AT4G37030

membrane protein At3g27390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007093
Physical Location & Seq
Forward (+)
17451637 .. 17454760
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37030.1

Sequence Viewer

Length: 1710 bp
ATGGCTGTCGTCGTCACTAACCTAGGAAGCTGTCTGAAGATTTCTTATGTCATCTTTGCCTTTTGTTCTGCTTTCTTTCTCGGTGCTATCAAAGGTTTGATCGTAGGTCCAATTGCTGGATTAACATTAATAGTTGGGAATGTTGGAGTGATTCTTTGTTTGTTCCCTGCACATGTTACTTGGACTATTTACGCTGTTGCAAAGACAAATAGATTTGACATTCCTCTGAAGGTAGCAATCTTAGTGGCACTTCCTGCATTGTTTGGGATATGGTTAGGTCTAAGTCTAGCAATTAGTGTTCTTGTTGGTGTTGGCTATGGATTCTTCACTCCTTGGATCTCTGCTTTTGAAGCCTTTAGACAAGACACTGAATCCAACAAGTTCTTCCACTGTCTTGTGGATGGAACTTGGGGAACCATCAAAGGGAGTTGCATTGTGGTTACTGATTTTGCAGATTTTTGTTACCATTCTTATCCACTTTACTTAAAGGAGTTACGCGAATCCCCGGTTTCAGATGAGCTTCAAACTCTTAGGTTGATTCATGTTCCAGGATGCATCATTGTTGGGATTCTAGGATTAGTCATTGATATCCCTCTTTTCACTGCAATCGCGGTTATTAAAAGCCCTTACCTTCTTCTCAAAGGCTGGTACCGACTAGCTCAAGACGCGATTAATCGAGAAGGACCATTTCTTGAAATAGCTTGTATACCAGTTGCTGGTTTGACAGTACTGTTATGGCCTATAGTTGTCATTGGTTTTATTTTGGTGACCATCTTCTCCAGCATCTTTGTAGGACTATATGGAGCAGTTGTTGTATTTCAGGAAAGGTCATTCAGAAGAGGAGTCTCTTATGTGATTGCAGTAGTTGGAGAATTTGATGAGTACACAAATGATTGGCTTTACCTTAGAGAAGGAACCATCTTCCCAAAGCCAAGATATCGGATGGGAAGAGGATCGTTTTCAAGTGAAGTATCTGTGATCGTTCACCCTTCAGATGTAACAAGAGTCAATAGTTCTGGTTCTGTCGATGCTCCAGCGATGCTAGTACCGAGCCTAGTTCACTCTGTATCTGTTAGAGAAGCAATACAAGAAGTGAGAATGGTTCAGATTTGGGAGCATATGATGGGGTGGTTTGAGATGCAAGGCAAAGAGCTACTAGATGCAGAAGTGTTAACACCAACTGATCTCTATGAGTCTTTAAAGGGCAGACACGGAAACGAATCATCGATTATCAATGTCGGGCTTCCTTCTTACGCTTTGTTGCATACATTGCTTAGCTCTATAAAAGCTGGTGTTCATGGTGTGCTTTTGCTTGATGGCTCGGAAGTGACACATTTGAATAGACCACAAGACAAGTTCTTGGACTGGGTCTTTAATCCAATAATGGTATTGAAAGATCAGATTCGAGCTCTTAAACTTGGAGAAAGTGAAGTTAAATACTTAGAGAAAGTTGTTCTCTTTGGAAACCATGAACAAAGGATGGAAGCTTGGGATAATCACAGTAACCCGCCTCAAGAAAACCTTCGAACTGCTCAAATCCAAGGAATCAGCAGAAGGATGATGGGAATGGTACGGAGCGTATCGAAGCTTCCGACTTATAGAAGAAGGTTCAGACAAGTGGTTAAGGCTCTAATTACTTATTATTCAGAGAAACAAGGCTTAAATCGAACCGGTTCCATGAGCTCTGGAGATTTCATTGAAGAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

569

Amino Acids

63.34

Weight (kDa)

7.2

Isoelectric Point (pI)

