AT4G31740

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005697
Physical Location & Seq
Forward (+)
15363778 .. 15364349
572 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31740.1

Sequence Viewer

Length: 516 bp
ATGGACAAGTTACGATTTGCCATCATGTACCTTCTCTCGTTGGAAACTATCAACCAGTCTGAAGTTGAAGCCGTGGAAGCGGCATTGCCCTCGGCAGACTCAGCAAGCAGAAGCAACATTGTTGACTGGGCTGAGAAGCTTTATGGTCAGTCTATAAGCGCGGTCACTCCAGGAGTCAAGAATCTCTTATCAAGTGATCAGCAACTGCCGGTGGCACGGACAGTTGAAGCCTTAACTGACGGAAAACCAAACCCAGAAACCGATTCATACCTCATACTGGATGCGAGAGCTTCAAAGTCAGGATCTATTGATAATAGCTATGTGAAGGGGCCTTTCGAAGAAGCCATAGTCTTCATGATTGGTGGAGGTAACTACATTGAATATAGCAGTTTGCAGGAGCTCTCGCAGCGACAGGAGATGGTTAATAACATTATATATGGAGCCACAGAGATCCTTACTGGAACTGAGCTCGTTGAGCAGCTCGGGGAGTTGGGTCAGAAGATGGGACTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

171

Amino Acids

18.62

Weight (kDa)

4.4

Isoelectric Point (pI)

