MD15G1105300.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010645
Physical Location & Seq
Forward (+)
7368198 .. 7372187
3990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1105300.v1.1.491

Sequence Viewer

Length: 2337 bp
ATGTGGCTGCCCAATAATCAAGTGGACAGGAAGGAAGTAGGGAATGGGATTGTGGCAGTGGCTATAGACAAGGACAAGAACAGCCAATGTGCTCTCAAATGGGCAATTGACAATCTCATCAAACCAGGCCAATCTGTTCTTCTCATCCATGTCAAGATCAAGCCTCCTCTATTTTCTCAGTCACCTTCTCTTTCCAATTCCACCCCAAGAAGTAATCCAAATTGGGACCAACAGTTCGTCTGCAAAGAACCTGATTCCCAAACCCGGGAATTGATCCGACCTTTTCGTGTTTTTTGCGCACGGAAAAATATACAATGCAGAGACATCGTACTAGAAGACTCCGATGTGACAAAAGCATTAGTCGAATACCTTACTCAAAGTTCAATCGAGCATTTGGTTGTTGGTGCATCAGCAAAAACCGGCTTTCTTAGATTCAAAGCATCAGATATTCCAGGAAGCATATCAAAGGCGGCACCGGACTTTTGTAATGTATATGTTGTAGCCAAGCAAAAACTTCAATCAAGGAGGGCCGCCTCACGTCCTGCCCCGGCCACCTCACCCCTACGCAATCCAGCTTTTAACCACACCCCAAGCGCAAAGCCAGAGCCATATGTCTTCCCACCTTCTGGTAACAAAGGAAATGAAAAGCCACCAGAACCACAACGTAAATCCCACGATGAAATGGACAACTTCAGGTCTCCTTTTACTAGGAAAGGCCCAAATGGGAAGTCATATGCAGATCTCCCTATGCCGGATACTGACATATCTTTTGTTAGCTCCGGAAGGCCAAGCATTGACCGTATCTTCCCTGCATTTTATAACGAAAACCTAGATTCAGGAAGAGTCACCCCACCGCGAATGTCCAACAGCACGGAAATAGACTTGAACCACAGCTTTGAGTCTATGCAGTACTTTGGACGCAGGTCCGTGGATGTCAACTCCTCTCCACCCACATTCTCCTCGGTTTCTCAGGATAGCGATAGGTTATCCTCTGCATCACAGCCAATGGAAGAGGTGGAGGCTGAAATGAGAAGGCTAAAGCTGGAGCTCAAGCAAACTATGGAAATGTATAGCACCGCCTGTAAAGAGGCACTCTCGGCGAAGCAAAAGGCGGTAGAACTCCAGCGCTGGAAGTTAGAAGAAGAAAGGAGATTGGAAGAGGCACGGCTAGCTGAGGAAGCCGCAATGGCTATTGTAGAAAAGGAGAGAGCTAAGTCCAGAGCAGCCATTGAGGCTGCTGAAGCAGCTAAGAGGATTGCAGAACTAGAAGCACAAAAAAGAATCAATGCAGAAATGAAAGCACTTAAAGAACAGGAAGAGAAGAAAAAGGCACTTGACGCTCTGCAACATTCGGATGTCAGGTATAGGAGGTACTCAATTGAAGAGATTGAGGCTGCCACGGAGTTCTTTACAGAATCCCGCAAGATTGGAGAAGGAGGTTATGGTCCTGTATATAGATGTTATCTAGACCACACTCCTGTTGCAGTTAAAGTTTTACGTCCAGATGCAGCGCAAGGAAGATCACAATTCAACCAAGAGGTTGAAGTACTGAGCTGTATACGGCATCCTAACATGGTTCTCCTCCTGGGAGCCTGCCCGGAATACGGATGCTTGGTTTACGAGTACATGGCTAATGGGAGCTTGGAAGACCGTCTTCTTCGTAGAGGAAACAGCCCACCTCTCTCTTGGCAACTTAGGTTTAAGATTGCAGCTGAAATTGGGACAGGCTTGCTATTCCTCCACCAGGCCAAACCAGAGCCACTAGTCCACCGCGACTTAAAGCCCGCTAACATCTTGCTTGACCGCAACTATGTGAGCAAGATCAGCGATGTCGGCTTGGCCAGGCTTGTGCCCCCATCCGTTGCTGACACCGTGACACAGTATCGCATGACATCAACAGCCGGCACATTCTGCTACATAGACCCTGAGTATCAGCAAACCGGCATGCTTGGTGTCAAGTCTGATGTGTACTCTCTTGGGATTATGTTTCTACAAATGATCACAGCCAAGCCGGCAATGGGATTGACTCACCATGTTGAAAGGGCTATTGAGAAGGGGACTTTCGCGGATATGCTAGATCCATCCGTGCCAGATTGGCCGGTTGAAGACACATTGAAGTTTGCCAAACTGGCTCTCCAGTGTGCTGAGTTGCGACGAAAAGACAGGCCGGACCTTGGAAAAGTTATCCAGCCAGAACTTAACAGATTGAGAGGGATTGCTGAAGAGACAATGCACCCAACACTGATGGCTGGTGATCATAGCCCCTCATGCAAATACAGCCAAGTTTCGGTGCAGCTAGAGGGGAGTGAACCAGGTACAAGATCTACCGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Usp