30.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 648
AccB7I CCANNNNNTGG 2 cut(s) 116, 716
AccI GTMKAC 1 cut(s) 706
AccII CGCG 3 cut(s) 498, 611, 668
AciI CCGC 2 cut(s) 611, 1508
AclWI GGATC 2 cut(s) 344, 961
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 3 cut(s) 56, 248, 975
AfaI GTAC 5 cut(s) 650, 729, 884, 1047, 1572
AfiI CCNNNNNNNGG 3 cut(s) 116, 423, 716
AflIII ACRYGT 1 cut(s) 172
AgeI ACCGGT 1 cut(s) 1670
AgsI TTSAA 7 cut(s) 350, 524, 695, 963, 1339, 1393, 1700
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
Alw21I GWGCWC 2 cut(s) 1411, 1685
Alw26I GTCTC 1 cut(s) 850
AlwI GGATC 2 cut(s) 344, 961
AlwNI CAGNNNCTG 1 cut(s) 716
AoxI GGCC 1 cut(s) 737
ApoI RAATTY 1 cut(s) 872
AseI ATTAAT 2 cut(s) 128, 672
AsiGI ACCGGT 1 cut(s) 1670
Asp700I GAANNNNTTC 2 cut(s) 1521, 1672
Asp718I GGTACC 1 cut(s) 648
AspA2I CCTAGG 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 107, 683
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 2 cut(s) 778, 977
AsuII TTCGAA 1 cut(s) 1525
AvaII GGWCC 2 cut(s) 107, 683
AvrII CCTAGG 1 cut(s) 22
BanI GGYRCC 1 cut(s) 648
BanII GRGCYC 2 cut(s) 1411, 1685
BarI GAAGNNNNNNTAC 2 cut(s) 1572, 1604
Bbv12I GWGCWC 2 cut(s) 1411, 1685
BccI CCATC 9 cut(s) 395, 425, 779, 926, 937, 1117, 1310, 1474, 1555
BciT130I CCWGG 1 cut(s) 549
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 850
BfaI CTAG 7 cut(s) 23, 287, 572, 656, 1043, 1055, 1157
BfmI CTRYAG 1 cut(s) 741
BlnI CCTAGG 1 cut(s) 22
BlpI GCTNAGC 1 cut(s) 1274
BmcAI AGTACT 1 cut(s) 729
Bme1390I CCNGG 2 cut(s) 506, 549
Bme18I GGWCC 2 cut(s) 107, 683
BmgT120I GGNCC 2 cut(s) 107, 683
BmiI GGNNCC 4 cut(s) 415, 650, 916, 1675
BmrFI CCNGG 2 cut(s) 506, 549
BmrI ACTGGG 1 cut(s) 1375
BmsI GCATC 7 cut(s) 542, 564, 792, 1018, 1029, 1128, 1150
BmuI ACTGGG 1 cut(s) 1375
BpmI CTGGAG 3 cut(s) 763, 1017, 1707
Bpu1102I GCTNAGC 1 cut(s) 1274
Bpu14I TTCGAA 1 cut(s) 1525
BpuEI CTTGAG 2 cut(s) 645, 1497
BpuMI CCSGG 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 1226
BsaJI CCNNGG 4 cut(s) 22, 332, 504, 1540
BsaWI WCCGGW 1 cut(s) 1670
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 423, 716
Bse118I RCCGGY 1 cut(s) 1670
Bse1I ACTGG 2 cut(s) 710, 1370
Bse3DI GCAATG 1 cut(s) 1268
BseBI CCWGG 1 cut(s) 549
BseCI ATCGAT 1 cut(s) 1226
BseDI CCNNGG 4 cut(s) 22, 332, 504, 1540
BseGI GGATG 5 cut(s) 406, 557, 948, 1485, 1563
BseLI CCNNNNNNNGG 3 cut(s) 116, 423, 716
BseMI GCAATG 1 cut(s) 1268
BseNI ACTGG 2 cut(s) 710, 1370
BseRI GAGGAG 1 cut(s) 855
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 3 cut(s) 498, 611, 668
BshFI GGCC 1 cut(s) 739
BshNI GGYRCC 1 cut(s) 648
BshTI ACCGGT 1 cut(s) 1670
