MD10G1138700.v1.1

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005697
Physical Location & Seq
Reverse (-)
22371331 .. 22373653
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1138700.v1.1.491

Sequence Viewer

Length: 1872 bp
ATGGCGCTCAATCTGCGGCAGAGGCAAACAGAATGTATTACCCGTATGCTGAATCTGAACCAACCCGTTAATGCAACCGGTATGGCCAACGAGGAGGTCTATAAGATCTTGATCTATGATAAGTTTTGTCAAAACATACTCTCCCCGTTGATCCATGTCAAGGACCTCCGTAAGCACGGCGTGACCCTGTACTTCCTGATTGACAAGGACCGAAAACCTGTTCACGATGTGCCTGCTGTCTACTTTGTCCAGCCCAGCCATTCCAACATTCAGAGGATCATTGCCGACACCTCCAACTCGCTGTATGATAGCTTCCATCTGAACTTCTCGTCCTTGATTCCTCGACCTCTGCTTGAGGACCTGGCATCTGGGACTTTGAATTCAGATTCGATTCATCGGATTTCGAAGGTGCATGATCAGTATTTGGAGTTTGTGACACTGGAAGATAATTTGTTCTCATTGGCACAGAAGAACAGTTATGTTCAGTTAAACGATCCCAAGGCAGGAGACCGTGAGATTGAGGAGATTATTGAAAAGATAGTTGGTGGCTTGTTCTGTGTTTTGGCTACGCTTGCTGTTGTGCCAATTATCAGGTGCCCTCGCGGTGGACCAGCTGAGATGGTAGCATCAGCATTGGATCAGAGACTTCGTGACCACTTGTTGTCAAAAAACAATCTGTTTACCGAAGGTGGAAATTTTGTGACCTCTTTCCAGCGCCCCATATTGTGTATATTTGATCGTAATTTTGAGTTATCTGTGGGAATACAGCATGATTTTAGGTACCGACCCTTGGTGCATGATATTCTTGGATTGAAGCTTAATAGGTTGAATGTTCAAGGTGAAAAGGGTGGGATGAAGTCATATGAGTTAGATAGCTCAGATCCCTTTTGGGTAGCAAATGGGTCTCTAGAATTTCCGGAAGTAGCGGTAGAGATTGAGACCCAATTGAATAAGTATAAGAGGGATGTTGATGAGGTCAACAGACGGACTGGCGGGAATGATGGAGCTGAGTTTGACGGAACAGACATGATTGGGAATACAAAACATTTGATGAGTGCAGTGAACTCATTGCCAGAGTTGACAGCACGGAAGCAGGTGATTGATAAGCACACCAACATTGCAACTGTGTTGTTGGGTGAGATCAAAGAGAGATTGCTCGATTCTTTTGCCAAAAAGGAGTATGACATGATGGTTAGAGGAGGCATTGATCGAAGTGAACTTTTAGGTGTGCTTAGAGGTAAAGGGAGTAAGATGGATAAGCTGCGGTTTTCCATCATGTATCTTATTTCTTCTGAAGCCATTAATCAACAGGAAGTGGAAGCAGTGGAAGCAGCCCTCAGGGAATCTGAGGTTGATACCAGTGCATTTCAGTATGTGAAGAAGATCAAGGCGCTGAATGTCTCACTGGCATCAGCAAATTCTGCTAGCAGAAGCAACATAGTCGATTGGGCAGAGAAACTCTATGGGCAATCAATAAGTGCTGTGACAGCAGGTGTGAAAAATCTTTTGTCCAATGATAGGCAGCTAGCACTGACAAGAACAGTGGAGGCCTTGGCGGAGGGGAGGCCGAATCCAGAAATTGATTCCTACCTTGTGTTTGATCCTCGTGCTCCGAAGTCAAGCTCAGGAATGGGTAGCAGCCATTTAAAAGGACCTTTCAAGGAAGCTATCGTCTTCATGATTGGTGGTGGTAATTATGTGGAGTATGGGAGCCTACAAGAGCTTGCGCAGCGTCAGCAGCCTGTCAAGCATGTTATATATGGAACAACAGAAATACTCACAGGACAGGAGTTTGTGGAGCAGCTTGCAGTATTGGGGCAAAAGATGGGATTGGGTGGTGCTCCTCCTCCTCCACACGCTTCAACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

624

Amino Acids

69.37

Weight (kDa)

