTGGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

310

Amino Acids

35.02

Weight (kDa)

6.0

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 7 - 276 1.7e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 919
Acc36I ACCTGC 1 cut(s) 919
AciI CCGC 1 cut(s) 457
AclWI GGATC 3 cut(s) 35, 747, 762
AcsI RAATTY 2 cut(s) 434, 698
AcuI CTGAAG 4 cut(s) 210, 237, 360, 831
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 3 cut(s) 633, 736, 751
AgsI TTSAA 7 cut(s) 28, 167, 278, 374, 532, 698, 706
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 3 cut(s) 178, 316, 394
AluBI AGCT 3 cut(s) 143, 271, 692
AluI AGCT 3 cut(s) 143, 271, 692
Alw21I GWGCWC 2 cut(s) 451, 694
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 3 cut(s) 35, 747, 762
AoxI GGCC 1 cut(s) 617
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 478
ApoI RAATTY 2 cut(s) 434, 698
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 4 cut(s) 297, 376, 611, 829
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 451
BanII GRGCYC 2 cut(s) 393, 694
BauI CACGAG 2 cut(s) 444, 885
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 451, 694
BbvI GCAGC 1 cut(s) 490
BccI CCATC 3 cut(s) 251, 827, 853
BciT130I CCWGG 3 cut(s) 180, 318, 396
BclI TGATCA 1 cut(s) 187
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 919
BglII AGATCT 1 cut(s) 788
BisI GCNGC 1 cut(s) 479
BlsI GCNGC 1 cut(s) 480
Bme1390I CCNGG 3 cut(s) 180, 318, 396
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmrFI CCNGG 3 cut(s) 180, 318, 396
BmsI GCATC 2 cut(s) 497, 601
BpiI GAAGAC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 900
BsaJI CCNNGG 2 cut(s) 316, 394
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 633, 736, 751
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 3 cut(s) 159, 559, 860
Bse3DI GCAATG 2 cut(s) 235, 483
BseBI CCWGG 3 cut(s) 180, 318, 396
BseDI CCNNGG 2 cut(s) 316, 394
BseGI GGATG 4 cut(s) 436, 488, 616, 845
BseLI CCNNNNNNNGG 3 cut(s) 633, 736, 751
BseMI GCAATG 2 cut(s) 235, 483
BseNI ACTGG 3 cut(s) 159, 559, 860
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 1 cut(s) 490
BseYI CCCAGC 1 cut(s) 735
BshFI GGCC 1 cut(s) 619
BsiHKAI GWGCWC 2 cut(s) 451, 694
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 3 cut(s) 633, 736, 751
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 1 cut(s) 619
Bsp1286I GDGCHC 3 cut(s) 393, 451, 694
Bsp143I GATC 6 cut(s) 40, 187, 575, 739, 754, 788
BspACI CCGC 1 cut(s) 457
BspANI GGCC 1 cut(s) 619
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 3 cut(s) 35, 747, 762
BsrDI GCAATG 2 cut(s) 235, 483
BsrFI RCCGGY 1 cut(s) 508
BsrI ACTGG 3 cut(s) 159, 559, 860
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 2 cut(s) 316, 394
BssMI GATC 6 cut(s) 40, 187, 575, 739, 754, 788
BssSI CACGAG 2 cut(s) 444, 885
Bst2BI CACGAG 2 cut(s) 444, 885
Bst2UI CCWGG 3 cut(s) 180, 318, 396
Bst4CI ACNGT 2 cut(s) 16, 171
Bst6I CTCTTC 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 176
BstF5I GGATG 4 cut(s) 436, 488, 616, 845
BstKTI GATC 6 cut(s) 43, 190, 578, 742, 757, 791
BstMBI GATC 6 cut(s) 40, 187, 575, 739, 754, 788
BstMWI GCNNNNNNNGC 2 cut(s) 475, 741
BstNI CCWGG 3 cut(s) 180, 318, 396
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 3 cut(s) 178, 316, 394
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 490
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 3 cut(s) 40, 754, 788
BstYI RGATCY 3 cut(s) 40, 754, 788
BsuRI GGCC 1 cut(s) 619
BtsCI GGATG 4 cut(s) 436, 488, 616, 845
BtsIMutI CAGTG 3 cut(s) 176, 552, 867
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 176
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 129
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviAII CATG 9 cut(s) 35, 325, 348, 360, 427, 474, 587, 615, 665
CviJI RGCY 8 cut(s) 143, 271, 295, 391, 399, 619, 692, 722
CviKI_1 RGCY 8 cut(s) 143, 271, 295, 391, 399, 619, 692, 722
CviQI GTAC 1 cut(s) 129
DpnI GATC 6 cut(s) 42, 189, 577, 741, 756, 790
DpnII GATC 6 cut(s) 40, 187, 575, 739, 754, 788
DraI TTTAAA 1 cut(s) 673
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
EciI GGCGGA 1 cut(s) 472
