Rh2CG182000

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007093
Physical Location & Seq
Reverse (-)
16742358 .. 16745761
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG182000.1

Sequence Viewer

Length: 1668 bp
ATGGATTTGTCACAAACTTGGAGCGCTGGGCTGAAAGCTCTCTATGTGGTGTTTGCTTTCTGCTCTGCTCTCTTTCTGGGTTTTCTCAAAGGTGTTCTTGTGGGTCCTGTTTCTGCTCTAATAATGATTATAGGGAATGTGGGAATCATATTGGGCTTGTTTCCTGCACATGTTGCCTGGACAATCTACACTCTTATAAAGATTGATAGATTTGATGTACCACTTAAAGTTGCAATATTGTTTGGCTTGCCAGCCCTGTTTGGTATCTGGCTGGGTTTGAGCATAGCTGGGAGTGTTCTTGTGGGATTGGGCTATGGGTTTTTCACTCCTTGGGTTTCTACTTTTGAGGCTTTTAGGCATGGCAATGAGTTGAAGAAGTTCAAACATTGTGTTATTGATGGAACTTGGGGAACTGTTAAAGGAAGCTGTACTGTAGTTAGAGACTTTGCAGACATATGTTACCACTCGTACCCGATCTACTTGAAGGAGTTGCGTGAATCCCCTGCTTCAGAAGAAGTTAAAACTCTAAGGTTGATTCATGTACCAGCATGTATACTAGTTGGTGTGTTGGGGCTAATGGTGGAGATTCCTCTTTACACTGCAATTGCTGTTGTGAAGAGTCCATATATGCTGTTTAAGGGCTGGTATCGACTGATTCATGATCTGATTAGTCGAGAAGGACCATTTCTTGAAGTAGCTTGCATCCCTATTGCTGGTTTGACAATCCTCATGTGGCCAATTGTGGTTGTTGGAAGCATCTTACTGACACTTGTCTCCAGCATTTTTGTTGGATTATATGCAGCAGTGGTAGTATATCAGGAAAGATCTTTCAAGAGAGGTGTGACTTACGTGATTGCAATGGTTGCTGAGTTTGATGAGTACACAAATGACTGGTTATATCTTCGAGAGGGCACCATCTTTCCGAAGCCCCATTATCGAAAAAAAGTTGTTTCTCAACCATCAGAGCTTTCTGTCCGAGGAAGGCTGGCAGCTGGAGGTAAGCTCAGTTCTGCTCCAATGGAGGCACCTGCAATGCATATGCCAAGTGCGGCCAATTCAAGATCTGTTAGAGAGGCTATACATGAAGTAAAAATGGTACAGATATGGGGACATATGATGAGATCCTGCGAACTGAGGGCTAAGCAACTGTTGGATGCAGAAGTAATAACCCCAAGTGATCTCTATGAATGGTTGAAATCTGAGAACAGCAATGAAGGGTGCATCATTGGTGTTGGTTTGCCTTGTTATTCATTCTTGCACACACTTCTCTGCTCCATCAGATCAAATTCGGCTGGTTTGCTGCTGCTTGATGATGTTGAAGTTACCTATTTGAATAGACCACATGACAAACTGATGGACTGGTTCTTTAATCCTATAATGGTCCTGAAGGAACAGGTCAAGGTCATAAAATTGGAAGAGAGTGAGGAGAGATTTCTGGAGAAAGTACTACTTTTCAGAAGCAATACACAGCGCATGGAGGCTTGGGATAATGGAAGTTTAATACCTCAAGATGCTCTAAGAGCTGCTCAAATCGAAGGCATTAGTAGAAGGTATGAAGTTTTTGCTTATGTTCCAATTCCATCCAACCAATTAAAAAAAGAAAACAAGAATTCCTTTTGTGATTCAGCACATTCATGTTATGAATTGGTGAATGCAATTGTAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

555

Amino Acids

62.09

Weight (kDa)

