Rorug06G0339200

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010645
Physical Location & Seq
Reverse (-)
49073430 .. 49078223
4794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0339200.1

Sequence Viewer

Length: 1341 bp
ATGGCGGAGGGGAGGAAGGAGAGGAGGCCGAGCAGCGAAGAGAGCGTGAAGCTGTTCGTCGGCCAAGTGCCCAAGCACATGAACGAAGCTCAACTCCTCGCAATGTTCCAAGAGTTCTCGCTCGTCGACGAGGTCAACATCATCAAGGACAAGACCACGCGCGCGTCTAGAGGGTGCTGCTTTGTGATATGTCCGTCGAGGGAGGAGGCAGATAAGGCGGTCAATGCATGTCACAATAAGAAGACCTTGCCCGGGGCATCAAGTCCTTTGCAAGTGAAGTATGCTGATGGCGAGTTGGAAAGACTAGAACATAAGCTCTTTGTCGGTATGCTTCCAAAGAATGTTTCCGAAGTTGAAGTATCCGATCTTTTCTCTAAATACGGAACAGTAAAGGACCTACAGATATTAAGGGGTTCTCAGCAAACGAGCAAAGGCTGTGCATTTTTAAAGTATGAGACAAAAGAACAAGCACTTGCTGCCCTGGAAGCCATTAATGGAAAACACAAAATGGAGGGTTCAAGTGTTCCTTTGGTTGTCAAATGGGCAGACACAGAAAAGGAAAGGCTAGCTCGGAGAGCCCAAAAAGCTCATTCTCAGGCTGCTAATGTGCCAAATTCTGATTCACAACCTCCATCTTTATTTGGAGCCTTGCCTATGGGTTATGTTCCCTCATATAATGGGTATAGTTATCAGGCTCCTGGAACATATGGCCTTATGCCATACCGCCTGCCAGCACTGCAAAATCAACCAGGTTTCCATAATATGATACCCCCTGTAAATCAAGGAAATGCTTTACATCGAATTACACCCGACCTTCCTCCCAATATTGCCCCTAGAAATTTTGCTATGCGTCCAAGTTACCTGGGTTCTGGTTATGCTGCGGTTCCAGGTCTTCAGCATCCGATGGTATATCCGGGAGGAATGGTGAGTCACCGGCCATTGAGCGCCTCACCTGGTTCATTGTCGCCTCTAGTTGTGAACAGTAACCCAGCCACATCCTTAGGCACCAGTAGAAGTTCCGGGGGTCAGGTTGAAGGTCCTCCTGGTGCTAATTTATTTATCTACCACATACCTCAAGAATTTGGAGATCAAGAGCTTGCTAATGCTTTTCAAGCATTTGGTAGGGTTTTGAGTGCCAAGGTTTTTGTTGATAAAGCAACCGGTGTCAGCAAATGTTTTGGATTTGTAAGTTATGATTCACCAGAGGCTGCTCAATCTGCTATCACTGTTATGAATGGATACCAATTAAGTGAAGTGTTTCCTGGGCTCATTGGCGTCATGATGCAGGCTGCATGTAGACCACTGCATCTGTGGTCTCAGTTGTTGCTTCTTTTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Usp

Protein Analysis

446

Amino Acids

48.42

Weight (kDa)

