AT4G37070

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005216
Physical Location & Seq
Reverse (-)
17464594 .. 17467186
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37070.2

Sequence Viewer

Length: 1152 bp
ATGGAGAACAAATCGCCCTCCAAGAAAAACAAACCGCCATCGTGCGGATCACTCGTAACCATCCTCAGCCTTGATGGTGGTGGAGTCAGAGGAATCATCGCCGGAGTAATCCTTGCCTTTCTCGAAAAACAACTTCAGGAACTCGATGGAGAAGAGGCGAGGCTTGCGGATTACTTCGACGTGATAGCTGGAACTAGCACCGGTGGTCTTGTGACGGCGATGTTGACTGTACCGGACGAGACCGGTCGACCTCATTTCGCGGCTAAAGACATTGTGCCGTTTTACCTTGAACATTGTCCCAAGATATTTCCCCAGCCCACAGGCGTGCTTGCTCTGTTACCGAAGCTTCCAAAGCTTCTGTCTGGTCCAAAGTACAGCGGAAAGTATCTGCGAAATCTTCTGAGTAAGCTTCTTGGAGAGACAAGACTTCACCAGACCCTCACAAACATTGTTATACCTACCTTCGATATCAAGAAACTTCAACCCACTATTTTCTCCTCTTACCAGCTGTTGGTTGACCCTAGCTTGGATGTCAAGGTATCAGACATATGCATCGGCACTTCAGCTGCTCCCACTTTCTTTCCTCCCCATTACTTTTCCAACGAAGACAGTCAAGGCAATAAGACGGAGTTTAATCTCGTTGATGGCGCGGTTACTGCTAATAACCCGACTTTGGTGGCCATGACAGCTGTGTCTAAGCAGATTGTGAAGAATAATCCTGATATGGGTAAGCTCAAGCCGTTAGGTTTCGACCGGTTTCTCGTTATATCGATAGGAACAGGATCAACAAAAAGGGAAGAGAAGTACAGCGCAAAAAAGGCTGCAAAATGGGGGATCATATCTTGGTTATATGACGATGGATCTACTCCGATATTAGACATTACCATGGAATCAAGCCGCGACATGATCCATTATCACAGCTCTGTTGTGTTTAAAGCCCTACAATCTGAAGACAAGTACCTCCGAATCGATGATGATACATTGGAAGGAGATGTAAGCACTATGGATCTAGCGACAAAGTCTAACTTGGAGAATCTTCAAAAGATTGGAGAGAAGATGCTGACAAACAGAGTCATGCAAATGAACATCGACACTGGTGTATATGAACCTGTTGCTGAAAATATTACCAATGATGAACAGCTAAAGAGGTGA

Protein Analysis

383

Amino Acids

42.13

Weight (kDa)