7.55

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
AarI CACCTGC 1 cut(s) 1036
Acc36I ACCTGC 1 cut(s) 1036
AccB1I GGYRCC 2 cut(s) 911, 1024
AccI GTMKAC 1 cut(s) 553
AciI CCGC 1 cut(s) 1049
AclWI GGATC 1 cut(s) 1116
AcoI YGGCCR 2 cut(s) 734, 1050
AcsI RAATTY 2 cut(s) 1285, 1609
AcuI CTGAAG 2 cut(s) 492, 1406
AfaI GTAC 7 cut(s) 219, 430, 470, 543, 881, 1098, 1446
AfeI AGCGCT 1 cut(s) 25
AfiI CCNNNNNNNGG 1 cut(s) 713
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 9 cut(s) 373, 382, 484, 692, 832, 1059, 1195, 1319, 1333
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 8 cut(s) 38, 287, 426, 698, 967, 992, 1003, 1523
AluI AGCT 8 cut(s) 38, 287, 426, 698, 967, 992, 1003, 1523
Alw26I GTCTC 2 cut(s) 435, 778
AlwI GGATC 1 cut(s) 1116
Aor51HI AGCGCT 1 cut(s) 25
AoxI GGCC 2 cut(s) 734, 1050
ApeKI GCWGC 5 cut(s) 800, 989, 1300, 1303, 1523
ApoI RAATTY 2 cut(s) 1285, 1609
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 2 cut(s) 26, 1473
AspS9I GGNCC 3 cut(s) 104, 680, 1381
AsuHPI GGTGA 1 cut(s) 1660
AvaII GGWCC 3 cut(s) 104, 680, 1381
BaeGI GKGCMC 1 cut(s) 914
BalI TGGCCA 1 cut(s) 736
BanI GGYRCC 2 cut(s) 911, 1024
BbvI GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
BccI CCATC 6 cut(s) 392, 923, 967, 1283, 1348, 1588
BciT130I CCWGG 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 435, 778
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 1036
BglII AGATCT 2 cut(s) 824, 1061
BisI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
BlpI GCTNAGC 1 cut(s) 1140
BlsI GCNGC 6 cut(s) 802, 991, 1051, 1302, 1305, 1525
BmcAI AGTACT 1 cut(s) 1446
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 3 cut(s) 104, 680, 1381
BmgT120I GGNCC 3 cut(s) 104, 680, 1381
BmiI GGNNCC 3 cut(s) 105, 913, 1026
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 5 cut(s) 711, 765, 1144, 1230, 1501
BoxI GACNNNNGTC 1 cut(s) 770
BplI GAGNNNNNCTC 2 cut(s) 987, 1019
BpmI CTGGAG 3 cut(s) 760, 1014, 1457
Bpu1102I GCTNAGC 1 cut(s) 1140
BpuEI CTTGAG 1 cut(s) 1491
BsaAI YACGTR 1 cut(s) 850
BsaJI CCNNGG 2 cut(s) 329, 976
Bsc4I CCNNNNNNNGG 1 cut(s) 713
Bse1I ACTGG 2 cut(s) 896, 1364
Bse3DI GCAATG 4 cut(s) 370, 864, 1038, 1216
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 2 cut(s) 329, 976
BseGI GGATG 3 cut(s) 702, 1159, 1580
BseLI CCNNNNNNNGG 1 cut(s) 713
BseMI GCAATG 4 cut(s) 370, 864, 1038, 1216
BseMII CTCAG 4 cut(s) 858, 1018, 1124, 1191
BseNI ACTGG 2 cut(s) 896, 1364
BseRI GAGGAG 1 cut(s) 1439
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
BseYI CCCAGC 3 cut(s) 26, 271, 287
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 2 cut(s) 736, 1052
BshNI GGYRCC 2 cut(s) 911, 1024
BslFI GGGAC 1 cut(s) 1122
BslI CCNNNNNNNGG 1 cut(s) 713
BsmAI GTCTC 2 cut(s) 435, 778
BsmFI GGGAC 1 cut(s) 1122
BsmI GAATGC 1 cut(s) 1657
BsnI GGCC 2 cut(s) 736, 1052
Bsp1286I GDGCHC 1 cut(s) 914
Bsp143I GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
Bsp1720I GCTNAGC 1 cut(s) 1140
BspACI CCGC 1 cut(s) 1049
BspANI GGCC 2 cut(s) 736, 1052
BspCNI CTCAG 4 cut(s) 859, 1017, 1125, 1192
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 3 cut(s) 105, 913, 1026
BspMI ACCTGC 1 cut(s) 1036
BspPI GGATC 1 cut(s) 1116
BspT107I GGYRCC 2 cut(s) 911, 1024
BsrDI GCAATG 4 cut(s) 370, 864, 1038, 1216
BsrI ACTGG 2 cut(s) 896, 1364
BssECI CCNNGG 2 cut(s) 329, 976
BssMI GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
BssNAI GTATAC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 329