8.8

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 18 - 81 6.1e-13 RNA recognition motif
RRM_1 PF00076 106 - 169 1.3e-16 RNA recognition motif
RRM_1 PF00076 352 - 416 5.9e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24370 AT4G31230 AT4G31230
fragaria_vesca FvH4_2g36560 FvH4_2g36560
malus_domestica MD15G1105300.v1.1
prunus_persica Prupe.1G457100_v2.0.a1
pyrus_communis pycom08g10450
rosa_chinensis RchiOBHm_Chr6g0305261
rosa_laevigata RLG00000010904
rosa_multiflora Rmu_ssc0000276.1_g000006
rosa_roxburghii Rroxscaffold_7G00162990 Rroxscaffold_7G00163000
rosa_rugosa Rorug06G0339100 Rorug06G0339200
rosa_samantha Rh6AG450700 Rh6BG450700 Rh6CG464800 Rh6DG451400
rosa_wichuraiana Rw6G039280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1004
AccI GTMKAC 2 cut(s) 126, 1297
AccII CGCG 3 cut(s) 160, 162, 164
AciI CCGC 4 cut(s) 5, 218, 724, 881
AcoI YGGCCR 2 cut(s) 61, 935
AcsI RAATTY 3 cut(s) 613, 838, 1079
AcuI CTGAAG 1 cut(s) 878
AcyI GRCGYC 1 cut(s) 1275
AfiI CCNNNNNNNGG 1 cut(s) 252
AgeI ACCGGT 1 cut(s) 1160
AgsI TTSAA 4 cut(s) 356, 519, 1034, 1112
AjnI CCWGG 8 cut(s) 480, 697, 748, 861, 886, 952, 1042, 1261
AjuI GAANNNNNNNTTGG 4 cut(s) 328, 360, 604, 636
AluBI AGCT 6 cut(s) 52, 89, 316, 569, 587, 1096
AluI AGCT 6 cut(s) 52, 89, 316, 569, 587, 1096
Alw26I GTCTC 2 cut(s) 449, 1320
AlwNI CAGNNNCTG 1 cut(s) 1208
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 4 cut(s) 26, 61, 709, 935
ApeKI GCWGC 7 cut(s) 33, 177, 476, 599, 878, 1208, 1289
ApoI RAATTY 3 cut(s) 613, 838, 1079
AseI ATTAAT 1 cut(s) 492
AsiGI ACCGGT 1 cut(s) 1160
Asp700I GAANNNNTTC 2 cut(s) 53, 1257
AspLEI GCGC 3 cut(s) 162, 164, 947
AspS9I GGNCC 2 cut(s) 394, 1037
AsuC2I CCSGG 4 cut(s) 252, 253, 915, 1021
AsuHPI GGTGA 4 cut(s) 923, 937, 942, 1191
AsuNHI GCTAGC 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 2 cut(s) 394, 1037
AxyI CCTNAGG 1 cut(s) 1000
BaeGI GKGCMC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 1004
BanII GRGCYC 2 cut(s) 580, 1269
BbsI GAAGAC 2 cut(s) 248, 884
BbvI GCAGC 7 cut(s) 45, 164, 463, 586, 865, 1195, 1276
BccI CCATC 3 cut(s) 281, 640, 898
BciT130I CCWGG 8 cut(s) 482, 699, 750, 863, 888, 954, 1044, 1263
BciVI GTATCC 2 cut(s) 370, 1232
BcnI CCSGG 4 cut(s) 252, 253, 915, 1021
BcoDI GTCTC 2 cut(s) 449, 1320
BfaI CTAG 5 cut(s) 168, 305, 566, 834, 971
BfmI CTRYAG 1 cut(s) 398
BfoI RGCGCY 1 cut(s) 948
BfuI GTATCC 2 cut(s) 370, 1232
BisI GCNGC 7 cut(s) 34, 178, 477, 600, 879, 1209, 1290
BlsI GCNGC 7 cut(s) 35, 179, 478, 601, 880, 1210, 1291
Bme18I GGWCC 2 cut(s) 394, 1037
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 394, 1037
BmiI GGNNCC 4 cut(s) 646, 696, 885, 1006
BmsI GCATC 4 cut(s) 266, 907, 1272, 1315
BmtI GCTAGC 1 cut(s) 569
BpiI GAAGAC 2 cut(s) 248, 884
BpuEI CTTGAG 1 cut(s) 1059
BpuMI CCSGG 4 cut(s) 252, 253, 915, 1021
BsaHI GRCGYC 1 cut(s) 1275
BsaI GGTCTC 1 cut(s) 1320
BsaJI CCNNGG 7 cut(s) 251, 252, 480, 862, 1020, 1137, 1262
BsaWI WCCGGW 1 cut(s) 1160
