Rh6AG461400

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0007778
Physical Location & Seq
Forward (+)
64485727 .. 64490611
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG461400.1

Sequence Viewer

Length: 558 bp
ATGTTTGGTTCATTAAGAATTTCTTCCCAGAATCCAAGAAAGCAGCAGAAGCAAGAACGAGAATGGTTAAACTTGAGTTTTAAGCCAGAGAATTTCCTTCCAGGAGTTGTCATTGGCTTCATTCTTGGGTTGTTGTTGGATTTATCACAACCTGGTAAGGGTTCCTTGAAGTTGAAGAGGAAAAATTTCTCAGAGGGTAAGCCCCAACAGCGAAGTTTGGTCTCGACTAATGGTGATGAAGAGCTTAAAATGGTTCTTGTAGTTAGACGAGACTTAAAGATTACAACTGGAAAAATCGCATCTCAGTGTGCTCATGCTGCCACCGGCATGTATGCAGAACTGATGCAAAGCCGTAGGTCCCTTTTGAGACAATGGGAGGAAAATGGGCAACCCAAAATTGTGGTTACATGCAAGAATCAACAAGAAATGAATAAGCTGAAAGAAGCAGCTGAGGGCATTGGCCTTCCAACTTTTGTTGTCGCTGATGCAGGGCGAACACAGGTAAGATGTAATCTTGGAATGTACAGTATTAGTCTGAAGTTGGTTGAATCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

185

Amino Acids

20.72

Weight (kDa)

