pycom05g13050

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005697
Physical Location & Seq
Reverse (-)
16621527 .. 16622546
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13050.1

Sequence Viewer

Length: 1020 bp
ATGAAGTCGTATGAGTTAGATAGCTCAGATCCCTTTTGGGTAGCAAATGGGTCTCTAGAATTTCCGGAAGTAGCAGTAGAGATTGAGACCCAATTGAATAAGTATAAGAGGGATGTTGATGAGGTCAATAGACGAACTGGTGGGAATGATGGAGCTGAGTTTGATGGGACAGACATGATTGGGAACACAAAACATTTGATGAGTGCAGTGAACTCATTGCCAGAGTTGACTGCGCGGAAGCAGGTGATTGATAAGCACACCAACATTGCAACTGTGTTGTTGAGTGAGATCAAAGAGAGACTGCTCGATTCTTTTGCTAAAAAGGAGTATGACATGATGGTTAGAGGAGGCATTGATCGCAGCGAACTTTTAGGTGTGCTTAGAGGGAAAGGGAGTAAGATGGATAAGCTGCGGTTTTCAATCATGTATCTTATTTCTTCTGAAACCATTAATCAACAAGAAGTGGAAGCAGTGGAAGCAGCCCTCAGGGAATCTGAGGTTGATACCAGTGCATTTCAGTATGTGAAGAAGATCAAGGCGCTGAATGTCTCACTGGCATCAGCAAATTCTGCTAACAGAAGTAACATAGTCGATTGGGCAGAGAAACTTTATGGGCAATCAATAAGCGCTGTGACAGCAGGTGTGAAAAATCTTTTATCCAATGATAGGCAGCTAGCACTAACAAGAACAGTGGAGGCCTTGGTGGAGGGGAGGCCGAATCCAGAAATTGATTCCTATCTTGTGATCGATCCTCGTGCTCCGAAGTCAAGCTCAGGAATAGGTAGCAGCCATTTAAAAGGACCTTTCAAGGAAGCTATCGTCTTCATGATTGGAGGTGGTAATTATGTGGAGTACGGGAGCCTACAAGAGCTTGCGCAGCGTCAGCAACCTGTCAAGCATGTTATATACGGAACAACAGAAATACTCACAGGTCAGGAGTTTGTGGAGCAGCTCGCAGTACTGGGGCAAAAAATGGGATTGGGTGGTGCTCCTACTCCTCCTCCTGCCTCAACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

340

Amino Acids

37.15

Weight (kDa)

