AT4G37060

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005216
Physical Location & Seq
Reverse (-)
17461366 .. 17463961
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37060.2

Sequence Viewer

Length: 1245 bp
ATGGAGAACGAATCGCCCTCCAAGAAAAACATGCCGCCATCGTGCGGAACACTCGTAACCATCCTCAGCCTCGATGGTGGTGGAGTCAGAGGAATCATCGCCGGAGTAATCCTTGCCTATCTCGAAAAACAACTTCAGGAACTAGATGGAGAACACGTGAGGGTAGCTGATTATTTCGACGTGATAGCCGGAACTAGCACCGGTGGTCTTGTGACGGCGATGTTGACTGCACCGGACGAGAACGGTAGACCTCGTTTCGCGGCTAAAGAAATTGTGCCGTTTTACCTTGAACATTGTCCCAAGATATTTCCCCAGCCCACAGGCGTGCTTGCTCTGTTACCGAAGCTTCCTAAGCTTCTGTCTGGTCCAAAGTACAGCGGAAACTATCTGCGAACTACACTTGGTAAGCTTCTTGGAGAGACAAAACTTCGCCAGACACTCACAAACGTTGTTATACCTACCTTCGACATCAAGACACTTCAACCCACTATTTTCTCATCTTACCAGGCGTTGACTGATCCTAGCTTGGACGTCAAGGTATCGGACATATGCATCGGCACATCAGCTGCTCCCACTTACTTTCCTCCTTATTACTTTTCCAACGAAGACAGTCAAGGCAAGACTAGGCATTTTAACCTCGTTGATGGCGGGGTTACTGCTAATAACCCGACTTTGGTGGCCATGACTGCTGTGACGAAGCAGATTGTGAATAATAACCCTGATATGGGTACTCTCAATCCGTTAGGTTACGACCAGTTTCTCGTTATATCGATAGGAACAGGATCTGCAAAAAAAGAAGAGAGGTACAGCGCAAAGAAGGCTGCAAAATGGGGAATCATATCTTGGTTATATGAAGATGGAACTACTCCGATATTAGACATTACCTTTGAATCAAGCCGCGACATAGTCCATTATCACAGCTCTGTTGTGTTTAAAGCCCTACAATCTGAAGACAAGTACCTCAGAATCGATGATGATACATTGGAAGGAGATGCAAGCACTCTGGATCTATCGACAAAGTCTAATTTAGAAAATCTTATAAAACTTGGAGAGAAGATGCTGACAAACAGAGTCATGCAAATGAACATCGACACTGGTACATATGAACCTGCTGCTGAAAATATTAACAATGATGAACAACTCAAGAGGTTTGCGAAAATCCTCTCCGAGGAAAGGAAATTAAGGAGAAAGAGGAGCGACAAAATGACTAAAGATTCATCAATCGGATCACAAGAGATCAAGTAA

Protein Analysis

414

Amino Acids

45.64

Weight (kDa)