Bst1107I GTATAC 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 3 cut(s) 415, 433, 1149
Bst6I CTCTTC 2 cut(s) 611, 1410
BstAPI GCANNNNNTGC 2 cut(s) 173, 863
BstBAI YACGTR 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 248, 252, 700, 987
BstDEI CTNAG 7 cut(s) 527, 867, 1004, 1133, 1140, 1200, 1517
BstF5I GGATG 3 cut(s) 702, 1159, 1580
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 1473
BstKTI GATC 7 cut(s) 477, 664, 827, 1064, 1124, 1180, 1283
BstMAI GTCTC 2 cut(s) 435, 778
BstMBI GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
BstMWI GCNNNNNNNGC 3 cut(s) 173, 863, 1520
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 2 cut(s) 173, 552
BstPAI GACNNNNGTC 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 914
BstV1I GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
BstX2I RGATCY 3 cut(s) 824, 1061, 1121
BstYI RGATCY 3 cut(s) 824, 1061, 1121
BstZ17I GTATAC 1 cut(s) 554
BsuRI GGCC 2 cut(s) 736, 1052
BtsCI GGATG 3 cut(s) 702, 1159, 1580
BtsI GCAGTG 2 cut(s) 597, 810
BtsIMutI CAGTG 2 cut(s) 597, 810
BveI ACCTGC 1 cut(s) 1036
Cac8I GCNNGC 4 cut(s) 248, 252, 700, 987
CciI TCATGA 1 cut(s) 658
CfoI GCGC 2 cut(s) 26, 1473
Cfr13I GGNCC 3 cut(s) 104, 680, 1381
Csp6I GTAC 7 cut(s) 218, 429, 469, 542, 880, 1097, 1445
CviQI GTAC 7 cut(s) 218, 429, 469, 542, 880, 1097, 1445
DdeI CTNAG 7 cut(s) 527, 867, 1004, 1133, 1140, 1200, 1517
DpnI GATC 7 cut(s) 476, 663, 826, 1063, 1123, 1179, 1282
DpnII GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
EaeI YGGCCR 2 cut(s) 734, 1050
Eam1104I CTCTTC 2 cut(s) 611, 1410
EarI CTCTTC 2 cut(s) 611, 1410
Eco130I CCWWGG 1 cut(s) 329
Eco47I GGWCC 3 cut(s) 104, 680, 1381
Eco47III AGCGCT 1 cut(s) 25
Eco57I CTGAAG 2 cut(s) 492, 1406
EcoO109I RGGNCCY 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 1609
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 329
FalI AAGNNNNNCTT 6 cut(s) 81, 113, 1434, 1466, 1598, 1630
FaqI GGGAC 1 cut(s) 1122
FauNDI CATATG 3 cut(s) 455, 1038, 1113
FblI GTMKAC 1 cut(s) 553
Fnu4HI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
FokI GGATG 3 cut(s) 689, 1166, 1567
Fsp4HI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
FspBI CTAG 1 cut(s) 557
GlaI GCGC 2 cut(s) 25, 1472
GluI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
GsaI CCCAGC 3 cut(s) 30, 275, 291
GsuI CTGGAG 3 cut(s) 760, 1014, 1457
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 2 cut(s) 736, 1052
HhaI GCGC 2 cut(s) 26, 1473
Hin6I GCGC 2 cut(s) 24, 1471
HinP1I GCGC 2 cut(s) 24, 1471
HinfI GANTC 7 cut(s) 144, 497, 535, 586, 619, 655, 1622
HphI GGTGA 1 cut(s) 1660
Hpy166II GTNNAC 2 cut(s) 554, 882
Hpy188I TCNGA 8 cut(s) 511, 666, 924, 964, 977, 1201, 1280, 1457
Hpy8I GTNNAC 2 cut(s) 554, 882
HpyAV CCTTC 7 cut(s) 478, 671, 975, 1208, 1381, 1529, 1542
HpyCH4III ACNGT 3 cut(s) 415, 433, 1149
HpyCH4IV ACGT 1 cut(s) 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 863, 1520
HpyF3I CTNAG 7 cut(s) 527, 867, 1004, 1133, 1140, 1200, 1517
HpySE526I ACGT 1 cut(s) 849
HspAI GCGC 2 cut(s) 24, 1471
Kzo9I GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
LmnI GCTCC 3 cut(s) 21, 1018, 1277
Lsp1109I GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
LweI GCATC 5 cut(s) 711, 765, 1144, 1230, 1501
MaeI CTAG 1 cut(s) 557
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 4 cut(s) 9, 458, 841, 1321
MalI GATC 7 cut(s) 476, 663, 826, 1063, 1123, 1179, 1282