6.1

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 35 - 601 4.1e-123 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1084, 1481
Acc16I TGCGCA 1 cut(s) 1728
Acc36I ACCTGC 2 cut(s) 1084, 1481
Acc65I GGTACC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 594, 780
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 603
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 6 cut(s) 16, 603, 926, 993, 1264, 1556
AclWI GGATC 6 cut(s) 145, 284, 488, 645, 875, 1595
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 4 cut(s) 379, 694, 911, 1417
AcuI CTGAAG 1 cut(s) 1314
AdeI CACNNNGTG 2 cut(s) 181, 229
AfaI GTAC 2 cut(s) 191, 782
AfiI CCNNNNNNNGG 5 cut(s) 160, 503, 605, 790, 1518
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 8 cut(s) 379, 533, 814, 829, 836, 949, 1660, 1863
AjnI CCWGG 1 cut(s) 360
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
Alw21I GWGCWC 2 cut(s) 1612, 1843
Alw26I GTCTC 5 cut(s) 501, 637, 909, 932, 1405
AlwI GGATC 6 cut(s) 145, 284, 488, 645, 875, 1595
Aor13HI TCCGGA 1 cut(s) 916
AoxI GGCC 3 cut(s) 84, 1548, 1565
ApeKI GCWGC 7 cut(s) 1261, 1331, 1522, 1638, 1729, 1738, 1801
ApoI RAATTY 4 cut(s) 379, 694, 911, 1417
AseI ATTAAT 1 cut(s) 1302
AsiGI ACCGGT 1 cut(s) 77
Asp718I GGTACC 1 cut(s) 780
AspLEI GCGC 4 cut(s) 7, 717, 1393, 1729
AspS9I GGNCC 5 cut(s) 163, 208, 358, 608, 1652
AsuHPI GGTGA 3 cut(s) 851, 1108, 1148
AsuII TTCGAA 1 cut(s) 404
AsuNHI GCTAGC 2 cut(s) 1424, 1525
AvaII GGWCC 5 cut(s) 163, 208, 358, 608, 1652
AxyI CCTNAGG 1 cut(s) 1337
BaeGI GKGCMC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 86
BanI GGYRCC 2 cut(s) 594, 780
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BauI CACGAG 1 cut(s) 1605
BbsI GAAGAC 1 cut(s) 1666
Bbv12I GWGCWC 2 cut(s) 1612, 1843
BbvI GCAGC 7 cut(s) 1248, 1343, 1534, 1650, 1741, 1750, 1813
BccI CCATC 7 cut(s) 324, 613, 995, 1183, 1246, 1280, 1819
BceAI ACGGC 1 cut(s) 193
BcgI CGANNNNNNTGC 6 cut(s) 215, 249, 1148, 1182, 1423, 1457
BciT130I CCWGG 1 cut(s) 362
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 5 cut(s) 501, 637, 909, 932, 1405
BfaI CTAG 3 cut(s) 908, 1425, 1526
BfoI RGCGCY 3 cut(s) 8, 718, 1394
BfuAI ACCTGC 2 cut(s) 1084, 1481
BglII AGATCT 1 cut(s) 105
BisI GCNGC 8 cut(s) 17, 1262, 1332, 1523, 1639, 1730, 1739, 1802
BlsI GCNGC 8 cut(s) 18, 1263, 1333, 1524, 1640, 1731, 1740, 1803
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 5 cut(s) 163, 208, 358, 608, 1652
BmgT120I GGNCC 5 cut(s) 163, 208, 358, 608, 1652
BmiI GGNNCC 3 cut(s) 596, 782, 1712
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 3 cut(s) 374, 635, 1418
BmtI GCTAGC 2 cut(s) 1428, 1529
BpiI GAAGAC 1 cut(s) 1666
Bpu10I CCTNAGC 1 cut(s) 1624
Bpu14I TTCGAA 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 110
BsaI GGTCTC 3 cut(s) 501, 909, 932
BsaJI CCNNGG 3 cut(s) 498, 789, 1551
BsaWI WCCGGW 2 cut(s) 77, 916
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 503, 605, 790, 1518
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 4 cut(s) 444, 994, 1359, 1410