6.06

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 259 1e-24 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1040
AatII GACGTC 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 1117
AccI GTMKAC 1 cut(s) 247
AccII CGCG 2 cut(s) 260, 900
AciI CCGC 6 cut(s) 35, 45, 260, 378, 648, 898
AclI AACGTT 1 cut(s) 447
AclWI GGATC 4 cut(s) 512, 790, 1014, 1234
AcoI YGGCCR 1 cut(s) 678
AcuI CTGAAG 2 cut(s) 119, 969
AcvI CACGTG 1 cut(s) 157
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 5 cut(s) 374, 730, 806, 959, 1099
AfiI CCNNNNNNNGG 6 cut(s) 44, 673, 724, 725, 1168, 1173
AflIII ACRYGT 1 cut(s) 154
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 3 cut(s) 290, 482, 890
AjiI CACGTC 1 cut(s) 181
AjnI CCWGG 1 cut(s) 504
AjuI GAANNNNNNNTTGG 2 cut(s) 826, 858
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 7 cut(s) 167, 346, 355, 409, 525, 566, 921
AluI AGCT 7 cut(s) 167, 346, 355, 409, 525, 566, 921
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 4 cut(s) 512, 790, 1014, 1234
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 1 cut(s) 678
ApeKI GCWGC 3 cut(s) 566, 821, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 869, 901
AsiGI ACCGGT 1 cut(s) 200
AspLEI GCGC 1 cut(s) 812
AspS9I GGNCC 1 cut(s) 365
AvaII GGWCC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 680
BbrPI CACGTG 1 cut(s) 157
BbsI GAAGAC 2 cut(s) 612, 957
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 553, 808, 1099
BccI CCATC 6 cut(s) 46, 68, 68, 140, 638, 851
BceAI ACGGC 2 cut(s) 231, 262
BciT130I CCWGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 4 cut(s) 143, 195, 522, 624
BfuAI ACCTGC 1 cut(s) 1117
BisI GCNGC 6 cut(s) 35, 261, 567, 822, 898, 1113
BlsI GCNGC 6 cut(s) 36, 262, 568, 823, 899, 1114
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 365
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 3 cut(s) 561, 982, 1047
BpiI GAAGAC 2 cut(s) 612, 957
Bpu10I CCTNAGC 2 cut(s) 65, 351
BpuEI CTTGAG 1 cut(s) 1127
Bsa29I ATCGAT 2 cut(s) 770, 969
BsaAI YACGTR 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 1 cut(s) 1167
BsaWI WCCGGW 2 cut(s) 200, 232
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 6 cut(s) 44, 673, 724, 725, 1168, 1173
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 754, 1099
BseBI CCWGG 1 cut(s) 506
BseCI ATCGAT 2 cut(s) 770, 969
BseDI CCNNGG 1 cut(s) 1167
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 6 cut(s) 44, 673, 724, 725, 1168, 1173
BseMII CTCAG 2 cut(s) 79, 976
BseNI ACTGG 2 cut(s) 754, 1099
BseRI GAGGAG 1 cut(s) 1207
BseXI GCAGC 3 cut(s) 553, 808, 1099
BseYI CCCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 213
Bsh1236I CGCG 2 cut(s) 260, 900
BshFI GGCC 1 cut(s) 680
BshTI ACCGGT 1 cut(s) 200
BshVI ATCGAT 2 cut(s) 770, 969
BsiSI CCGG 4 cut(s) 102, 189, 201, 233
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 6 cut(s) 44, 673, 724, 725, 1168, 1173
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 680
Bsp143I GATC 5 cut(s) 517, 782, 1006, 1226, 1236
BspACI CCGC 6 cut(s) 35, 45, 260, 378, 648, 898
BspANI GGCC 1 cut(s) 680
BspCNI CTCAG 2 cut(s) 78, 975
BspDI ATCGAT 2 cut(s) 770, 969
BspFNI CGCG 2 cut(s) 260, 900
BspMI ACCTGC 1 cut(s) 1117
BspPI GGATC 4 cut(s) 512, 790, 1014, 1234
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 2 cut(s) 754, 1099
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 1167