50.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 2 - 160 5e-17 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 161
AciI CCGC 2 cut(s) 80, 161
AclWI GGATC 2 cut(s) 310, 445
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 4 cut(s) 68, 227, 294, 380
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 6 cut(s) 139, 290, 318, 400, 469, 481
AluI AGCT 6 cut(s) 139, 290, 318, 400, 469, 481
Alw21I GWGCWC 2 cut(s) 402, 471
AlwI GGATC 2 cut(s) 310, 445
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 482
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 406, 478
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 336
AvaI CYCGRG 1 cut(s) 482
BanII GRGCYC 2 cut(s) 402, 471
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 2 cut(s) 402, 471
BbvI GCAGC 2 cut(s) 418, 490
BccI CCATC 3 cut(s) 29, 412, 496
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 1 cut(s) 171
BclI TGATCA 1 cut(s) 196
BisI GCNGC 3 cut(s) 81, 407, 479
BlsI GCNGC 3 cut(s) 82, 408, 480
Bme1390I CCNGG 1 cut(s) 171
BmeT110I CYCGRG 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 330, 442
BmrFI CCNGG 1 cut(s) 171
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 271
BmuI ACTGGG 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 343
BpmI CTGGAG 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 72, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 4 cut(s) 55, 131, 282, 463
Bse3DI GCAATG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 72, 90
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMI GCAATG 1 cut(s) 83
BseMII CTCAG 4 cut(s) 114, 123, 456, 500
BseNI ACTGG 4 cut(s) 55, 131, 282, 463
BseXI GCAGC 2 cut(s) 418, 490
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 1 cut(s) 331
BsiHKAI GWGCWC 2 cut(s) 402, 471
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 277
BsnI GGCC 1 cut(s) 331
BsoBI CYCGRG 1 cut(s) 482
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 402, 471
Bsp143I GATC 3 cut(s) 196, 302, 450
BspACI CCGC 2 cut(s) 80, 161
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 4 cut(s) 113, 124, 457, 501
BspFNI CGCG 1 cut(s) 161
BspHI TCATGA 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 330, 442
BspPI GGATC 2 cut(s) 310, 445
BspT104I TTCGAA 1 cut(s) 336
BsrDI GCAATG 1 cut(s) 83
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 4 cut(s) 55, 131, 282, 463
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 72, 90
BssMI GATC 3 cut(s) 196, 302, 450
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 4 cut(s) 100, 132, 465, 509
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 286
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 199, 305, 453
BstMBI GATC 3 cut(s) 196, 302, 450
BstMWI GCNNNNNNNGC 4 cut(s) 77, 101, 406, 475
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 418, 490
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 2 cut(s) 302, 450
BstYI RGATCY 2 cut(s) 302, 450
BsuRI GGCC 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 286
Cac8I GCNNGC 1 cut(s) 106
CaiI CAGNNNCTG 1 cut(s) 205
CciI TCATGA 1 cut(s) 354
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 25, 355
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 4 cut(s) 100, 132, 465, 509
DpnI GATC 3 cut(s) 198, 304, 452
DpnII GATC 3 cut(s) 196, 302, 450
Ecl136II GAGCTC 2 cut(s) 400, 469
Eco24I GRGCYC 2 cut(s) 402, 471
Eco53kI GAGCTC 2 cut(s) 400, 469
Eco57I CTGAAG 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 482
EcoICRI GAGCTC 2 cut(s) 400, 469
EcoO109I RGGNCCY 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 169
EcoT38I GRGCYC 2 cut(s) 402, 471
FaeI CATG 2 cut(s) 28, 358
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FatI CATG 2 cut(s) 24, 354
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 3 cut(s) 81, 407, 479
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 2 cut(s) 402, 471
Fsp4HI GCNGC 3 cut(s) 81, 407, 479
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 81, 407, 479
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 331
HapII CCGG 1 cut(s) 209
HhaI GCGC 1 cut(s) 161
Hin1II CATG 2 cut(s) 28, 358
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 4 cut(s) 98, 174, 181, 263
HpaII CCGG 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 61, 498
Hpy188III TCNNGA 3 cut(s) 178, 300, 355
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 2 cut(s) 41, 319
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 1 cut(s) 394
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 101, 406, 475
HpyF3I CTNAG 4 cut(s) 100, 132, 465, 509
Hsp92II CATG 2 cut(s) 28, 358
HspAI GCGC 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 3 cut(s) 196, 302, 450
LmnI GCTCC 2 cut(s) 397, 440
Lsp1109I GCAGC 2 cut(s) 418, 490
LweI GCATC 1 cut(s) 271
MaeIII GTNAC 3 cut(s) 9, 163, 368
MalI GATC 3 cut(s) 198, 304, 452
MboI GATC 3 cut(s) 196, 302, 450
MboII GAAGA 3 cut(s) 343, 350, 511
MflI RGATCY 2 cut(s) 302, 450
MhlI GDGCHC 2 cut(s) 402, 471
MlyI GAGTC 2 cut(s) 92, 183
MmeI TCCRAC 1 cut(s) 21
MnlI CCTC 3 cut(s) 100, 281, 359
MseI TTAA 2 cut(s) 233, 423
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 4 cut(s) 77, 101, 406, 475
NdeII GATC 3 cut(s) 196, 302, 450
NlaIII CATG 2 cut(s) 28, 358
NlaIV GGNNCC 2 cut(s) 330, 442
NmeAIII GCCGAG 1 cut(s) 71
NmuCI GTSAC 1 cut(s) 163
NspV TTCGAA 1 cut(s) 336
PagI TCATGA 1 cut(s) 354
PfeI GAWTC 2 cut(s) 181, 263
PfoI TCCNGGA 1 cut(s) 169
PkrI GCNGC 3 cut(s) 82, 408, 480
PleI GAGTC 2 cut(s) 92, 182
PpsI GAGTC 2 cut(s) 92, 182
Psp124BI GAGCTC 2 cut(s) 402, 471
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 330, 442
PspPI GGNCC 1 cut(s) 329
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 2 cut(s) 302, 450
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SacI GAGCTC 2 cut(s) 402, 471
SaqAI TTAA 2 cut(s) 233, 423
SatI GCNGC 3 cut(s) 81, 407, 479
Sau3AI GATC 3 cut(s) 196, 302, 450
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 92, 183
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 2 cut(s) 402, 471
SetI ASST 9 cut(s) 33, 141, 273, 292, 320, 370, 402, 471, 483
SfaNI GCATC 1 cut(s) 271
SfuI TTCGAA 1 cut(s) 336
SsiI CCGC 2 cut(s) 80, 161
SstI GAGCTC 2 cut(s) 402, 471
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 223
TaqI TCGA 1 cut(s) 336
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 181, 263
Tru1I TTAA 2 cut(s) 233, 423
Tru9I TTAA 2 cut(s) 233, 423
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 2 cut(s) 406, 478
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 2 cut(s) 255, 343
TspGWI ACGGA 2 cut(s) 232, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.