BshVI ATCGAT 1 cut(s) 1226
BsiHKAI GWGCWC 2 cut(s) 1411, 1685
BsiSI CCGG 2 cut(s) 506, 1671
BslI CCNNNNNNNGG 3 cut(s) 116, 423, 716
BsmAI GTCTC 1 cut(s) 850
BsnI GGCC 1 cut(s) 739
Bsp119I TTCGAA 1 cut(s) 1525
Bsp1286I GDGCHC 2 cut(s) 1411, 1685
Bsp143I GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
Bsp1720I GCTNAGC 1 cut(s) 1274
BspACI CCGC 2 cut(s) 611, 1508
BspANI GGCC 1 cut(s) 739
BspDI ATCGAT 1 cut(s) 1226
BspFNI CGCG 3 cut(s) 498, 611, 668
BspLI GGNNCC 4 cut(s) 415, 650, 916, 1675
BspPI GGATC 2 cut(s) 344, 961
BspT104I TTCGAA 1 cut(s) 1525
BspT107I GGYRCC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 1268
BsrFI RCCGGY 1 cut(s) 1670
BsrI ACTGG 2 cut(s) 710, 1370
BssAI RCCGGY 1 cut(s) 1670
BssECI CCNNGG 4 cut(s) 22, 332, 504, 1540
BssMI GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
BssNAI GTATAC 1 cut(s) 707
BssT1I CCWWGG 3 cut(s) 22, 332, 1540
Bst1107I GTATAC 1 cut(s) 707
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 4 cut(s) 392, 727, 732, 1502
Bst6I CTCTTC 3 cut(s) 832, 943, 1695
BstAPI GCANNNNNTGC 2 cut(s) 254, 1270
BstBI TTCGAA 1 cut(s) 1525
BstDEI CTNAG 6 cut(s) 241, 281, 530, 905, 1274, 1441
BstEII GGTNACC 1 cut(s) 766
BstF5I GGATG 5 cut(s) 406, 557, 948, 1485, 1563
BstFNI CGCG 3 cut(s) 498, 611, 668
BstKTI GATC 6 cut(s) 102, 339, 956, 981, 1186, 1399
BstMAI GTCTC 1 cut(s) 850
BstMBI GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
BstMWI GCNNNNNNNGC 4 cut(s) 254, 350, 665, 1270
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 176
BstPI GGTNACC 1 cut(s) 766
BstSCI CCNGG 2 cut(s) 504, 547
BstSFI CTRYAG 1 cut(s) 741
BstUI CGCG 3 cut(s) 498, 611, 668
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
BstZ17I GTATAC 1 cut(s) 707
Bsu15I ATCGAT 1 cut(s) 1226
BsuRI GGCC 1 cut(s) 739
BsuTUI ATCGAT 1 cut(s) 1226
BtgZI GCGATG 1 cut(s) 1052
BtsCI GGATG 5 cut(s) 406, 557, 948, 1485, 1563
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 3 cut(s) 366, 388, 600
CaiI CAGNNNCTG 1 cut(s) 716
Cfr10I RCCGGY 1 cut(s) 1670
Cfr13I GGNCC 2 cut(s) 107, 683
ClaI ATCGAT 1 cut(s) 1226
CseI GACGC 1 cut(s) 674
Csp6I GTAC 5 cut(s) 649, 728, 883, 1046, 1571
CspAI ACCGGT 1 cut(s) 1670
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 5 cut(s) 173, 542, 1298, 1469, 1678
CviQI GTAC 5 cut(s) 649, 728, 883, 1046, 1571
DdeI CTNAG 6 cut(s) 241, 281, 530, 905, 1274, 1441
DpnI GATC 6 cut(s) 101, 338, 955, 980, 1185, 1398
DpnII GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
DraI TTTAAA 1 cut(s) 1200
Eam1104I CTCTTC 3 cut(s) 832, 943, 1695
EarI CTCTTC 3 cut(s) 832, 943, 1695
Ecl136II GAGCTC 2 cut(s) 1409, 1683
Eco130I CCWWGG 3 cut(s) 22, 332, 1540
Eco24I GRGCYC 2 cut(s) 1411, 1685