Ecl136II GAGCTC 1 cut(s) 692
Eco24I GRGCYC 2 cut(s) 393, 694
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 692
Eco57I CTGAAG 4 cut(s) 210, 237, 360, 831
EcoICRI GAGCTC 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 212
EcoRI GAATTC 2 cut(s) 434, 698
EcoRII CCWGG 3 cut(s) 178, 316, 394
EcoRV GATATC 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 616
EcoT38I GRGCYC 2 cut(s) 393, 694
FaeI CATG 9 cut(s) 38, 328, 351, 363, 430, 477, 590, 618, 668
FalI AAGNNNNNCTT 2 cut(s) 678, 710
FaqI GGGAC 1 cut(s) 299
FatI CATG 9 cut(s) 34, 324, 347, 359, 426, 473, 586, 614, 664
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 4 cut(s) 443, 475, 623, 832
FriOI GRGCYC 2 cut(s) 393, 694
Fsp4HI GCNGC 1 cut(s) 479
FspBI CTAG 1 cut(s) 105
GluI GCNGC 1 cut(s) 479
GsaI CCCAGC 1 cut(s) 739
HaeIII GGCC 1 cut(s) 619
HapII CCGG 1 cut(s) 509
Hin1II CATG 9 cut(s) 38, 328, 351, 363, 430, 477, 590, 618, 668
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HinfI GANTC 4 cut(s) 121, 302, 545, 772
HpaII CCGG 1 cut(s) 509
HphI GGTGA 4 cut(s) 297, 376, 611, 829
Hpy166II GTNNAC 2 cut(s) 449, 904
Hpy188I TCNGA 6 cut(s) 45, 229, 301, 719, 782, 811
Hpy188III TCNNGA 5 cut(s) 204, 275, 409, 579, 695
Hpy8I GTNNAC 2 cut(s) 449, 904
HpyAV CCTTC 5 cut(s) 34, 250, 256, 272, 318
HpyCH4III ACNGT 2 cut(s) 16, 171
HpyCH4V TGCA 5 cut(s) 90, 240, 449, 488, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 741
Hsp92II CATG 9 cut(s) 38, 328, 351, 363, 430, 477, 590, 618, 668
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 6 cut(s) 40, 187, 575, 739, 754, 788
LmnI GCTCC 3 cut(s) 140, 268, 689
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 2 cut(s) 497, 601
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 4 cut(s) 100, 303, 364, 677
MalI GATC 6 cut(s) 42, 189, 577, 741, 756, 790
MboI GATC 6 cut(s) 40, 187, 575, 739, 754, 788
MboII GAAGA 8 cut(s) 50, 62, 90, 155, 230, 238, 557, 694
MflI RGATCY 3 cut(s) 40, 754, 788
MhlI GDGCHC 3 cut(s) 393, 451, 694
MluCI AATT 5 cut(s) 61, 191, 434, 698, 925
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 8 cut(s) 447, 453, 518, 600, 627, 776, 859, 894
Mph1103I ATGCAT 1 cut(s) 616
MseI TTAA 1 cut(s) 672
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 3 cut(s) 180, 318, 396
MvaI CCWGG 3 cut(s) 180, 318, 396
MwoI GCNNNNNNNGC 2 cut(s) 475, 741
NdeII GATC 6 cut(s) 40, 187, 575, 739, 754, 788
NlaIII CATG 9 cut(s) 38, 328, 351, 363, 430, 477, 590, 618, 668
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 2 cut(s) 303, 364
NsiI ATGCAT 1 cut(s) 616
NspI RCATGY 1 cut(s) 328
PaqCI CACCTGC 1 cut(s) 919
PfeI GAWTC 3 cut(s) 121, 545, 772
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 212
Psp6I CCWGG 3 cut(s) 178, 316, 394
PspFI CCCAGC 1 cut(s) 735
PspGI CCWGG 3 cut(s) 178, 316, 394
PspPI GGNCC 1 cut(s) 212
PspPPI RGGWCCY 1 cut(s) 212
PsuI RGATCY 3 cut(s) 40, 754, 788
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
SacI GAGCTC 1 cut(s) 694
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 6 cut(s) 40, 187, 575, 739, 754, 788
Sau96I GGNCC 1 cut(s) 212
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 3 cut(s) 180, 318, 396
SduI GDGCHC 3 cut(s) 393, 451, 694
SfaNI GCATC 2 cut(s) 497, 601
SinI GGWCC 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 915
SmoI CTYRAG 1 cut(s) 915
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 5 cut(s) 61, 191, 434, 698, 925
SsiI CCGC 1 cut(s) 457
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 694
StyD4I CCNGG 3 cut(s) 178, 316, 394
TaaI ACNGT 2 cut(s) 16, 171
TaqI TCGA 1 cut(s) 559
TasI AATT 5 cut(s) 61, 191, 434, 698, 925
TatI WGTACW 1 cut(s) 128
TfiI GAWTC 3 cut(s) 121, 545, 772
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 3 cut(s) 176, 559, 867
TseFI GTSAC 2 cut(s) 303, 364
TseI GCWGC 1 cut(s) 478
Tsp45I GTSAC 2 cut(s) 303, 364
TspDTI ATGAA 6 cut(s) 51, 63, 246, 393, 447, 653
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 3 cut(s) 176, 559, 867
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 2 cut(s) 434, 698
XceI RCATGY 1 cut(s) 328
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.