AT4G32900

Peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007778
Physical Location & Seq
Reverse (-)
15878569 .. 15881082
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32900.3

Sequence Viewer

Length: 423 bp
ATGTGGCCTTCGTCTCGAAACTCTTCGCAGCCTCAAGCTATGAGACAAGAAAAGAAGTCGATATCTGTGAGTTTTAGAGCAGAGAATCTCATACCAGGGGTTGTGATAGGATTCATCATAGGTATGATATTGGATTTATCACAGCAAGTGACCTCCCCAGTGAAAAGGAGCAGACTTTTATCCAGCAAGGTCCAGAAGCAGAGTTCTGTACCAGGCAATGGCAAGGACCAAGAGCTTAAAATGGTACTGGTAGTTAGGCAAGACTTGAAGATGAGAACGGGCAAAATTGCGTCACAGTGTGCACATGCTGCCACCGGCATGTATGCAGAGTTGATGCAGAGTGACCGATACCTTCTGCGGCGATGGGAGGAAAATGGGCAGCCAAAGATAGTCGTCACTTGCAAAAACCAGCAAGAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

140

Amino Acids

15.78

Weight (kDa)

10.07

Isoelectric Point (pI)

64.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 79 - 165 1e-31 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 218
AciI CCGC 1 cut(s) 358
AdeI CACNNNGTG 1 cut(s) 299
AfaI GTAC 2 cut(s) 210, 246
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 2 cut(s) 94, 211
AluBI AGCT 2 cut(s) 38, 235
AluI AGCT 2 cut(s) 38, 235
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 2 cut(s) 18, 37
Alw44I GTGCAC 1 cut(s) 300
AoxI GGCC 1 cut(s) 5
ApaLI GTGCAC 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 28, 308, 379
Asp700I GAANNNNTTC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 190, 226
AvaII GGWCC 2 cut(s) 190, 226
BaeGI GKGCMC 1 cut(s) 304
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 3 cut(s) 40, 295, 391
BccI CCATC 1 cut(s) 357
BciT130I CCWGG 2 cut(s) 96, 213
BcoDI GTCTC 2 cut(s) 18, 37
BisI GCNGC 4 cut(s) 29, 309, 359, 380
BlsI GCNGC 4 cut(s) 30, 310, 360, 381
Bme1390I CCNGG 2 cut(s) 96, 213
Bme18I GGWCC 2 cut(s) 190, 226
BmgT120I GGNCC 2 cut(s) 190, 226
BmrFI CCNGG 2 cut(s) 96, 213
BmrI ACTGGG 1 cut(s) 152
BmsI GCATC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 158, 252
Bse3DI GCAATG 1 cut(s) 223
BseBI CCWGG 2 cut(s) 96, 213
BseDI CCNNGG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 2 cut(s) 158, 252
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 3 cut(s) 40, 295, 391
BshFI GGCC 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 1 cut(s) 315
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 18, 37
BsmBI CGTCTC 1 cut(s) 18
BsnI GGCC 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 304
BspACI CCGC 1 cut(s) 358
BspANI GGCC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 2 cut(s) 158, 252
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 1 cut(s) 95
Bst2UI CCWGG 2 cut(s) 96, 213
Bst4CI ACNGT 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 308
BstMAI GTCTC 2 cut(s) 18, 37
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 2 cut(s) 96, 213
BstNSI RCATGY 2 cut(s) 308, 322
BstSCI CCNGG 2 cut(s) 94, 211
BstSLI GKGCMC 1 cut(s) 304
BstV1I GCAGC 3 cut(s) 40, 295, 391
BsuRI GGCC 1 cut(s) 7
BtgZI GCGATG 1 cut(s) 376
BtsIMutI CAGTG 2 cut(s) 165, 302
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 190, 226
CseI GACGC 1 cut(s) 279
Csp6I GTAC 2 cut(s) 209, 245
