MD02G1169600.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005216
Physical Location & Seq
Forward (+)
14668454 .. 14671163
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1169600.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGAAAAAAAAAGCTCAAGCCCAAGACATGGAAACCTCATAACGATTCTCAGTATAGATGGGGGAGGAATTAGAGGGATCATCCCTGGTGCTCTTCTCGCTTACCTTGAATCTGAACTTCAGAAACTAGATGGTGAAGATGCAAGGCTTGCAGATTATTTTGACGTGATTTCTGGGACAAGTACAGGCGGTCTAATTGCGACCATGTTAGTAGCTCCAAACGAAAACAACAGACCATTATTCGCTGCAAAGGACATTGTCCCATTTTACCTTTCCAACTGCCCTAAAATTTTCCCACGATCAAGTGGACTTTGTGCCACAGTTAATGATCTAGTGAAAGCTCTAACAGGACCAAAGTATGATGGAAAGTATTTGCACAAGCTTGTAAGAGAGATAATAGGAGACAAAAGGTTGGACCAAACCTTGACGAACCTGGTTGTCCCAACTTTTGACATCAAGAAACTTCTGCCTGTCATATTTTCGTCTTATCAGGTGTCAAGCCGTCCAGTGCTGAATGCTAGACTTTCAGACATTTGCATTGGTACCTCAGCAGCACCAACTTACCTTCCTGCATACTATTTTGAGAACAAGGATCAACAAGGAGAATCCAAGGAGTTCAACCTCATCGATGGTGGCATAGCCGCGAATAATCCGTCATTGATTGCCATTAGTGAAGTGATAAAACAAATTACGAGCCAGAATCAAGATTACTTGGAGATTGAGCCAAAGGACTTGTATGATCGTTTCCTACTGATTTCACTGGGAACGGGTTCGGATAGGAGTGAACTCAAATACAATGCCAAGAAAGCTTCAAAATGGAATGTTATAAGCTGGTTGTACACTAATGGGTCAACTCCTTTGTTGGATTGCTTTGATGAAGCAACTTGCGATATGGTTGATTACCATAACTCTGCGGTTTTCCAAGCTCTCCGTTCTGAACAGAACTACCTCAGAATTGACGAGGACACACTAGAGGGAGATGTAGCTTCAGTTGATAAAGCTACACGGGAAAACTTGGAAAATCTAGTGGAAGTGGGAAACAAATTACTCAAGAAACCAGTTTGTCGCGTGAATTTGGATACTTGTCTCTATGAACCAGTTGAAGATGAGGGCACAAATGAAGAAGCACTTCAGAGGTTTGCAAAGTTACTGTCGGATGAAAAGAAGCTTCGGGAGTCAAAATCATCACAGTAA

Protein Analysis

398

Amino Acids

44.05

Weight (kDa)

