pycom08g10450

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010645
Physical Location & Seq
Forward (+)
8731450 .. 8734776
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10450.4

Sequence Viewer

Length: 2262 bp
ATGTGGCTAGCCAATAATCAAGTGGACAAGAAGGAAGCAGGGAATGGGATTGTGGCAGTGGCAATAGACAGGGACAAGAACAGCCAATGTGCTCTCAAATGGGCAATTGACAGTCTCATCAAACCAGGCCAATCTGTTCTTCTCATCCATGTCAAGGTCAAGCATTCCATAATTTCTCAGTCGCATTCTCTTTCCAATTCCACCCCAAGAAGTAATCCAAATTGGGACCAACAGTTGGTATGCAAGGACCCTGATCCCCAAACCCGGGAATTGTTCCTTCCCTTTCGTGTTTTTTGCACACGTAAAAATGGCGTTACGTTGCTTCGTATGCCTCGAAATTCCACAGAACATTGTCTCATTTTTTTTAAAATTTTATGTAGATTCAAAGCATCAGATATTCCAGGAAACATATCAAAGGCGGCACCAGACTTTTGTAATGTATATGTTGTATCCAAGCAAAAACTCCAATCAAGGAGGGCCGCCTCACGTCCTGCCCCGGCTGTCTCACCCCTACGCAACCCAGCTCGTAACCACACCCCAAGCCCAAAGTCAGAGCCATACGTCTTCCCTCCTTCTGGTAACAAAGGAAATGAAAAGCCACCAGAACCGCCACGTCAATCCCACGATGAAATGGACTACTTCAGGTCTCCGTTTACTAGGAAAGGCCCGAACGGGAAGTCATATGCAGATCTCCCTATGCCGGATACTGATATATCTTTTGTTAGCTCTGGAAGGCCGAGCATTGACCGTATCTTCCCTGCATTTTATAACGAAAACCTAGATTCAGGAAGAGTCACCCCACCACGAATGTCCAACAGCACGGAAATAGACTTGAACCACAGCTTTGAGTCTATGCAGTACTTTGGACGGAGGTCCGTGGATGTCAGCTCCTCTCCACCCACATTCTCTTCGGTTTCTCAGGATAGTGATAGGTTATCCTCTGCATCAATGGAAGACATGGAGGCTGAAATGAGAAGGCTAAAGCTGGAGCTCAAGCAAACTATGGAAATGTATAGCACTGCCTGTAAAGAGGCACTCTCGGCGAAGCAAAAGGCTGTAGAACTCCAGCGCTGGAAATTAGAAGAAGAAAGGAGATTGGAAGAGGCGCGGCTAGCTGAGGAAACCGCAATGGCTATTGTAGAAAAGGAGAGAGCAAAGTCCAGAGCAGCCATCGAGGCTGCTGAAGCAGCTAAGAGGATTGCAGAACTGGAAGCACAAAAAAGAATCAATGCAGAAATGAAAGCACTCAAAGAACACGAAGAGAAGAAGAAGGCACTTAGCGCTCTGCAACAGTCGGATGTCAGGTATAGGAAGTACTCAATTGAAGAGATTGAGGCTGCCACGGAGTTCTTTACAGAATCCCGCAAGATTGGAGAAGGAGGTTACGGTCCTGTATATAGGTGTAATCTTGACCATACTCCTGTTGCAATTAAGGTTTTACGTCCAGATGCTGCACAAGGAAGATCACAGTTCAACCAAGAGGTTGAAGTATTGAGCTCTATACGGCATCCTAACATGGTTCTTCTGCTGGGAGCCTGCCCGGAGTACGGATGCTTGGTTTATGAGTACATGGCTAATGGGAGCTTGGAAGACCGTCTTCTTCGTAGAGGAAACAGTCCAATTCTCTCTTGGCAACTTAGATTTAAGATTGCAGCTGAAATTGGGACAGGCTTGCTATTCCTCCACCAGGCCAAACCAGAGCCACTAGTCCACCGCGACTTAAAACCCGCTAACATCTTGCTTGACCGCAACTATGTGAGCAAGATCAGCGATGTTGGCTTGGCCAGGCTTGTGCCCCCATCTGTTGCTGACACCGTGACGCAGTATCGCATGACATCAACAGCTGGCACGTTCTGCTACATAGACCCTGAGTATCAGCAAACTGGCATGCTTGGTGTTAAGTCTGATGTGTACTCTCTTGGGATTATGTTTCTGCAAATGATCACAGCCAAGCCGGCAATGGGATTGACTCACCATGTTGAAAGGGCTATTGAGAAGGGGACTTTCGAAGATATGCTGGATCCATCAGTGCCAGATTGGCCGGTTCAAGACACATTGAAGTTTGCCAAGCTGGCTCTCCAGTGTGCAGAGTTGCGGCGAAAAGACAGGCCGGACCTTCGAAAGGTTATCCTGCCAGAACTTAACAGATTGAGAGGGATTGCTGAAGAGACAATGCACCCAACACTGATGGCTGGTGGTCATAGCCCCTCGTCCGAACACAGCCAAGTTTCTGTGCAGCTAGTAAGTGTATATGGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Usp

