MD15G1092700.v1.1

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007778
Physical Location & Seq
Reverse (-)
6443112 .. 6446175
3064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1092700.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTTTCGAAACGCTTCTCAGCATCCAAACAAGCCACAGAAGCAAGAAAGAGAATGGTTGGCAGTGAGCTTTAAGCCAGAGAACTTCATTCCAGGAGTTGTCATTGGCTTCATCCTTGGGTTGTTGCTGGATTTATCACAGCCTGGTAAGGGTCGATCAAAGAAGAACACTGCTTCGGTAGGCCAGCTCCAACAGCGGAGTTTGGTCACAAGCAATGTCAATGGTGGTGAAGAACTTAAAATGGTACTGGTAGTTAGACGAGACCTAAAGATGACAACGGGTAAAATTGCATCTCAGTGTGCTCATGCTGCCACTGGCGCGTATGCAGAACTGATGCAAAGCCACAGATCCCTTTTGAGACAGTGGGAGCAATACGGGCAACCCAAAATAGTGGTTACATGCAAGAATCAACAAGAAATGAATAAGCTGAAGGAAGCAGCTGAGAACATTGGCATTCCAACTTTTGTCGTTGCTGATGCAGGGCGAACACAGGTTGTGAGTGGATCGAAGACAGTTCTTGCCATTGGACCTGGACCAAAAGAATCAGTAGATTCAGTGACTGGGAAACAGGCTCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

194

Amino Acids

21.01

Weight (kDa)