5.45

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 2 - 317 9.2e-52 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 232, 629
Acc16I TGCGCA 1 cut(s) 876
Acc36I ACCTGC 2 cut(s) 232, 629
AccII CGCG 1 cut(s) 235
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 2 cut(s) 235, 412
AclWI GGATC 2 cut(s) 23, 743
AcsI RAATTY 2 cut(s) 59, 565
AfaI GTAC 2 cut(s) 854, 960
AfeI AGCGCT 1 cut(s) 628
AfiI CCNNNNNNNGG 1 cut(s) 666
AgsI TTSAA 3 cut(s) 97, 420, 808
AluBI AGCT 8 cut(s) 24, 155, 409, 673, 771, 815, 871, 952
AluI AGCT 8 cut(s) 24, 155, 409, 673, 771, 815, 871, 952
Alw21I GWGCWC 2 cut(s) 760, 991
Alw26I GTCTC 4 cut(s) 57, 80, 292, 553
AlwI GGATC 2 cut(s) 23, 743
Aor13HI TCCGGA 1 cut(s) 64
Aor51HI AGCGCT 1 cut(s) 628
AoxI GGCC 2 cut(s) 696, 713
ApeKI GCWGC 7 cut(s) 360, 409, 479, 670, 786, 877, 949
ApoI RAATTY 2 cut(s) 59, 565
AseI ATTAAT 1 cut(s) 450
AspLEI GCGC 4 cut(s) 235, 541, 629, 877
AspS9I GGNCC 1 cut(s) 800
AsuHPI GGTGA 1 cut(s) 256
AsuNHI GCTAGC 1 cut(s) 673
AvaII GGWCC 1 cut(s) 800
AxyI CCTNAGG 1 cut(s) 485
BauI CACGAG 1 cut(s) 753
BbsI GAAGAC 1 cut(s) 814
Bbv12I GWGCWC 2 cut(s) 760, 991
BbvI GCAGC 7 cut(s) 372, 396, 491, 682, 798, 889, 961
BccI CCATC 4 cut(s) 143, 158, 331, 394
BcgI CGANNNNNNTGC 4 cut(s) 296, 330, 737, 771
BcoDI GTCTC 4 cut(s) 57, 80, 292, 553
BfaI CTAG 2 cut(s) 56, 674
BfoI RGCGCY 2 cut(s) 542, 630
BfuAI ACCTGC 2 cut(s) 232, 629
BisI GCNGC 7 cut(s) 361, 410, 480, 671, 787, 878, 950
BlsI GCNGC 7 cut(s) 362, 411, 481, 672, 788, 879, 951
BmcAI AGTACT 1 cut(s) 960
Bme18I GGWCC 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 1 cut(s) 860
BmrI ACTGGG 1 cut(s) 971
BmsI GCATC 1 cut(s) 566
BmtI GCTAGC 1 cut(s) 677
BmuI ACTGGG 1 cut(s) 971
BpiI GAAGAC 1 cut(s) 814
Bpu10I CCTNAGC 1 cut(s) 772
Bsa29I ATCGAT 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 735
BsaI GGTCTC 2 cut(s) 57, 80
BsaJI CCNNGG 1 cut(s) 699
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 985, 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 666
Bse1I ACTGG 4 cut(s) 142, 507, 558, 966
Bse21I CCTNAGG 1 cut(s) 485
Bse3DI GCAATG 2 cut(s) 215, 264
Bse8I GATNNNNATC 1 cut(s) 735
BseAI TCCGGA 1 cut(s) 64
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 1 cut(s) 699
BseGI GGATG 1 cut(s) 118
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 1 cut(s) 666
BseMI GCAATG 2 cut(s) 215, 264
BseMII CTCAG 5 cut(s) 39, 147, 486, 499, 786
BseNI ACTGG 4 cut(s) 142, 507, 558, 966
BseRI GAGGAG 3 cut(s) 360, 987, 990
BseXI GCAGC 7 cut(s) 372, 396, 491, 682, 798, 889, 961
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 2 cut(s) 698, 715
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 2 cut(s) 760, 991
BsiSI CCGG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 666
BsmAI GTCTC 4 cut(s) 57, 80, 292, 553
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 2 cut(s) 698, 715
Bso31I GGTCTC 2 cut(s) 57, 80
Bsp1286I GDGCHC 2 cut(s) 760, 991
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 6 cut(s) 28, 288, 355, 531, 744, 748
BspACI CCGC 2 cut(s) 235, 412
