AT4G31230

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010645
Physical Location & Seq
Reverse (-)
15172232 .. 15176292
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31230.2

Sequence Viewer

Length: 2295 bp
ATGGCGAAGAACAGGAGGGACAGAGACGGAGGATTTGGCGTGGATGGATTGGTTGCTGTGGCGATAGACAGAGACAAAAACAGCCAAGGTGCTCTGAAATGGGCTGTTGATAATTTGTTGCAGAAAGGCCAAACCGTAGTCCTCGTACATGTCAAGCCTCGGGCTTCATCTTTGTCCACAAACCCTTCAATCAACTCAAACTCTTCTAAGACGAGCCAAATAAATGGTGATTCTTCATTGGTGTGTGGAGAACCAGAAGGCTCTTATAAACAGTTGTTTCTTCCTTTTCGTTGCCTCTGTTCACGCAAAGATATACAATGCAAAGATGTGTTATTGGAGGAGTCAGATGTGGCGAGAGCTTTAGTCGAATACGCAAATCAGGTCATGATTGAGGTATTGGTGGTTGGTTCCTCCTCGAAAGGTGGCTTTCTCAGGTTCAACAAACCTATTGATATTCCAGGAGCCATCACCAAGACTGCACCTGATTTCTGCACGGTGTATGCCATAACCAAAGGAAAGCTTTCAACAAAGAAAACAGCTTCTCGTGCTGCGCCGTCTGTTTCTCCATTACGCATCCAGCTTCAACAGAATGGCTTAAAGCCACATCCACCTTTGCCTTCTGCAACTACTAACACAAGAGCTGAGAGACAGTCATTTGAGTCTCAGCATCGCAGGTCCCTTGACGATCAGTCAGATTCCTTCAGGCCACCCTACAATAAAAGAGGCTTGACTGGGAGATCATATGGAGAACTATCAATCCCGGATTCTGAAATATCCTTCAACAGCTCTGGAAGACCAAGCATAGAGCGCAACTCTCCTTCCTTATATGACAATTCGGATCCAAACCGGACTCCTCCAAGGCTATCAAATTTCTCAGACGTTGACTATTGTAGCTTCGAGTCAATGACCTTCGGAAGGAGGTCAATGGATTTGAGCTCACCAACTGCTTTCTCAACTGGCTCATTTGAGAATGAAAGGTTTTCATCGGCTTCTCAAGGAGGGGATGATGTAGAGGCTGAGATGAGGAGGCTTAAACTGGAGCTGAAACAAACGATGGAAATGTACAGTACAGCCTGTAAGGAAGCTCTCACAGCAAAACATAAGGCAACAGAGCTTCAACGTTGGAAGTTAGCAGAAGAACGGAAATTCGAAGAAGCAAAGCTTGCAGAGGAAGCTGCATTAGCCATTGCGGAGAAGGAGAAAGCAAAGTCCAAAGCAGCAATGGAAGCAGCTGAGGCAGCTCAAAGGATTGCTGATATCGAGTCCCGAAAGAGAGTCGATGCAGAAACGAAAGCTCTGAAGGAATCAGAGGCGAGAACAAAAGCCGTGAATGCTTTAGCAAAGGATGTCAGATACAGGAAGTACTCCATTGAGGAGATTGAGGATGCAACAGAGTTCTTTGATGATAAATACAAGATCGGAGAAGGCAGTTATGGACCTGTCTACAAGTGTTATCTGGATCACACACCTGTGGCTGTCAAAGCTTTGCGCCCAGATGCAGCTCAAGGCAGGTCACAGTTTCAGAAAGAGGTTGAAGTGTTATGCAGTATAAGGCACCCTAACATGGTTCTTCTGCTTGGAGCATGCCCAGAATGTGGATGCTTAGTGTATGAGTTCATGGCTAATGGGAGCTTAGAAGACCGTCTTTTCAGACAAGGAGACAGCCCGGCTCTCTCTTGGCAAACAAGATTCAGAATTGCTGCAGAGATCGGCACTGTACTGTTGTTCCTCCACCAGACTAAACCAGAACCACTGGTTCACCGTGATCTCAAACCAGCAAACATCTTACTAGACCGCAACTTTGTCAGCAAGCTTGCTGATGTTGGCTTGGCTAGGCTGGTCCCTCCATCCGTTGCAAACACCGTGACGCAATACCACATGACATCAACTGCTGGCACGTTTTGCTACATCGACCCAGAGTATCAGCAGACGGGTATGCTGGGTGTGAAATCTGACATTTATTCGCTTGGTATTATGTTTCTGCAGTTAATAACAGGAAAACCACCTATGGGTCTAACCCATTATGTCGAAAGAGCTCTCGAGAAGGGTAATTTGAAAGATTTGCTCGATCCAGCTGTGTCTGATTGGCCTGTTGAAGACACCACAGAGTTTGCCAAGTTGGCTCTCAAGTGCGCAGAGATAAGGCGAAAAGACAGACCTGATCTTTCCAAAGTTATATTGCCAGAGCTCAACAGATTAAGAGTACTTGCTGAAGAATCAACACAGGCTGCAGTAGTCATTAACAGCTCCGGACCAACCCCCACGGAGAGTCAAACTTCCTCCCCCAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Usp