Bse21I CCTNAGG 1 cut(s) 1337
Bse3DI GCAATG 3 cut(s) 279, 1067, 1116
Bse8I GATNNNNATC 1 cut(s) 110
BseAI TCCGGA 1 cut(s) 916
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 498, 789, 1551
BseGI GGATG 2 cut(s) 858, 970
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 5 cut(s) 160, 503, 605, 790, 1518
BseMI GCAATG 3 cut(s) 279, 1067, 1116
BseMII CTCAG 6 cut(s) 606, 891, 999, 1338, 1351, 1638
BseNI ACTGG 4 cut(s) 444, 994, 1359, 1410
BseRI GAGGAG 6 cut(s) 107, 536, 1212, 1833, 1836, 1839
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 7 cut(s) 1248, 1343, 1534, 1650, 1741, 1750, 1813
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 1077
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 3 cut(s) 86, 1550, 1567
BshNI GGYRCC 2 cut(s) 594, 780
BshTI ACCGGT 1 cut(s) 77
BsiHKAI GWGCWC 2 cut(s) 1612, 1843
BsiSI CCGG 2 cut(s) 78, 917
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 5 cut(s) 160, 503, 605, 790, 1518
BsmAI GTCTC 5 cut(s) 501, 637, 909, 932, 1405
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 3 cut(s) 86, 1550, 1567
Bso31I GGTCTC 3 cut(s) 501, 909, 932
Bsp119I TTCGAA 1 cut(s) 404
Bsp1286I GDGCHC 3 cut(s) 599, 1612, 1843
Bsp13I TCCGGA 1 cut(s) 916
BspACI CCGC 6 cut(s) 16, 603, 926, 993, 1264, 1556
BspANI GGCC 3 cut(s) 86, 1550, 1567
BspCNI CTCAG 6 cut(s) 607, 890, 1000, 1339, 1350, 1637
BspEI TCCGGA 1 cut(s) 916
BspFNI CGCG 1 cut(s) 603
BspHI TCATGA 1 cut(s) 1677
BspLI GGNNCC 3 cut(s) 596, 782, 1712
BspMI ACCTGC 2 cut(s) 1084, 1481
BspOI GCTAGC 2 cut(s) 1428, 1529
BspPI GGATC 6 cut(s) 145, 284, 488, 645, 875, 1595
BspT104I TTCGAA 1 cut(s) 404
BspT107I GGYRCC 2 cut(s) 594, 780
BspTNI GGTCTC 3 cut(s) 501, 909, 932
BsrDI GCAATG 3 cut(s) 279, 1067, 1116
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 4 cut(s) 444, 994, 1359, 1410
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 498, 789, 1551
BssSI CACGAG 1 cut(s) 1605
BssT1I CCWWGG 3 cut(s) 498, 789, 1551
Bst2BI CACGAG 1 cut(s) 1605
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 4 cut(s) 476, 512, 1126, 1543
BstAPI GCANNNNNTGC 1 cut(s) 1421
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 6 cut(s) 234, 573, 1426, 1527, 1725, 1806
BstDEI CTNAG 7 cut(s) 615, 877, 1008, 1232, 1337, 1347, 1624
BstF5I GGATG 2 cut(s) 858, 970
BstFNI CGCG 1 cut(s) 603
BstH2I RGCGCY 3 cut(s) 8, 718, 1394
BstHHI GCGC 4 cut(s) 7, 717, 1393, 1729
BstMAI GTCTC 5 cut(s) 501, 637, 909, 932, 1405
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 1 cut(s) 1754
BstSCI CCNGG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 7 cut(s) 1248, 1343, 1534, 1650, 1741, 1750, 1813
BstV2I GAAGAC 1 cut(s) 1666
BstX2I RGATCY 2 cut(s) 105, 880
BstYI RGATCY 2 cut(s) 105, 880
Bsu36I CCTNAGG 1 cut(s) 1337
BsuRI GGCC 3 cut(s) 86, 1550, 1567
BtsCI GGATG 2 cut(s) 858, 970
BtsI GCAGTG 2 cut(s) 1065, 1329
BtsIMutI CAGTG 7 cut(s) 437, 1065, 1329, 1366, 1403, 1529, 1548
BveI ACCTGC 2 cut(s) 1084, 1481
Cac8I GCNNGC 6 cut(s) 234, 573, 1426, 1527, 1725, 1806