BspANI GGCC 2 cut(s) 698, 715
BspCNI CTCAG 5 cut(s) 38, 148, 487, 498, 785
BspDI ATCGAT 1 cut(s) 747
BspEI TCCGGA 1 cut(s) 64
BspFNI CGCG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 1 cut(s) 860
BspMI ACCTGC 2 cut(s) 232, 629
BspOI GCTAGC 1 cut(s) 677
BspPI GGATC 2 cut(s) 23, 743
BspTNI GGTCTC 2 cut(s) 57, 80
BsrDI GCAATG 2 cut(s) 215, 264
BsrI ACTGG 4 cut(s) 142, 507, 558, 966
BssECI CCNNGG 1 cut(s) 699
BssMI GATC 6 cut(s) 28, 288, 355, 531, 744, 748
BssSI CACGAG 1 cut(s) 753
BssT1I CCWWGG 1 cut(s) 699
Bst2BI CACGAG 1 cut(s) 753
Bst4CI ACNGT 2 cut(s) 274, 691
BstAPI GCANNNNNTGC 1 cut(s) 569
BstC8I GCNNGC 3 cut(s) 675, 873, 954
BstDEI CTNAG 6 cut(s) 25, 156, 380, 485, 495, 772
BstF5I GGATG 1 cut(s) 118
BstFNI CGCG 1 cut(s) 235
BstH2I RGCGCY 2 cut(s) 542, 630
BstHHI GCGC 4 cut(s) 235, 541, 629, 877
BstKTI GATC 6 cut(s) 31, 291, 358, 534, 747, 751
BstMAI GTCTC 4 cut(s) 57, 80, 292, 553
BstMBI GATC 6 cut(s) 28, 288, 355, 531, 744, 748
BstMWI GCNNNNNNNGC 6 cut(s) 357, 476, 569, 635, 877, 883
BstNSI RCATGY 1 cut(s) 902
BstUI CGCG 1 cut(s) 235
BstV1I GCAGC 7 cut(s) 372, 396, 491, 682, 798, 889, 961
BstV2I GAAGAC 1 cut(s) 814
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
Bsu15I ATCGAT 1 cut(s) 747
Bsu36I CCTNAGG 1 cut(s) 485
BsuRI GGCC 2 cut(s) 698, 715
BsuTUI ATCGAT 1 cut(s) 747
BtsCI GGATG 1 cut(s) 118
BtsI GCAGTG 2 cut(s) 213, 477
BtsIMutI CAGTG 5 cut(s) 213, 477, 514, 551, 696
BveI ACCTGC 2 cut(s) 232, 629
Cac8I GCNNGC 3 cut(s) 675, 873, 954
CciI TCATGA 1 cut(s) 825
CfoI GCGC 4 cut(s) 235, 541, 629, 877
Cfr13I GGNCC 1 cut(s) 800
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 869
Csp6I GTAC 2 cut(s) 853, 959
CspCI CAANNNNNGTGG 2 cut(s) 672, 707
CviAII CATG 5 cut(s) 175, 334, 424, 826, 899
CviQI GTAC 2 cut(s) 853, 959
DdeI CTNAG 6 cut(s) 25, 156, 380, 485, 495, 772
DpnI GATC 6 cut(s) 30, 290, 357, 533, 746, 750
DpnII GATC 6 cut(s) 28, 288, 355, 531, 744, 748
DraI TTTAAA 1 cut(s) 795
Eco130I CCWWGG 1 cut(s) 699
Eco147I AGGCCT 1 cut(s) 698
Eco31I GGTCTC 2 cut(s) 57, 80
Eco47I GGWCC 1 cut(s) 800
Eco47III AGCGCT 1 cut(s) 628
Eco81I CCTNAGG 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 800
EcoT14I CCWWGG 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 5 cut(s) 178, 337, 427, 829, 902
FaqI GGGAC 1 cut(s) 181
FatI CATG 5 cut(s) 174, 333, 423, 825, 898
Fnu4HI GCNGC 7 cut(s) 361, 410, 480, 671, 787, 878, 950
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 7 cut(s) 361, 410, 480, 671, 787, 878, 950
FspBI CTAG 2 cut(s) 56, 674
FspI TGCGCA 1 cut(s) 876
GlaI GCGC 4 cut(s) 234, 540, 628, 876
GluI GCNGC 7 cut(s) 361, 410, 480, 671, 787, 878, 950
HaeII RGCGCY 2 cut(s) 542, 630
HaeIII GGCC 2 cut(s) 698, 715
HapII CCGG 1 cut(s) 65
HgaI GACGC 1 cut(s) 869
HhaI GCGC 4 cut(s) 235, 541, 629, 877
Hin1II CATG 5 cut(s) 178, 337, 427, 829, 902
Hin6I GCGC 4 cut(s) 233, 539, 627, 875
HinP1I GCGC 4 cut(s) 233, 539, 627, 875
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 4 cut(s) 308, 491, 718, 731
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 2 cut(s) 211, 228
