Rw6G040210

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007778
Physical Location & Seq
Forward (+)
64073980 .. 64077249
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040210.1

Sequence Viewer

Length: 594 bp
ATGTTTGGTTCATCAAGAATTTCTTCCCAGAATCCAAGAAAGCAGCAGCAGAAGCAAGAACGAGAATGGTTAAACTTGAGTTTTAAGCCTGAGAATTTCCTTCCAGGAGTTGTCATTGGCTTCATTCTTGGGTTGTTGTTGGATTTATCACAACCTGGTAAGGGTTCCTTGAAGTTGAAGAGGAAAAATTTCTCAGAGGGTAAGCCCCAACAGCGAAGTTTGGTCTCGACTAATGGTGATGAAGAGCTTAAAATGGTTCTTGTAGTTAGACGAGACTTAAAGATGACAACTGGAAAAATCGCATCTCAGTGTGCTCATGCTGCCACTGGCATGTATGCAGAACTGATGCAAAGCCATAGGTCCCTTTTGAGACAATGGGAGGAAAATGGGCAACCCAAAATAGTGGTTACTTGCAAGAATCAACAAGAAATGAATAAGCTGAAAGAAGCAGCTGAGGGCATTGGCCTTCCAACTTTTGTTGTCGCTGATGCAGGGCGAACACAGGTTGTGAGTGGGTCAAAGACAGTTCTTGCCGTTGGACCAGGACCAAAAGAAGTGGTAGATTCAGTGACAGGGAAACAGGGTCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

197

Amino Acids

21.62

Weight (kDa)

