Prupe.1G447600_v2.0.a1

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007778
Physical Location & Seq
Reverse (-)
37830833 .. 37833568
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G447600.2

Sequence Viewer

Length: 588 bp
ATGTTCGCTTCATTTCGAAACGCTTCCCAGCATCCAAACAAGCAGCAGCAGAAGCAAGAAAGAGAATGGTTATCTGTGAGTTTTAGGCCAGAGAACTTTATTCCGGGAGTTGTCATTGGCTTCATTCTTGGGTTGCTGTTGGATTTATCACAGCCCGGTAAGGGTCAGTCGAAGAAGAACAGTATCTCAATAGGCAAGGTGCAACAGCGAAGTTTGGTCACTAGTAATGGTGATGAAGAGCTTAAAATGGTTCTGGTAGTTCGACGAGACCTAAAGATGACAACTGGAAAAATTGCATCTCAGTGTGCTCATGCTGCCACTGGCGTGTATGCAGAACTGATGCAAAGCCACAGATCCCTTTTGAGACAATGGGAGCAATATGGGCAACCCAAAATAGTGGTTACATGCAAGAATCAACAAGAAATGAATAAGCTGAAGGAAGCAGCTGAGGGCATTGGCCTTCCTACTTTTGTTGTTGCTGATGCAGGGCGAACACAGGTTGTGAGCGGGTCTAAGACAGTTCTTGCCGTTGGACCTGGACCAAAAGAATCAGTTGATTCAGTGACAGGGAAACAAGCCCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

196

Amino Acids

21.32

Weight (kDa)

