pycom02g13700

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007093
Physical Location & Seq
Reverse (-)
10313309 .. 10315244
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13700.2

Sequence Viewer

Length: 852 bp
ATGAGTACACGAATGACTGGCTGTATCTTCGAGAGGGAACAATCTTTCCAAGCCAGGCCCAAATATCGAAAAAGAGCTGTCTCTCAATCACCGGAATTCTCTGTTGGAGGAAATCAGCTGGATGAAGGTAAACCAAGTCCTGCTCGTATGGAAGCACCTGCTATGCTTATGCCAAGTGTAGCCCATTCAAGATCAGTTAGAGCCGTTATACAAGAAGTGAAAATGGTTCAGATATGGGAAAACATGATGAAATCCTGCGAACTGAGGGGCAGGGCATTGTTGGATGATAATGTAATAACAACAACTGACCTCGATGGATGGTTGAAAGCAAAGAACAGCAACGAAGCATCAATTATCGGTGTTGGCTTGCCTAGTTATTCATTCTTGCAGACTCTGCTCTGCTCCATCAGATCAAATTCAGATGGTTTACTGCTGCTGGATAACGTTGAAGTTAACTACATAAATAGACCGCATGACAAATTGTTGGACTGGTTCTTTAATCCGATAATGGTCCTGAAGGAACAGATTAAGGTCTTACAATTGGCGGAGGGTGAGGTTAGATATCTGGAGAAAGTGGTTCTTTTCGGAAGCAATAATCCACAACGCTTCGAGGCTTCGGATAGCGGAAGTTTACTACCTCAAGACGCTGTAAGAGCTGCTCAAATCGAAGGCATTAGTAGAAGGATGATTGGAATGATGAGAAGTATCTCAAAACTTCCCACTTACAGAAGGAAATTCCGGCACGTTGTCAAGGCTCTACTCGCATATTCCTTACCCAAGGAGGCTTCAATCGAAGCCGGTTCTGTTAAATCTACCATCTCCAGTGAAGATGTTGTGCTGGATGAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

31.75

Weight (kDa)

