MD05G1133700.v1.1

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005697
Physical Location & Seq
Reverse (-)
26240885 .. 26243224
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1133700.v1.1.491

Sequence Viewer

Length: 1872 bp
ATGGCGCTTAATCTGCGGCAGAGGCAAACAGAATGTATTACCCGCATGCTGAATCTGAACCAACCCGTTAATGCAACCGGTATGGCCAATGAAGAGGTCTATAAGATCTTGATCTATGATAAGTTTTGCCAAAACATACTCTCCCCGTTGATCCATGTCAAGGACCTTCGTAAGCACGGTGTGACCCTGTACTTCCTGATTGACAAGGACCGAAAACCCGTTCATGATGTGCCTGCTGTCTACTTTGTCCAACCCAGCCATTCCAACATTCAGAGGATCATTGCTGACACTTCGAACTCGCTGTATGATAGCTTCCATCTGAACTTCTCGTCCTTGATTCCCCGCCCACTGCTCGAGGATCTGGCATCTGGGACTTTGAATTCAGATTCGATTCATCGGATTTCAAAGGTGCATGATCAGTATTTGGAGTTTGTGACACTGGAAGATAATTTGTTCTCGTTGGCACAGAAGAACACTTATGTTCAGTTAAATGATCCCAAGGCAGGGGACCGTGAGATTGAGGAGATTATTGAAAAGATAGTTGGTGGCTTGTTCTGCGTTTTGGCTACTCTTGCTGTTGTGCCAATTATCAGGTGCCCTCGTGGTGGTCCAGCTGAGATGGTAGCATCAGCATTGGATCAGAGACTTCGTGACCACTTGTTGTCGAAGAACAATCTGTTTACTGAAGGTGGAAACTTTGCGAGCTCTTTCCAGCGCCCCATATTGTGTATATTTGACCGTAATTTTGAGTTATCTGTGGGAATACAGCATGATTTTAGGTACCGGCCCTTGGTGCATGATATTCTTGGATTGAAGCTTAATAGGTTGAATGTTCAAGGTGAAAAGGGTGGGATGAAGTCGTATGAGTTAGATAGCTCAGATCCCTTTTGGGTAGCAAATGGGTCTCTAGAATTTCCGGAAGTAGCAGTAGAGATTGAGACCCAATTGAATAAGTATAAGAGGGATGTTGATGAGGTCAATAGACGAACTGGTGGGAATGATGGAGCTGAATTTGATGGGACAGACATGATTGGGAACACAAAACATTTGATGAGTGCAGTGAACTCATTGCCAGAGTTGACTGCGCGGAAGCAGGTGATTGATAAGCACACCAACATTGCAACTGTGTTGTTGAGTGAGATCAAAGAGAGACTGCTCGATTCTTTTGCTAAAAAGGAGTATGACATGATGGTTAGAGGAGGCATTGATCGCAGCGAACTTTTAGGTGTGCTTAGAGGGAAAGGGAGTAAGATGGATAAGCTGCGGTTTTCTATCATGTACCTTATTTCTTCTGAAACCATTAATCAACAAGAAGTGGAAGCAGTGGAAGCAGCCCTCAGGGAATCTGAGGTTGATACCAGTGCATTTCAGTATGTGAAGAAGATCAAGGCGCTGAATGTCTCACTGGCATCAGCAAATTCTGCTAGCAGAAGTAACATAGTTGATTGGGCAGAGAAACTTTATGGGCAATCAATAAGCGCCGTGACAGCAGGTGTGAAAAATCTTTTATCCAATGATAGGCAGCTAGCACTAACAAGAACAGTGGAGGCCTTGGTGGAGGGGAGGCCGAATCCAGAAATTGATTCCTATCTTGTGTTTGATCCTCGTGCTCCGAAGTCAAGCTCAGGAATAGGTAGCAGCCATTTAAAAGGACCTTTCAAGGAAGCTATCGTCTTCATGATTGGAGGTGGTAATTATGTGGAGTACGGGAGCCTACAAGAGCTTGCGCAGCGTCAGCAACCTGTCAAGCATGTTATATACGGAACAACAGAAATACTCACAGGTCAGGAGTTTGTGGAGCAGCTCGCAGTACTGGGGCAAAAAATGGGATTGGGTGGTGCTCCTACTCCTCCTCCTGCTTCAACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

624

Amino Acids

69.38

Weight (kDa)

6.06

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 35 - 601 7.8e-124 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1084, 1481
Acc16I TGCGCA 1 cut(s) 1728