5.94

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 228 7.5e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 691
AccB7I CCANNNNNTGG 1 cut(s) 511
AccI GTMKAC 1 cut(s) 247
AccII CGCG 3 cut(s) 260, 650, 900
AciI CCGC 7 cut(s) 35, 45, 167, 260, 378, 650, 898
AclWI GGATC 6 cut(s) 55, 790, 842, 868, 901, 1014
AcoI YGGCCR 1 cut(s) 678
AcuI CTGAAG 3 cut(s) 119, 546, 969
AfaI GTAC 4 cut(s) 231, 374, 806, 959
AfiI CCNNNNNNNGG 5 cut(s) 44, 511, 526, 673, 725
AgeI ACCGGT 3 cut(s) 200, 242, 753
AgsI TTSAA 3 cut(s) 290, 482, 1040
AjiI CACGTC 1 cut(s) 181
AleI CACNNNNGTG 1 cut(s) 323
Alw26I GTCTC 2 cut(s) 233, 413
AlwI GGATC 6 cut(s) 55, 790, 842, 868, 901, 1014
AoxI GGCC 1 cut(s) 678
ApeKI GCWGC 2 cut(s) 566, 821
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AsiGI ACCGGT 3 cut(s) 200, 242, 753
AspLEI GCGC 2 cut(s) 650, 812
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 365
BalI TGGCCA 1 cut(s) 680
BbsI GAAGAC 2 cut(s) 612, 957
BbvCI CCTCAGC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 553, 808
BccI CCATC 6 cut(s) 46, 68, 68, 140, 638, 851
BceAI ACGGC 3 cut(s) 231, 262, 724
BcoDI GTCTC 2 cut(s) 233, 413
BfaI CTAG 3 cut(s) 195, 522, 1010
BisI GCNGC 4 cut(s) 261, 567, 822, 898
BlsI GCNGC 4 cut(s) 262, 568, 823, 899
Bme18I GGWCC 1 cut(s) 365
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 365
BmsI GCATC 2 cut(s) 561, 1047
BpiI GAAGAC 2 cut(s) 612, 957
Bpu10I CCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 719
Bsa29I ATCGAT 2 cut(s) 770, 969
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 885
BsaWI WCCGGW 4 cut(s) 200, 232, 242, 753
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 511, 526, 673, 725
Bse118I RCCGGY 3 cut(s) 200, 242, 753
Bse1I ACTGG 1 cut(s) 1099
BseCI ATCGAT 2 cut(s) 770, 969
BseDI CCNNGG 1 cut(s) 885
BseGI GGATG 2 cut(s) 60, 535
BseLI CCNNNNNNNGG 5 cut(s) 44, 511, 526, 673, 725
BseMII CTCAG 2 cut(s) 79, 392
BseNI ACTGG 1 cut(s) 1099
BseRI GAGGAG 1 cut(s) 487
BseXI GCAGC 2 cut(s) 553, 808
BseYI CCCAGC 1 cut(s) 312
Bsh1236I CGCG 3 cut(s) 260, 650, 900
Bsh1285I CGRYCG 2 cut(s) 247, 754
BshFI GGCC 1 cut(s) 680
BshTI ACCGGT 3 cut(s) 200, 242, 753
BshVI ATCGAT 2 cut(s) 770, 969
BsiEI CGRYCG 2 cut(s) 247, 754
BsiSI CCGG 5 cut(s) 102, 201, 233, 243, 754
BslFI GGGAC 1 cut(s) 282
BslI CCNNNNNNNGG 5 cut(s) 44, 511, 526, 673, 725
BsmAI GTCTC 2 cut(s) 233, 413
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 1 cut(s) 680
Bso31I GGTCTC 1 cut(s) 233
Bsp143I GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 7 cut(s) 35, 45, 167, 260, 378, 650, 898
BspANI GGCC 1 cut(s) 680
BspCNI CTCAG 2 cut(s) 78, 393
BspDI ATCGAT 2 cut(s) 770, 969
BspFNI CGCG 3 cut(s) 260, 650, 900
BspPI GGATC 6 cut(s) 55, 790, 842, 868, 901, 1014
BspTNI GGTCTC 1 cut(s) 233
BsrFI RCCGGY 3 cut(s) 200, 242, 753
BsrI ACTGG 1 cut(s) 1099
BssAI RCCGGY 3 cut(s) 200, 242, 753
BssECI CCNNGG 1 cut(s) 885
BssMI GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
BssT1I CCWWGG 1 cut(s) 885
Bst4CI ACNGT 2 cut(s) 229, 611
Bst6I CTCTTC 2 cut(s) 147, 792