Protein Analysis

754

Amino Acids

84.67

Weight (kDa)

8.82

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010645)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G24370 AT4G31230 AT4G31230
fragaria_vesca FvH4_2g36560 FvH4_2g36560
malus_domestica MD15G1105300.v1.1
prunus_persica Prupe.1G457100_v2.0.a1
pyrus_communis pycom08g10450
rosa_chinensis RchiOBHm_Chr6g0305261
rosa_laevigata RLG00000010904
rosa_multiflora Rmu_ssc0000276.1_g000006
rosa_roxburghii Rroxscaffold_7G00162990 Rroxscaffold_7G00163000
rosa_rugosa Rorug06G0339100 Rorug06G0339200
rosa_samantha Rh6AG450700 Rh6BG450700 Rh6CG464800 Rh6DG451400
rosa_wichuraiana Rw6G039280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 235
AccII CGCG 2 cut(s) 1108, 1716
AclWI GGATC 3 cut(s) 248, 2015, 2028
AcoI YGGCCR 2 cut(s) 1782, 2039
AcsI RAATTY 2 cut(s) 337, 369
AcuI CTGAAG 3 cut(s) 625, 1203, 2184
AfaI GTAC 5 cut(s) 860, 1316, 1547, 1568, 1913
AfeI AGCGCT 2 cut(s) 1070, 1282
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 8 cut(s) 385, 835, 1325, 1474, 1487, 1982, 2048, 2059
AhlI ACTAGT 1 cut(s) 1705
AjiI CACGTC 2 cut(s) 488, 614
AjnI CCWGG 4 cut(s) 124, 400, 1686, 1784
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
Alw21I GWGCWC 3 cut(s) 94, 993, 1499
Alw26I GTCTC 5 cut(s) 119, 359, 508, 651, 2162
AlwI GGATC 3 cut(s) 248, 2015, 2028
AlwNI CAGNNNCTG 1 cut(s) 1451
Ama87I CYCGRG 1 cut(s) 264
Aor51HI AGCGCT 2 cut(s) 1070, 1282
AoxI GGCC 8 cut(s) 127, 477, 664, 734, 1689, 1782, 2039, 2108
ApeKI GCWGC 7 cut(s) 1166, 1178, 1187, 1337, 1451, 1652, 2236
ApoI RAATTY 2 cut(s) 337, 369
ArsI GACNNNNNNTTYG 2 cut(s) 662, 694
AspLEI GCGC 3 cut(s) 1071, 1108, 1283
AspS9I GGNCC 7 cut(s) 226, 247, 477, 665, 873, 1388, 2113
AsuC2I CCSGG 4 cut(s) 265, 266, 497, 1541
AsuHPI GGTGA 3 cut(s) 498, 787, 1964
AsuII TTCGAA 2 cut(s) 2007, 2119
AsuNHI GCTAGC 2 cut(s) 7, 1111
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 5 cut(s) 226, 247, 873, 1388, 2113
BaeGI GKGCMC 1 cut(s) 1797
BalI TGGCCA 1 cut(s) 1784
BamHI GGATCC 1 cut(s) 2020
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 2 cut(s) 993, 1499
BbsI GAAGAC 4 cut(s) 556, 960, 1589, 1596
Bbv12I GWGCWC 3 cut(s) 94, 993, 1499
BbvCI CCTCAGC 1 cut(s) 1116
BbvI GCAGC 7 cut(s) 1165, 1178, 1199, 1324, 1438, 1664, 2248
BccI CCATC 4 cut(s) 1178, 1807, 2032, 2182