Protein Analysis

779

Amino Acids

87.16

Weight (kDa)

8.07

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 470 - 729 1.6e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 473 - 729 1.1e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24370 AT4G31230 AT4G31230
fragaria_vesca FvH4_2g36560 FvH4_2g36560
malus_domestica MD15G1105300.v1.1
prunus_persica Prupe.1G457100_v2.0.a1
pyrus_communis pycom08g10450
rosa_chinensis RchiOBHm_Chr6g0305261
rosa_laevigata RLG00000010904
rosa_multiflora Rmu_ssc0000276.1_g000006
rosa_roxburghii Rroxscaffold_7G00162990 Rroxscaffold_7G00163000
rosa_rugosa Rorug06G0339100 Rorug06G0339200
rosa_samantha Rh6AG450700 Rh6BG450700 Rh6CG464800 Rh6DG451400
rosa_wichuraiana Rw6G039280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 819
Acc16I TGCGCA 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 912
AccB1I GGYRCC 1 cut(s) 472
AccB7I CCANNNNNTGG 1 cut(s) 626
AccI GTMKAC 1 cut(s) 1558
AccII CGCG 3 cut(s) 856, 1773, 2066
AccIII TCCGGA 1 cut(s) 779
AclWI GGATC 2 cut(s) 268, 2072
AcoI YGGCCR 3 cut(s) 549, 1839, 2096
AcuI CTGAAG 3 cut(s) 676, 1260, 2241
AfaI GTAC 7 cut(s) 330, 911, 1373, 1548, 1625, 1970, 2317
AfeI AGCGCT 1 cut(s) 1127
AfiI CCNNNNNNNGG 9 cut(s) 264, 265, 626, 751, 1604, 1744, 2018, 2174, 2287
AhlI ACTAGT 1 cut(s) 1762
AjiI CACGTC 1 cut(s) 539
AjnI CCWGG 6 cut(s) 124, 451, 1584, 1743, 1841, 2311
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
Alw21I GWGCWC 2 cut(s) 94, 1050
Alw26I GTCTC 3 cut(s) 315, 702, 2219
AlwI GGATC 2 cut(s) 268, 2072
Ama87I CYCGRG 1 cut(s) 264
Aor13HI TCCGGA 1 cut(s) 779
Aor51HI AGCGCT 1 cut(s) 1127
AoxI GGCC 9 cut(s) 127, 528, 549, 715, 785, 1746, 1839, 2096, 2165
ApeKI GCWGC 8 cut(s) 7, 1223, 1235, 1244, 1394, 1508, 1709, 2293
ArsI GACNNNNNNTTYG 6 cut(s) 218, 250, 713, 745, 752, 784
AspLEI GCGC 4 cut(s) 299, 596, 1128, 1513
AspS9I GGNCC 6 cut(s) 226, 528, 716, 924, 1445, 2170
AsuC2I CCSGG 4 cut(s) 265, 266, 548, 1598
AsuHPI GGTGA 5 cut(s) 174, 549, 838, 2021, 2264
AsuNHI GCTAGC 1 cut(s) 1168
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 4 cut(s) 226, 924, 1445, 2170
BaeGI GKGCMC 1 cut(s) 1854
BalI TGGCCA 1 cut(s) 1841
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 1050