BstC8I GCNNGC 3 cut(s) 165, 326, 330
BstDEI CTNAG 3 cut(s) 65, 401, 696
BstDSI CCRYGG 1 cut(s) 885
BstF5I GGATG 2 cut(s) 60, 535
BstFNI CGCG 3 cut(s) 260, 650, 900
BstHHI GCGC 2 cut(s) 650, 812
BstKTI GATC 6 cut(s) 50, 785, 837, 863, 909, 1009
BstMAI GTCTC 2 cut(s) 233, 413
BstMBI GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
BstMCI CGRYCG 2 cut(s) 247, 754
BstMWI GCNNNNNNNGC 5 cut(s) 164, 352, 656, 686, 818
BstUI CGCG 3 cut(s) 260, 650, 900
BstV1I GCAGC 2 cut(s) 553, 808
BstV2I GAAGAC 2 cut(s) 612, 957
BstX2I RGATCY 2 cut(s) 860, 1006
BstYI RGATCY 2 cut(s) 860, 1006
Bsu15I ATCGAT 2 cut(s) 770, 969
BsuRI GGCC 1 cut(s) 680
BsuTUI ATCGAT 2 cut(s) 770, 969
BtgI CCRYGG 1 cut(s) 885
BtgZI GCGATG 2 cut(s) 82, 233
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 2 cut(s) 60, 535
BtsIMutI CAGTG 1 cut(s) 1092
Cac8I GCNNGC 3 cut(s) 165, 326, 330
CfoI GCGC 2 cut(s) 650, 812
Cfr10I RCCGGY 3 cut(s) 200, 242, 753
Cfr13I GGNCC 1 cut(s) 365
ClaI ATCGAT 2 cut(s) 770, 969
Csp6I GTAC 4 cut(s) 230, 373, 805, 958
CspAI ACCGGT 3 cut(s) 200, 242, 753
CviAII CATG 4 cut(s) 682, 886, 904, 1075
CviQI GTAC 4 cut(s) 230, 373, 805, 958
DdeI CTNAG 3 cut(s) 65, 401, 696
DpnI GATC 6 cut(s) 49, 784, 836, 862, 908, 1008
DpnII GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
DraI TTTAAA 1 cut(s) 934
DrdI GACNNNNNNGTC 1 cut(s) 691
DseDI GACNNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 2 cut(s) 147, 792
EarI CTCTTC 2 cut(s) 147, 792
Eco130I CCWWGG 1 cut(s) 885
Eco31I GGTCTC 1 cut(s) 233
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 3 cut(s) 119, 546, 969
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 885
EcoT22I ATGCAT 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 4 cut(s) 685, 889, 907, 1078
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FaqI GGGAC 1 cut(s) 282
FatI CATG 4 cut(s) 681, 885, 903, 1074
FauNDI CATATG 1 cut(s) 548
FblI GTMKAC 1 cut(s) 247
Fnu4HI GCNGC 4 cut(s) 261, 567, 822, 898
FokI GGATG 2 cut(s) 47, 542
Fsp4HI GCNGC 4 cut(s) 261, 567, 822, 898
FspBI CTAG 3 cut(s) 195, 522, 1010
GlaI GCGC 2 cut(s) 649, 811
GluI GCNGC 4 cut(s) 261, 567, 822, 898
GsaI CCCAGC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 680
HapII CCGG 5 cut(s) 102, 201, 233, 243, 754
HhaI GCGC 2 cut(s) 650, 812
Hin1II CATG 4 cut(s) 685, 889, 907, 1078
Hin6I GCGC 2 cut(s) 648, 810
HinP1I GCGC 2 cut(s) 648, 810
HincII GTYRAC 3 cut(s) 225, 248, 517
HindII GTYRAC 3 cut(s) 225, 248, 517
HindIII AAGCTT 3 cut(s) 344, 353, 407
HinfI GANTC 6 cut(s) 84, 93, 890, 966, 1033, 1071
HpaII CCGG 5 cut(s) 102, 201, 233, 243, 754
HphI GGTGA 1 cut(s) 422
Hpy166II GTNNAC 3 cut(s) 225, 248, 517
Hpy188I TCNGA 6 cut(s) 89, 402, 544, 870, 949, 965
Hpy188III TCNNGA 4 cut(s) 122, 137, 472, 719
Hpy8I GTNNAC 3 cut(s) 225, 248, 517
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 472, 980
HpyCH4III ACNGT 2 cut(s) 229, 611
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 552, 824, 1078
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 352, 656, 686, 818
HpyF3I CTNAG 3 cut(s) 65, 401, 696