Protein Analysis

764

Amino Acids

84.57

Weight (kDa)

8.21

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 370 - 625 7.3e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 373 - 626 1.7e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24370 AT4G31230 AT4G31230
fragaria_vesca FvH4_2g36560 FvH4_2g36560
malus_domestica MD15G1105300.v1.1
prunus_persica Prupe.1G457100_v2.0.a1
pyrus_communis pycom08g10450
rosa_chinensis RchiOBHm_Chr6g0305261
rosa_laevigata RLG00000010904
rosa_multiflora Rmu_ssc0000276.1_g000006
rosa_roxburghii Rroxscaffold_7G00162990 Rroxscaffold_7G00163000
rosa_rugosa Rorug06G0339100 Rorug06G0339200
rosa_samantha Rh6AG450700 Rh6BG450700 Rh6CG464800 Rh6DG451400
rosa_wichuraiana Rw6G039280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
Acc16I TGCGCA 1 cut(s) 2134
Acc36I ACCTGC 2 cut(s) 663, 1500
AccB1I GGYRCC 1 cut(s) 1554
AccB7I CCANNNNNTGG 1 cut(s) 1595
AccI GTMKAC 1 cut(s) 1443
AccIII TCCGGA 1 cut(s) 2249
AciI CCGC 2 cut(s) 1190, 1795
AclI AACGTT 1 cut(s) 1120
AclWI GGATC 4 cut(s) 833, 846, 1467, 2063
AcsI RAATTY 2 cut(s) 868, 1145
AcuI CTGAAG 3 cut(s) 685, 1319, 2232
AfaI GTAC 6 cut(s) 147, 1064, 1069, 1364, 1719, 2205
AfiI CCNNNNNNNGG 4 cut(s) 915, 1564, 1595, 2009
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 9 cut(s) 189, 439, 525, 584, 781, 1118, 1535, 2056, 2096
AhdI GACNNNNNGTC 1 cut(s) 688
AjnI CCWGG 1 cut(s) 457
AleI CACNNNNGTG 1 cut(s) 1469
AloI GAACNNNNNNTCC 2 cut(s) 741, 773
Alw21I GWGCWC 4 cut(s) 94, 938, 2038, 2190
Alw26I GTCTC 5 cut(s) 18, 66, 640, 666, 1653
AlwI GGATC 4 cut(s) 833, 846, 1467, 2063
Ama87I CYCGRG 2 cut(s) 159, 2039
Aor13HI TCCGGA 1 cut(s) 2249
AoxI GGCC 3 cut(s) 127, 704, 2087
ApeKI GCWGC 8 cut(s) 548, 1175, 1217, 1229, 1238, 1499, 1700, 2228
ApoI RAATTY 2 cut(s) 868, 1145
ArsI GACNNNNNNTTYG 4 cut(s) 17, 49, 1784, 1816
Asp700I GAANNNNTTC 1 cut(s) 520
AspLEI GCGC 4 cut(s) 553, 810, 1491, 2135
AspS9I GGNCC 4 cut(s) 675, 1436, 1840, 2252
AsuC2I CCSGG 2 cut(s) 761, 1667
AsuHPI GGTGA 4 cut(s) 239, 460, 930, 1751
AsuII TTCGAA 1 cut(s) 1149
AvaI CYCGRG 2 cut(s) 159, 2039
AvaII GGWCC 4 cut(s) 675, 1436, 1840, 2252
BamHI GGATCC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 1554
