FvH4_2g35470

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007778
Physical Location & Seq
Reverse (-)
26142978 .. 26145952
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35470.t2

Sequence Viewer

Length: 591 bp
ATGTTTGGTTCGTTAAGGATAGCTTCCCAGAATCAAAGAAAGCAGCCGAAGCAAGAAAGAGAATGGTTGAACTTGAGTTTTAAGCCAGAGAATTTCCTTCCAGGAGTTGTCATTGGCTTCATTCTTGGCTTGTTGTTGGATTTATCGCAACCTGGTAAGGGTTCATTAAGGTTAAAGAGGAAAAATTTCTCAGAGGGTAAGCCCCAGCAGCGGAGTTTGGTCTCGAGTAATGGTGATGAAGAGCTTAAAATGGTTCTTGTAGTTAGACGAGACTTGAAGATGACAACCGGAAAAATTGCATCTCAGTGTGCTCATGCCGCCACTGGCATGTATGCAGAACTGATGCAAAGTCATAGGTCACTTTTGAGACAATGGGAGCAAAATGGGCAGCCCAAAATAGTGGTTACATGCAAGAGTCAACAAGAAATGAATAAGCTGAAAGAAGCAGCTGATGGCATTGGCCTTCCAACATTTGTTGTCGCAGATGCAGGGCGAACACAGGTTGCCAGTGGCTCAAAGACAGTTCTTGCCGTTGGACCTGGACCAAAAGAATTGGTGGATTCAGTGACAGGGAAACAGGGCCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

197

Amino Acids

21.46

Weight (kDa)