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 4 cut(s) 685, 889, 907, 1078
HspAI GCGC 2 cut(s) 648, 810
Kzo9I GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
LmnI GCTCC 1 cut(s) 574
Lsp1109I GCAGC 2 cut(s) 553, 808
LweI GCATC 2 cut(s) 561, 1047
MaeI CTAG 3 cut(s) 195, 522, 1010
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 4 cut(s) 55, 211, 336, 652
MalI GATC 6 cut(s) 49, 784, 836, 862, 908, 1008
MboI GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
MboII GAAGA 8 cut(s) 164, 389, 617, 721, 809, 962, 1028, 1066
MflI RGATCY 2 cut(s) 860, 1006
MlsI TGGCCA 1 cut(s) 680
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 2 cut(s) 93, 1080
MmeI TCCRAC 1 cut(s) 624
Mox20I TGGCCA 1 cut(s) 680
Mph1103I ATGCAT 1 cut(s) 554
MscI TGGCCA 1 cut(s) 680
MseI TTAA 2 cut(s) 633, 933
MslI CAYNNNNRTG 3 cut(s) 323, 884, 1079
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 4 cut(s) 378, 508, 566, 689
MspI CCGG 5 cut(s) 102, 201, 233, 243, 754
MvnI CGCG 3 cut(s) 260, 650, 900
MwoI GCNNNNNNNGC 5 cut(s) 164, 352, 656, 686, 818
NcoI CCATGG 1 cut(s) 885
NdeI CATATG 1 cut(s) 548
NdeII GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
NlaIII CATG 4 cut(s) 685, 889, 907, 1078
NmuCI GTSAC 1 cut(s) 211
NsiI ATGCAT 1 cut(s) 554
OliI CACNNNNGTG 1 cut(s) 323
PfeI GAWTC 4 cut(s) 93, 890, 966, 1033
PflFI GACNNNGTC 1 cut(s) 1018
PflMI CCANNNNNTGG 1 cut(s) 511
PinAI ACCGGT 3 cut(s) 200, 242, 753
PkrI GCNGC 4 cut(s) 262, 568, 823, 899
PleI GAGTC 2 cut(s) 92, 1079
PpsI GAGTC 2 cut(s) 92, 1079
PspFI CCCAGC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 365
PsuI RGATCY 2 cut(s) 860, 1006
PsyI GACNNNGTC 1 cut(s) 1018
PvuII CAGCTG 3 cut(s) 508, 566, 689
RsaI GTAC 4 cut(s) 231, 374, 806, 959
RsaNI GTAC 4 cut(s) 230, 373, 805, 958
RseI CAYNNNNRTG 3 cut(s) 323, 884, 1079
SalI GTCGAC 1 cut(s) 246
SaqAI TTAA 2 cut(s) 633, 933
SatI GCNGC 4 cut(s) 261, 567, 822, 898
Sau3AI GATC 6 cut(s) 47, 782, 834, 860, 906, 1006
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 2 cut(s) 93, 1080
SfaNI GCATC 2 cut(s) 561, 1047
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 3 cut(s) 323, 884, 1079
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
SsiI CCGC 7 cut(s) 35, 45, 167, 260, 378, 650, 898
SspI AATATT 1 cut(s) 1123
SspMI CTAG 3 cut(s) 195, 522, 1010
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 2 cut(s) 229, 611
TaiI ACGT 1 cut(s) 183
TaqI TCGA 9 cut(s) 123, 144, 177, 247, 465, 750, 770, 969, 1089
TatI WGTACW 2 cut(s) 372, 804
TauI GCSGC 2 cut(s) 263, 900
TfiI GAWTC 4 cut(s) 93, 890, 966, 1033
Tru1I TTAA 2 cut(s) 633, 933
Tru9I TTAA 2 cut(s) 633, 933
TscAI CASTG 1 cut(s) 1099
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 2 cut(s) 566, 821
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 3 cut(s) 1097, 1119, 1149
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 1 cut(s) 1099
Tth111I GACNNNGTC 1 cut(s) 1018
Van91I CCANNNNNTGG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 247
XspI CTAG 3 cut(s) 195, 522, 1010
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.