Acc36I ACCTGC 2 cut(s) 1084, 1481
Acc65I GGTACC 1 cut(s) 780
AccB1I GGYRCC 2 cut(s) 594, 780
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 1087
AccIII TCCGGA 1 cut(s) 916
AciI CCGC 5 cut(s) 16, 43, 343, 1087, 1264
AclWI GGATC 7 cut(s) 145, 284, 366, 488, 645, 875, 1595
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 4 cut(s) 379, 911, 1010, 1417
AcuI CTGAAG 1 cut(s) 705
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 5 cut(s) 191, 782, 1280, 1706, 1812
AfiI CCNNNNNNNGG 6 cut(s) 160, 503, 504, 605, 790, 1518
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 9 cut(s) 379, 405, 533, 814, 829, 836, 949, 1660, 1863
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
Alw21I GWGCWC 3 cut(s) 707, 1612, 1843
Alw26I GTCTC 5 cut(s) 637, 909, 932, 1144, 1405
AlwI GGATC 7 cut(s) 145, 284, 366, 488, 645, 875, 1595
Ama87I CYCGRG 1 cut(s) 353
Aor13HI TCCGGA 1 cut(s) 916
AoxI GGCC 4 cut(s) 84, 785, 1548, 1565
ApeKI GCWGC 7 cut(s) 1212, 1261, 1331, 1522, 1638, 1729, 1801
ApoI RAATTY 4 cut(s) 379, 911, 1010, 1417
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
AseI ATTAAT 1 cut(s) 1302
AsiGI ACCGGT 1 cut(s) 77
Asp718I GGTACC 1 cut(s) 780
AspLEI GCGC 6 cut(s) 7, 717, 1087, 1393, 1481, 1729
AspS9I GGNCC 6 cut(s) 163, 208, 508, 608, 786, 1652
AsuHPI GGTGA 2 cut(s) 851, 1108
AsuII TTCGAA 1 cut(s) 293
AsuNHI GCTAGC 2 cut(s) 1424, 1525
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 5 cut(s) 163, 208, 508, 608, 1652
AxyI CCTNAGG 1 cut(s) 1337
BaeGI GKGCMC 1 cut(s) 599
BalI TGGCCA 1 cut(s) 86
BanI GGYRCC 2 cut(s) 594, 780
BanII GRGCYC 1 cut(s) 707
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BauI CACGAG 2 cut(s) 600, 1605
BbsI GAAGAC 1 cut(s) 1666
Bbv12I GWGCWC 3 cut(s) 707, 1612, 1843
BbvI GCAGC 7 cut(s) 1224, 1248, 1343, 1534, 1650, 1741, 1813
BccI CCATC 6 cut(s) 324, 613, 995, 1010, 1183, 1246
BceAI ACGGC 1 cut(s) 1466
BcgI CGANNNNNNTGC 2 cut(s) 1148, 1182
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 5 cut(s) 637, 909, 932, 1144, 1405
BfaI CTAG 3 cut(s) 908, 1425, 1526
BfoI RGCGCY 4 cut(s) 8, 718, 1394, 1482
BfuAI ACCTGC 2 cut(s) 1084, 1481
BglII AGATCT 1 cut(s) 105
BisI GCNGC 8 cut(s) 17, 1213, 1262, 1332, 1523, 1639, 1730, 1802
BlsI GCNGC 8 cut(s) 18, 1214, 1263, 1333, 1524, 1640, 1731, 1803
BmcAI AGTACT 1 cut(s) 1812
Bme18I GGWCC 5 cut(s) 163, 208, 508, 608, 1652
