Rorug06G0349000

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007778
Physical Location & Seq
Forward (+)
49910619 .. 49911549
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0349000.1

Sequence Viewer

Length: 345 bp
ATGGCAAAGTCCTCTTCCATTATTCTCTTCTTCACCTTCTGCTTGTTCTTCACAGCCGTGATCATCGATGCAAGCAATGGCAGCGATCACGGGTTGAGCTTCGTTCCGGCCAAGGCCCGCTGCCAGGGTTCCATTGCAGAGTGCATGGCTGAAGATGAGTTTGAGATGGACTCTGAGATCAACAGGCGTATCTTGGCCACCACAAAGTACATCAGCTATGGAGCTCTGCAGAGGAACACTGTGCCATGCTCTCAGAGGGGTGCTTCCTACTACAACTGCAAGCCCGGCGCACAGGCAAACCCCTACAACCGTGGCTGCAGTGCCATCACTCGCTGCCGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

114

Amino Acids

12.51

Weight (kDa)

8.52

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 53 - 113 4.4e-31 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 118
AcoI YGGCCR 2 cut(s) 108, 195
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 124
AjnI CCWGG 1 cut(s) 123
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 3 cut(s) 99, 216, 224
AluI AGCT 3 cut(s) 99, 216, 224
Alw21I GWGCWC 1 cut(s) 226
AoxI GGCC 3 cut(s) 108, 114, 195
ApeKI GCWGC 4 cut(s) 81, 120, 315, 333
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 115
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 25
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BalI TGGCCA 1 cut(s) 197
BanII GRGCYC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 4 cut(s) 93, 107, 302, 320
BccI CCATC 2 cut(s) 160, 332
BceAI ACGGC 2 cut(s) 41, 321
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 1 cut(s) 285
BfmI CTRYAG 2 cut(s) 227, 316
BisI GCNGC 4 cut(s) 82, 121, 316, 334
BlsI GCNGC 4 cut(s) 83, 122, 317, 335
Bme1390I CCNGG 2 cut(s) 125, 285
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 2 cut(s) 125, 285
BmsI GCATC 1 cut(s) 58
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpuMI CCSGG 1 cut(s) 285
Bsa29I ATCGAT 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 111, 124, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse3DI GCAATG 2 cut(s) 82, 132
BseBI CCWGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 3 cut(s) 111, 124, 310
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 2 cut(s) 82, 132
BseMII CTCAG 2 cut(s) 165, 266
BseXI GCAGC 4 cut(s) 93, 107, 302, 320
BshFI GGCC 3 cut(s) 110, 116, 197
BshVI ATCGAT 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 2 cut(s) 107, 285
BslI CCNNNNNNNGG 1 cut(s) 124
BsnI GGCC 3 cut(s) 110, 116, 197
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 3 cut(s) 60, 85, 177
BspACI CCGC 1 cut(s) 118
BspANI GGCC 3 cut(s) 110, 116, 197
BspCNI CTCAG 2 cut(s) 166, 265
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 2 cut(s) 231, 320
BsrDI GCAATG 2 cut(s) 82, 132
BssECI CCNNGG 3 cut(s) 111, 124, 310
BssMI GATC 3 cut(s) 60, 85, 177
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 241, 311
Bst6I CTCTTC 2 cut(s) 19, 32
BstC8I GCNNGC 3 cut(s) 73, 118, 281
BstDEI CTNAG 2 cut(s) 174, 252
BstDSI CCRYGG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 3 cut(s) 63, 88, 180
BstMBI GATC 3 cut(s) 60, 85, 177
BstMWI GCNNNNNNNGC 2 cut(s) 81, 285
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 123, 283
BstSFI CTRYAG 2 cut(s) 227, 316
BstV1I GCAGC 4 cut(s) 93, 107, 302, 320
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 110, 116, 197
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 237, 325
Cac8I GCNNGC 3 cut(s) 73, 118, 281
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 1 cut(s) 208
CviAII CATG 2 cut(s) 145, 246
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 174, 252
DpnI GATC 3 cut(s) 62, 87, 179
DpnII GATC 3 cut(s) 60, 85, 177
EaeI YGGCCR 2 cut(s) 108, 195
Eam1104I CTCTTC 2 cut(s) 19, 32
EarI CTCTTC 2 cut(s) 19, 32
Ecl136II GAGCTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 148, 249
FaiI YATR 3 cut(s) 146, 219, 247
FatI CATG 2 cut(s) 144, 245
FauI CCCGC 1 cut(s) 125
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 82, 121, 316, 334
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 4 cut(s) 82, 121, 316, 334
GlaI GCGC 1 cut(s) 289
GluI GCNGC 4 cut(s) 82, 121, 316, 334
HaeIII GGCC 3 cut(s) 110, 116, 197
HapII CCGG 2 cut(s) 107, 285
HhaI GCGC 1 cut(s) 290
Hin1II CATG 2 cut(s) 148, 249
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 1 cut(s) 170
HpaII CCGG 2 cut(s) 107, 285
HphI GGTGA 1 cut(s) 25
Hpy188I TCNGA 2 cut(s) 175, 255
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 241, 311
HpyCH4V TGCA 6 cut(s) 71, 137, 144, 229, 279, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 285
HpyF3I CTNAG 2 cut(s) 174, 252
Hsp92II CATG 2 cut(s) 148, 249
HspAI GCGC 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 85, 177
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 110, 120, 137, 169, 278, 298
Lsp1109I GCAGC 4 cut(s) 93, 107, 302, 320
LweI GCATC 1 cut(s) 58
MalI GATC 3 cut(s) 62, 87, 179
MboI GATC 3 cut(s) 60, 85, 177
MboII GAAGA 5 cut(s) 6, 19, 22, 40, 164
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 3 cut(s) 22, 225, 249
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 2 cut(s) 107, 285
MspR9I CCNGG 2 cut(s) 125, 285
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 81, 285
NciI CCSGG 1 cut(s) 285
NdeII GATC 3 cut(s) 60, 85, 177
NlaIII CATG 2 cut(s) 148, 249
NlaIV GGNNCC 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 56
PkrI GCNGC 4 cut(s) 83, 122, 317, 335
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 2 cut(s) 231, 320
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 226
SatI GCNGC 4 cut(s) 82, 121, 316, 334
Sau3AI GATC 3 cut(s) 60, 85, 177
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 2 cut(s) 125, 285
SduI GDGCHC 1 cut(s) 226
SetI ASST 4 cut(s) 38, 101, 218, 226
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 2 cut(s) 227, 316
SmiMI CAYNNNNRTG 1 cut(s) 56
SsiI CCGC 1 cut(s) 118
SstI GAGCTC 1 cut(s) 226
StyD4I CCNGG 2 cut(s) 123, 283
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 241, 311
TaqI TCGA 1 cut(s) 66
TatI WGTACW 1 cut(s) 207
TscAI CASTG 2 cut(s) 244, 325
TseI GCWGC 4 cut(s) 81, 120, 315, 333
TspRI CASTG 2 cut(s) 244, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.