BanII GRGCYC 3 cut(s) 938, 2038, 2190
BauI CACGAG 1 cut(s) 543
BbsI GAAGAC 3 cut(s) 799, 1644, 2103
Bbv12I GWGCWC 4 cut(s) 94, 938, 2038, 2190
BbvCI CCTCAGC 1 cut(s) 1233
BbvI GCAGC 8 cut(s) 535, 1162, 1229, 1241, 1250, 1511, 1687, 2215
BccI CCATC 4 cut(s) 38, 473, 1048, 1855
BceAI ACGGC 2 cut(s) 538, 1310
BciT130I CCWGG 1 cut(s) 459
BcnI CCSGG 2 cut(s) 761, 1667
BcoDI GTCTC 5 cut(s) 18, 66, 640, 666, 1653
BfaI CTAG 2 cut(s) 1790, 1833
BfmI CTRYAG 3 cut(s) 1701, 1982, 2229
BfuAI ACCTGC 2 cut(s) 663, 1500
BglI GCCNNNNNGGC 1 cut(s) 2120
BisI GCNGC 8 cut(s) 549, 1176, 1218, 1230, 1239, 1500, 1701, 2229
BlsI GCNGC 8 cut(s) 550, 1177, 1219, 1231, 1240, 1501, 1702, 2230
BmcAI AGTACT 2 cut(s) 1364, 2205
Bme1390I CCNGG 3 cut(s) 459, 761, 1667
Bme18I GGWCC 4 cut(s) 675, 1436, 1840, 2252
BmeRI GACNNNNNGTC 1 cut(s) 688
BmeT110I CYCGRG 2 cut(s) 159, 2039
BmgT120I GGNCC 4 cut(s) 675, 1436, 1840, 2252
BmiI GGNNCC 6 cut(s) 409, 463, 677, 840, 1556, 1842
BmrFI CCNGG 3 cut(s) 459, 761, 1667
BmrI ACTGGG 1 cut(s) 741
BmsI GCATC 6 cut(s) 582, 676, 1270, 1375, 1486, 1589
BmuI ACTGGG 1 cut(s) 741
BpiI GAAGAC 3 cut(s) 799, 1644, 2103
BplI GAGNNNNNCTC 2 cut(s) 797, 829
BpmI CTGGAG 1 cut(s) 1058
Bpu10I CCTNAGC 1 cut(s) 1233
Bpu14I TTCGAA 1 cut(s) 1149
BpuEI CTTGAG 3 cut(s) 978, 1488, 2111
BpuMI CCSGG 2 cut(s) 761, 1667
BsaJI CCNNGG 4 cut(s) 85, 158, 857, 2262
BsaWI WCCGGW 2 cut(s) 846, 2249
Bsc4I CCNNNNNNNGG 4 cut(s) 915, 1564, 1595, 2009
Bse1I ACTGG 4 cut(s) 736, 961, 1041, 1758
Bse3DI GCAATG 2 cut(s) 1185, 1227
BseAI TCCGGA 1 cut(s) 2249
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 4 cut(s) 85, 158, 857, 2262
BseGI GGATG 8 cut(s) 49, 573, 604, 1009, 1351, 1390, 1604, 1847
BseLI CCNNNNNNNGG 4 cut(s) 915, 1564, 1595, 2009
BseMI GCAATG 2 cut(s) 1185, 1227
BseMII CTCAG 6 cut(s) 445, 633, 677, 888, 1008, 1224
BseNI ACTGG 4 cut(s) 736, 961, 1041, 1758
BseRI GAGGAG 5 cut(s) 353, 403, 843, 1039, 1388
BseXI GCAGC 8 cut(s) 535, 1162, 1229, 1241, 1250, 1511, 1687, 2215
BseYI CCCAGC 1 cut(s) 1939
BsgI GTGCAG 2 cut(s) 462, 475
BshFI GGCC 3 cut(s) 129, 706, 2089
BshNI GGYRCC 1 cut(s) 1554
BsiHKAI GWGCWC 4 cut(s) 94, 938, 2038, 2190
BsiHKCI CYCGRG 2 cut(s) 159, 2039
BsiSI CCGG 4 cut(s) 761, 847, 1667, 2250
BslFI GGGAC 4 cut(s) 32, 661, 1249, 1826
BslI CCNNNNNNNGG 4 cut(s) 915, 1564, 1595, 2009
BsmAI GTCTC 5 cut(s) 18, 66, 640, 666, 1653