9.79

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 83 - 194 5.8e-44 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 18, 94, 187
AfiI CCNNNNNNNGG 1 cut(s) 161
AgsI TTSAA 2 cut(s) 172, 178
AjnI CCWGG 3 cut(s) 103, 154, 541
AluBI AGCT 3 cut(s) 247, 439, 452
AluI AGCT 3 cut(s) 247, 439, 452
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 4 cut(s) 229, 267, 364, 590
AoxI GGCC 1 cut(s) 463
ApeKI GCWGC 4 cut(s) 43, 46, 320, 449
ApoI RAATTY 3 cut(s) 18, 94, 187
Asp700I GAANNNNTTC 2 cut(s) 22, 188
AspS9I GGNCC 3 cut(s) 360, 539, 545
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 3 cut(s) 360, 539, 545
Bbv12I GWGCWC 1 cut(s) 316
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 4 cut(s) 55, 58, 307, 461
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 3 cut(s) 105, 156, 543
BcoDI GTCTC 4 cut(s) 229, 267, 364, 590
BisI GCNGC 4 cut(s) 44, 47, 321, 450
BlsI GCNGC 4 cut(s) 45, 48, 322, 451
Bme1390I CCNGG 3 cut(s) 105, 156, 543
Bme18I GGWCC 3 cut(s) 360, 539, 545
BmgT120I GGNCC 3 cut(s) 360, 539, 545
BmiI GGNNCC 2 cut(s) 166, 362
BmrFI CCNGG 3 cut(s) 105, 156, 543
BmsI GCATC 3 cut(s) 311, 336, 478
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 97
BsaI GGTCTC 2 cut(s) 229, 590
BsaXI ACNNNNNCTCC 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 295, 331
BseBI CCWGG 3 cut(s) 105, 156, 543
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMII CTCAG 4 cut(s) 81, 207, 320, 444
BseNI ACTGG 2 cut(s) 295, 331
BseRI GAGGAG 1 cut(s) 578
BseXI GCAGC 4 cut(s) 55, 58, 307, 461
BshFI GGCC 1 cut(s) 465
BsiHKAI GWGCWC 1 cut(s) 316
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 1 cut(s) 161
BsmAI GTCTC 4 cut(s) 229, 267, 364, 590
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 1 cut(s) 465
Bso31I GGTCTC 2 cut(s) 229, 590
Bsp1286I GDGCHC 1 cut(s) 316
BspANI GGCC 1 cut(s) 465
BspCNI CTCAG 4 cut(s) 82, 206, 319, 445
BspLI GGNNCC 2 cut(s) 166, 362
BspQI GCTCTTC 1 cut(s) 237
BspTNI GGTCTC 2 cut(s) 229, 590
BsrI ACTGG 2 cut(s) 295, 331
Bst2UI CCWGG 3 cut(s) 105, 156, 543
Bst4CI ACNGT 1 cut(s) 526
Bst6I CTCTTC 2 cut(s) 173, 237
BstDEI CTNAG 4 cut(s) 90, 193, 306, 453
BstMAI GTCTC 4 cut(s) 229, 267, 364, 590
BstMWI GCNNNNNNNGC 3 cut(s) 52, 211, 320
BstNI CCWGG 3 cut(s) 105, 156, 543
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 103, 154, 541
BstV1I GCAGC 4 cut(s) 55, 58, 307, 461
BstXI CCANNNNNNTGG 1 cut(s) 403
BsuRI GGCC 1 cut(s) 465
BtsIMutI CAGTG 3 cut(s) 314, 324, 573
Cfr13I GGNCC 3 cut(s) 360, 539, 545
CsiI ACCWGGT 1 cut(s) 154
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 2 cut(s) 317, 331
CviJI RGCY 8 cut(s) 88, 120, 205, 247, 354, 439, 452, 465
CviKI_1 RGCY 8 cut(s) 88, 120, 205, 247, 354, 439, 452, 465
DdeI CTNAG 4 cut(s) 90, 193, 306, 453
Eam1104I CTCTTC 2 cut(s) 173, 237
EarI CTCTTC 2 cut(s) 173, 237
Eco31I GGTCTC 2 cut(s) 229, 590
Eco47I GGWCC 3 cut(s) 360, 539, 545
EcoO109I RGGNCCY 1 cut(s) 360
EcoRII CCWGG 3 cut(s) 103, 154, 541
FaeI CATG 2 cut(s) 320, 334
FaiI YATR 4 cut(s) 318, 332, 336, 357
FalI AAGNNNNNCTT 4 cut(s) 7, 39, 152, 184
FaqI GGGAC 1 cut(s) 346
FatI CATG 2 cut(s) 316, 330
Fnu4HI GCNGC 4 cut(s) 44, 47, 321, 450
Fsp4HI GCNGC 4 cut(s) 44, 47, 321, 450
GluI GCNGC 4 cut(s) 44, 47, 321, 450
HaeIII GGCC 1 cut(s) 465
Hin1II CATG 2 cut(s) 320, 334
HinfI GANTC 3 cut(s) 31, 418, 563
HphI GGTGA 1 cut(s) 248
Hpy188I TCNGA 2 cut(s) 196, 593
Hpy188III TCNNGA 2 cut(s) 15, 226
HpyAV CCTTC 2 cut(s) 110, 476
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4V TGCA 4 cut(s) 338, 349, 414, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 211, 320
HpyF3I CTNAG 4 cut(s) 90, 193, 306, 453
Hsp92II CATG 2 cut(s) 320, 334
LguI GCTCTTC 1 cut(s) 237
Lsp1109I GCAGC 4 cut(s) 55, 58, 307, 461
LweI GCATC 3 cut(s) 311, 336, 478
MabI ACCWGGT 1 cut(s) 154
MaeIII GTNAC 2 cut(s) 406, 568
MboII GAAGA 3 cut(s) 15, 190, 254
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 3 cut(s) 18, 94, 187
MmeI TCCRAC 3 cut(s) 120, 494, 517
MnlI CCTC 4 cut(s) 174, 190, 373, 448
MroXI GAANNNNTTC 2 cut(s) 22, 188
MseI TTAA 4 cut(s) 71, 84, 249, 278
MslI CAYNNNNRTG 2 cut(s) 307, 329
MspA1I CMGCKG 1 cut(s) 452
MspR9I CCNGG 3 cut(s) 105, 156, 543
MvaI CCWGG 3 cut(s) 105, 156, 543
MwoI GCNNNNNNNGC 3 cut(s) 52, 211, 320
NlaIII CATG 2 cut(s) 320, 334
NlaIV GGNNCC 2 cut(s) 166, 362
NmuCI GTSAC 1 cut(s) 568
NspI RCATGY 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 237
PdmI GAANNNNTTC 2 cut(s) 22, 188
PfeI GAWTC 3 cut(s) 31, 418, 563
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 4 cut(s) 45, 48, 322, 451
PpuMI RGGWCCY 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 360
Psp6I CCWGG 3 cut(s) 103, 154, 541
PspGI CCWGG 3 cut(s) 103, 154, 541
PspN4I GGNNCC 2 cut(s) 166, 362
PspPI GGNCC 3 cut(s) 360, 539, 545
PspPPI RGGWCCY 1 cut(s) 360
PvuII CAGCTG 1 cut(s) 452
RseI CAYNNNNRTG 2 cut(s) 307, 329
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 4 cut(s) 71, 84, 249, 278
SatI GCNGC 4 cut(s) 44, 47, 321, 450
Sau96I GGNCC 3 cut(s) 360, 539, 545
ScrFI CCNGG 3 cut(s) 105, 156, 543
SduI GDGCHC 1 cut(s) 316
SetI ASST 6 cut(s) 157, 249, 362, 441, 454, 507
SexAI ACCWGGT 1 cut(s) 154
SfaNI GCATC 3 cut(s) 311, 336, 478
SinI GGWCC 3 cut(s) 360, 539, 545
SmiMI CAYNNNNRTG 2 cut(s) 307, 329
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 3 cut(s) 18, 94, 187
StyD4I CCNGG 3 cut(s) 103, 154, 541
TaaI ACNGT 1 cut(s) 526
TaqI TCGA 1 cut(s) 227
TasI AATT 3 cut(s) 18, 94, 187
TfiI GAWTC 3 cut(s) 31, 418, 563
Tru1I TTAA 4 cut(s) 71, 84, 249, 278
Tru9I TTAA 4 cut(s) 71, 84, 249, 278
TscAI CASTG 3 cut(s) 314, 331, 573
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 4 cut(s) 43, 46, 320, 449
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 3 cut(s) 112, 255, 446
TspRI CASTG 3 cut(s) 314, 331, 573
VpaK11BI GGWCC 3 cut(s) 360, 539, 545
XapI RAATTY 3 cut(s) 18, 94, 187
XceI RCATGY 1 cut(s) 334
XmnI GAANNNNTTC 2 cut(s) 22, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.