FvH4_1g16000

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007093
Physical Location & Seq
Forward (+)
9099299 .. 9100025
727 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16000.t1

Sequence Viewer

Length: 312 bp
ATGGTTGCTGAGTTTGATGAGTACACAAATGACTGGTTATATCTTCGAGAGGGCACCATATTTCCGAAGTCACCAGGCCCCATTATCGGAAAACAGCTGTCTCTCAACCATCAGAGCTTTCTGTCCGAGGAAGGCAAGCCGCAAGCAGCTGGAGGTAAGATCATTTATGCTCAAATGGAGGCACCTGCAATGCATATACCACGTTTGGCCAATACAAGATCAGTTAGAGAGTCTATACATCAAGTAAAAATAGTACAGGATGAATGGGATGACGAGAGGCGTCTTATAGGGGGAGATTTCACCAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

104

Amino Acids

11.73

Weight (kDa)

5.55

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 193
AccB1I GGYRCC 2 cut(s) 53, 181
AciI CCGC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 2 cut(s) 23, 255
AfiI CCNNNNNNNGG 1 cut(s) 86
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 3 cut(s) 97, 117, 149
AluI AGCT 3 cut(s) 97, 117, 149
Alw26I GTCTC 1 cut(s) 105
AoxI GGCC 2 cut(s) 76, 207
ApeKI GCWGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 63, 292
BaeGI GKGCMC 1 cut(s) 56
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 2 cut(s) 53, 181
BbvI GCAGC 1 cut(s) 158
BccI CCATC 1 cut(s) 117
BciT130I CCWGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 2 cut(s) 140, 147
BlsI GCNGC 2 cut(s) 141, 148
Bme1390I CCNGG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 3 cut(s) 55, 79, 183
BmrFI CCNGG 1 cut(s) 75
BpmI CTGGAG 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 265, 274
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 1 cut(s) 158
BshFI GGCC 2 cut(s) 78, 209
BshNI GGYRCC 2 cut(s) 53, 181
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 105
BsnI GGCC 2 cut(s) 78, 209
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 2 cut(s) 159, 218
BspACI CCGC 1 cut(s) 140
BspANI GGCC 2 cut(s) 78, 209
BspLI GGNNCC 3 cut(s) 55, 79, 183
BspMI ACCTGC 1 cut(s) 193
BspT107I GGYRCC 2 cut(s) 53, 181
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 159, 218
BssNI GRCGYC 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 137, 144
BstDEI CTNAG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 265, 274
BstKTI GATC 2 cut(s) 162, 221
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 2 cut(s) 159, 218
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 73
BstSLI GKGCMC 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 158
BsuRI GGCC 2 cut(s) 78, 209
BtsCI GGATG 2 cut(s) 265, 274
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 137, 144
Cfr13I GGNCC 1 cut(s) 77
CseI GACGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 22, 254
CviJI RGCY 6 cut(s) 78, 97, 117, 139, 149, 209
CviKI_1 RGCY 6 cut(s) 78, 97, 117, 139, 149, 209
CviQI GTAC 2 cut(s) 22, 254
DdeI CTNAG 1 cut(s) 9
DpnI GATC 2 cut(s) 161, 220
DpnII GATC 2 cut(s) 159, 218
EaeI YGGCCR 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 195
FaiI YATR 7 cut(s) 40, 59, 168, 195, 197, 236, 287
Fnu4HI GCNGC 2 cut(s) 140, 147
FokI GGATG 2 cut(s) 272, 281
Fsp4HI GCNGC 2 cut(s) 140, 147
GluI GCNGC 2 cut(s) 140, 147
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 78, 209
HgaI GACGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 280
HinfI GANTC 1 cut(s) 230
HphI GGTGA 2 cut(s) 63, 292
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 4 cut(s) 66, 89, 114, 127
Hpy188III TCNNGA 1 cut(s) 47
Hpy8I GTNNAC 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 125
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 188, 193
HpyF3I CTNAG 1 cut(s) 9
HpySE526I ACGT 1 cut(s) 202
Hsp92I GRCGYC 1 cut(s) 280
Kzo9I GATC 2 cut(s) 159, 218
LpnPI CCDG 6 cut(s) 19, 60, 87, 135, 198, 242
Lsp1109I GCAGC 1 cut(s) 158
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 1 cut(s) 69
MalI GATC 2 cut(s) 161, 220
MboI GATC 2 cut(s) 159, 218
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 56
MlsI TGGCCA 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 5 cut(s) 43, 121, 146, 172, 270
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 195
MscI TGGCCA 1 cut(s) 209
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 2 cut(s) 97, 149
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
NdeII GATC 2 cut(s) 159, 218
NlaIV GGNNCC 3 cut(s) 55, 79, 183
NmuCI GTSAC 1 cut(s) 69
NsiI ATGCAT 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 193
PkrI GCNGC 2 cut(s) 141, 148
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 3 cut(s) 55, 79, 183
PspPI GGNCC 1 cut(s) 77
PvuII CAGCTG 2 cut(s) 97, 149
RsaI GTAC 2 cut(s) 23, 255
RsaNI GTAC 2 cut(s) 22, 254
SatI GCNGC 2 cut(s) 140, 147
Sau3AI GATC 2 cut(s) 159, 218
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 56
SetI ASST 6 cut(s) 99, 119, 151, 157, 187, 205
SsiI CCGC 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 73
TaiI ACGT 1 cut(s) 205
TaqI TCGA 1 cut(s) 46
TatI WGTACW 2 cut(s) 21, 253
TauI GCSGC 1 cut(s) 142
TseFI GTSAC 1 cut(s) 69
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 69
TspDTI ATGAA 1 cut(s) 276
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.