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse118I RCCGGY 2 cut(s) 933, 1160
Bse1I ACTGG 1 cut(s) 1008
Bse21I CCTNAGG 1 cut(s) 1000
Bse3DI GCAATG 1 cut(s) 108
BseBI CCWGG 8 cut(s) 482, 699, 750, 863, 888, 954, 1044, 1263
BseDI CCNNGG 7 cut(s) 251, 252, 480, 862, 1020, 1137, 1262
BseGI GGATG 2 cut(s) 898, 995
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 3 cut(s) 431, 608, 1331
BseNI ACTGG 1 cut(s) 1008
BsePI GCGCGC 1 cut(s) 160
BseRI GAGGAG 3 cut(s) 37, 86, 218
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 7 cut(s) 45, 164, 463, 586, 865, 1195, 1276
BseYI CCCAGC 1 cut(s) 988
Bsh1236I CGCG 3 cut(s) 160, 162, 164
BshFI GGCC 4 cut(s) 28, 63, 711, 937
BshNI GGYRCC 1 cut(s) 1004
BshTI ACCGGT 1 cut(s) 1160
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 5 cut(s) 252, 914, 934, 1020, 1161
BslI CCNNNNNNNGG 1 cut(s) 252
BsmAI GTCTC 2 cut(s) 449, 1320
BsnI GGCC 4 cut(s) 28, 63, 711, 937
Bso31I GGTCTC 1 cut(s) 1320
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 72, 580, 1269
Bsp143I GATC 2 cut(s) 364, 1087
BspACI CCGC 4 cut(s) 5, 218, 724, 881
BspANI GGCC 4 cut(s) 28, 63, 711, 937
BspCNI CTCAG 3 cut(s) 430, 607, 1330
BspFNI CGCG 3 cut(s) 160, 162, 164
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 4 cut(s) 646, 696, 885, 1006
BspOI GCTAGC 1 cut(s) 569
BspT107I GGYRCC 1 cut(s) 1004
BspTNI GGTCTC 1 cut(s) 1320
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 933, 1160
BsrI ACTGG 1 cut(s) 1008
BssAI RCCGGY 2 cut(s) 933, 1160
BssECI CCNNGG 7 cut(s) 251, 252, 480, 862, 1020, 1137, 1262
BssHII GCGCGC 1 cut(s) 160
BssMI GATC 2 cut(s) 364, 1087
BssNI GRCGYC 1 cut(s) 1275
BssT1I CCWWGG 1 cut(s) 1137
Bst2UI CCWGG 8 cut(s) 482, 699, 750, 863, 888, 954, 1044, 1263
Bst4CI ACNGT 3 cut(s) 388, 983, 1228
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 1275
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 6 cut(s) 162, 567, 728, 732, 1098, 1287
BstDEI CTNAG 4 cut(s) 417, 594, 1000, 1317
BstF5I GGATG 2 cut(s) 898, 995
BstFNI CGCG 3 cut(s) 160, 162, 164
BstH2I RGCGCY 1 cut(s) 948
BstHHI GCGC 3 cut(s) 162, 164, 947
BstKTI GATC 2 cut(s) 367, 1090
BstMAI GTCTC 2 cut(s) 449, 1320
BstMBI GATC 2 cut(s) 364, 1087
BstNI CCWGG 8 cut(s) 482, 699, 750, 863, 888, 954, 1044, 1263
BstNSI RCATGY 2 cut(s) 231, 1296
BstSFI CTRYAG 1 cut(s) 398
BstSLI GKGCMC 1 cut(s) 72
BstUI CGCG 3 cut(s) 160, 162, 164
BstV1I GCAGC 7 cut(s) 45, 164, 463, 586, 865, 1195, 1276
BstV2I GAAGAC 2 cut(s) 248, 884
Bsu36I CCTNAGG 1 cut(s) 1000
BsuI GTATCC 2 cut(s) 370, 1232
BsuRI GGCC 4 cut(s) 28, 63, 711, 937
BtsCI GGATG 2 cut(s) 898, 995
BtsI GCAGTG 2 cut(s) 734, 1301
BtsIMutI CAGTG 3 cut(s) 734, 1224, 1301
Cac8I GCNNGC 6 cut(s) 162, 567, 728, 732, 1098, 1287
CaiI CAGNNNCTG 1 cut(s) 1208
CciI TCATGA 1 cut(s) 1278
CfoI GCGC 3 cut(s) 162, 164, 947
Cfr10I RCCGGY 2 cut(s) 933, 1160
Cfr13I GGNCC 2 cut(s) 394, 1037
Cfr9I CCCGGG 1 cut(s) 251
CseI GACGC 3 cut(s) 153, 839, 1264