Eco32I GATATC 2 cut(s) 589, 938
Eco47I GGWCC 2 cut(s) 107, 683
Eco53kI GAGCTC 2 cut(s) 1409, 1683
Eco57I CTGAAG 3 cut(s) 56, 248, 975
Eco91I GGTNACC 1 cut(s) 766
EcoICRI GAGCTC 2 cut(s) 1409, 1683
EcoO65I GGTNACC 1 cut(s) 766
EcoRII CCWGG 1 cut(s) 547
EcoRV GATATC 2 cut(s) 589, 938
EcoT14I CCWWGG 3 cut(s) 22, 332, 1540
EcoT22I ATGCAT 1 cut(s) 557
EcoT38I GRGCYC 2 cut(s) 1411, 1685
ErhI CCWWGG 3 cut(s) 22, 332, 1540
FaeI CATG 5 cut(s) 176, 545, 1301, 1472, 1681
FalI AAGNNNNNCTT 2 cut(s) 1506, 1538
FatI CATG 5 cut(s) 172, 541, 1297, 1468, 1677
FauI CCCGC 1 cut(s) 1515
FauNDI CATATG 1 cut(s) 1119
FblI GTMKAC 1 cut(s) 706
FokI GGATG 5 cut(s) 413, 564, 955, 1492, 1570
FriOI GRGCYC 2 cut(s) 1411, 1685
FspBI CTAG 7 cut(s) 23, 287, 572, 656, 1043, 1055, 1157
GsuI CTGGAG 3 cut(s) 763, 1017, 1707
HaeIII GGCC 1 cut(s) 739
HapII CCGG 2 cut(s) 506, 1671
HgaI GACGC 1 cut(s) 674
Hin1II CATG 5 cut(s) 176, 545, 1301, 1472, 1681
HincII GTYRAC 1 cut(s) 1173
HindII GTYRAC 1 cut(s) 1173
HindIII AAGCTT 2 cut(s) 1485, 1586
HpaI GTTAAC 1 cut(s) 1173
HpaII CCGG 2 cut(s) 506, 1671
HphI GGTGA 2 cut(s) 778, 977
Hpy166II GTNNAC 5 cut(s) 707, 885, 985, 1060, 1173
Hpy188III TCNNGA 6 cut(s) 662, 677, 692, 821, 1514, 1686
Hpy8I GTNNAC 5 cut(s) 707, 885, 985, 1060, 1173
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 9 cut(s) 223, 641, 674, 905, 999, 1257, 1532, 1548, 1599
HpyCH4III ACNGT 4 cut(s) 392, 727, 732, 1502
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 350, 665, 1270
HpyF3I CTNAG 6 cut(s) 241, 281, 530, 905, 1274, 1441
Hsp92II CATG 5 cut(s) 176, 545, 1301, 1472, 1681
KpnI GGTACC 1 cut(s) 652
KspAI GTTAAC 1 cut(s) 1173
Kzo9I GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
LmnI GCTCC 4 cut(s) 803, 1036, 1114, 1575
LweI GCATC 7 cut(s) 542, 564, 792, 1018, 1029, 1128, 1150
MaeI CTAG 7 cut(s) 23, 287, 572, 656, 1043, 1055, 1157
MaeIII GTNAC 9 cut(s) 13, 175, 439, 461, 492, 766, 997, 1327, 1502
MalI GATC 6 cut(s) 101, 338, 955, 980, 1185, 1398
MboI GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
MboII GAAGA 9 cut(s) 49, 316, 376, 626, 766, 849, 913, 960, 1614
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 2 cut(s) 1411, 1685
MluCI AATT 4 cut(s) 111, 291, 872, 1632
MlyI GAGTC 3 cut(s) 852, 1014, 1202
MmeI TCCRAC 4 cut(s) 124, 399, 847, 1616
MnlI CCTC 6 cut(s) 234, 603, 833, 944, 1521, 1696
Mph1103I ATGCAT 1 cut(s) 557
MroXI GAANNNNTTC 2 cut(s) 1521, 1672
MspI CCGG 2 cut(s) 506, 1671
MspR9I CCNGG 2 cut(s) 506, 549
MunI CAATTG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 3 cut(s) 498, 611, 668
MwoI GCNNNNNNNGC 4 cut(s) 254, 350, 665, 1270
NciI CCSGG 1 cut(s) 506