BbsI GAAGAC 5 cut(s) 342, 607, 1646, 1653, 2112
Bbv12I GWGCWC 2 cut(s) 94, 1050
BbvCI CCTCAGC 1 cut(s) 1173
BbvI GCAGC 7 cut(s) 1222, 1235, 1256, 1381, 1520, 1721, 2305
BccI CCATC 3 cut(s) 1864, 2089, 2239
BceAI ACGGC 2 cut(s) 1181, 1577
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 6 cut(s) 126, 453, 1586, 1745, 1843, 2313
BciVI GTATCC 1 cut(s) 748
BclI TGATCA 2 cut(s) 1998, 2254
BcnI CCSGG 4 cut(s) 265, 266, 548, 1598
BcoDI GTCTC 3 cut(s) 315, 702, 2219
BcuI ACTAGT 1 cut(s) 1762
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 1129
BfuAI ACCTGC 1 cut(s) 912
BfuI GTATCC 1 cut(s) 748
BglI GCCNNNNNGGC 5 cut(s) 1187, 1232, 2012, 2095, 2129
BglII AGATCT 2 cut(s) 739, 2321
BmcAI AGTACT 2 cut(s) 911, 1548
Bme18I GGWCC 4 cut(s) 226, 924, 1445, 2170
BmeT110I CYCGRG 1 cut(s) 264
BmgBI CACGTC 1 cut(s) 539
BmgT120I GGNCC 6 cut(s) 226, 528, 716, 924, 1445, 2170
BmiI GGNNCC 3 cut(s) 227, 474, 1591
BmsI GCATC 6 cut(s) 416, 449, 1004, 1495, 1573, 1598
BmtI GCTAGC 1 cut(s) 1172
BoxI GACNNNNGTC 1 cut(s) 922
BpiI GAAGAC 5 cut(s) 342, 607, 1646, 1653, 2112
BplI GAGNNNNNCTC 2 cut(s) 1079, 1111
BpmI CTGGAG 3 cut(s) 1064, 1106, 2120
Bpu10I CCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 4 cut(s) 265, 266, 548, 1598
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 7 cut(s) 264, 546, 927, 960, 1398, 1585, 2173
BsaWI WCCGGW 2 cut(s) 475, 779
BsaXI ACNNNNNCTCC 2 cut(s) 2118, 2148
Bsc4I CCNNNNNNNGG 9 cut(s) 264, 265, 626, 751, 1604, 1744, 2018, 2174, 2287
Bse118I RCCGGY 5 cut(s) 419, 1901, 1940, 2011, 2098
Bse1I ACTGG 2 cut(s) 2133, 2137
Bse3DI GCAATG 2 cut(s) 1191, 2022
BseAI TCCGGA 1 cut(s) 779
BseBI CCWGG 6 cut(s) 126, 453, 1586, 1745, 1843, 2313
BseDI CCNNGG 7 cut(s) 264, 546, 927, 960, 1398, 1585, 2173
BseGI GGATG 7 cut(s) 144, 937, 1360, 1564, 1613, 1856, 2081
BseLI CCNNNNNNNGG 9 cut(s) 264, 265, 626, 751, 1604, 1744, 2018, 2174, 2287
BseMI GCAATG 2 cut(s) 1191, 2022
BseMII CTCAG 6 cut(s) 191, 983, 1164, 1541, 1917, 2136
BseNI ACTGG 2 cut(s) 2133, 2137
BseRI GAGGAG 4 cut(s) 156, 931, 949, 1571
BseSI GKGCMC 1 cut(s) 1854
BseXI GCAGC 7 cut(s) 1222, 1235, 1256, 1381, 1520, 1721, 2305
BsgI GTGCAG 1 cut(s) 2312
Bsh1236I CGCG 3 cut(s) 856, 1773, 2066
BshFI GGCC 9 cut(s) 129, 530, 551, 717, 787, 1748, 1841, 2098, 2167
BshNI GGYRCC 1 cut(s) 472
BsiHKAI GWGCWC 2 cut(s) 94, 1050
BsiHKCI CYCGRG 1 cut(s) 264
BslFI GGGAC 3 cut(s) 239, 1735, 2071