CviAII CATG 2 cut(s) 305, 319
CviJI RGCY 5 cut(s) 7, 31, 38, 235, 382
CviKI_1 RGCY 5 cut(s) 7, 31, 38, 235, 382
CviQI GTAC 2 cut(s) 209, 245
DraIII CACNNNGTG 1 cut(s) 299
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 63
Eco47I GGWCC 2 cut(s) 190, 226
EcoRII CCWGG 2 cut(s) 94, 211
EcoRV GATATC 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 2 cut(s) 308, 322
FaiI YATR 7 cut(s) 41, 92, 119, 125, 306, 320, 324
FatI CATG 2 cut(s) 304, 318
Fnu4HI GCNGC 4 cut(s) 29, 309, 359, 380
Fsp4HI GCNGC 4 cut(s) 29, 309, 359, 380
GluI GCNGC 4 cut(s) 29, 309, 359, 380
HaeIII GGCC 1 cut(s) 7
HapII CCGG 1 cut(s) 315
HgaI GACGC 1 cut(s) 279
Hin1II CATG 2 cut(s) 308, 322
HinfI GANTC 2 cut(s) 85, 111
HpaII CCGG 1 cut(s) 315
Hpy166II GTNNAC 1 cut(s) 302
Hpy188III TCNNGA 2 cut(s) 15, 193
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 18, 362
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4V TGCA 4 cut(s) 302, 326, 337, 402
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
Hsp92II CATG 2 cut(s) 308, 322
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 9 cut(s) 81, 108, 171, 196, 198, 206, 225, 233, 328
Lsp1109I GCAGC 3 cut(s) 40, 295, 391
LweI GCATC 1 cut(s) 324
MaeIII GTNAC 4 cut(s) 148, 291, 341, 394
MboII GAAGA 2 cut(s) 15, 280
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 1 cut(s) 285
MnlI CCTC 3 cut(s) 42, 163, 361
MroXI GAANNNNTTC 1 cut(s) 22
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 2 cut(s) 122, 317
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 2 cut(s) 96, 213
MvaI CCWGG 2 cut(s) 96, 213
MwoI GCNNNNNNNGC 1 cut(s) 308
NlaIII CATG 2 cut(s) 308, 322
NmuCI GTSAC 4 cut(s) 148, 291, 341, 394
NspI RCATGY 2 cut(s) 308, 322
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 2 cut(s) 85, 111
PflMI CCANNNNNTGG 1 cut(s) 218
PkrI GCNGC 4 cut(s) 30, 310, 360, 381
Psp6I CCWGG 2 cut(s) 94, 211
PspGI CCWGG 2 cut(s) 94, 211
PspPI GGNCC 2 cut(s) 190, 226
RsaI GTAC 2 cut(s) 210, 246
RsaNI GTAC 2 cut(s) 209, 245
RseI CAYNNNNRTG 2 cut(s) 122, 317
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 4 cut(s) 29, 309, 359, 380
Sau96I GGNCC 2 cut(s) 190, 226
ScrFI CCNGG 2 cut(s) 96, 213
SduI GDGCHC 1 cut(s) 304
SetI ASST 6 cut(s) 40, 124, 155, 192, 237, 354
SfaNI GCATC 1 cut(s) 324
SinI GGWCC 2 cut(s) 190, 226
SmiMI CAYNNNNRTG 2 cut(s) 122, 317
SmlI CTYRAG 1 cut(s) 33
SmoI CTYRAG 1 cut(s) 33
Sse9I AATT 1 cut(s) 285
SsiI CCGC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 94, 211
TaaI ACNGT 1 cut(s) 297
TaqI TCGA 2 cut(s) 16, 59
TaqII GACCGA 1 cut(s) 360
TasI AATT 1 cut(s) 285
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 2 cut(s) 85, 111
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 2 cut(s) 165, 302
TseFI GTSAC 4 cut(s) 148, 291, 341, 394
TseI GCWGC 3 cut(s) 28, 308, 379
Tsp45I GTSAC 4 cut(s) 148, 291, 341, 394
TspDTI ATGAA 1 cut(s) 103
TspRI CASTG 2 cut(s) 165, 302
Van91I CCANNNNNTGG 1 cut(s) 218
VneI GTGCAC 1 cut(s) 300
VpaK11BI GGWCC 2 cut(s) 190, 226
XceI RCATGY 2 cut(s) 308, 322
XmnI GAANNNNTTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.