CciI TCATGA 1 cut(s) 1677
CfoI GCGC 4 cut(s) 7, 717, 1393, 1729
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 5 cut(s) 163, 208, 358, 608, 1652
CseI GACGC 1 cut(s) 1721
Csp6I GTAC 2 cut(s) 190, 781
CspAI ACCGGT 1 cut(s) 77
CspCI CAANNNNNGTGG 2 cut(s) 1524, 1559
CviAII CATG 9 cut(s) 155, 413, 770, 797, 1027, 1186, 1276, 1678, 1751
CviQI GTAC 2 cut(s) 190, 781
DdeI CTNAG 7 cut(s) 615, 877, 1008, 1232, 1337, 1347, 1624
DraI TTTAAA 1 cut(s) 1647
DraIII CACNNNGTG 2 cut(s) 181, 229
EaeI YGGCCR 1 cut(s) 84
EciI GGCGGA 1 cut(s) 1571
Eco130I CCWWGG 3 cut(s) 498, 789, 1551
Eco147I AGGCCT 1 cut(s) 1550
Eco31I GGTCTC 3 cut(s) 501, 909, 932
Eco47I GGWCC 5 cut(s) 163, 208, 358, 608, 1652
Eco57I CTGAAG 1 cut(s) 1314
Eco81I CCTNAGG 1 cut(s) 1337
EcoO109I RGGNCCY 3 cut(s) 163, 358, 1652
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 3 cut(s) 498, 789, 1551
ErhI CCWWGG 3 cut(s) 498, 789, 1551
FaeI CATG 9 cut(s) 158, 416, 773, 800, 1030, 1189, 1279, 1681, 1754
FaqI GGGAC 1 cut(s) 385
FatI CATG 9 cut(s) 154, 412, 769, 796, 1026, 1185, 1275, 1677, 1750
FauI CCCGC 1 cut(s) 986
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 8 cut(s) 17, 1262, 1332, 1523, 1639, 1730, 1739, 1802
FokI GGATG 2 cut(s) 865, 977
Fsp4HI GCNGC 8 cut(s) 17, 1262, 1332, 1523, 1639, 1730, 1739, 1802
FspBI CTAG 3 cut(s) 908, 1425, 1526
FspI TGCGCA 1 cut(s) 1728
GlaI GCGC 4 cut(s) 6, 716, 1392, 1728
GluI GCNGC 8 cut(s) 17, 1262, 1332, 1523, 1639, 1730, 1739, 1802
GsaI CCCAGC 1 cut(s) 258
HaeII RGCGCY 3 cut(s) 8, 718, 1394
HaeIII GGCC 3 cut(s) 86, 1550, 1567
HapII CCGG 2 cut(s) 78, 917
HgaI GACGC 1 cut(s) 1721
HhaI GCGC 4 cut(s) 7, 717, 1393, 1729
Hin1II CATG 9 cut(s) 158, 416, 773, 800, 1030, 1189, 1279, 1681, 1754
Hin6I GCGC 4 cut(s) 5, 715, 1391, 1727
HinP1I GCGC 4 cut(s) 5, 715, 1391, 1727
HincII GTYRAC 2 cut(s) 979, 1080
HindII GTYRAC 2 cut(s) 979, 1080
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 8 cut(s) 52, 337, 386, 391, 1160, 1343, 1570, 1583
HpaII CCGG 2 cut(s) 78, 917
HphI GGTGA 3 cut(s) 851, 1108, 1148
Hpy166II GTNNAC 8 cut(s) 223, 241, 608, 681, 979, 1063, 1080, 1217
Hpy188III TCNNGA 9 cut(s) 109, 196, 224, 650, 908, 917, 1574, 1626, 1678
Hpy8I GTNNAC 8 cut(s) 223, 241, 608, 681, 979, 1063, 1080, 1217
HpyAV CCTTC 2 cut(s) 400, 680
HpyCH4III ACNGT 4 cut(s) 476, 512, 1126, 1543
HpyCH4V TGCA 7 cut(s) 74, 412, 796, 1058, 1121, 1364, 1808
HpyF3I CTNAG 7 cut(s) 615, 877, 1008, 1232, 1337, 1347, 1624
Hsp92II CATG 9 cut(s) 158, 416, 773, 800, 1030, 1189, 1279, 1681, 1754
HspAI GCGC 4 cut(s) 5, 715, 1391, 1727
Kpn2I TCCGGA 1 cut(s) 916
KpnI GGTACC 1 cut(s) 784
Ksp22I TGATCA 1 cut(s) 415
LmnI GCTCC 5 cut(s) 1004, 1615, 1710, 1798, 1846
Lsp1109I GCAGC 7 cut(s) 1248, 1343, 1534, 1650, 1741, 1750, 1813
LweI GCATC 3 cut(s) 374, 635, 1418
MaeI CTAG 3 cut(s) 908, 1425, 1526
MaeIII GTNAC 5 cut(s) 181, 433, 650, 700, 1483
MboII GAAGA 6 cut(s) 455, 481, 1281, 1390, 1393, 1666
MfeI CAATTG 1 cut(s) 944
MflI RGATCY 2 cut(s) 105, 880
MhlI GDGCHC 3 cut(s) 599, 1612, 1843