Hpy188I TCNGA 4 cut(s) 28, 442, 496, 762
Hpy188III TCNNGA 6 cut(s) 56, 65, 722, 774, 826, 935
Hpy8I GTNNAC 2 cut(s) 211, 228
HpyCH4III ACNGT 2 cut(s) 274, 691
HpyCH4V TGCA 3 cut(s) 206, 269, 512
HpyF10VI GCNNNNNNNGC 6 cut(s) 357, 476, 569, 635, 877, 883
HpyF3I CTNAG 6 cut(s) 25, 156, 380, 485, 495, 772
Hsp92II CATG 5 cut(s) 178, 337, 427, 829, 902
HspAI GCGC 4 cut(s) 233, 539, 627, 875
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 6 cut(s) 28, 288, 355, 531, 744, 748
LmnI GCTCC 5 cut(s) 152, 763, 858, 946, 994
Lsp1109I GCAGC 7 cut(s) 372, 396, 491, 682, 798, 889, 961
LweI GCATC 1 cut(s) 566
MaeI CTAG 2 cut(s) 56, 674
MaeIII GTNAC 2 cut(s) 581, 631
MalI GATC 6 cut(s) 30, 290, 357, 533, 746, 750
MboI GATC 6 cut(s) 28, 288, 355, 531, 744, 748
MboII GAAGA 4 cut(s) 429, 538, 541, 814
MfeI CAATTG 1 cut(s) 92
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 2 cut(s) 760, 991
MluCI AATT 5 cut(s) 59, 92, 565, 726, 841
MroI TCCGGA 1 cut(s) 64
MseI TTAA 2 cut(s) 450, 794
MspI CCGG 1 cut(s) 65
MunI CAATTG 1 cut(s) 92
MvnI CGCG 1 cut(s) 235
MwoI GCNNNNNNNGC 6 cut(s) 357, 476, 569, 635, 877, 883
NdeII GATC 6 cut(s) 28, 288, 355, 531, 744, 748
NheI GCTAGC 1 cut(s) 673
NlaIII CATG 5 cut(s) 178, 337, 427, 829, 902
NlaIV GGNNCC 1 cut(s) 860
NmuCI GTSAC 1 cut(s) 631
NsbI TGCGCA 1 cut(s) 876
NspI RCATGY 1 cut(s) 902
PagI TCATGA 1 cut(s) 825
PaqCI CACCTGC 2 cut(s) 232, 629
PceI AGGCCT 1 cut(s) 698
PfeI GAWTC 4 cut(s) 308, 491, 718, 731
PkrI GCNGC 7 cut(s) 362, 411, 481, 672, 788, 879, 951
PpuMI RGGWCCY 1 cut(s) 800
PshBI ATTAAT 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 800
PspN4I GGNNCC 1 cut(s) 860
PspPI GGNCC 1 cut(s) 800
PspPPI RGGWCCY 1 cut(s) 800
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 2 cut(s) 854, 960
RsaNI GTAC 2 cut(s) 853, 959
SaqAI TTAA 2 cut(s) 450, 794
SatI GCNGC 7 cut(s) 361, 410, 480, 671, 787, 878, 950
Sau3AI GATC 6 cut(s) 28, 288, 355, 531, 744, 748
Sau96I GGNCC 1 cut(s) 800
ScaI AGTACT 1 cut(s) 960
SduI GDGCHC 2 cut(s) 760, 991
SfaNI GCATC 1 cut(s) 566
SinI GGWCC 1 cut(s) 800
Sse9I AATT 5 cut(s) 59, 92, 565, 726, 841
SseBI AGGCCT 1 cut(s) 698
SsiI CCGC 2 cut(s) 235, 412
SspMI CTAG 2 cut(s) 56, 674
StuI AGGCCT 1 cut(s) 698
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 2 cut(s) 274, 691
TaqI TCGA 3 cut(s) 306, 591, 747
TasI AATT 5 cut(s) 59, 92, 565, 726, 841
TatI WGTACW 1 cut(s) 958
TfiI GAWTC 4 cut(s) 308, 491, 718, 731
Tru1I TTAA 2 cut(s) 450, 794
Tru9I TTAA 2 cut(s) 450, 794
TscAI CASTG 5 cut(s) 213, 477, 514, 558, 696
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 7 cut(s) 360, 409, 479, 670, 786, 877, 949
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 2 cut(s) 17, 814
TspGWI ACGGA 1 cut(s) 924
TspRI CASTG 5 cut(s) 213, 477, 514, 558, 696
VpaK11BI GGWCC 1 cut(s) 800
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 2 cut(s) 59, 565
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 902
XspI CTAG 2 cut(s) 56, 674
ZrmI AGTACT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.