BceAI ACGGC 1 cut(s) 1520
BciT130I CCWGG 4 cut(s) 126, 402, 1688, 1786
BciVI GTATCC 2 cut(s) 460, 697
BclI TGATCA 1 cut(s) 1941
BcnI CCSGG 4 cut(s) 265, 266, 497, 1541
BcoDI GTCTC 5 cut(s) 119, 359, 508, 651, 2162
BcuI ACTAGT 1 cut(s) 1705
BfaI CTAG 6 cut(s) 8, 657, 779, 1112, 1706, 2240
BfmI CTRYAG 1 cut(s) 1056
BfoI RGCGCY 2 cut(s) 1072, 1284
BfuI GTATCC 2 cut(s) 460, 697
BglI GCCNNNNNGGC 4 cut(s) 1175, 1955, 2038, 2072
BglII AGATCT 1 cut(s) 688
BmcAI AGTACT 2 cut(s) 860, 1316
Bme1390I CCNGG 8 cut(s) 126, 265, 266, 402, 497, 1541, 1688, 1786
Bme18I GGWCC 5 cut(s) 226, 247, 873, 1388, 2113
BmeT110I CYCGRG 1 cut(s) 264
BmgBI CACGTC 2 cut(s) 488, 614
BmgT120I GGNCC 7 cut(s) 226, 247, 477, 665, 873, 1388, 2113
BmiI GGNNCC 5 cut(s) 227, 249, 423, 1534, 2022
BmrFI CCNGG 8 cut(s) 126, 265, 266, 402, 497, 1541, 1688, 1786
BmsI GCATC 5 cut(s) 398, 953, 1438, 1516, 1541
BmtI GCTAGC 2 cut(s) 11, 1115
BoxI GACNNNNGTC 1 cut(s) 871
BpiI GAAGAC 4 cut(s) 556, 960, 1589, 1596
BplI GAGNNNNNCTC 2 cut(s) 1022, 1054
BpmI CTGGAG 3 cut(s) 1007, 1049, 2063
Bpu10I CCTNAGC 1 cut(s) 1116
Bpu14I TTCGAA 2 cut(s) 2007, 2119
BpuEI CTTGAG 1 cut(s) 977
BpuMI CCSGG 4 cut(s) 265, 266, 497, 1541
BsaAI YACGTR 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 651
BsaJI CCNNGG 4 cut(s) 264, 495, 876, 1341
Bse118I RCCGGY 2 cut(s) 1954, 2041
Bse1I ACTGG 3 cut(s) 1212, 1888, 2080
Bse3DI GCAATG 2 cut(s) 1134, 1965
BseBI CCWGG 4 cut(s) 126, 402, 1688, 1786
BseDI CCNNGG 4 cut(s) 264, 495, 876, 1341
BseGI GGATG 5 cut(s) 144, 886, 1303, 1507, 1556
BseMI GCAATG 2 cut(s) 1134, 1965
BseMII CTCAG 4 cut(s) 191, 932, 1107, 1860
BseNI ACTGG 3 cut(s) 1212, 1888, 2080
BseRI GAGGAG 1 cut(s) 880
BseSI GKGCMC 1 cut(s) 1797
BseXI GCAGC 7 cut(s) 1165, 1178, 1199, 1324, 1438, 1664, 2248
BseYI CCCAGC 2 cut(s) 520, 1528
BsgI GTGCAG 3 cut(s) 1437, 2106, 2255
Bsh1236I CGCG 2 cut(s) 1108, 1716
BshFI GGCC 8 cut(s) 129, 479, 666, 736, 1691, 1784, 2041, 2110
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 3 cut(s) 94, 993, 1499
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 7 cut(s) 265, 497, 701, 1541, 1955, 2042, 2111
BslFI GGGAC 4 cut(s) 86, 239, 1678, 2014
BsmAI GTCTC 5 cut(s) 119, 359, 508, 651, 2162
BsmFI GGGAC 4 cut(s) 86, 239, 1678, 2014
BsmI GAATGC 2 cut(s) 163, 184
BsnI GGCC 8 cut(s) 129, 479, 666, 736, 1691, 1784, 2041, 2110
Bso31I GGTCTC 1 cut(s) 651
BsoBI CYCGRG 1 cut(s) 264
Bsp119I TTCGAA 2 cut(s) 2007, 2119