BssMI GATC 5 cut(s) 517, 782, 1006, 1226, 1236
BssNI GRCGYC 1 cut(s) 531
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 2 cut(s) 245, 611
Bst6I CTCTTC 1 cut(s) 792
BstACI GRCGYC 1 cut(s) 531
BstBAI YACGTR 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 326, 330, 997
BstDEI CTNAG 3 cut(s) 65, 351, 962
BstENI CCTNNNNNAGG 1 cut(s) 1166
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 2 cut(s) 260, 900
BstHHI GCGC 1 cut(s) 812
BstKTI GATC 5 cut(s) 520, 785, 1009, 1229, 1239
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 5 cut(s) 517, 782, 1006, 1226, 1236
BstMWI GCNNNNNNNGC 3 cut(s) 352, 686, 818
BstNI CCWGG 1 cut(s) 506
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 504
BstUI CGCG 2 cut(s) 260, 900
BstV1I GCAGC 3 cut(s) 553, 808, 1099
BstV2I GAAGAC 2 cut(s) 612, 957
BstX2I RGATCY 2 cut(s) 782, 1006
BstYI RGATCY 2 cut(s) 782, 1006
Bsu15I ATCGAT 2 cut(s) 770, 969
BsuRI GGCC 1 cut(s) 680
BsuTUI ATCGAT 2 cut(s) 770, 969
BtgZI GCGATG 2 cut(s) 82, 233
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 1092
BveI ACCTGC 1 cut(s) 1117
Cac8I GCNNGC 3 cut(s) 326, 330, 997
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 1 cut(s) 812
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 365
ClaI ATCGAT 2 cut(s) 770, 969
Csp6I GTAC 5 cut(s) 373, 729, 805, 958, 1098
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 3 cut(s) 31, 682, 1075
CviQI GTAC 5 cut(s) 373, 729, 805, 958, 1098
DdeI CTNAG 3 cut(s) 65, 351, 962
DpnI GATC 5 cut(s) 519, 784, 1008, 1228, 1238
DpnII GATC 5 cut(s) 517, 782, 1006, 1226, 1236
DraI TTTAAA 1 cut(s) 934
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 119, 969
Eco72I CACGTG 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 1166
EcoRII CCWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 554
FaeI CATG 3 cut(s) 34, 685, 1078
FaqI GGGAC 1 cut(s) 282
FatI CATG 3 cut(s) 30, 681, 1074
FauI CCCGC 1 cut(s) 641
FauNDI CATATG 2 cut(s) 548, 1102
FblI GTMKAC 1 cut(s) 247
Fnu4HI GCNGC 6 cut(s) 35, 261, 567, 822, 898, 1113
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 6 cut(s) 35, 261, 567, 822, 898, 1113
FspBI CTAG 4 cut(s) 143, 195, 522, 624
GlaI GCGC 1 cut(s) 811
GluI GCNGC 6 cut(s) 35, 261, 567, 822, 898, 1113
GsaI CCCAGC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 680
HapII CCGG 4 cut(s) 102, 189, 201, 233
HhaI GCGC 1 cut(s) 812
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 3 cut(s) 34, 685, 1078
Hin6I GCGC 1 cut(s) 810
HinP1I GCGC 1 cut(s) 810
HincII GTYRAC 2 cut(s) 225, 513
HindII GTYRAC 2 cut(s) 225, 513
HindIII AAGCTT 3 cut(s) 344, 353, 407
HinfI GANTC 8 cut(s) 11, 84, 93, 834, 890, 966, 1071, 1214
HpaII CCGG 4 cut(s) 102, 189, 201, 233
Hpy166II GTNNAC 3 cut(s) 225, 248, 513
Hpy188I TCNGA 7 cut(s) 89, 544, 870, 949, 965, 1168, 1226
Hpy188III TCNNGA 5 cut(s) 122, 137, 472, 1004, 1144
Hpy8I GTNNAC 3 cut(s) 225, 248, 513
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 472, 811, 980
HpyCH4III ACNGT 2 cut(s) 245, 611
HpyCH4IV ACGT 4 cut(s) 156, 180, 447, 531
HpyCH4V TGCA 6 cut(s) 230, 552, 788, 824, 995, 1078
HpyF10VI GCNNNNNNNGC 3 cut(s) 352, 686, 818
HpyF3I CTNAG 3 cut(s) 65, 351, 962
HpySE526I ACGT 4 cut(s) 156, 180, 447, 531
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 3 cut(s) 34, 685, 1078
HspAI GCGC 1 cut(s) 810
Kzo9I GATC 5 cut(s) 517, 782, 1006, 1226, 1236
LmnI GCTCC 2 cut(s) 574, 1194