CsiI ACCWGGT 2 cut(s) 748, 952
CspAI ACCGGT 1 cut(s) 1160
CviAII CATG 4 cut(s) 79, 228, 1279, 1293
DdeI CTNAG 4 cut(s) 417, 594, 1000, 1317
DpnI GATC 2 cut(s) 366, 1089
DpnII GATC 2 cut(s) 364, 1087
DraI TTTAAA 1 cut(s) 447
EaeI YGGCCR 2 cut(s) 61, 935
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 1137
Eco24I GRGCYC 2 cut(s) 580, 1269
Eco31I GGTCTC 1 cut(s) 1320
Eco47I GGWCC 2 cut(s) 394, 1037
Eco57I CTGAAG 1 cut(s) 878
Eco81I CCTNAGG 1 cut(s) 1000
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 2 cut(s) 394, 1037
EcoRII CCWGG 8 cut(s) 480, 697, 748, 861, 886, 952, 1042, 1261
EcoT14I CCWWGG 1 cut(s) 1137
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 2 cut(s) 580, 1269
ErhI CCWWGG 1 cut(s) 1137
FaeI CATG 4 cut(s) 82, 231, 1282, 1296
FalI AAGNNNNNCTT 4 cut(s) 230, 262, 511, 543
FatI CATG 4 cut(s) 78, 227, 1278, 1292
FauNDI CATATG 1 cut(s) 706
FblI GTMKAC 2 cut(s) 126, 1297
Fnu4HI GCNGC 7 cut(s) 34, 178, 477, 600, 879, 1209, 1290
FokI GGATG 2 cut(s) 885, 982
FriOI GRGCYC 2 cut(s) 580, 1269
Fsp4HI GCNGC 7 cut(s) 34, 178, 477, 600, 879, 1209, 1290
FspBI CTAG 5 cut(s) 168, 305, 566, 834, 971
GlaI GCGC 3 cut(s) 161, 163, 946
GluI GCNGC 7 cut(s) 34, 178, 477, 600, 879, 1209, 1290
GsaI CCCAGC 1 cut(s) 992
HaeII RGCGCY 1 cut(s) 948
HaeIII GGCC 4 cut(s) 28, 63, 711, 937
HapII CCGG 5 cut(s) 252, 914, 934, 1020, 1161
HgaI GACGC 3 cut(s) 153, 839, 1264
HhaI GCGC 3 cut(s) 162, 164, 947
Hin1I GRCGYC 1 cut(s) 1275
Hin1II CATG 4 cut(s) 82, 231, 1282, 1296
Hin6I GCGC 3 cut(s) 160, 162, 945
HinP1I GCGC 3 cut(s) 160, 162, 945
HincII GTYRAC 2 cut(s) 127, 136
HindII GTYRAC 2 cut(s) 127, 136
HinfI GANTC 3 cut(s) 620, 928, 1196
HpaII CCGG 5 cut(s) 252, 914, 934, 1020, 1161
HphI GGTGA 4 cut(s) 923, 937, 942, 1191
Hpy166II GTNNAC 4 cut(s) 127, 136, 979, 1298
Hpy188I TCNGA 5 cut(s) 349, 364, 573, 619, 903
Hpy188III TCNNGA 4 cut(s) 168, 1076, 1091, 1279
Hpy8I GTNNAC 4 cut(s) 127, 136, 979, 1298
Hpy99I CGWCG 4 cut(s) 62, 128, 131, 199
HpyAV CCTTC 3 cut(s) 10, 824, 1028
HpyCH4III ACNGT 3 cut(s) 388, 983, 1228
HpyCH4V TGCA 7 cut(s) 227, 271, 440, 739, 1285, 1292, 1306
HpyF3I CTNAG 4 cut(s) 417, 594, 1000, 1317
Hsp92I GRCGYC 1 cut(s) 1275
Hsp92II CATG 4 cut(s) 82, 231, 1282, 1296
HspAI GCGC 3 cut(s) 160, 162, 945
Kzo9I GATC 2 cut(s) 364, 1087
LmnI GCTCC 2 cut(s) 644, 700
Lsp1109I GCAGC 7 cut(s) 45, 164, 463, 586, 865, 1195, 1276
LweI GCATC 4 cut(s) 266, 907, 1272, 1315
MabI ACCWGGT 2 cut(s) 748, 952
MaeI CTAG 5 cut(s) 168, 305, 566, 834, 971
MaeIII GTNAC 4 cut(s) 230, 857, 929, 983
MalI GATC 2 cut(s) 366, 1089
MauBI CGCGCGCG 1 cut(s) 160
MboI GATC 2 cut(s) 364, 1087
MboII GAAGA 3 cut(s) 50, 253, 884
MhlI GDGCHC 3 cut(s) 72, 580, 1269
MluCI AATT 6 cut(s) 613, 801, 838, 1051, 1079, 1244
MlyI GAGTC 1 cut(s) 937
MmeI TCCRAC 1 cut(s) 276
Mph1103I ATGCAT 1 cut(s) 229
MroXI GAANNNNTTC 2 cut(s) 53, 1257
MseI TTAA 4 cut(s) 407, 446, 492, 1247
MslI CAYNNNNRTG 1 cut(s) 1229
MspI CCGG 5 cut(s) 252, 914, 934, 1020, 1161