Bsp1286I GDGCHC 4 cut(s) 94, 993, 1499, 1797
Bsp143I GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
BspANI GGCC 8 cut(s) 129, 479, 666, 736, 1691, 1784, 2041, 2110
BspCNI CTCAG 4 cut(s) 190, 931, 1108, 1861
BspFNI CGCG 2 cut(s) 1108, 1716
BspLI GGNNCC 5 cut(s) 227, 249, 423, 1534, 2022
BspOI GCTAGC 2 cut(s) 11, 1115
BspPI GGATC 3 cut(s) 248, 2015, 2028
BspT104I TTCGAA 2 cut(s) 2007, 2119
BspT107I GGYRCC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 651
BsrDI GCAATG 2 cut(s) 1134, 1965
BsrFI RCCGGY 2 cut(s) 1954, 2041
BsrI ACTGG 3 cut(s) 1212, 1888, 2080
BssAI RCCGGY 2 cut(s) 1954, 2041
BssECI CCNNGG 4 cut(s) 264, 495, 876, 1341
BssMI GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
Bst2UI CCWGG 4 cut(s) 126, 402, 1688, 1786
Bst4CI ACNGT 9 cut(s) 113, 234, 749, 1293, 1388, 1470, 1595, 1616, 1816
Bst6I CTCTTC 6 cut(s) 784, 913, 1095, 1254, 1320, 2160
BstAPI GCANNNNNTGC 1 cut(s) 1854
BstBAI YACGTR 1 cut(s) 302
BstBI TTCGAA 2 cut(s) 2007, 2119
BstC8I GCNNGC 8 cut(s) 9, 1113, 1537, 1673, 1846, 1889, 1956, 2073
BstDEI CTNAG 7 cut(s) 177, 918, 1116, 1191, 1277, 1637, 1869
BstDSI CCRYGG 2 cut(s) 876, 1341
BstENI CCTNNNNNAGG 2 cut(s) 1685, 2120
BstF5I GGATG 5 cut(s) 144, 886, 1303, 1507, 1556
BstFNI CGCG 2 cut(s) 1108, 1716
BstH2I RGCGCY 2 cut(s) 1072, 1284
BstHHI GCGC 3 cut(s) 1071, 1108, 1283
BstKTI GATC 6 cut(s) 256, 691, 1466, 1767, 1944, 2023
BstMAI GTCTC 5 cut(s) 119, 359, 508, 651, 2162
BstMBI GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
BstNI CCWGG 4 cut(s) 126, 402, 1688, 1786
BstNSI RCATGY 1 cut(s) 1891
BstPAI GACNNNNGTC 1 cut(s) 871
BstSCI CCNGG 8 cut(s) 124, 263, 264, 400, 495, 1539, 1686, 1784
BstSFI CTRYAG 1 cut(s) 1056
BstSLI GKGCMC 1 cut(s) 1797
BstUI CGCG 2 cut(s) 1108, 1716
BstV1I GCAGC 7 cut(s) 1165, 1178, 1199, 1324, 1438, 1664, 2248
BstV2I GAAGAC 4 cut(s) 556, 960, 1589, 1596
BstX2I RGATCY 2 cut(s) 688, 2020
BstYI RGATCY 2 cut(s) 688, 2020
BsuI GTATCC 2 cut(s) 460, 697
BsuRI GGCC 8 cut(s) 129, 479, 666, 736, 1691, 1784, 2041, 2110
BtgI CCRYGG 2 cut(s) 876, 1341
BtgZI GCGATG 1 cut(s) 1785
BtrI CACGTC 2 cut(s) 488, 614
BtsCI GGATG 5 cut(s) 144, 886, 1303, 1507, 1556
BtsI GCAGTG 2 cut(s) 63, 1017
BtsIMutI CAGTG 5 cut(s) 63, 1017, 2034, 2087, 2183
Cac8I GCNNGC 8 cut(s) 9, 1113, 1537, 1673, 1846, 1889, 1956, 2073
CaiI CAGNNNCTG 1 cut(s) 1451
CfoI GCGC 3 cut(s) 1071, 1108, 1283
Cfr10I RCCGGY 2 cut(s) 1954, 2041