BslI CCNNNNNNNGG 9 cut(s) 264, 265, 626, 751, 1604, 1744, 2018, 2174, 2287
BsmAI GTCTC 3 cut(s) 315, 702, 2219
BsmFI GGGAC 3 cut(s) 239, 1735, 2071
BsnI GGCC 9 cut(s) 129, 530, 551, 717, 787, 1748, 1841, 2098, 2167
Bso31I GGTCTC 1 cut(s) 702
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 3 cut(s) 94, 1050, 1854
Bsp13I TCCGGA 1 cut(s) 779
Bsp143I GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
BspANI GGCC 9 cut(s) 129, 530, 551, 717, 787, 1748, 1841, 2098, 2167
BspCNI CTCAG 6 cut(s) 190, 982, 1165, 1542, 1918, 2137
BspEI TCCGGA 1 cut(s) 779
BspFNI CGCG 3 cut(s) 856, 1773, 2066
BspLI GGNNCC 3 cut(s) 227, 474, 1591
BspMI ACCTGC 1 cut(s) 912
BspOI GCTAGC 1 cut(s) 1172
BspPI GGATC 2 cut(s) 268, 2072
BspT107I GGYRCC 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 702
BsrDI GCAATG 2 cut(s) 1191, 2022
BsrFI RCCGGY 5 cut(s) 419, 1901, 1940, 2011, 2098
BsrI ACTGG 2 cut(s) 2133, 2137
BssAI RCCGGY 5 cut(s) 419, 1901, 1940, 2011, 2098
BssECI CCNNGG 7 cut(s) 264, 546, 927, 960, 1398, 1585, 2173
BssMI GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
BssNAI GTATAC 1 cut(s) 1559
BssT1I CCWWGG 1 cut(s) 2173
Bst1107I GTATAC 1 cut(s) 1559
Bst2UI CCWGG 6 cut(s) 126, 453, 1586, 1745, 1843, 2313
Bst4CI ACNGT 5 cut(s) 234, 800, 1652, 1873, 1881
Bst6I CTCTTC 6 cut(s) 835, 1005, 1152, 1311, 1377, 2217
BstAPI GCANNNNNTGC 1 cut(s) 1911
BstC8I GCNNGC 7 cut(s) 1170, 1594, 1730, 1785, 1903, 1946, 2013
BstDSI CCRYGG 2 cut(s) 927, 1398
BstENI CCTNNNNNAGG 1 cut(s) 1742
BstF5I GGATG 7 cut(s) 144, 937, 1360, 1564, 1613, 1856, 2081
BstFNI CGCG 3 cut(s) 856, 1773, 2066
BstH2I RGCGCY 1 cut(s) 1129
BstHHI GCGC 4 cut(s) 299, 596, 1128, 1513
BstKTI GATC 9 cut(s) 159, 276, 742, 1523, 1824, 2001, 2080, 2257, 2324
BstMAI GTCTC 3 cut(s) 315, 702, 2219
BstMBI GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
BstNI CCWGG 6 cut(s) 126, 453, 1586, 1745, 1843, 2313
BstNSI RCATGY 1 cut(s) 1948
BstPAI GACNNNNGTC 1 cut(s) 922
BstSFI CTRYAG 1 cut(s) 63
BstSLI GKGCMC 1 cut(s) 1854
BstUI CGCG 3 cut(s) 856, 1773, 2066
BstV1I GCAGC 7 cut(s) 1222, 1235, 1256, 1381, 1520, 1721, 2305
BstV2I GAAGAC 5 cut(s) 342, 607, 1646, 1653, 2112
BstX2I RGATCY 3 cut(s) 739, 2077, 2321
BstYI RGATCY 3 cut(s) 739, 2077, 2321
BstZ17I GTATAC 1 cut(s) 1559
BsuI GTATCC 1 cut(s) 748
BsuRI GGCC 9 cut(s) 129, 530, 551, 717, 787, 1748, 1841, 2098, 2167
BtgI CCRYGG 2 cut(s) 927, 1398
BtgZI GCGATG 1 cut(s) 1842
BtrI CACGTC 1 cut(s) 539