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 4 cut(s) 32, 661, 1249, 1826
BsmI GAATGC 1 cut(s) 1336
BsnI GGCC 3 cut(s) 129, 706, 2089
BsoBI CYCGRG 2 cut(s) 159, 2039
Bsp119I TTCGAA 1 cut(s) 1149
Bsp1286I GDGCHC 4 cut(s) 94, 938, 2038, 2190
Bsp13I TCCGGA 1 cut(s) 2249
Bsp1407I TGTACA 1 cut(s) 1062
Bsp143I GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
BspACI CCGC 2 cut(s) 1190, 1795
BspANI GGCC 3 cut(s) 129, 706, 2089
BspCNI CTCAG 6 cut(s) 444, 634, 676, 887, 1009, 1225
BspEI TCCGGA 1 cut(s) 2249
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 6 cut(s) 409, 463, 677, 840, 1556, 1842
BspMAI CTGCAG 3 cut(s) 1705, 1986, 2233
BspMI ACCTGC 2 cut(s) 663, 1500
BspPI GGATC 4 cut(s) 833, 846, 1467, 2063
BspT104I TTCGAA 1 cut(s) 1149
BspT107I GGYRCC 1 cut(s) 1554
BsrDI GCAATG 2 cut(s) 1185, 1227
BsrGI TGTACA 1 cut(s) 1062
BsrI ACTGG 4 cut(s) 736, 961, 1041, 1758
BssECI CCNNGG 4 cut(s) 85, 158, 857, 2262
BssMI GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
BssSI CACGAG 1 cut(s) 543
BssT1I CCWWGG 2 cut(s) 85, 857
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 459
Bst6I CTCTTC 1 cut(s) 208
BstAPI GCANNNNNTGC 2 cut(s) 1163, 1902
BstAUI TGTACA 1 cut(s) 1062
BstBI TTCGAA 1 cut(s) 1149
BstC8I GCNNGC 5 cut(s) 1164, 1585, 1811, 1815, 1894
BstDEI CTNAG 9 cut(s) 207, 431, 642, 663, 874, 1017, 1233, 1603, 1633
BstDSI CCRYGG 1 cut(s) 2262
BstENI CCTNNNNNAGG 1 cut(s) 913
BstF5I GGATG 8 cut(s) 49, 573, 604, 1009, 1351, 1390, 1604, 1847
BstHHI GCGC 4 cut(s) 553, 810, 1491, 2135
BstKTI GATC 9 cut(s) 688, 740, 841, 1419, 1462, 1710, 1768, 2071, 2164
BstMAI GTCTC 5 cut(s) 18, 66, 640, 666, 1653
BstMBI GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 2 cut(s) 152, 1587
BstSCI CCNGG 3 cut(s) 457, 759, 1665
BstSFI CTRYAG 3 cut(s) 1701, 1982, 2229
BstV1I GCAGC 8 cut(s) 535, 1162, 1229, 1241, 1250, 1511, 1687, 2215
BstV2I GAAGAC 3 cut(s) 799, 1644, 2103
BstX2I RGATCY 1 cut(s) 838
BstXI CCANNNNNNTGG 1 cut(s) 224
BstYI RGATCY 1 cut(s) 838
BsuRI GGCC 3 cut(s) 129, 706, 2089
BtgI CCRYGG 1 cut(s) 2262
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 8 cut(s) 49, 573, 604, 1009, 1351, 1390, 1604, 1847
BtsIMutI CAGTG 2 cut(s) 1713, 1751
BveI ACCTGC 2 cut(s) 663, 1500
Cac8I GCNNGC 5 cut(s) 1164, 1585, 1811, 1815, 1894
CciI TCATGA 1 cut(s) 384
CfoI GCGC 4 cut(s) 553, 810, 1491, 2135
Cfr13I GGNCC 4 cut(s) 675, 1436, 1840, 2252
CseI GACGC 1 cut(s) 1876