Cfr13I GGNCC 7 cut(s) 226, 247, 477, 665, 873, 1388, 2113
Cfr9I CCCGGG 1 cut(s) 264
CseI GACGC 1 cut(s) 1828
Csp6I GTAC 5 cut(s) 859, 1315, 1546, 1567, 1912
CviAII CATG 7 cut(s) 149, 958, 1516, 1570, 1831, 1888, 1976
CviQI GTAC 5 cut(s) 859, 1315, 1546, 1567, 1912
DdeI CTNAG 7 cut(s) 177, 918, 1116, 1191, 1277, 1637, 1869
DpnI GATC 6 cut(s) 255, 690, 1465, 1766, 1943, 2022
DpnII GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
DraI TTTAAA 1 cut(s) 367
EaeI YGGCCR 2 cut(s) 1782, 2039
Eam1104I CTCTTC 6 cut(s) 784, 913, 1095, 1254, 1320, 2160
EarI CTCTTC 6 cut(s) 784, 913, 1095, 1254, 1320, 2160
Ecl136II GAGCTC 2 cut(s) 991, 1497
Eco24I GRGCYC 2 cut(s) 993, 1499
Eco31I GGTCTC 1 cut(s) 651
Eco47I GGWCC 5 cut(s) 226, 247, 873, 1388, 2113
Eco47III AGCGCT 2 cut(s) 1070, 1282
Eco53kI GAGCTC 2 cut(s) 991, 1497
Eco57I CTGAAG 3 cut(s) 625, 1203, 2184
Eco88I CYCGRG 1 cut(s) 264
EcoICRI GAGCTC 2 cut(s) 991, 1497
EcoNI CCTNNNNNAGG 2 cut(s) 1685, 2120
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 4 cut(s) 124, 400, 1686, 1784
EcoT38I GRGCYC 2 cut(s) 993, 1499
FaeI CATG 7 cut(s) 152, 961, 1519, 1573, 1834, 1891, 1979
FalI AAGNNNNNCTT 2 cut(s) 1581, 1613
FaqI GGGAC 4 cut(s) 86, 239, 1678, 2014
FatI CATG 7 cut(s) 148, 957, 1515, 1569, 1830, 1887, 1975
FauI CCCGC 2 cut(s) 1370, 1735
FauNDI CATATG 1 cut(s) 682
FbaI TGATCA 1 cut(s) 1941
FokI GGATG 5 cut(s) 131, 893, 1310, 1494, 1563
FriOI GRGCYC 2 cut(s) 993, 1499
FspBI CTAG 6 cut(s) 8, 657, 779, 1112, 1706, 2240
GlaI GCGC 3 cut(s) 1070, 1107, 1282
GsaI CCCAGC 2 cut(s) 524, 1532
GsuI CTGGAG 3 cut(s) 1007, 1049, 2063
HaeII RGCGCY 2 cut(s) 1072, 1284
HaeIII GGCC 8 cut(s) 129, 479, 666, 736, 1691, 1784, 2041, 2110
HapII CCGG 7 cut(s) 265, 497, 701, 1541, 1955, 2042, 2111
HgaI GACGC 1 cut(s) 1828
HhaI GCGC 3 cut(s) 1071, 1108, 1283
Hin1II CATG 7 cut(s) 152, 961, 1519, 1573, 1834, 1891, 1979
Hin6I GCGC 3 cut(s) 1069, 1106, 1281
HinP1I GCGC 3 cut(s) 1069, 1106, 1281
HinfI GANTC 7 cut(s) 381, 782, 792, 848, 1224, 1358, 1969
HpaII CCGG 7 cut(s) 265, 497, 701, 1541, 1955, 2042, 2111
HphI GGTGA 3 cut(s) 498, 787, 1964
Hpy166II GTNNAC 4 cut(s) 25, 654, 1711, 1912
Hpy188I TCNGA 5 cut(s) 394, 553, 1297, 1906, 2215
Hpy188III TCNNGA 7 cut(s) 729, 786, 920, 1161, 1409, 1445, 2048
Hpy8I GTNNAC 4 cut(s) 25, 654, 1711, 1912
HpyAV CCTTC 9 cut(s) 25, 287, 582, 726, 969, 1264, 1370, 1990, 2126
HpyCH4III ACNGT 9 cut(s) 113, 234, 749, 1293, 1388, 1470, 1595, 1616, 1816