NdeI CATATG 1 cut(s) 1119
NdeII GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
NlaIII CATG 5 cut(s) 176, 545, 1301, 1472, 1681
NlaIV GGNNCC 4 cut(s) 415, 650, 916, 1675
NmuCI GTSAC 3 cut(s) 13, 766, 1327
NsiI ATGCAT 1 cut(s) 557
NspI RCATGY 1 cut(s) 176
NspV TTCGAA 1 cut(s) 1525
PciI ACATGT 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 1589
PdmI GAANNNNTTC 2 cut(s) 1521, 1672
PfeI GAWTC 9 cut(s) 151, 321, 371, 500, 538, 568, 1220, 1402, 1545
PflFI GACNNNGTC 1 cut(s) 1367
PflMI CCANNNNNTGG 2 cut(s) 116, 716
PfoI TCCNGGA 1 cut(s) 547
PinAI ACCGGT 1 cut(s) 1670
PleI GAGTC 3 cut(s) 851, 1013, 1201
PpsI GAGTC 3 cut(s) 851, 1013, 1201
PscI ACATGT 1 cut(s) 172
PshBI ATTAAT 2 cut(s) 128, 672
Psp124BI GAGCTC 2 cut(s) 1411, 1685
Psp6I CCWGG 1 cut(s) 547
PspEI GGTNACC 1 cut(s) 766
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 4 cut(s) 415, 650, 916, 1675
PspPI GGNCC 2 cut(s) 107, 683
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 1 cut(s) 336
PsyI GACNNNGTC 1 cut(s) 1367
RsaI GTAC 5 cut(s) 650, 729, 884, 1047, 1572
RsaNI GTAC 5 cut(s) 649, 728, 883, 1046, 1571
SacI GAGCTC 2 cut(s) 1411, 1685
Sau3AI GATC 6 cut(s) 99, 336, 953, 978, 1183, 1396
Sau96I GGNCC 2 cut(s) 107, 683
ScaI AGTACT 1 cut(s) 729
SchI GAGTC 3 cut(s) 852, 1014, 1202
ScrFI CCNGG 2 cut(s) 506, 549
SduI GDGCHC 2 cut(s) 1411, 1685
SfaNI GCATC 7 cut(s) 542, 564, 792, 1018, 1029, 1128, 1150
SfcI CTRYAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 1525
SinI GGWCC 2 cut(s) 107, 683
SmlI CTYRAG 2 cut(s) 660, 1512
SmoI CTYRAG 2 cut(s) 660, 1512
Sse9I AATT 4 cut(s) 111, 291, 872, 1632
SsiI CCGC 2 cut(s) 611, 1508
SspMI CTAG 7 cut(s) 23, 287, 572, 656, 1043, 1055, 1157
SstI GAGCTC 2 cut(s) 1411, 1685
StyD4I CCNGG 2 cut(s) 504, 547
StyI CCWWGG 3 cut(s) 22, 332, 1540
TaaI ACNGT 4 cut(s) 392, 727, 732, 1502
TaqI TCGA 7 cut(s) 676, 1026, 1226, 1405, 1525, 1583, 1666
TasI AATT 4 cut(s) 111, 291, 872, 1632
TatI WGTACW 2 cut(s) 727, 882
TfiI GAWTC 9 cut(s) 151, 321, 371, 500, 538, 568, 1220, 1402, 1545
TscAI CASTG 3 cut(s) 373, 395, 607
TseFI GTSAC 3 cut(s) 13, 766, 1327
Tsp45I GTSAC 3 cut(s) 13, 766, 1327
TspDTI ATGAA 4 cut(s) 530, 1286, 1485, 1684
TspGWI ACGGA 2 cut(s) 1227, 1588
TspRI CASTG 3 cut(s) 373, 395, 607
Tth111I GACNNNGTC 1 cut(s) 1367
Van91I CCANNNNNTGG 2 cut(s) 116, 716
VpaK11BI GGWCC 2 cut(s) 107, 683
VspI ATTAAT 2 cut(s) 128, 672
XapI RAATTY 1 cut(s) 872
XceI RCATGY 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 706
XmnI GAANNNNTTC 2 cut(s) 1521, 1672
XspI CTAG 7 cut(s) 23, 287, 572, 656, 1043, 1055, 1157
ZrmI AGTACT 1 cut(s) 729
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.