MboI GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
MboII GAAGA 5 cut(s) 385, 524, 628, 893, 1427
MfeI CAATTG 3 cut(s) 603, 738, 1656
MflI RGATCY 3 cut(s) 824, 1061, 1121
MhlI GDGCHC 1 cut(s) 914
MlsI TGGCCA 1 cut(s) 736
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 4 cut(s) 730, 769, 1131, 1608
Mox20I TGGCCA 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 1038
MroXI GAANNNNTTC 1 cut(s) 377
MscI TGGCCA 1 cut(s) 736
MseI TTAA 7 cut(s) 225, 417, 519, 636, 1368, 1499, 1592
MslI CAYNNNNRTG 2 cut(s) 363, 1633
Msp20I TGGCCA 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 992
MspR9I CCNGG 1 cut(s) 178
MunI CAATTG 3 cut(s) 603, 738, 1656
Mva1269I GAATGC 1 cut(s) 1657
MvaI CCWGG 1 cut(s) 178
MwoI GCNNNNNNNGC 3 cut(s) 173, 863, 1520
NdeI CATATG 3 cut(s) 455, 1038, 1113
NdeII GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
NlaIV GGNNCC 3 cut(s) 105, 913, 1026
NmuCI GTSAC 2 cut(s) 9, 841
NsiI ATGCAT 1 cut(s) 1038
NspI RCATGY 2 cut(s) 173, 552
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 1036
PciI ACATGT 1 cut(s) 169
PctI GAATGC 1 cut(s) 1657
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 6 cut(s) 144, 497, 535, 586, 655, 1622
PkrI GCNGC 6 cut(s) 802, 991, 1051, 1302, 1305, 1525
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
Ppu21I YACGTR 1 cut(s) 850
PpuMI RGGWCCY 1 cut(s) 104
PscI ACATGT 1 cut(s) 169
PshAI GACNNNNGTC 1 cut(s) 770
PsiI TTATAA 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 176
PspFI CCCAGC 3 cut(s) 26, 271, 287
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 105, 913, 1026
PspPI GGNCC 3 cut(s) 104, 680, 1381
PspPPI RGGWCCY 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 991, 1023
PsuI RGATCY 3 cut(s) 824, 1061, 1121
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 7 cut(s) 219, 430, 470, 543, 881, 1098, 1446
RsaNI GTAC 7 cut(s) 218, 429, 469, 542, 880, 1097, 1445
RseI CAYNNNNRTG 2 cut(s) 363, 1633
SaqAI TTAA 7 cut(s) 225, 417, 519, 636, 1368, 1499, 1592
SatI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
Sau3AI GATC 7 cut(s) 474, 661, 824, 1061, 1121, 1177, 1280
Sau96I GGNCC 3 cut(s) 104, 680, 1381
ScaI AGTACT 1 cut(s) 1446
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 5 cut(s) 711, 765, 1144, 1230, 1501
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 3 cut(s) 104, 680, 1381
SmiMI CAYNNNNRTG 2 cut(s) 363, 1633
SmlI CTYRAG 1 cut(s) 1506
SmoI CTYRAG 1 cut(s) 1506
SpeI ACTAGT 1 cut(s) 556
SsiI CCGC 1 cut(s) 1049
SspI AATATT 1 cut(s) 237
SspMI CTAG 1 cut(s) 557
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 3 cut(s) 415, 433, 1149
TaiI ACGT 1 cut(s) 852
TaqI TCGA 5 cut(s) 649, 673, 904, 937, 1533
TatI WGTACW 3 cut(s) 428, 879, 1444
TauI GCSGC 1 cut(s) 1052
TfiI GAWTC 6 cut(s) 144, 497, 535, 586, 655, 1622
Tru1I TTAA 7 cut(s) 225, 417, 519, 636, 1368, 1499, 1592
Tru9I TTAA 7 cut(s) 225, 417, 519, 636, 1368, 1499, 1592
TscAI CASTG 2 cut(s) 604, 810
TseFI GTSAC 2 cut(s) 9, 841
TseI GCWGC 5 cut(s) 800, 989, 1300, 1303, 1523
Tsp45I GTSAC 2 cut(s) 9, 841
TspDTI ATGAA 9 cut(s) 527, 647, 1098, 1200, 1227, 1239, 1569, 1623, 1656
TspRI CASTG 2 cut(s) 604, 810
VpaK11BI GGWCC 3 cut(s) 104, 680, 1381
XapI RAATTY 2 cut(s) 1285, 1609
XceI RCATGY 2 cut(s) 173, 552
XmiI GTMKAC 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 557
ZrmI AGTACT 1 cut(s) 1446
Zsp2I ATGCAT 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.