Csp6I GTAC 6 cut(s) 146, 1063, 1068, 1363, 1718, 2204
CspCI CAANNNNNGTGG 2 cut(s) 2092, 2127
CviAII CATG 6 cut(s) 149, 385, 1564, 1584, 1618, 1879
CviQI GTAC 6 cut(s) 146, 1063, 1068, 1363, 1718, 2204
DdeI CTNAG 9 cut(s) 207, 431, 642, 663, 874, 1017, 1233, 1603, 1633
DpnI GATC 9 cut(s) 687, 739, 840, 1418, 1461, 1709, 1767, 2070, 2163
DpnII GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
DriI GACNNNNNGTC 1 cut(s) 688
Eam1104I CTCTTC 1 cut(s) 208
Eam1105I GACNNNNNGTC 1 cut(s) 688
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 3 cut(s) 936, 2036, 2188
Eco130I CCWWGG 2 cut(s) 85, 857
Eco24I GRGCYC 3 cut(s) 938, 2038, 2190
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 4 cut(s) 675, 1436, 1840, 2252
Eco53kI GAGCTC 3 cut(s) 936, 2036, 2188
Eco57I CTGAAG 3 cut(s) 685, 1319, 2232
Eco88I CYCGRG 2 cut(s) 159, 2039
EcoICRI GAGCTC 3 cut(s) 936, 2036, 2188
EcoNI CCTNNNNNAGG 1 cut(s) 913
EcoO109I RGGNCCY 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 457
EcoRV GATATC 1 cut(s) 1258
EcoT14I CCWWGG 2 cut(s) 85, 857
EcoT38I GRGCYC 3 cut(s) 938, 2038, 2190
ErhI CCWWGG 2 cut(s) 85, 857
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 6 cut(s) 152, 388, 1567, 1587, 1621, 1882
FalI AAGNNNNNCTT 6 cut(s) 504, 536, 1146, 1178, 1629, 1661
FaqI GGGAC 4 cut(s) 32, 661, 1249, 1826
FatI CATG 6 cut(s) 148, 384, 1563, 1583, 1617, 1878
FauNDI CATATG 1 cut(s) 742
FblI GTMKAC 1 cut(s) 1443
Fnu4HI GCNGC 8 cut(s) 549, 1176, 1218, 1230, 1239, 1500, 1701, 2229
FokI GGATG 8 cut(s) 56, 560, 591, 1016, 1358, 1397, 1611, 1834
FriOI GRGCYC 3 cut(s) 938, 2038, 2190
Fsp4HI GCNGC 8 cut(s) 549, 1176, 1218, 1230, 1239, 1500, 1701, 2229
FspBI CTAG 2 cut(s) 1790, 1833
FspI TGCGCA 1 cut(s) 2134
GlaI GCGC 4 cut(s) 552, 809, 1490, 2134
GluI GCNGC 8 cut(s) 549, 1176, 1218, 1230, 1239, 1500, 1701, 2229
GsaI CCCAGC 1 cut(s) 1943
GsuI CTGGAG 1 cut(s) 1058
HaeIII GGCC 3 cut(s) 129, 706, 2089
HapII CCGG 4 cut(s) 761, 847, 1667, 2250
HgaI GACGC 1 cut(s) 1876
HhaI GCGC 4 cut(s) 553, 810, 1491, 2135
Hin1II CATG 6 cut(s) 152, 388, 1567, 1587, 1621, 1882
Hin6I GCGC 4 cut(s) 551, 808, 1489, 2133
HinP1I GCGC 4 cut(s) 551, 808, 1489, 2133
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HindIII AAGCTT 4 cut(s) 518, 1160, 1482, 1811
HpaII CCGG 4 cut(s) 761, 847, 1667, 2250
HphI GGTGA 4 cut(s) 239, 460, 930, 1751
Hpy166II GTNNAC 5 cut(s) 177, 302, 883, 1444, 1759