9.83

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 82 - 170 7.5e-32 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 18, 91, 184
AcuI CTGAAG 1 cut(s) 557
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 3 cut(s) 169, 175, 548
AjnI CCWGG 2 cut(s) 100, 151
AluBI AGCT 3 cut(s) 244, 436, 449
AluI AGCT 3 cut(s) 244, 436, 449
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 3 cut(s) 226, 264, 361
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 3 cut(s) 43, 317, 446
ApoI RAATTY 3 cut(s) 18, 91, 184
Asp700I GAANNNNTTC 2 cut(s) 22, 185
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 245
AvaII GGWCC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 313
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 55, 304, 458
BceAI ACGGC 1 cut(s) 336
BciT130I CCWGG 2 cut(s) 102, 153
BcoDI GTCTC 3 cut(s) 226, 264, 361
BisI GCNGC 3 cut(s) 44, 318, 447
BlsI GCNGC 3 cut(s) 45, 319, 448
Bme1390I CCNGG 2 cut(s) 102, 153
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 2 cut(s) 163, 359
BmrFI CCNGG 2 cut(s) 102, 153
BmsI GCATC 3 cut(s) 308, 333, 475
Bpu10I CCTNAGC 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 94
BsaI GGTCTC 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 1 cut(s) 292
BseBI CCWGG 2 cut(s) 102, 153
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 3 cut(s) 204, 317, 441
BseNI ACTGG 1 cut(s) 292
BseXI GCAGC 3 cut(s) 55, 304, 458
BshFI GGCC 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 3 cut(s) 226, 264, 361
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 1 cut(s) 462
Bso31I GGTCTC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 313
Bsp1407I TGTACA 1 cut(s) 522
BspANI GGCC 1 cut(s) 462
BspCNI CTCAG 3 cut(s) 203, 316, 442
BspLI GGNNCC 2 cut(s) 163, 359
BspQI GCTCTTC 1 cut(s) 234
BspTNI GGTCTC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 323
Bst2UI CCWGG 2 cut(s) 102, 153
Bst4CI ACNGT 1 cut(s) 527
Bst6I CTCTTC 2 cut(s) 170, 234
BstAUI TGTACA 1 cut(s) 522
BstDEI CTNAG 3 cut(s) 190, 303, 450
BstMAI GTCTC 3 cut(s) 226, 264, 361
BstMWI GCNNNNNNNGC 3 cut(s) 49, 208, 317
BstNI CCWGG 2 cut(s) 102, 153
BstNSI RCATGY 2 cut(s) 331, 411
BstSCI CCNGG 2 cut(s) 100, 151
BstV1I GCAGC 3 cut(s) 55, 304, 458
BstXI CCANNNNNNTGG 1 cut(s) 400
BsuRI GGCC 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 357
CsiI ACCWGGT 1 cut(s) 151
Csp6I GTAC 1 cut(s) 523
CviAII CATG 3 cut(s) 314, 328, 408
CviJI RGCY 8 cut(s) 85, 117, 202, 244, 351, 436, 449, 462
CviKI_1 RGCY 8 cut(s) 85, 117, 202, 244, 351, 436, 449, 462
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 3 cut(s) 190, 303, 450
Eam1104I CTCTTC 2 cut(s) 170, 234
EarI CTCTTC 2 cut(s) 170, 234
Eco31I GGTCTC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 100, 151
FaeI CATG 3 cut(s) 317, 331, 411
FaiI YATR 6 cut(s) 315, 329, 333, 409, 554, 556
FalI AAGNNNNNCTT 4 cut(s) 7, 39, 149, 181
FaqI GGGAC 1 cut(s) 343
FatI CATG 3 cut(s) 313, 327, 407
Fnu4HI GCNGC 3 cut(s) 44, 318, 447
Fsp4HI GCNGC 3 cut(s) 44, 318, 447
GluI GCNGC 3 cut(s) 44, 318, 447
HaeIII GGCC 1 cut(s) 462
HapII CCGG 1 cut(s) 324
Hin1II CATG 3 cut(s) 317, 331, 411
HinfI GANTC 3 cut(s) 31, 415, 548
HpaII CCGG 1 cut(s) 324
HphI GGTGA 1 cut(s) 245
Hpy188I TCNGA 2 cut(s) 193, 537
Hpy188III TCNNGA 1 cut(s) 223
HpyAV CCTTC 2 cut(s) 107, 473
HpyCH4III ACNGT 1 cut(s) 527
HpyCH4V TGCA 4 cut(s) 335, 346, 411, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 208, 317
HpyF3I CTNAG 3 cut(s) 190, 303, 450
Hsp92II CATG 3 cut(s) 317, 331, 411
LguI GCTCTTC 1 cut(s) 234
Lsp1109I GCAGC 3 cut(s) 55, 304, 458
LweI GCATC 3 cut(s) 308, 333, 475
MabI ACCWGGT 1 cut(s) 151
MaeIII GTNAC 1 cut(s) 403
MboII GAAGA 3 cut(s) 15, 187, 251
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 4 cut(s) 18, 91, 184, 396
MmeI TCCRAC 2 cut(s) 117, 491
MnlI CCTC 4 cut(s) 171, 187, 370, 445
MroXI GAANNNNTTC 2 cut(s) 22, 185
MseI TTAA 5 cut(s) 14, 68, 81, 246, 275
MslI CAYNNNNRTG 2 cut(s) 304, 326
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 2 cut(s) 102, 153
MvaI CCWGG 2 cut(s) 102, 153
MwoI GCNNNNNNNGC 3 cut(s) 49, 208, 317
NlaIII CATG 3 cut(s) 317, 331, 411
NlaIV GGNNCC 2 cut(s) 163, 359
NspI RCATGY 2 cut(s) 331, 411
PciSI GCTCTTC 1 cut(s) 234
PdmI GAANNNNTTC 2 cut(s) 22, 185
PfeI GAWTC 3 cut(s) 31, 415, 548
PfoI TCCNGGA 1 cut(s) 100
PkrI GCNGC 3 cut(s) 45, 319, 448
PpuMI RGGWCCY 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 100, 151
PspGI CCWGG 2 cut(s) 100, 151
PspN4I GGNNCC 2 cut(s) 163, 359
PspPI GGNCC 1 cut(s) 357
PspPPI RGGWCCY 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
RseI CAYNNNNRTG 2 cut(s) 304, 326
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 5 cut(s) 14, 68, 81, 246, 275
SatI GCNGC 3 cut(s) 44, 318, 447
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 2 cut(s) 102, 153
SduI GDGCHC 1 cut(s) 313
SetI ASST 6 cut(s) 154, 246, 359, 438, 451, 504
SexAI ACCWGGT 1 cut(s) 151
SfaNI GCATC 3 cut(s) 308, 333, 475
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 2 cut(s) 304, 326
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 4 cut(s) 18, 91, 184, 396
StyD4I CCNGG 2 cut(s) 100, 151
TaaI ACNGT 1 cut(s) 527
TaqI TCGA 1 cut(s) 224
TasI AATT 4 cut(s) 18, 91, 184, 396
TatI WGTACW 1 cut(s) 522
TfiI GAWTC 3 cut(s) 31, 415, 548
Tru1I TTAA 5 cut(s) 14, 68, 81, 246, 275
Tru9I TTAA 5 cut(s) 14, 68, 81, 246, 275
TscAI CASTG 1 cut(s) 311
TseI GCWGC 3 cut(s) 43, 317, 446
TspDTI ATGAA 3 cut(s) 109, 252, 443
TspRI CASTG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 3 cut(s) 18, 91, 184
XceI RCATGY 2 cut(s) 331, 411
XmnI GAANNNNTTC 2 cut(s) 22, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.