8.91

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 166
Acc36I ACCTGC 1 cut(s) 166
AciI CCGC 3 cut(s) 470, 545, 624
AclI AACGTT 1 cut(s) 444
AcsI RAATTY 3 cut(s) 95, 415, 734
AcuI CTGAAG 1 cut(s) 536
AfaI GTAC 1 cut(s) 7
AgsI TTSAA 4 cut(s) 189, 325, 449, 789
AjnI CCWGG 1 cut(s) 53
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AloI GAACNNNNNNTCC 2 cut(s) 30, 62
AluBI AGCT 3 cut(s) 77, 118, 656
AluI AGCT 3 cut(s) 77, 118, 656
Alw26I GTCTC 1 cut(s) 85
AlwNI CAGNNNCTG 1 cut(s) 394
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 2 cut(s) 433, 656
ApoI RAATTY 3 cut(s) 95, 415, 734
AspS9I GGNCC 2 cut(s) 57, 511
AsuHPI GGTGA 2 cut(s) 81, 563
AvaII GGWCC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 420, 643
BccI CCATC 5 cut(s) 308, 312, 413, 416, 824
BceAI ACGGC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 166
BisI GCNGC 2 cut(s) 434, 657
BlsI GCNGC 2 cut(s) 435, 658
Bme1390I CCNGG 1 cut(s) 55
Bme18I GGWCC 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 57, 511
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 1 cut(s) 356
BpmI CTGGAG 2 cut(s) 587, 805
BpuEI CTTGAG 1 cut(s) 624
BsaJI CCNNGG 1 cut(s) 777
BsaWI WCCGGW 1 cut(s) 91
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 3 cut(s) 22, 494, 822
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 5 cut(s) 127, 289, 323, 690, 847
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 3 cut(s) 22, 494, 822
BseXI GCAGC 2 cut(s) 420, 643
BshFI GGCC 1 cut(s) 58
BsiSI CCGG 3 cut(s) 92, 739, 798
BsmAI GTCTC 1 cut(s) 85
BsnI GGCC 1 cut(s) 58
Bsp143I GATC 2 cut(s) 191, 410
BspACI CCGC 3 cut(s) 470, 545, 624
BspANI GGCC 1 cut(s) 58
BspCNI CTCAG 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 3 cut(s) 22, 494, 822
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 2 cut(s) 191, 410
BssT1I CCWWGG 1 cut(s) 777
Bst2UI CCWGG 1 cut(s) 55
BstAPI GCANNNNNTGC 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 1 cut(s) 263
BstF5I GGATG 5 cut(s) 127, 289, 323, 690, 847
BstKTI GATC 2 cut(s) 194, 413
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 191, 410
BstMWI GCNNNNNNNGC 3 cut(s) 394, 653, 761
BstNI CCWGG 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 53
BstV1I GCAGC 2 cut(s) 420, 643
BsuRI GGCC 1 cut(s) 58
BtsCI GGATG 5 cut(s) 127, 289, 323, 690, 847
BtsIMutI CAGTG 1 cut(s) 829
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 368
CaiI CAGNNNCTG 1 cut(s) 394
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 2 cut(s) 57, 511
CseI GACGC 1 cut(s) 653
Csp6I GTAC 1 cut(s) 6
CviAII CATG 2 cut(s) 244, 473
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 263
DpnI GATC 2 cut(s) 193, 412
DpnII GATC 2 cut(s) 191, 410
EciI GGCGGA 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 777
Eco32I GATATC 1 cut(s) 563
Eco47I GGWCC 1 cut(s) 511
Eco57I CTGAAG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 53
EcoRV GATATC 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 777
ErhI CCWWGG 1 cut(s) 777
FaeI CATG 2 cut(s) 247, 476
FaiI YATR 9 cut(s) 149, 164, 170, 209, 235, 245, 461, 474, 766
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FatI CATG 2 cut(s) 243, 472
Fnu4HI GCNGC 2 cut(s) 434, 657
FokI GGATG 4 cut(s) 134, 296, 330, 697
Fsp4HI GCNGC 2 cut(s) 434, 657
FspBI CTAG 1 cut(s) 372
GluI GCNGC 2 cut(s) 434, 657
GsuI CTGGAG 2 cut(s) 587, 805
HaeIII GGCC 1 cut(s) 58
HapII CCGG 3 cut(s) 92, 739, 798
HgaI GACGC 1 cut(s) 653
Hin1II CATG 2 cut(s) 247, 476
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 1 cut(s) 391
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 3 cut(s) 92, 739, 798
HphI GGTGA 2 cut(s) 81, 563
Hpy166II GTNNAC 5 cut(s) 8, 131, 428, 454, 632
Hpy188I TCNGA 6 cut(s) 231, 410, 421, 504, 587, 619
Hpy188III TCNNGA 5 cut(s) 31, 189, 514, 566, 641
Hpy8I GTNNAC 5 cut(s) 8, 131, 428, 454, 632
HpyAV CCTTC 5 cut(s) 119, 511, 662, 675, 723
HpyCH4IV ACGT 2 cut(s) 444, 744
HpyCH4V TGCA 1 cut(s) 388
HpyF10VI GCNNNNNNNGC 3 cut(s) 394, 653, 761
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 2 cut(s) 444, 744
Hsp92II CATG 2 cut(s) 247, 476
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 2 cut(s) 191, 410
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 2 cut(s) 420, 643
LweI GCATC 1 cut(s) 356
MaeI CTAG 1 cut(s) 372
MaeII ACGT 2 cut(s) 444, 744
MalI GATC 2 cut(s) 193, 412
MboI GATC 2 cut(s) 191, 410
MboII GAAGA 2 cut(s) 19, 839
MfeI CAATTG 1 cut(s) 539
MluCI AATT 6 cut(s) 95, 351, 415, 479, 539, 734
MlyI GAGTC 1 cut(s) 385
MmeI TCCRAC 3 cut(s) 85, 261, 465
MnlI CCTC 9 cut(s) 27, 101, 258, 320, 541, 547, 604, 648, 775
MseI TTAA 5 cut(s) 453, 498, 528, 807, 850
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 3 cut(s) 92, 739, 798
MspR9I CCNGG 1 cut(s) 55
MunI CAATTG 1 cut(s) 539
MvaI CCWGG 1 cut(s) 55
MwoI GCNNNNNNNGC 3 cut(s) 394, 653, 761
NdeII GATC 2 cut(s) 191, 410
NlaIII CATG 2 cut(s) 247, 476
PaqCI CACCTGC 1 cut(s) 166
PkrI GCNGC 2 cut(s) 435, 658
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp1406I AACGTT 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 53
PspGI CCWGG 1 cut(s) 53
PspPI GGNCC 2 cut(s) 57, 511
PstNI CAGNNNCTG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SaqAI TTAA 5 cut(s) 453, 498, 528, 807, 850
SatI GCNGC 2 cut(s) 434, 657
Sau3AI GATC 2 cut(s) 191, 410
Sau96I GGNCC 2 cut(s) 57, 511
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 1 cut(s) 55
SfaNI GCATC 1 cut(s) 356
SinI GGWCC 1 cut(s) 511
SmlI CTYRAG 1 cut(s) 639
SmoI CTYRAG 1 cut(s) 639
Sse9I AATT 6 cut(s) 95, 351, 415, 479, 539, 734
SsiI CCGC 3 cut(s) 470, 545, 624
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 53
StyI CCWWGG 1 cut(s) 777
TaiI ACGT 2 cut(s) 447, 747
TaqI TCGA 6 cut(s) 30, 67, 312, 609, 666, 792
TasI AATT 6 cut(s) 95, 351, 415, 479, 539, 734
TatI WGTACW 1 cut(s) 5
Tru1I TTAA 5 cut(s) 453, 498, 528, 807, 850
Tru9I TTAA 5 cut(s) 453, 498, 528, 807, 850
TscAI CASTG 1 cut(s) 829
TseI GCWGC 2 cut(s) 433, 656
TspDTI ATGAA 3 cut(s) 138, 263, 369
TspRI CASTG 1 cut(s) 829
VpaK11BI GGWCC 1 cut(s) 511
XapI RAATTY 3 cut(s) 95, 415, 734
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.