Hpy188III TCNNGA 7 cut(s) 385, 789, 1266, 1457, 2039, 2041, 2250
Hpy8I GTNNAC 5 cut(s) 177, 302, 883, 1444, 1759
HpyCH4IV ACGT 3 cut(s) 879, 1120, 1898
HpyF3I CTNAG 9 cut(s) 207, 431, 642, 663, 874, 1017, 1233, 1603, 1633
HpySE526I ACGT 3 cut(s) 879, 1120, 1898
Hsp92II CATG 6 cut(s) 152, 388, 1567, 1587, 1621, 1882
HspAI GCGC 4 cut(s) 551, 808, 1489, 2133
Kpn2I TCCGGA 1 cut(s) 2249
Kzo9I GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
LmnI GCTCC 5 cut(s) 461, 1039, 1580, 1629, 2252
Lsp1109I GCAGC 8 cut(s) 535, 1162, 1229, 1241, 1250, 1511, 1687, 2215
LweI GCATC 6 cut(s) 582, 676, 1270, 1375, 1486, 1589
MaeI CTAG 2 cut(s) 1790, 1833
MaeII ACGT 3 cut(s) 879, 1120, 1898
MaeIII GTNAC 2 cut(s) 1512, 1864
MalI GATC 9 cut(s) 687, 739, 840, 1418, 1461, 1709, 1767, 2070, 2163
MboI GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 4 cut(s) 94, 938, 2038, 2190
MluCI AATT 6 cut(s) 112, 832, 868, 1145, 1695, 2050
MlyI GAGTC 7 cut(s) 350, 668, 844, 908, 1271, 1284, 2278
MmeI TCCRAC 1 cut(s) 1103
MroI TCCGGA 1 cut(s) 2249
MroXI GAANNNNTTC 1 cut(s) 520
MseI TTAA 5 cut(s) 596, 1032, 1988, 2198, 2241
MslI CAYNNNNRTG 2 cut(s) 241, 1469
MspA1I CMGCKG 2 cut(s) 1232, 2075
MspI CCGG 4 cut(s) 761, 847, 1667, 2250
MspR9I CCNGG 3 cut(s) 459, 761, 1667
Mva1269I GAATGC 1 cut(s) 1336
MvaI CCWGG 1 cut(s) 459
NciI CCSGG 2 cut(s) 761, 1667
NdeI CATATG 1 cut(s) 742
NdeII GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
NlaIII CATG 6 cut(s) 152, 388, 1567, 1587, 1621, 1882
NlaIV GGNNCC 6 cut(s) 409, 463, 677, 840, 1556, 1842
NmuCI GTSAC 2 cut(s) 1512, 1864
NsbI TGCGCA 1 cut(s) 2134
NspI RCATGY 2 cut(s) 152, 1587
NspV TTCGAA 1 cut(s) 1149
OliI CACNNNNGTG 1 cut(s) 1469
PaeI GCATGC 1 cut(s) 1587
PaeR7I CTCGAG 1 cut(s) 2039
PagI TCATGA 1 cut(s) 384
PciI ACATGT 1 cut(s) 148
PctI GAATGC 1 cut(s) 1336
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 6 cut(s) 230, 695, 764, 1304, 1689, 2216
PflMI CCANNNNNTGG 1 cut(s) 1595
PfoI TCCNGGA 2 cut(s) 457, 759
PkrI GCNGC 8 cut(s) 550, 1177, 1219, 1231, 1240, 1501, 1702, 2230
PleI GAGTC 7 cut(s) 349, 667, 844, 907, 1270, 1283, 2277
PpsI GAGTC 7 cut(s) 349, 667, 844, 907, 1270, 1283, 2277
PpuMI RGGWCCY 1 cut(s) 675
PscI ACATGT 1 cut(s) 148
PsiI TTATAA 1 cut(s) 267
Psp124BI GAGCTC 3 cut(s) 938, 2038, 2190
Psp1406I AACGTT 1 cut(s) 1120
Psp5II RGGWCCY 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 1939