9.92

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 83 - 194 2.3e-44 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 211, 318
AcsI RAATTY 2 cut(s) 91, 184
AfiI CCNNNNNNNGG 2 cut(s) 158, 210
AgsI TTSAA 2 cut(s) 70, 277
AjnI CCWGG 3 cut(s) 100, 151, 538
AluBI AGCT 4 cut(s) 23, 244, 436, 449
AluI AGCT 4 cut(s) 23, 244, 436, 449
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 3 cut(s) 226, 264, 361
Ama87I CYCGRG 1 cut(s) 223
AoxI GGCC 2 cut(s) 460, 580
ApeKI GCWGC 4 cut(s) 43, 208, 388, 446
ApoI RAATTY 2 cut(s) 91, 184
Asp700I GAANNNNTTC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 536, 542, 580
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 2 cut(s) 536, 542
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 55, 220, 400, 458
BccI CCATC 1 cut(s) 446
BceAI ACGGC 1 cut(s) 515
BciT130I CCWGG 3 cut(s) 102, 153, 540
BcoDI GTCTC 3 cut(s) 226, 264, 361
BisI GCNGC 5 cut(s) 44, 209, 318, 389, 447
BlsI GCNGC 5 cut(s) 45, 210, 319, 390, 448
Bme1390I CCNGG 3 cut(s) 102, 153, 540
Bme18I GGWCC 2 cut(s) 536, 542
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 536, 542, 580
BmrFI CCNGG 3 cut(s) 102, 153, 540
BmsI GCATC 3 cut(s) 308, 333, 475
BpuEI CTTGAG 1 cut(s) 94
BsaI GGTCTC 1 cut(s) 226
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 1 cut(s) 567
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 210
Bse1I ACTGG 2 cut(s) 328, 507
BseBI CCWGG 3 cut(s) 102, 153, 540
BseLI CCNNNNNNNGG 2 cut(s) 158, 210
BseMII CTCAG 2 cut(s) 204, 317
BseNI ACTGG 2 cut(s) 328, 507
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 4 cut(s) 55, 220, 400, 458
BseYI CCCAGC 1 cut(s) 204
BshFI GGCC 2 cut(s) 462, 582
BsiHKAI GWGCWC 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 158, 210
BsmAI GTCTC 3 cut(s) 226, 264, 361
BsnI GGCC 2 cut(s) 462, 582
Bso31I GGTCTC 1 cut(s) 226
BsoBI CYCGRG 1 cut(s) 223
Bsp1286I GDGCHC 1 cut(s) 313
BspACI CCGC 2 cut(s) 211, 318
BspANI GGCC 2 cut(s) 462, 582
BspCNI CTCAG 2 cut(s) 203, 316
BspQI GCTCTTC 1 cut(s) 234
BspTNI GGTCTC 1 cut(s) 226
BsrI ACTGG 2 cut(s) 328, 507
Bst2UI CCWGG 3 cut(s) 102, 153, 540
Bst4CI ACNGT 1 cut(s) 523
Bst6I CTCTTC 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 190, 303
BstMAI GTCTC 3 cut(s) 226, 264, 361
BstMWI GCNNNNNNNGC 4 cut(s) 49, 208, 317, 385
BstNI CCWGG 3 cut(s) 102, 153, 540
BstNSI RCATGY 2 cut(s) 331, 411
BstSCI CCNGG 3 cut(s) 100, 151, 538
BstV1I GCAGC 4 cut(s) 55, 220, 400, 458
BstXI CCANNNNNNTGG 1 cut(s) 400
BsuRI GGCC 2 cut(s) 462, 582
BtsIMutI CAGTG 4 cut(s) 311, 321, 514, 570
Cfr13I GGNCC 3 cut(s) 536, 542, 580
CsiI ACCWGGT 1 cut(s) 151
CviAII CATG 3 cut(s) 314, 328, 408
DdeI CTNAG 2 cut(s) 190, 303
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 226
Eco47I GGWCC 2 cut(s) 536, 542
Eco88I CYCGRG 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 580
EcoRII CCWGG 3 cut(s) 100, 151, 538
FaeI CATG 3 cut(s) 317, 331, 411
FaiI YATR 5 cut(s) 315, 329, 333, 354, 409
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FatI CATG 3 cut(s) 313, 327, 407
Fnu4HI GCNGC 5 cut(s) 44, 209, 318, 389, 447
Fsp4HI GCNGC 5 cut(s) 44, 209, 318, 389, 447
GluI GCNGC 5 cut(s) 44, 209, 318, 389, 447
GsaI CCCAGC 1 cut(s) 208
HaeIII GGCC 2 cut(s) 462, 582
HapII CCGG 1 cut(s) 288
Hin1II CATG 3 cut(s) 317, 331, 411
HincII GTYRAC 1 cut(s) 419
HindII GTYRAC 1 cut(s) 419
HinfI GANTC 3 cut(s) 31, 415, 560
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 2 cut(s) 193, 590
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 419
HpyAV CCTTC 2 cut(s) 107, 473
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4V TGCA 5 cut(s) 299, 335, 346, 411, 488
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 208, 317, 385
HpyF3I CTNAG 2 cut(s) 190, 303
Hsp92II CATG 3 cut(s) 317, 331, 411
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 4 cut(s) 55, 220, 400, 458
LweI GCATC 3 cut(s) 308, 333, 475
MabI ACCWGGT 1 cut(s) 151
MaeIII GTNAC 3 cut(s) 357, 403, 565
MboII GAAGA 2 cut(s) 251, 289
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 4 cut(s) 91, 184, 294, 551
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 3 cut(s) 117, 491, 514
MnlI CCTC 2 cut(s) 171, 187
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 5 cut(s) 14, 81, 167, 173, 246
MslI CAYNNNNRTG 2 cut(s) 304, 326
MspA1I CMGCKG 2 cut(s) 211, 449
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 3 cut(s) 102, 153, 540
MvaI CCWGG 3 cut(s) 102, 153, 540
MwoI GCNNNNNNNGC 4 cut(s) 49, 208, 317, 385
NlaIII CATG 3 cut(s) 317, 331, 411
NmuCI GTSAC 2 cut(s) 357, 565
NspI RCATGY 2 cut(s) 331, 411
PaeR7I CTCGAG 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 2 cut(s) 31, 560
PfoI TCCNGGA 1 cut(s) 100
PkrI GCNGC 5 cut(s) 45, 210, 319, 390, 448
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
Psp6I CCWGG 3 cut(s) 100, 151, 538
PspFI CCCAGC 1 cut(s) 204
PspGI CCWGG 3 cut(s) 100, 151, 538
PspPI GGNCC 3 cut(s) 536, 542, 580
PvuII CAGCTG 1 cut(s) 449
RseI CAYNNNNRTG 2 cut(s) 304, 326
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 5 cut(s) 14, 81, 167, 173, 246
SatI GCNGC 5 cut(s) 44, 209, 318, 389, 447
Sau96I GGNCC 3 cut(s) 536, 542, 580
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 3 cut(s) 102, 153, 540
SduI GDGCHC 1 cut(s) 313
SetI ASST 9 cut(s) 25, 154, 173, 246, 359, 438, 451, 504, 541
SexAI ACCWGGT 1 cut(s) 151
SfaNI GCATC 3 cut(s) 308, 333, 475
Sfr274I CTCGAG 1 cut(s) 223
SinI GGWCC 2 cut(s) 536, 542
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 304, 326
SmlI CTYRAG 2 cut(s) 73, 223
SmoI CTYRAG 2 cut(s) 73, 223
Sse9I AATT 4 cut(s) 91, 184, 294, 551
SsiI CCGC 2 cut(s) 211, 318
StyD4I CCNGG 3 cut(s) 100, 151, 538
TaaI ACNGT 1 cut(s) 523
TaqI TCGA 1 cut(s) 224
TasI AATT 4 cut(s) 91, 184, 294, 551
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 2 cut(s) 31, 560
Tru1I TTAA 5 cut(s) 14, 81, 167, 173, 246
Tru9I TTAA 5 cut(s) 14, 81, 167, 173, 246
TscAI CASTG 4 cut(s) 311, 328, 514, 570
TseFI GTSAC 2 cut(s) 357, 565
TseI GCWGC 4 cut(s) 43, 208, 388, 446
Tsp45I GTSAC 2 cut(s) 357, 565
TspDTI ATGAA 4 cut(s) 109, 153, 252, 443
TspRI CASTG 4 cut(s) 311, 328, 514, 570
VpaK11BI GGWCC 2 cut(s) 536, 542
XapI RAATTY 2 cut(s) 91, 184
XceI RCATGY 2 cut(s) 331, 411
XhoI CTCGAG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.