BtsCI GGATG 7 cut(s) 144, 937, 1360, 1564, 1613, 1856, 2081
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 3 cut(s) 63, 2144, 2240
BveI ACCTGC 1 cut(s) 912
Cac8I GCNNGC 7 cut(s) 1170, 1594, 1730, 1785, 1903, 1946, 2013
CfoI GCGC 4 cut(s) 299, 596, 1128, 1513
Cfr10I RCCGGY 5 cut(s) 419, 1901, 1940, 2011, 2098
Cfr13I GGNCC 6 cut(s) 226, 528, 716, 924, 1445, 2170
Cfr9I CCCGGG 1 cut(s) 264
CseI GACGC 2 cut(s) 927, 1346
CsiI ACCWGGT 1 cut(s) 2311
Csp6I GTAC 7 cut(s) 329, 910, 1372, 1547, 1624, 1969, 2316
CviAII CATG 7 cut(s) 149, 1573, 1627, 1888, 1945, 2033, 2268
CviQI GTAC 7 cut(s) 329, 910, 1372, 1547, 1624, 1969, 2316
DpnI GATC 9 cut(s) 158, 275, 741, 1522, 1823, 2000, 2079, 2256, 2323
DpnII GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
EaeI YGGCCR 3 cut(s) 549, 1839, 2096
Eam1104I CTCTTC 6 cut(s) 835, 1005, 1152, 1311, 1377, 2217
EarI CTCTTC 6 cut(s) 835, 1005, 1152, 1311, 1377, 2217
Ecl136II GAGCTC 1 cut(s) 1048
Eco130I CCWWGG 1 cut(s) 2173
Eco24I GRGCYC 1 cut(s) 1050
Eco31I GGTCTC 1 cut(s) 702
Eco47I GGWCC 4 cut(s) 226, 924, 1445, 2170
Eco47III AGCGCT 1 cut(s) 1127
Eco53kI GAGCTC 1 cut(s) 1048
Eco57I CTGAAG 3 cut(s) 676, 1260, 2241
Eco88I CYCGRG 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 1048
EcoNI CCTNNNNNAGG 1 cut(s) 1742
EcoRII CCWGG 6 cut(s) 124, 451, 1584, 1743, 1841, 2311
EcoT14I CCWWGG 1 cut(s) 2173
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 1 cut(s) 2173
FaeI CATG 7 cut(s) 152, 1576, 1630, 1891, 1948, 2036, 2271
FalI AAGNNNNNCTT 2 cut(s) 1638, 1670
FaqI GGGAC 3 cut(s) 239, 1735, 2071
FatI CATG 7 cut(s) 148, 1572, 1626, 1887, 1944, 2032, 2267
FauI CCCGC 2 cut(s) 1427, 1792
FauNDI CATATG 2 cut(s) 610, 733
FbaI TGATCA 2 cut(s) 1998, 2254
FblI GTMKAC 1 cut(s) 1558
FokI GGATG 7 cut(s) 131, 944, 1367, 1551, 1620, 1843, 2068
FriOI GRGCYC 1 cut(s) 1050
FspI TGCGCA 1 cut(s) 298
GlaI GCGC 4 cut(s) 298, 595, 1127, 1512
GsuI CTGGAG 3 cut(s) 1064, 1106, 2120
HaeII RGCGCY 1 cut(s) 1129
HaeIII GGCC 9 cut(s) 129, 530, 551, 717, 787, 1748, 1841, 2098, 2167
HgaI GACGC 2 cut(s) 927, 1346
HhaI GCGC 4 cut(s) 299, 596, 1128, 1513
Hin1II CATG 7 cut(s) 152, 1576, 1630, 1891, 1948, 2036, 2271
Hin6I GCGC 4 cut(s) 297, 594, 1126, 1511
HinP1I GCGC 4 cut(s) 297, 594, 1126, 1511
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HinfI GANTC 9 cut(s) 254, 338, 432, 833, 843, 899, 1281, 1415, 2026
HphI GGTGA 5 cut(s) 174, 549, 838, 2021, 2264