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 6 cut(s) 163, 208, 508, 608, 786, 1652
BmiI GGNNCC 4 cut(s) 509, 596, 782, 1712
BmrI ACTGGG 1 cut(s) 1823
BmsI GCATC 3 cut(s) 374, 635, 1418
BmtI GCTAGC 2 cut(s) 1428, 1529
BmuI ACTGGG 1 cut(s) 1823
BpiI GAAGAC 1 cut(s) 1666
Bpu10I CCTNAGC 1 cut(s) 1624
Bpu14I TTCGAA 1 cut(s) 293
BsaBI GATNNNNATC 2 cut(s) 110, 1587
BsaI GGTCTC 2 cut(s) 909, 932
BsaJI CCNNGG 3 cut(s) 498, 789, 1551
BsaWI WCCGGW 2 cut(s) 77, 916
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 1837, 1867
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 503, 504, 605, 790, 1518
Bse118I RCCGGY 2 cut(s) 77, 783
Bse1I ACTGG 5 cut(s) 444, 994, 1359, 1410, 1818
Bse21I CCTNAGG 1 cut(s) 1337
Bse3DI GCAATG 3 cut(s) 279, 1067, 1116
Bse8I GATNNNNATC 2 cut(s) 110, 1587
BseAI TCCGGA 1 cut(s) 916
BseDI CCNNGG 3 cut(s) 498, 789, 1551
BseGI GGATG 2 cut(s) 858, 970
BseJI GATNNNNATC 2 cut(s) 110, 1587
BseLI CCNNNNNNNGG 6 cut(s) 160, 503, 504, 605, 790, 1518
BseMI GCAATG 3 cut(s) 279, 1067, 1116
BseMII CTCAG 5 cut(s) 606, 891, 1338, 1351, 1638
BseNI ACTGG 5 cut(s) 444, 994, 1359, 1410, 1818
BseRI GAGGAG 4 cut(s) 536, 1212, 1839, 1842
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 7 cut(s) 1224, 1248, 1343, 1534, 1650, 1741, 1813
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 1077
Bsh1236I CGCG 1 cut(s) 1087
BshFI GGCC 4 cut(s) 86, 787, 1550, 1567
BshNI GGYRCC 2 cut(s) 594, 780
BshTI ACCGGT 1 cut(s) 77
BsiHKAI GWGCWC 3 cut(s) 707, 1612, 1843
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 3 cut(s) 78, 784, 917
BslFI GGGAC 3 cut(s) 385, 521, 1033
BslI CCNNNNNNNGG 6 cut(s) 160, 503, 504, 605, 790, 1518
BsmAI GTCTC 5 cut(s) 637, 909, 932, 1144, 1405
BsmFI GGGAC 3 cut(s) 385, 521, 1033
BsnI GGCC 4 cut(s) 86, 787, 1550, 1567
Bso31I GGTCTC 2 cut(s) 909, 932
BsoBI CYCGRG 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 293
Bsp1286I GDGCHC 4 cut(s) 599, 707, 1612, 1843
Bsp13I TCCGGA 1 cut(s) 916
BspACI CCGC 5 cut(s) 16, 43, 343, 1087, 1264
BspANI GGCC 4 cut(s) 86, 787, 1550, 1567
BspCNI CTCAG 5 cut(s) 607, 890, 1339, 1350, 1637
BspEI TCCGGA 1 cut(s) 916
BspFNI CGCG 1 cut(s) 1087
BspHI TCATGA 2 cut(s) 223, 1677
BspLI GGNNCC 4 cut(s) 509, 596, 782, 1712
BspMI ACCTGC 2 cut(s) 1084, 1481
BspOI GCTAGC 2 cut(s) 1428, 1529
BspPI GGATC 7 cut(s) 145, 284, 366, 488, 645, 875, 1595
BspT104I TTCGAA 1 cut(s) 293
BspT107I GGYRCC 2 cut(s) 594, 780