MlsI TGGCCA 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 288, 318
Mox20I TGGCCA 1 cut(s) 86
MroI TCCGGA 1 cut(s) 916
MscI TGGCCA 1 cut(s) 86
MseI TTAA 5 cut(s) 69, 488, 819, 1302, 1646
Msp20I TGGCCA 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 2 cut(s) 78, 917
MspR9I CCNGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 944
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 603
NdeI CATATG 1 cut(s) 862
NheI GCTAGC 2 cut(s) 1424, 1525
NlaIII CATG 9 cut(s) 158, 416, 773, 800, 1030, 1189, 1279, 1681, 1754
NlaIV GGNNCC 3 cut(s) 596, 782, 1712
NmuCI GTSAC 5 cut(s) 181, 433, 650, 700, 1483
NsbI TGCGCA 1 cut(s) 1728
NspI RCATGY 1 cut(s) 1754
NspV TTCGAA 1 cut(s) 404
PagI TCATGA 1 cut(s) 1677
PaqCI CACCTGC 2 cut(s) 1084, 1481
PceI AGGCCT 1 cut(s) 1550
PfeI GAWTC 8 cut(s) 52, 337, 386, 391, 1160, 1343, 1570, 1583
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 8 cut(s) 18, 1263, 1333, 1524, 1640, 1731, 1740, 1803
PpuMI RGGWCCY 3 cut(s) 163, 358, 1652
PshBI ATTAAT 1 cut(s) 1302
Psp5II RGGWCCY 3 cut(s) 163, 358, 1652
Psp6I CCWGG 1 cut(s) 360
PspFI CCCAGC 1 cut(s) 254
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 596, 782, 1712
PspPI GGNCC 5 cut(s) 163, 208, 358, 608, 1652
PspPPI RGGWCCY 3 cut(s) 163, 358, 1652
PsuI RGATCY 2 cut(s) 105, 880
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 191, 782
RsaNI GTAC 2 cut(s) 190, 781
SaqAI TTAA 5 cut(s) 69, 488, 819, 1302, 1646
SatI GCNGC 8 cut(s) 17, 1262, 1332, 1523, 1639, 1730, 1739, 1802
Sau96I GGNCC 5 cut(s) 163, 208, 358, 608, 1652
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 3 cut(s) 599, 1612, 1843
SfaNI GCATC 3 cut(s) 374, 635, 1418
SfuI TTCGAA 1 cut(s) 404
SinI GGWCC 5 cut(s) 163, 208, 358, 608, 1652
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SseBI AGGCCT 1 cut(s) 1550
SsiI CCGC 6 cut(s) 16, 603, 926, 993, 1264, 1556
SspMI CTAG 3 cut(s) 908, 1425, 1526
StuI AGGCCT 1 cut(s) 1550
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 3 cut(s) 498, 789, 1551
TaaI ACNGT 4 cut(s) 476, 512, 1126, 1543
TaqI TCGA 6 cut(s) 343, 389, 404, 1158, 1210, 1443
TaqII GACCGA 1 cut(s) 225
TatI WGTACW 1 cut(s) 189
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 8 cut(s) 52, 337, 386, 391, 1160, 1343, 1570, 1583
Tru1I TTAA 5 cut(s) 69, 488, 819, 1302, 1646
Tru9I TTAA 5 cut(s) 69, 488, 819, 1302, 1646
TscAI CASTG 7 cut(s) 444, 1065, 1329, 1366, 1410, 1536, 1548
TseFI GTSAC 5 cut(s) 181, 433, 650, 700, 1483
TseI GCWGC 7 cut(s) 1261, 1331, 1522, 1638, 1729, 1738, 1801
Tsp45I GTSAC 5 cut(s) 181, 433, 650, 700, 1483
TspDTI ATGAA 3 cut(s) 383, 869, 1666
TspGWI ACGGA 4 cut(s) 158, 1000, 1032, 1102
TspRI CASTG 7 cut(s) 444, 1065, 1329, 1366, 1410, 1536, 1548
VpaK11BI GGWCC 5 cut(s) 163, 208, 358, 608, 1652
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 4 cut(s) 379, 694, 911, 1417
XbaI TCTAGA 1 cut(s) 907
XceI RCATGY 1 cut(s) 1754
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 3 cut(s) 908, 1425, 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.