HpyCH4IV ACGT 7 cut(s) 301, 317, 487, 561, 613, 1441, 1850
HpyF3I CTNAG 7 cut(s) 177, 918, 1116, 1191, 1277, 1637, 1869
HpySE526I ACGT 7 cut(s) 301, 317, 487, 561, 613, 1441, 1850
Hsp92II CATG 7 cut(s) 152, 961, 1519, 1573, 1834, 1891, 1979
HspAI GCGC 3 cut(s) 1069, 1106, 1281
KroI GCCGGC 1 cut(s) 1954
KroNI GCCGGC 1 cut(s) 1956
Ksp22I TGATCA 1 cut(s) 1941
Kzo9I GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
LmnI GCTCC 4 cut(s) 893, 988, 1532, 1581
Lsp1109I GCAGC 7 cut(s) 1165, 1178, 1199, 1324, 1438, 1664, 2248
LweI GCATC 5 cut(s) 398, 953, 1438, 1516, 1541
MaeI CTAG 6 cut(s) 8, 657, 779, 1112, 1706, 2240
MaeII ACGT 7 cut(s) 301, 317, 487, 561, 613, 1441, 1850
MaeIII GTNAC 6 cut(s) 313, 527, 578, 793, 1382, 1816
MalI GATC 6 cut(s) 255, 690, 1465, 1766, 1943, 2022
MboI GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
MfeI CAATTG 2 cut(s) 105, 1320
MflI RGATCY 2 cut(s) 688, 2020
MhlI GDGCHC 4 cut(s) 94, 993, 1499, 1797
MlsI TGGCCA 1 cut(s) 1784
MluNI TGGCCA 1 cut(s) 1784
MlyI GAGTC 3 cut(s) 801, 857, 1963
MmeI TCCRAC 2 cut(s) 837, 1275
Mox20I TGGCCA 1 cut(s) 1784
MroNI GCCGGC 1 cut(s) 1954
MscI TGGCCA 1 cut(s) 1784
MseI TTAA 6 cut(s) 366, 1431, 1644, 1721, 1899, 2142
Msp20I TGGCCA 1 cut(s) 1784
MspA1I CMGCKG 2 cut(s) 1655, 1844
MspI CCGG 7 cut(s) 265, 497, 701, 1541, 1955, 2042, 2111
MspR9I CCNGG 8 cut(s) 126, 265, 266, 402, 497, 1541, 1688, 1786
MunI CAATTG 2 cut(s) 105, 1320
Mva1269I GAATGC 2 cut(s) 163, 184
MvaI CCWGG 4 cut(s) 126, 402, 1688, 1786
MvnI CGCG 2 cut(s) 1108, 1716
NaeI GCCGGC 1 cut(s) 1956
NciI CCSGG 4 cut(s) 265, 266, 497, 1541
NdeI CATATG 1 cut(s) 682
NdeII GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
NgoMIV GCCGGC 1 cut(s) 1954
NheI GCTAGC 2 cut(s) 7, 1111
NlaIII CATG 7 cut(s) 152, 961, 1519, 1573, 1834, 1891, 1979
NlaIV GGNNCC 5 cut(s) 227, 249, 423, 1534, 2022
NmeAIII GCCGAG 2 cut(s) 762, 1019
NmuCI GTSAC 2 cut(s) 793, 1816
NspI RCATGY 1 cut(s) 1891
NspV TTCGAA 2 cut(s) 2007, 2119
PaeI GCATGC 1 cut(s) 1891
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PctI GAATGC 2 cut(s) 163, 184
PdiI GCCGGC 1 cut(s) 1956
PfeI GAWTC 4 cut(s) 381, 782, 1224, 1358
PflMI CCANNNNNTGG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 400
PleI GAGTC 3 cut(s) 800, 856, 1963
PpsI GAGTC 3 cut(s) 800, 856, 1963
Ppu21I YACGTR 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 247
PshAI GACNNNNGTC 1 cut(s) 871
PsiI TTATAA 1 cut(s) 768
Psp124BI GAGCTC 2 cut(s) 993, 1499