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 6 cut(s) 409, 463, 677, 840, 1556, 1842
PspPI GGNCC 4 cut(s) 675, 1436, 1840, 2252
PspPPI RGGWCCY 1 cut(s) 675
PstI CTGCAG 3 cut(s) 1705, 1986, 2233
PsuI RGATCY 1 cut(s) 838
PvuII CAGCTG 2 cut(s) 1232, 2075
RsaI GTAC 6 cut(s) 147, 1064, 1069, 1364, 1719, 2205
RsaNI GTAC 6 cut(s) 146, 1063, 1068, 1363, 1718, 2204
RseI CAYNNNNRTG 2 cut(s) 241, 1469
SacI GAGCTC 3 cut(s) 938, 2038, 2190
SaqAI TTAA 5 cut(s) 596, 1032, 1988, 2198, 2241
SatI GCNGC 8 cut(s) 549, 1176, 1218, 1230, 1239, 1500, 1701, 2229
Sau3AI GATC 9 cut(s) 685, 737, 838, 1416, 1459, 1707, 1765, 2068, 2161
Sau96I GGNCC 4 cut(s) 675, 1436, 1840, 2252
ScaI AGTACT 2 cut(s) 1364, 2205
SchI GAGTC 7 cut(s) 350, 668, 844, 908, 1271, 1284, 2278
ScrFI CCNGG 3 cut(s) 459, 761, 1667
SduI GDGCHC 4 cut(s) 94, 938, 2038, 2190
SfaNI GCATC 6 cut(s) 582, 676, 1270, 1375, 1486, 1589
SfcI CTRYAG 3 cut(s) 1701, 1982, 2229
Sfr274I CTCGAG 1 cut(s) 2039
SfuI TTCGAA 1 cut(s) 1149
SinI GGWCC 4 cut(s) 675, 1436, 1840, 2252
SlaI CTCGAG 1 cut(s) 2039
SmiMI CAYNNNNRTG 2 cut(s) 241, 1469
SmlI CTYRAG 4 cut(s) 993, 1503, 2039, 2126
SmoI CTYRAG 4 cut(s) 993, 1503, 2039, 2126
SphI GCATGC 1 cut(s) 1587
Sse9I AATT 6 cut(s) 112, 832, 868, 1145, 1695, 2050
SsiI CCGC 2 cut(s) 1190, 1795
SspMI CTAG 2 cut(s) 1790, 1833
SstI GAGCTC 3 cut(s) 938, 2038, 2190
StyD4I CCNGG 3 cut(s) 457, 759, 1665
StyI CCWWGG 2 cut(s) 85, 857
TaiI ACGT 3 cut(s) 882, 1123, 1901
TasI AATT 6 cut(s) 112, 832, 868, 1145, 1695, 2050
TatI WGTACW 5 cut(s) 1062, 1067, 1362, 1717, 2203
TfiI GAWTC 6 cut(s) 230, 695, 764, 1304, 1689, 2216
Tru1I TTAA 5 cut(s) 596, 1032, 1988, 2198, 2241
Tru9I TTAA 5 cut(s) 596, 1032, 1988, 2198, 2241
TscAI CASTG 2 cut(s) 1720, 1758
TseFI GTSAC 2 cut(s) 1512, 1864
TseI GCWGC 8 cut(s) 548, 1175, 1217, 1229, 1238, 1499, 1700, 2228
Tsp45I GTSAC 2 cut(s) 1512, 1864
TspDTI ATGAA 5 cut(s) 156, 225, 972, 987, 1606
TspGWI ACGGA 4 cut(s) 42, 1156, 1840, 2279
TspRI CASTG 2 cut(s) 1720, 1758
Van91I CCANNNNNTGG 1 cut(s) 1595
VpaK11BI GGWCC 4 cut(s) 675, 1436, 1840, 2252
XagI CCTNNNNNAGG 1 cut(s) 913
XapI RAATTY 2 cut(s) 868, 1145
XceI RCATGY 2 cut(s) 152, 1587
XcmI CCANNNNNNNNNTGG 1 cut(s) 1219
XhoI CTCGAG 1 cut(s) 2039
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 2 cut(s) 1790, 1833
ZrmI AGTACT 2 cut(s) 1364, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.