5.04

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 17 - 222 2.2e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 827
Acc65I GGTACC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 29
AccII CGCG 2 cut(s) 644, 1068
AciI CCGC 3 cut(s) 189, 642, 914
AclWI GGATC 2 cut(s) 86, 600
AcsI RAATTY 2 cut(s) 288, 1072
AcuI CTGAAG 3 cut(s) 104, 972, 1115
AfaI GTAC 3 cut(s) 184, 544, 839
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 4 cut(s) 110, 619, 813, 1103
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 2 cut(s) 85, 432
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 2 cut(s) 396, 1091
AlwI GGATC 2 cut(s) 86, 600
ApeKI GCWGC 2 cut(s) 245, 551
ApoI RAATTY 2 cut(s) 288, 1072
ArsI GACNNNNNNTTYG 4 cut(s) 638, 670, 1136, 1168
Asp700I GAANNNNTTC 2 cut(s) 769, 1128
Asp718I GGTACC 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 350, 415
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 2 cut(s) 350, 415
BaeGI GKGCMC 1 cut(s) 1115
BanI GGYRCC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 94
BbvCI CCTCAGC 1 cut(s) 547
BbvI GCAGC 2 cut(s) 232, 563
BccI CCATC 4 cut(s) 53, 125, 356, 623
BceAI ACGGC 1 cut(s) 486
BciT130I CCWGG 2 cut(s) 87, 434
BciVI GTATCC 1 cut(s) 1072
BcoDI GTCTC 2 cut(s) 396, 1091
BfaI CTAG 5 cut(s) 128, 332, 519, 971, 1025
BfuI GTATCC 1 cut(s) 1072
BisI GCNGC 3 cut(s) 246, 552, 642
BlsI GCNGC 3 cut(s) 247, 553, 643
Bme1390I CCNGG 2 cut(s) 87, 434
Bme18I GGWCC 2 cut(s) 350, 415
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 350, 415
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 87, 434
BmrI ACTGGG 1 cut(s) 770
BmsI GCATC 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 770
Bpu10I CCTNAGC 1 cut(s) 547
BpuEI CTTGAG 1 cut(s) 1034
Bsa29I ATCGAT 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 85, 609
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 4 cut(s) 506, 765, 1058, 1097
BseBI CCWGG 2 cut(s) 87, 434
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 2 cut(s) 85, 609
BseGI GGATG 2 cut(s) 81, 1162
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMII CTCAG 3 cut(s) 64, 561, 964
BseNI ACTGG 4 cut(s) 506, 765, 1058, 1097
BseSI GKGCMC 1 cut(s) 1115
BseXI GCAGC 2 cut(s) 232, 563
Bsh1236I CGCG 2 cut(s) 644, 1068
BshNI GGYRCC 1 cut(s) 542
BshVI ATCGAT 1 cut(s) 627
BsiHKAI GWGCWC 1 cut(s) 94
BslFI GGGAC 3 cut(s) 190, 245, 425
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 2 cut(s) 396, 1091
BsmFI GGGAC 3 cut(s) 190, 245, 425
BsmI GAATGC 1 cut(s) 520
Bsp1286I GDGCHC 2 cut(s) 94, 1115
Bsp1407I TGTACA 1 cut(s) 837
Bsp143I GATC 5 cut(s) 78, 299, 328, 592, 739
BspACI CCGC 3 cut(s) 189, 642, 914
BspCNI CTCAG 3 cut(s) 63, 560, 963
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 2 cut(s) 644, 1068
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 2 cut(s) 86, 600
BspQI GCTCTTC 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 542
BsrGI TGTACA 1 cut(s) 837
BsrI ACTGG 4 cut(s) 506, 765, 1058, 1097
BssECI CCNNGG 2 cut(s) 85, 609
BssMI GATC 5 cut(s) 78, 299, 328, 592, 739
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 2 cut(s) 87, 434
Bst4CI ACNGT 3 cut(s) 322, 1152, 1191
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 837
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 3 cut(s) 50, 547, 950
BstF5I GGATG 2 cut(s) 81, 1162
BstFNI CGCG 2 cut(s) 644, 1068
BstKTI GATC 5 cut(s) 81, 302, 331, 595, 742
BstMAI GTCTC 2 cut(s) 396, 1091
BstMBI GATC 5 cut(s) 78, 299, 328, 592, 739
BstMWI GCNNNNNNNGC 3 cut(s) 98, 149, 806
BstNI CCWGG 2 cut(s) 87, 434
BstSCI CCNGG 2 cut(s) 85, 432
BstSLI GKGCMC 1 cut(s) 1115
BstUI CGCG 2 cut(s) 644, 1068
BstV1I GCAGC 2 cut(s) 232, 563
Bsu15I ATCGAT 1 cut(s) 627
BsuI GTATCC 1 cut(s) 1072
BsuTUI ATCGAT 1 cut(s) 627
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 2 cut(s) 81, 1162
BtsIMutI CAGTG 2 cut(s) 513, 758
Cac8I GCNNGC 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 350, 415
ClaI ATCGAT 1 cut(s) 627
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 3 cut(s) 183, 543, 838
CviAII CATG 2 cut(s) 29, 205
CviQI GTAC 3 cut(s) 183, 543, 838