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 4 cut(s) 124, 400, 1686, 1784
PspFI CCCAGC 2 cut(s) 520, 1528
PspGI CCWGG 4 cut(s) 124, 400, 1686, 1784
PspN4I GGNNCC 5 cut(s) 227, 249, 423, 1534, 2022
PspPI GGNCC 7 cut(s) 226, 247, 477, 665, 873, 1388, 2113
PspPPI RGGWCCY 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 1451
PsuI RGATCY 2 cut(s) 688, 2020
PvuII CAGCTG 2 cut(s) 1655, 1844
RsaI GTAC 5 cut(s) 860, 1316, 1547, 1568, 1913
RsaNI GTAC 5 cut(s) 859, 1315, 1546, 1567, 1912
SacI GAGCTC 2 cut(s) 993, 1499
SaqAI TTAA 6 cut(s) 366, 1431, 1644, 1721, 1899, 2142
Sau3AI GATC 6 cut(s) 253, 688, 1463, 1764, 1941, 2020
Sau96I GGNCC 7 cut(s) 226, 247, 477, 665, 873, 1388, 2113
ScaI AGTACT 2 cut(s) 860, 1316
SchI GAGTC 3 cut(s) 801, 857, 1963
ScrFI CCNGG 8 cut(s) 126, 265, 266, 402, 497, 1541, 1688, 1786
SduI GDGCHC 4 cut(s) 94, 993, 1499, 1797
SfaNI GCATC 5 cut(s) 398, 953, 1438, 1516, 1541
SfcI CTRYAG 1 cut(s) 1056
SfuI TTCGAA 2 cut(s) 2007, 2119
SinI GGWCC 5 cut(s) 226, 247, 873, 1388, 2113
SmaI CCCGGG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
SpeI ACTAGT 1 cut(s) 1705
SphI GCATGC 1 cut(s) 1891
SspMI CTAG 6 cut(s) 8, 657, 779, 1112, 1706, 2240
SstI GAGCTC 2 cut(s) 993, 1499
StyD4I CCNGG 8 cut(s) 124, 263, 264, 400, 495, 1539, 1686, 1784
TaaI ACNGT 9 cut(s) 113, 234, 749, 1293, 1388, 1470, 1595, 1616, 1816
TaiI ACGT 7 cut(s) 304, 320, 490, 564, 616, 1444, 1853
TaqI TCGA 4 cut(s) 334, 1173, 2007, 2119
TatI WGTACW 4 cut(s) 858, 1314, 1566, 1911
TauI GCSGC 4 cut(s) 422, 482, 1111, 2098
TfiI GAWTC 4 cut(s) 381, 782, 1224, 1358
Tru1I TTAA 6 cut(s) 366, 1431, 1644, 1721, 1899, 2142
Tru9I TTAA 6 cut(s) 366, 1431, 1644, 1721, 1899, 2142
TscAI CASTG 5 cut(s) 63, 1024, 2034, 2087, 2190
TseFI GTSAC 2 cut(s) 793, 1816
TseI GCWGC 7 cut(s) 1166, 1178, 1187, 1337, 1451, 1652, 2236
Tsp45I GTSAC 2 cut(s) 793, 1816
TspDTI ATGAA 3 cut(s) 606, 642, 1253
TspGWI ACGGA 6 cut(s) 639, 836, 865, 883, 1358, 1563
TspMI CCCGGG 1 cut(s) 264
TspRI CASTG 5 cut(s) 63, 1024, 2034, 2087, 2190
Van91I CCANNNNNTGG 1 cut(s) 235
VpaK11BI GGWCC 5 cut(s) 226, 247, 873, 1388, 2113
XagI CCTNNNNNAGG 2 cut(s) 1685, 2120
XapI RAATTY 2 cut(s) 337, 369
XceI RCATGY 1 cut(s) 1891
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 1626, 1957
XmaI CCCGGG 1 cut(s) 264
XspI CTAG 6 cut(s) 8, 657, 779, 1112, 1706, 2240
ZrmI AGTACT 2 cut(s) 860, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.