Hpy166II GTNNAC 7 cut(s) 25, 937, 1559, 1618, 1768, 1969, 2309
Hpy188I TCNGA 5 cut(s) 278, 343, 445, 1354, 1963
Hpy188III TCNNGA 7 cut(s) 154, 780, 837, 971, 1218, 1466, 1502
Hpy8I GTNNAC 7 cut(s) 25, 937, 1559, 1618, 1768, 1969, 2309
Hpy99I CGWCG 1 cut(s) 2157
HpyAV CCTTC 7 cut(s) 25, 195, 633, 777, 1026, 1427, 2047
HpyCH4III ACNGT 5 cut(s) 234, 800, 1652, 1873, 1881
HpyCH4IV ACGT 3 cut(s) 538, 664, 1498
HpySE526I ACGT 3 cut(s) 538, 664, 1498
Hsp92II CATG 7 cut(s) 152, 1576, 1630, 1891, 1948, 2036, 2271
HspAI GCGC 4 cut(s) 297, 594, 1126, 1511
Kpn2I TCCGGA 1 cut(s) 779
KroI GCCGGC 2 cut(s) 1901, 2011
KroNI GCCGGC 2 cut(s) 1903, 2013
Ksp22I TGATCA 2 cut(s) 1998, 2254
Kzo9I GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
LmnI GCTCC 4 cut(s) 782, 1045, 1589, 1638
Lsp1109I GCAGC 7 cut(s) 1222, 1235, 1256, 1381, 1520, 1721, 2305
LweI GCATC 6 cut(s) 416, 449, 1004, 1495, 1573, 1598
MabI ACCWGGT 1 cut(s) 2311
MaeII ACGT 3 cut(s) 538, 664, 1498
MaeIII GTNAC 5 cut(s) 180, 346, 629, 844, 1873
MalI GATC 9 cut(s) 158, 275, 741, 1522, 1823, 2000, 2079, 2256, 2323
MboI GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
MfeI CAATTG 2 cut(s) 105, 1377
MflI RGATCY 3 cut(s) 739, 2077, 2321
MhlI GDGCHC 3 cut(s) 94, 1050, 1854
MlsI TGGCCA 1 cut(s) 1841
MluCI AATT 7 cut(s) 105, 196, 220, 269, 1377, 1526, 1716
MluNI TGGCCA 1 cut(s) 1841
MlyI GAGTC 4 cut(s) 332, 852, 908, 2020
MmeI TCCRAC 2 cut(s) 301, 888
Mox20I TGGCCA 1 cut(s) 1841
MroI TCCGGA 1 cut(s) 779
MroNI GCCGGC 2 cut(s) 1901, 2011
MscI TGGCCA 1 cut(s) 1841
MseI TTAA 6 cut(s) 579, 1305, 1488, 1701, 1778, 2199
MslI CAYNNNNRTG 1 cut(s) 1353
Msp20I TGGCCA 1 cut(s) 1841
MspA1I CMGCKG 1 cut(s) 1712
MunI CAATTG 2 cut(s) 105, 1377
MvaI CCWGG 6 cut(s) 126, 453, 1586, 1745, 1843, 2313
MvnI CGCG 3 cut(s) 856, 1773, 2066
NaeI GCCGGC 2 cut(s) 1903, 2013
NciI CCSGG 4 cut(s) 265, 266, 548, 1598
NdeI CATATG 2 cut(s) 610, 733
NdeII GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
NgoMIV GCCGGC 2 cut(s) 1901, 2011
NheI GCTAGC 1 cut(s) 1168
NlaIII CATG 7 cut(s) 152, 1576, 1630, 1891, 1948, 2036, 2271
NlaIV GGNNCC 3 cut(s) 227, 474, 1591
NmeAIII GCCGAG 1 cut(s) 1076
NmuCI GTSAC 4 cut(s) 180, 346, 844, 1873
NsbI TGCGCA 1 cut(s) 298
NspI RCATGY 1 cut(s) 1948
PaeI GCATGC 1 cut(s) 1948
PdiI GCCGGC 2 cut(s) 1903, 2013
PfeI GAWTC 5 cut(s) 254, 432, 833, 1281, 1415
PflMI CCANNNNNTGG 1 cut(s) 626
PfoI TCCNGGA 1 cut(s) 451
PleI GAGTC 4 cut(s) 332, 851, 907, 2020