BspTNI GGTCTC 2 cut(s) 909, 932
BsrDI GCAATG 3 cut(s) 279, 1067, 1116
BsrFI RCCGGY 2 cut(s) 77, 783
BsrI ACTGG 5 cut(s) 444, 994, 1359, 1410, 1818
BssAI RCCGGY 2 cut(s) 77, 783
BssECI CCNNGG 3 cut(s) 498, 789, 1551
BssSI CACGAG 2 cut(s) 600, 1605
BssT1I CCWWGG 3 cut(s) 498, 789, 1551
Bst2BI CACGAG 2 cut(s) 600, 1605
Bst4CI ACNGT 5 cut(s) 179, 512, 740, 1126, 1543
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 1421
BstBI TTCGAA 1 cut(s) 293
BstC8I GCNNGC 7 cut(s) 47, 234, 703, 1426, 1527, 1725, 1806
BstDEI CTNAG 6 cut(s) 615, 877, 1232, 1337, 1347, 1624
BstF5I GGATG 2 cut(s) 858, 970
BstFNI CGCG 1 cut(s) 1087
BstH2I RGCGCY 4 cut(s) 8, 718, 1394, 1482
BstHHI GCGC 6 cut(s) 7, 717, 1087, 1393, 1481, 1729
BstMAI GTCTC 5 cut(s) 637, 909, 932, 1144, 1405
BstNSI RCATGY 2 cut(s) 49, 1754
BstSLI GKGCMC 1 cut(s) 599
BstUI CGCG 1 cut(s) 1087
BstV1I GCAGC 7 cut(s) 1224, 1248, 1343, 1534, 1650, 1741, 1813
BstV2I GAAGAC 1 cut(s) 1666
BstX2I RGATCY 3 cut(s) 105, 358, 880
BstYI RGATCY 3 cut(s) 105, 358, 880
Bsu36I CCTNAGG 1 cut(s) 1337
BsuRI GGCC 4 cut(s) 86, 787, 1550, 1567
BtsCI GGATG 2 cut(s) 858, 970
BtsI GCAGTG 3 cut(s) 347, 1065, 1329
BtsIMutI CAGTG 7 cut(s) 347, 437, 1065, 1329, 1366, 1403, 1548
BveI ACCTGC 2 cut(s) 1084, 1481
Cac8I GCNNGC 7 cut(s) 47, 234, 703, 1426, 1527, 1725, 1806
CciI TCATGA 2 cut(s) 223, 1677
CfoI GCGC 6 cut(s) 7, 717, 1087, 1393, 1481, 1729
Cfr10I RCCGGY 2 cut(s) 77, 783
Cfr13I GGNCC 6 cut(s) 163, 208, 508, 608, 786, 1652
CseI GACGC 1 cut(s) 1721
Csp6I GTAC 5 cut(s) 190, 781, 1279, 1705, 1811
CspAI ACCGGT 1 cut(s) 77
CspCI CAANNNNNGTGG 2 cut(s) 1524, 1559
CviQI GTAC 5 cut(s) 190, 781, 1279, 1705, 1811
DdeI CTNAG 6 cut(s) 615, 877, 1232, 1337, 1347, 1624
DraI TTTAAA 1 cut(s) 1647
DraIII CACNNNGTG 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 705
Eco130I CCWWGG 3 cut(s) 498, 789, 1551
Eco147I AGGCCT 1 cut(s) 1550
Eco24I GRGCYC 1 cut(s) 707
Eco31I GGTCTC 2 cut(s) 909, 932
Eco47I GGWCC 5 cut(s) 163, 208, 508, 608, 1652
Eco53kI GAGCTC 1 cut(s) 705
Eco57I CTGAAG 1 cut(s) 705
Eco81I CCTNAGG 1 cut(s) 1337
Eco88I CYCGRG 1 cut(s) 353
EcoICRI GAGCTC 1 cut(s) 705
EcoO109I RGGNCCY 2 cut(s) 163, 1652
EcoRI GAATTC 1 cut(s) 379
EcoT14I CCWWGG 3 cut(s) 498, 789, 1551
EcoT38I GRGCYC 1 cut(s) 707
ErhI CCWWGG 3 cut(s) 498, 789, 1551
FaqI GGGAC 3 cut(s) 385, 521, 1033
FauI CCCGC 2 cut(s) 50, 350