DdeI CTNAG 3 cut(s) 50, 547, 950
DpnI GATC 5 cut(s) 80, 301, 330, 594, 741
DpnII GATC 5 cut(s) 78, 299, 328, 592, 739
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 350, 415
Eco57I CTGAAG 3 cut(s) 104, 972, 1115
EcoRII CCWGG 2 cut(s) 85, 432
EcoT14I CCWWGG 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 2 cut(s) 32, 208
FalI AAGNNNNNCTT 2 cut(s) 1113, 1145
FaqI GGGAC 3 cut(s) 190, 245, 425
FatI CATG 2 cut(s) 28, 204
Fnu4HI GCNGC 3 cut(s) 246, 552, 642
FokI GGATG 2 cut(s) 68, 1169
Fsp4HI GCNGC 3 cut(s) 246, 552, 642
FspBI CTAG 5 cut(s) 128, 332, 519, 971, 1025
GluI GCNGC 3 cut(s) 246, 552, 642
Hin1II CATG 2 cut(s) 32, 208
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HindIII AAGCTT 3 cut(s) 380, 807, 1166
HinfI GANTC 5 cut(s) 46, 110, 605, 700, 1175
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 4 cut(s) 308, 785, 840, 852
Hpy188I TCNGA 8 cut(s) 115, 123, 529, 775, 937, 953, 1134, 1156
Hpy188III TCNNGA 4 cut(s) 457, 704, 1051, 1172
Hpy8I GTNNAC 4 cut(s) 308, 785, 840, 852
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 3 cut(s) 322, 1152, 1191
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 7 cut(s) 143, 152, 248, 376, 537, 572, 1142
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 149, 806
HpyF3I CTNAG 3 cut(s) 50, 547, 950
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 2 cut(s) 32, 208
KpnI GGTACC 1 cut(s) 546
Kzo9I GATC 5 cut(s) 78, 299, 328, 592, 739
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 2 cut(s) 232, 563
LweI GCATC 1 cut(s) 130
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 5 cut(s) 128, 332, 519, 971, 1025
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 1146
MalI GATC 5 cut(s) 80, 301, 330, 594, 741
MboI GATC 5 cut(s) 78, 299, 328, 592, 739
MboII GAAGA 4 cut(s) 86, 149, 1115, 1133
MhlI GDGCHC 2 cut(s) 94, 1115
MluCI AATT 7 cut(s) 69, 195, 288, 687, 954, 1043, 1072
MlyI GAGTC 1 cut(s) 1184
MmeI TCCRAC 4 cut(s) 300, 393, 843, 1134
MroXI GAANNNNTTC 2 cut(s) 769, 1128
MseI TTAA 1 cut(s) 324
MspR9I CCNGG 2 cut(s) 87, 434
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 2 cut(s) 87, 434
MvnI CGCG 2 cut(s) 644, 1068
MwoI GCNNNNNNNGC 3 cut(s) 98, 149, 806
NdeII GATC 5 cut(s) 78, 299, 328, 592, 739
NlaIII CATG 2 cut(s) 32, 208
NlaIV GGNNCC 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 2 cut(s) 769, 1128
PfeI GAWTC 4 cut(s) 46, 110, 605, 700
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 29
PkrI GCNGC 3 cut(s) 247, 553, 643
PleI GAGTC 1 cut(s) 1183
PpsI GAGTC 1 cut(s) 1183
PsiI TTATAA 1 cut(s) 827
Psp6I CCWGG 2 cut(s) 85, 432
PspGI CCWGG 2 cut(s) 85, 432
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 2 cut(s) 350, 415
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 3 cut(s) 184, 544, 839
RsaNI GTAC 3 cut(s) 183, 543, 838
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 3 cut(s) 246, 552, 642
Sau3AI GATC 5 cut(s) 78, 299, 328, 592, 739
Sau96I GGNCC 2 cut(s) 350, 415
SchI GAGTC 1 cut(s) 1184
ScrFI CCNGG 2 cut(s) 87, 434
SduI GDGCHC 2 cut(s) 94, 1115
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 1 cut(s) 130
SinI GGWCC 2 cut(s) 350, 415
SmlI CTYRAG 2 cut(s) 16, 1049
SmoI CTYRAG 2 cut(s) 16, 1049
Sse9I AATT 7 cut(s) 69, 195, 288, 687, 954, 1043, 1072
SsiI CCGC 3 cut(s) 189, 642, 914
SspMI CTAG 5 cut(s) 128, 332, 519, 971, 1025
StyD4I CCNGG 2 cut(s) 85, 432
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 3 cut(s) 322, 1152, 1191
TaiI ACGT 1 cut(s) 168
TaqI TCGA 1 cut(s) 627
TasI AATT 7 cut(s) 69, 195, 288, 687, 954, 1043, 1072
TatI WGTACW 2 cut(s) 182, 837
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 4 cut(s) 46, 110, 605, 700
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 2 cut(s) 513, 765
TseI GCWGC 2 cut(s) 245, 551
TspDTI ATGAA 4 cut(s) 891, 1107, 1134, 1173
TspGWI ACGGA 2 cut(s) 642, 920
TspRI CASTG 2 cut(s) 513, 765
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 350, 415
XapI RAATTY 2 cut(s) 288, 1072
XmnI GAANNNNTTC 2 cut(s) 769, 1128
XspI CTAG 5 cut(s) 128, 332, 519, 971, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.