Lsp1109I GCAGC 3 cut(s) 553, 808, 1099
LweI GCATC 3 cut(s) 561, 982, 1047
MaeI CTAG 4 cut(s) 143, 195, 522, 624
MaeII ACGT 4 cut(s) 156, 180, 447, 531
MaeIII GTNAC 6 cut(s) 55, 211, 336, 652, 691, 746
MalI GATC 5 cut(s) 519, 784, 1008, 1228, 1238
MboI GATC 5 cut(s) 517, 782, 1006, 1226, 1236
MboII GAAGA 5 cut(s) 617, 809, 866, 962, 1066
MflI RGATCY 2 cut(s) 782, 1006
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 3 cut(s) 270, 1024, 1178
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 2 cut(s) 93, 1080
MmeI TCCRAC 1 cut(s) 624
Mox20I TGGCCA 1 cut(s) 680
Mph1103I ATGCAT 1 cut(s) 554
MscI TGGCCA 1 cut(s) 680
MseI TTAA 4 cut(s) 633, 933, 1125, 1181
MslI CAYNNNNRTG 2 cut(s) 323, 1079
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 2 cut(s) 378, 566
MspI CCGG 4 cut(s) 102, 189, 201, 233
MspR9I CCNGG 1 cut(s) 506
MvaI CCWGG 1 cut(s) 506
MvnI CGCG 2 cut(s) 260, 900
MwoI GCNNNNNNNGC 3 cut(s) 352, 686, 818
NdeI CATATG 2 cut(s) 548, 1102
NdeII GATC 5 cut(s) 517, 782, 1006, 1226, 1236
NlaIII CATG 3 cut(s) 34, 685, 1078
NmuCI GTSAC 2 cut(s) 211, 691
NsiI ATGCAT 1 cut(s) 554
NspI RCATGY 1 cut(s) 34
OliI CACNNNNGTG 1 cut(s) 323
PfeI GAWTC 6 cut(s) 11, 93, 834, 890, 966, 1214
PflFI GACNNNGTC 2 cut(s) 905, 1018
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 6 cut(s) 36, 262, 568, 823, 899, 1114
PleI GAGTC 2 cut(s) 92, 1079
PmaCI CACGTG 1 cut(s) 157
PmlI CACGTG 1 cut(s) 157
PpsI GAGTC 2 cut(s) 92, 1079
Ppu21I YACGTR 1 cut(s) 157
PsiI TTATAA 1 cut(s) 1040
Psp1406I AACGTT 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 504
PspCI CACGTG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 312
PspGI CCWGG 1 cut(s) 504
PspPI GGNCC 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 2 cut(s) 782, 1006
PsyI GACNNNGTC 2 cut(s) 905, 1018
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 5 cut(s) 374, 730, 806, 959, 1099
RsaNI GTAC 5 cut(s) 373, 729, 805, 958, 1098
RseI CAYNNNNRTG 2 cut(s) 323, 1079
SaqAI TTAA 4 cut(s) 633, 933, 1125, 1181
SatI GCNGC 6 cut(s) 35, 261, 567, 822, 898, 1113
Sau3AI GATC 5 cut(s) 517, 782, 1006, 1226, 1236
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 2 cut(s) 93, 1080
ScrFI CCNGG 1 cut(s) 506
SfaNI GCATC 3 cut(s) 561, 982, 1047
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 323, 1079
SmlI CTYRAG 1 cut(s) 1142
SmoI CTYRAG 1 cut(s) 1142
Sse9I AATT 3 cut(s) 270, 1024, 1178
SsiI CCGC 6 cut(s) 35, 45, 260, 378, 648, 898
SspI AATATT 1 cut(s) 1123
SspMI CTAG 4 cut(s) 143, 195, 522, 624
StyD4I CCNGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 245, 611
TaiI ACGT 4 cut(s) 159, 183, 450, 534
TaqI TCGA 8 cut(s) 72, 123, 177, 465, 770, 969, 1013, 1089
TasI AATT 3 cut(s) 270, 1024, 1178
TatI WGTACW 1 cut(s) 372
TauI GCSGC 3 cut(s) 37, 263, 900
TfiI GAWTC 6 cut(s) 11, 93, 834, 890, 966, 1214
Tru1I TTAA 4 cut(s) 633, 933, 1125, 1181
Tru9I TTAA 4 cut(s) 633, 933, 1125, 1181
TscAI CASTG 1 cut(s) 1099
TseFI GTSAC 2 cut(s) 211, 691
TseI GCWGC 3 cut(s) 566, 821, 1112
Tsp45I GTSAC 2 cut(s) 211, 691
TspDTI ATGAA 5 cut(s) 867, 1097, 1119, 1149, 1206
TspGWI ACGGA 1 cut(s) 729
TspRI CASTG 1 cut(s) 1099
Tth111I GACNNNGTC 2 cut(s) 905, 1018
VpaK11BI GGWCC 1 cut(s) 365
XagI CCTNNNNNAGG 1 cut(s) 1166
XceI RCATGY 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 247
XspI CTAG 4 cut(s) 143, 195, 522, 624
ZraI GACGTC 1 cut(s) 532
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.