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 8 cut(s) 17, 1213, 1262, 1332, 1523, 1639, 1730, 1802
FokI GGATG 2 cut(s) 865, 977
FriOI GRGCYC 1 cut(s) 707
Fsp4HI GCNGC 8 cut(s) 17, 1213, 1262, 1332, 1523, 1639, 1730, 1802
FspBI CTAG 3 cut(s) 908, 1425, 1526
FspI TGCGCA 1 cut(s) 1728
GlaI GCGC 6 cut(s) 6, 716, 1086, 1392, 1480, 1728
GluI GCNGC 8 cut(s) 17, 1213, 1262, 1332, 1523, 1639, 1730, 1802
GsaI CCCAGC 1 cut(s) 258
HaeII RGCGCY 4 cut(s) 8, 718, 1394, 1482
HaeIII GGCC 4 cut(s) 86, 787, 1550, 1567
HapII CCGG 3 cut(s) 78, 784, 917
HgaI GACGC 1 cut(s) 1721
HhaI GCGC 6 cut(s) 7, 717, 1087, 1393, 1481, 1729
Hin6I GCGC 6 cut(s) 5, 715, 1085, 1391, 1479, 1727
HinP1I GCGC 6 cut(s) 5, 715, 1085, 1391, 1479, 1727
HincII GTYRAC 1 cut(s) 1080
HindII GTYRAC 1 cut(s) 1080
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 8 cut(s) 52, 337, 386, 391, 1160, 1343, 1570, 1583
HpaII CCGG 3 cut(s) 78, 784, 917
HphI GGTGA 2 cut(s) 851, 1108
Hpy166II GTNNAC 4 cut(s) 241, 681, 1063, 1080
Hpy8I GTNNAC 4 cut(s) 241, 681, 1063, 1080
HpyAV CCTTC 2 cut(s) 176, 680
HpyCH4III ACNGT 5 cut(s) 179, 512, 740, 1126, 1543
HpyCH4V TGCA 6 cut(s) 74, 412, 796, 1058, 1121, 1364
HpyF3I CTNAG 6 cut(s) 615, 877, 1232, 1337, 1347, 1624
HspAI GCGC 6 cut(s) 5, 715, 1085, 1391, 1479, 1727
Kpn2I TCCGGA 1 cut(s) 916
KpnI GGTACC 1 cut(s) 784
Ksp22I TGATCA 1 cut(s) 415
LmnI GCTCC 5 cut(s) 1004, 1615, 1710, 1798, 1846
Lsp1109I GCAGC 7 cut(s) 1224, 1248, 1343, 1534, 1650, 1741, 1813
LweI GCATC 3 cut(s) 374, 635, 1418
MaeI CTAG 3 cut(s) 908, 1425, 1526
MaeIII GTNAC 5 cut(s) 181, 433, 650, 1433, 1483
MboII GAAGA 8 cut(s) 104, 455, 481, 679, 1281, 1390, 1393, 1666
MfeI CAATTG 1 cut(s) 944
MflI RGATCY 3 cut(s) 105, 358, 880
MhlI GDGCHC 4 cut(s) 599, 707, 1612, 1843
MlsI TGGCCA 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 274, 288
Mox20I TGGCCA 1 cut(s) 86
MroI TCCGGA 1 cut(s) 916
MscI TGGCCA 1 cut(s) 86
MseI TTAA 6 cut(s) 9, 69, 488, 819, 1302, 1646
Msp20I TGGCCA 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 3 cut(s) 78, 784, 917
MunI CAATTG 1 cut(s) 944
MvnI CGCG 1 cut(s) 1087
NheI GCTAGC 2 cut(s) 1424, 1525
NlaIV GGNNCC 4 cut(s) 509, 596, 782, 1712
NmuCI GTSAC 4 cut(s) 181, 433, 650, 1483
NsbI TGCGCA 1 cut(s) 1728
NspI RCATGY 2 cut(s) 49, 1754
NspV TTCGAA 1 cut(s) 293
PaeI GCATGC 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 353
PagI TCATGA 2 cut(s) 223, 1677
PaqCI CACCTGC 2 cut(s) 1084, 1481