MvaI CCWGG 8 cut(s) 482, 699, 750, 863, 888, 954, 1044, 1263
MvnI CGCG 3 cut(s) 160, 162, 164
NciI CCSGG 4 cut(s) 252, 253, 915, 1021
NdeI CATATG 1 cut(s) 706
NdeII GATC 2 cut(s) 364, 1087
NheI GCTAGC 1 cut(s) 565
NlaIII CATG 4 cut(s) 82, 231, 1282, 1296
NlaIV GGNNCC 4 cut(s) 646, 696, 885, 1006
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 230, 929
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 2 cut(s) 231, 1296
PagI TCATGA 1 cut(s) 1278
PauI GCGCGC 1 cut(s) 160
PdmI GAANNNNTTC 2 cut(s) 53, 1257
PfeI GAWTC 2 cut(s) 620, 1196
PflFI GACNNNGTC 1 cut(s) 131
PfoI TCCNGGA 2 cut(s) 697, 913
PinAI ACCGGT 1 cut(s) 1160
PkrI GCNGC 7 cut(s) 35, 179, 478, 601, 880, 1210, 1291
PleI GAGTC 1 cut(s) 936
PpsI GAGTC 1 cut(s) 936
PpuMI RGGWCCY 2 cut(s) 394, 1037
PshBI ATTAAT 1 cut(s) 492
Psp5II RGGWCCY 2 cut(s) 394, 1037
Psp6I CCWGG 8 cut(s) 480, 697, 748, 861, 886, 952, 1042, 1261
PspFI CCCAGC 1 cut(s) 988
PspGI CCWGG 8 cut(s) 480, 697, 748, 861, 886, 952, 1042, 1261
PspN4I GGNNCC 4 cut(s) 646, 696, 885, 1006
PspPI GGNCC 2 cut(s) 394, 1037
PspPPI RGGWCCY 2 cut(s) 394, 1037
PstNI CAGNNNCTG 1 cut(s) 1208
PsyI GACNNNGTC 1 cut(s) 131
PteI GCGCGC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 1229
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 4 cut(s) 407, 446, 492, 1247
SatI GCNGC 7 cut(s) 34, 178, 477, 600, 879, 1209, 1290
Sau3AI GATC 2 cut(s) 364, 1087
Sau96I GGNCC 2 cut(s) 394, 1037
SchI GAGTC 1 cut(s) 937
SduI GDGCHC 3 cut(s) 72, 580, 1269
SexAI ACCWGGT 2 cut(s) 748, 952
SfaNI GCATC 4 cut(s) 266, 907, 1272, 1315
SfcI CTRYAG 1 cut(s) 398
SgrDI CGTCGACG 1 cut(s) 125
SinI GGWCC 2 cut(s) 394, 1037
SmaI CCCGGG 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 1229
SmlI CTYRAG 1 cut(s) 1074
SmoI CTYRAG 1 cut(s) 1074
Sse9I AATT 6 cut(s) 613, 801, 838, 1051, 1079, 1244
SsiI CCGC 4 cut(s) 5, 218, 724, 881
SspI AATATT 1 cut(s) 826
SspMI CTAG 5 cut(s) 168, 305, 566, 834, 971
StyI CCWWGG 1 cut(s) 1137
TaaI ACNGT 3 cut(s) 388, 983, 1228
TaqI TCGA 3 cut(s) 126, 197, 799
TasI AATT 6 cut(s) 613, 801, 838, 1051, 1079, 1244
TfiI GAWTC 2 cut(s) 620, 1196
Tru1I TTAA 4 cut(s) 407, 446, 492, 1247
Tru9I TTAA 4 cut(s) 407, 446, 492, 1247
TscAI CASTG 3 cut(s) 741, 1231, 1308
TseFI GTSAC 2 cut(s) 230, 929
TseI GCWGC 7 cut(s) 33, 177, 476, 599, 878, 1208, 1289
Tsp45I GTSAC 2 cut(s) 230, 929
TspDTI ATGAA 3 cut(s) 95, 948, 1247
TspGWI ACGGA 2 cut(s) 183, 396
TspMI CCCGGG 1 cut(s) 251
TspRI CASTG 3 cut(s) 741, 1231, 1308
Tth111I GACNNNGTC 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 394, 1037
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 3 cut(s) 613, 838, 1079
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 2 cut(s) 231, 1296
XcmI CCANNNNNNNNNTGG 1 cut(s) 1308
XmaI CCCGGG 1 cut(s) 251
XmiI GTMKAC 2 cut(s) 126, 1297
XmnI GAANNNNTTC 2 cut(s) 53, 1257
XspI CTAG 5 cut(s) 168, 305, 566, 834, 971
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.