9.82

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 79 - 192 1.2e-44 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 320
AciI CCGC 1 cut(s) 196
AclWI GGATC 2 cut(s) 342, 511
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 149
AjnI CCWGG 3 cut(s) 91, 142, 529
AluBI AGCT 4 cut(s) 69, 187, 427, 440
AluI AGCT 4 cut(s) 69, 187, 427, 440
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 2 cut(s) 255, 352
AlwI GGATC 2 cut(s) 342, 511
AlwNI CAGNNNCTG 1 cut(s) 560
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 2 cut(s) 308, 437
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 527, 533
AsuHPI GGTGA 1 cut(s) 239
AsuII TTCGAA 1 cut(s) 7
AvaII GGWCC 2 cut(s) 527, 533
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 295, 449
BciT130I CCWGG 3 cut(s) 93, 144, 531
BcoDI GTCTC 2 cut(s) 255, 352
BisI GCNGC 2 cut(s) 309, 438
BlsI GCNGC 2 cut(s) 310, 439
Bme1390I CCNGG 3 cut(s) 93, 144, 531
Bme18I GGWCC 2 cut(s) 527, 533
BmgT120I GGNCC 2 cut(s) 527, 533
BmrFI CCNGG 3 cut(s) 93, 144, 531
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 4 cut(s) 31, 299, 324, 466
BmuI ACTGGG 1 cut(s) 570
BpiI GAAGAC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 7
BsaI GGTCTC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 115
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 252, 319, 565
Bse3DI GCAATG 1 cut(s) 220
BseBI CCWGG 3 cut(s) 93, 144, 531
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 2 cut(s) 22, 111
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 3 cut(s) 32, 308, 432
BseNI ACTGG 3 cut(s) 252, 319, 565
BseXI GCAGC 2 cut(s) 295, 449
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 304
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 2 cut(s) 255, 352
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 1 cut(s) 183
Bso31I GGTCTC 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 3 cut(s) 155, 347, 503
BspACI CCGC 1 cut(s) 196
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 3 cut(s) 31, 307, 433
BspFNI CGCG 1 cut(s) 320
BspPI GGATC 2 cut(s) 342, 511
BspQI GCTCTTC 1 cut(s) 579
BspT104I TTCGAA 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 220
BsrI ACTGG 3 cut(s) 252, 319, 565
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 3 cut(s) 155, 347, 503
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 3 cut(s) 93, 144, 531
Bst4CI ACNGT 2 cut(s) 363, 514
Bst6I CTCTTC 1 cut(s) 579
BstBI TTCGAA 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 3 cut(s) 18, 294, 441
BstF5I GGATG 2 cut(s) 22, 111
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 3 cut(s) 158, 350, 506
BstMAI GTCTC 2 cut(s) 255, 352
BstMBI GATC 3 cut(s) 155, 347, 503
BstMWI GCNNNNNNNGC 5 cut(s) 40, 193, 308, 317, 376
BstNI CCWGG 3 cut(s) 93, 144, 531
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 3 cut(s) 91, 142, 529
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 295, 449
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 1 cut(s) 347
BsuRI GGCC 1 cut(s) 183
BtsCI GGATG 2 cut(s) 22, 111
BtsI GCAGTG 2 cut(s) 69, 168
BtsIMutI CAGTG 6 cut(s) 69, 168, 302, 312, 368, 561
Cac8I GCNNGC 1 cut(s) 185
CaiI CAGNNNCTG 1 cut(s) 560
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 527, 533
Csp6I GTAC 1 cut(s) 245
CviAII CATG 2 cut(s) 305, 399
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 3 cut(s) 18, 294, 441
DpnI GATC 3 cut(s) 157, 349, 505
DpnII GATC 3 cut(s) 155, 347, 503
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 527, 533
Eco57I CTGAAG 1 cut(s) 449
EcoRII CCWGG 3 cut(s) 91, 142, 529
EcoT14I CCWWGG 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 2 cut(s) 308, 402
FaiI YATR 4 cut(s) 306, 324, 400, 580
FatI CATG 2 cut(s) 304, 398
Fnu4HI GCNGC 2 cut(s) 309, 438
FokI GGATG 2 cut(s) 9, 98
Fsp4HI GCNGC 2 cut(s) 309, 438
GlaI GCGC 1 cut(s) 319
GluI GCNGC 2 cut(s) 309, 438
HaeIII GGCC 1 cut(s) 183
HhaI GCGC 1 cut(s) 320
Hin1II CATG 2 cut(s) 308, 402
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HinfI GANTC 3 cut(s) 406, 542, 551
HphI GGTGA 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 2 cut(s) 363, 514
HpyCH4V TGCA 5 cut(s) 290, 326, 337, 402, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 193, 308, 317, 376
HpyF3I CTNAG 3 cut(s) 18, 294, 441
Hsp92II CATG 2 cut(s) 308, 402
HspAI GCGC 1 cut(s) 318
Kzo9I GATC 3 cut(s) 155, 347, 503
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 2 cut(s) 192, 367
Lsp1109I GCAGC 2 cut(s) 295, 449
LweI GCATC 4 cut(s) 31, 299, 324, 466
MaeIII GTNAC 3 cut(s) 205, 394, 556
MalI GATC 3 cut(s) 157, 349, 505
MboI GATC 3 cut(s) 155, 347, 503
MboII GAAGA 4 cut(s) 175, 242, 520, 566
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 1 cut(s) 285
MmeI TCCRAC 2 cut(s) 214, 482
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 2 cut(s) 72, 237
MslI CAYNNNNRTG 1 cut(s) 295
MspA1I CMGCKG 2 cut(s) 196, 440
MspR9I CCNGG 3 cut(s) 93, 144, 531
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 3 cut(s) 93, 144, 531
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 5 cut(s) 40, 193, 308, 317, 376
NdeII GATC 3 cut(s) 155, 347, 503
NlaIII CATG 2 cut(s) 308, 402
NmuCI GTSAC 2 cut(s) 205, 556
NspI RCATGY 1 cut(s) 402
NspV TTCGAA 1 cut(s) 7
PciSI GCTCTTC 1 cut(s) 579
PctI GAATGC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 3 cut(s) 406, 542, 551
PfoI TCCNGGA 1 cut(s) 91
PkrI GCNGC 2 cut(s) 310, 439
Psp6I CCWGG 3 cut(s) 91, 142, 529
PspGI CCWGG 3 cut(s) 91, 142, 529
PspPI GGNCC 2 cut(s) 527, 533
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 1 cut(s) 347
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
RseI CAYNNNNRTG 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 2 cut(s) 72, 237
SatI GCNGC 2 cut(s) 309, 438
Sau3AI GATC 3 cut(s) 155, 347, 503
Sau96I GGNCC 2 cut(s) 527, 533
ScrFI CCNGG 3 cut(s) 93, 144, 531
SduI GDGCHC 1 cut(s) 304
SetI ASST 7 cut(s) 71, 189, 267, 429, 442, 495, 532
SfaNI GCATC 4 cut(s) 31, 299, 324, 466
SfuI TTCGAA 1 cut(s) 7
SinI GGWCC 2 cut(s) 527, 533
SmiMI CAYNNNNRTG 1 cut(s) 295
Sse9I AATT 1 cut(s) 285
SsiI CCGC 1 cut(s) 196
StyD4I CCNGG 3 cut(s) 91, 142, 529
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 363, 514
TaqI TCGA 3 cut(s) 7, 154, 506
TasI AATT 1 cut(s) 285
TfiI GAWTC 3 cut(s) 406, 542, 551
Tru1I TTAA 2 cut(s) 72, 237
Tru9I TTAA 2 cut(s) 72, 237
TscAI CASTG 6 cut(s) 69, 175, 302, 319, 368, 561
TseFI GTSAC 2 cut(s) 205, 556
TseI GCWGC 2 cut(s) 308, 437
Tsp45I GTSAC 2 cut(s) 205, 556
TspDTI ATGAA 3 cut(s) 76, 100, 434
TspRI CASTG 6 cut(s) 69, 175, 302, 319, 368, 561
VpaK11BI GGWCC 2 cut(s) 527, 533
XceI RCATGY 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.