PceI AGGCCT 1 cut(s) 1550
PfeI GAWTC 8 cut(s) 52, 337, 386, 391, 1160, 1343, 1570, 1583
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 8 cut(s) 18, 1214, 1263, 1333, 1524, 1640, 1731, 1803
PpuMI RGGWCCY 2 cut(s) 163, 1652
PshBI ATTAAT 1 cut(s) 1302
Psp124BI GAGCTC 1 cut(s) 707
Psp5II RGGWCCY 2 cut(s) 163, 1652
PspFI CCCAGC 1 cut(s) 254
PspN4I GGNNCC 4 cut(s) 509, 596, 782, 1712
PspPI GGNCC 6 cut(s) 163, 208, 508, 608, 786, 1652
PspPPI RGGWCCY 2 cut(s) 163, 1652
PspXI VCTCGAGB 1 cut(s) 353
PsuI RGATCY 3 cut(s) 105, 358, 880
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 5 cut(s) 191, 782, 1280, 1706, 1812
RsaNI GTAC 5 cut(s) 190, 781, 1279, 1705, 1811
SacI GAGCTC 1 cut(s) 707
SaqAI TTAA 6 cut(s) 9, 69, 488, 819, 1302, 1646
SatI GCNGC 8 cut(s) 17, 1213, 1262, 1332, 1523, 1639, 1730, 1802
Sau96I GGNCC 6 cut(s) 163, 208, 508, 608, 786, 1652
ScaI AGTACT 1 cut(s) 1812
SduI GDGCHC 4 cut(s) 599, 707, 1612, 1843
SfaNI GCATC 3 cut(s) 374, 635, 1418
Sfr274I CTCGAG 1 cut(s) 353
SfuI TTCGAA 1 cut(s) 293
SinI GGWCC 5 cut(s) 163, 208, 508, 608, 1652
SlaI CTCGAG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 49
SseBI AGGCCT 1 cut(s) 1550
SsiI CCGC 5 cut(s) 16, 43, 343, 1087, 1264
SspMI CTAG 3 cut(s) 908, 1425, 1526
SstI GAGCTC 1 cut(s) 707
StuI AGGCCT 1 cut(s) 1550
StyI CCWWGG 3 cut(s) 498, 789, 1551
TaaI ACNGT 5 cut(s) 179, 512, 740, 1126, 1543
TaqI TCGA 5 cut(s) 293, 354, 389, 665, 1158
TaqII GACCGA 1 cut(s) 225
TatI WGTACW 2 cut(s) 189, 1810
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 8 cut(s) 52, 337, 386, 391, 1160, 1343, 1570, 1583
Tru1I TTAA 6 cut(s) 9, 69, 488, 819, 1302, 1646
Tru9I TTAA 6 cut(s) 9, 69, 488, 819, 1302, 1646
TscAI CASTG 7 cut(s) 354, 444, 1065, 1329, 1366, 1410, 1548
TseFI GTSAC 4 cut(s) 181, 433, 650, 1483
TseI GCWGC 7 cut(s) 1212, 1261, 1331, 1522, 1638, 1729, 1801
Tsp45I GTSAC 4 cut(s) 181, 433, 650, 1483
TspDTI ATGAA 5 cut(s) 105, 212, 383, 869, 1666
TspGWI ACGGA 1 cut(s) 1776
TspRI CASTG 7 cut(s) 354, 444, 1065, 1329, 1366, 1410, 1548
VpaK11BI GGWCC 5 cut(s) 163, 208, 508, 608, 1652
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 4 cut(s) 379, 911, 1010, 1417
XbaI TCTAGA 1 cut(s) 907
XceI RCATGY 2 cut(s) 49, 1754
XhoI CTCGAG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 3 cut(s) 908, 1425, 1526
ZrmI AGTACT 1 cut(s) 1812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.