PpsI GAGTC 4 cut(s) 332, 851, 907, 2020
PshAI GACNNNNGTC 1 cut(s) 922
PsiI TTATAA 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 1050
Psp6I CCWGG 6 cut(s) 124, 451, 1584, 1743, 1841, 2311
PspGI CCWGG 6 cut(s) 124, 451, 1584, 1743, 1841, 2311
PspN4I GGNNCC 3 cut(s) 227, 474, 1591
PspPI GGNCC 6 cut(s) 226, 528, 716, 924, 1445, 2170
PsuI RGATCY 3 cut(s) 739, 2077, 2321
PvuII CAGCTG 1 cut(s) 1712
RsaI GTAC 7 cut(s) 330, 911, 1373, 1548, 1625, 1970, 2317
RsaNI GTAC 7 cut(s) 329, 910, 1372, 1547, 1624, 1969, 2316
RseI CAYNNNNRTG 1 cut(s) 1353
SacI GAGCTC 1 cut(s) 1050
SaqAI TTAA 6 cut(s) 579, 1305, 1488, 1701, 1778, 2199
Sau3AI GATC 9 cut(s) 156, 273, 739, 1520, 1821, 1998, 2077, 2254, 2321
Sau96I GGNCC 6 cut(s) 226, 528, 716, 924, 1445, 2170
ScaI AGTACT 2 cut(s) 911, 1548
SchI GAGTC 4 cut(s) 332, 852, 908, 2020
SduI GDGCHC 3 cut(s) 94, 1050, 1854
SexAI ACCWGGT 1 cut(s) 2311
SfaNI GCATC 6 cut(s) 416, 449, 1004, 1495, 1573, 1598
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 4 cut(s) 226, 924, 1445, 2170
SmaI CCCGGG 1 cut(s) 266
SmiMI CAYNNNNRTG 1 cut(s) 1353
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
SpeI ACTAGT 1 cut(s) 1762
SphI GCATGC 1 cut(s) 1948
Sse9I AATT 7 cut(s) 105, 196, 220, 269, 1377, 1526, 1716
SstI GAGCTC 1 cut(s) 1050
StyI CCWWGG 1 cut(s) 2173
TaaI ACNGT 5 cut(s) 234, 800, 1652, 1873, 1881
TaiI ACGT 3 cut(s) 541, 667, 1501
TaqI TCGA 2 cut(s) 363, 387
TasI AATT 7 cut(s) 105, 196, 220, 269, 1377, 1526, 1716
TatI WGTACW 4 cut(s) 909, 1546, 1623, 1968
TauI GCSGC 3 cut(s) 473, 533, 1184
TfiI GAWTC 5 cut(s) 254, 432, 833, 1281, 1415
Tru1I TTAA 6 cut(s) 579, 1305, 1488, 1701, 1778, 2199
Tru9I TTAA 6 cut(s) 579, 1305, 1488, 1701, 1778, 2199
TscAI CASTG 3 cut(s) 63, 2144, 2247
TseFI GTSAC 4 cut(s) 180, 346, 844, 1873
TseI GCWGC 8 cut(s) 7, 1223, 1235, 1244, 1394, 1508, 1709, 2293
Tsp45I GTSAC 4 cut(s) 180, 346, 844, 1873
TspDTI ATGAA 3 cut(s) 657, 693, 1310
TspGWI ACGGA 7 cut(s) 316, 887, 916, 1415, 1620, 1849, 2074
TspMI CCCGGG 1 cut(s) 264
TspRI CASTG 3 cut(s) 63, 2144, 2247
Van91I CCANNNNNTGG 1 cut(s) 626
VpaK11BI GGWCC 4 cut(s) 226, 924, 1445, 2170
XagI CCTNNNNNAGG 1 cut(s) 1742
XbaI TCTAGA 1 cut(s) 1465
XceI RCATGY 1 cut(s) 1948
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 1683, 2014
XmaI CCCGGG 1 cut(s) 264
XmiI GTMKAC 1 cut(s) 1558
ZrmI AGTACT 2 cut(s) 911, 1548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.