9.73

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 507
AciI CCGC 1 cut(s) 507
AclWI GGATC 1 cut(s) 348
AcuI CTGAAG 1 cut(s) 455
AfiI CCNNNNNNNGG 1 cut(s) 161
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 1 cut(s) 535
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 3 cut(s) 241, 433, 446
AluI AGCT 3 cut(s) 241, 433, 446
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 2 cut(s) 261, 358
AlwI GGATC 1 cut(s) 348
AoxI GGCC 2 cut(s) 86, 457
ApeKI GCWGC 4 cut(s) 43, 46, 314, 443
Asp700I GAANNNNTTC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 533, 539
AsuC2I CCSGG 2 cut(s) 105, 156
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 533, 539
Bbv12I GWGCWC 1 cut(s) 310
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 4 cut(s) 55, 58, 301, 455
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 1 cut(s) 537
BcnI CCSGG 2 cut(s) 105, 156
BcoDI GTCTC 2 cut(s) 261, 358
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 1 cut(s) 222
BisI GCNGC 4 cut(s) 44, 47, 315, 444
BlsI GCNGC 4 cut(s) 45, 48, 316, 445
Bme1390I CCNGG 3 cut(s) 105, 156, 537
Bme18I GGWCC 2 cut(s) 533, 539
BmgT120I GGNCC 2 cut(s) 533, 539
BmrFI CCNGG 3 cut(s) 105, 156, 537
BmsI GCATC 4 cut(s) 40, 305, 330, 472
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 1 cut(s) 16
BpuMI CCSGG 2 cut(s) 105, 156
BsaI GGTCTC 1 cut(s) 261
BsaXI ACNNNNNCTCC 1 cut(s) 564
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 289, 325
BseBI CCWGG 1 cut(s) 537
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 2 cut(s) 314, 438
BseNI ACTGG 2 cut(s) 289, 325
BseRI GAGGAG 1 cut(s) 572
BseXI GCAGC 4 cut(s) 55, 58, 301, 455
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 2 cut(s) 88, 459
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 2 cut(s) 104, 156
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 2 cut(s) 261, 358
BsnI GGCC 2 cut(s) 88, 459
Bso31I GGTCTC 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 1 cut(s) 353
BspACI CCGC 1 cut(s) 507
BspANI GGCC 2 cut(s) 88, 459
BspCNI CTCAG 2 cut(s) 313, 439
BspPI GGATC 1 cut(s) 348
BspQI GCTCTTC 1 cut(s) 231
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 507
BsrI ACTGG 2 cut(s) 289, 325
BssMI GATC 1 cut(s) 353
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 2 cut(s) 182, 520
Bst6I CTCTTC 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 300, 447, 513
BstF5I GGATG 1 cut(s) 31
BstKTI GATC 1 cut(s) 356
BstMAI GTCTC 2 cut(s) 261, 358
BstMBI GATC 1 cut(s) 353
BstMWI GCNNNNNNNGC 3 cut(s) 52, 314, 382
BstNI CCWGG 1 cut(s) 537
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 3 cut(s) 103, 154, 535
BstV1I GCAGC 4 cut(s) 55, 58, 301, 455
BstX2I RGATCY 1 cut(s) 353
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 1 cut(s) 353
BsuRI GGCC 2 cut(s) 88, 459
BtsCI GGATG 1 cut(s) 31
BtsIMutI CAGTG 3 cut(s) 308, 318, 567
Cfr13I GGNCC 2 cut(s) 533, 539
CviAII CATG 2 cut(s) 311, 405
CviJI RGCY 9 cut(s) 88, 120, 154, 241, 348, 433, 446, 459, 578
CviKI_1 RGCY 9 cut(s) 88, 120, 154, 241, 348, 433, 446, 459, 578
DdeI CTNAG 3 cut(s) 300, 447, 513
DpnI GATC 1 cut(s) 355
DpnII GATC 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 533, 539
Eco57I CTGAAG 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 535
FaeI CATG 2 cut(s) 314, 408
FaiI YATR 4 cut(s) 312, 330, 381, 406
FatI CATG 2 cut(s) 310, 404
FauI CCCGC 1 cut(s) 500
Fnu4HI GCNGC 4 cut(s) 44, 47, 315, 444
FokI GGATG 1 cut(s) 18
Fsp4HI GCNGC 4 cut(s) 44, 47, 315, 444
FspBI CTAG 1 cut(s) 222
GluI GCNGC 4 cut(s) 44, 47, 315, 444
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 2 cut(s) 88, 459
HapII CCGG 2 cut(s) 104, 156
Hin1II CATG 2 cut(s) 314, 408
HinfI GANTC 3 cut(s) 412, 548, 557
HpaII CCGG 2 cut(s) 104, 156
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 1 cut(s) 587
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 430, 470
HpyCH4III ACNGT 2 cut(s) 182, 520
HpyCH4V TGCA 6 cut(s) 202, 296, 332, 343, 408, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 314, 382
HpyF3I CTNAG 3 cut(s) 300, 447, 513
Hsp92II CATG 2 cut(s) 314, 408
Kzo9I GATC 1 cut(s) 353
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 4 cut(s) 55, 58, 301, 455
LweI GCATC 4 cut(s) 40, 305, 330, 472
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 3 cut(s) 217, 400, 562
MalI GATC 1 cut(s) 355
MbiI CCGCTC 1 cut(s) 507
MboI GATC 1 cut(s) 353
MboII GAAGA 3 cut(s) 184, 187, 248
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 1 cut(s) 291
MmeI TCCRAC 2 cut(s) 120, 511
MnlI CCTC 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 22
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 2 cut(s) 301, 323
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 2 cut(s) 104, 156
MspR9I CCNGG 3 cut(s) 105, 156, 537
MvaI CCWGG 1 cut(s) 537
MwoI GCNNNNNNNGC 3 cut(s) 52, 314, 382
NciI CCSGG 2 cut(s) 105, 156
NdeII GATC 1 cut(s) 353
NlaIII CATG 2 cut(s) 314, 408
NmuCI GTSAC 2 cut(s) 217, 562
NspI RCATGY 1 cut(s) 408
NspV TTCGAA 1 cut(s) 16
OliI CACNNNNGTG 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 22
PfeI GAWTC 3 cut(s) 412, 548, 557
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 4 cut(s) 45, 48, 316, 445
Psp6I CCWGG 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 535
PspPI GGNCC 2 cut(s) 533, 539
PsuI RGATCY 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 446
RseI CAYNNNNRTG 2 cut(s) 301, 323
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 44, 47, 315, 444
Sau3AI GATC 1 cut(s) 353
Sau96I GGNCC 2 cut(s) 533, 539
ScrFI CCNGG 3 cut(s) 105, 156, 537
SduI GDGCHC 1 cut(s) 310
SetI ASST 7 cut(s) 201, 243, 273, 435, 448, 501, 538
SfaNI GCATC 4 cut(s) 40, 305, 330, 472
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 533, 539
SmiMI CAYNNNNRTG 2 cut(s) 301, 323
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 1 cut(s) 291
SsiI CCGC 1 cut(s) 507
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 103, 154, 535
TaaI ACNGT 2 cut(s) 182, 520
TaqI TCGA 3 cut(s) 16, 170, 262
TasI AATT 1 cut(s) 291
TfiI GAWTC 3 cut(s) 412, 548, 557
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 3 cut(s) 308, 325, 567
TseFI GTSAC 2 cut(s) 217, 562
TseI GCWGC 4 cut(s) 43, 46, 314, 443
Tsp45I GTSAC 2 cut(s) 217, 562
TspDTI ATGAA 3 cut(s) 112, 249, 440
TspRI CASTG 3 cut(s) 308, 325, 567
VpaK11BI GGWCC 2 cut(s) 533, 539
XceI RCATGY 1 cut(s) 408
XmnI GAANNNNTTC 1 cut(s) 22
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.