AT4G37445

BEST Arabidopsis thaliana protein match is Calcium-binding EF hand family protein (TAIR AT1G64850.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010188
Physical Location & Seq
Reverse (-)
17604094 .. 17604944
851 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37445.1

Sequence Viewer

Length: 486 bp
ATGGGTAGTTCAATGGGCTTTCTCGGAAAAGGGGTACCACCGACGCAGATGATGAATATGGTAATGGGTTCACTCTACAAACAATTTACCCAAAAAGCTATCAACAACTTCGACGATTTCCACGTTGCTGTTTTAGACATCTTTAACAACTTCAACTCGGCTTTACCAGGCAGGCACTTCGATTTCCCAACCCCGGACCAAATAAAGGTTTGTTTCGCGCGGTGGAAGGAAGCGAAAGACGAAGAAGAGAAGAAAAAATTGTTCATTGAGTTCATGTCAAAGAGCGTGAAGCCAAGTAAACTCGATGATGTCACTATGATCACCGGAATAGTATCTCCACCGGCGGCAATGGCGGCTAAGAGAGCAGGAGAGAATGTTCCTCAGTTAAAATTAATCAAACTTATACCTGATGTTATATTCGTCCCTACGGTCACCATTTTAGCCATTGTCTCTGCCAAACTCTCTAGACGAATGTATTTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.04

Weight (kDa)

9.58

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 22 - 150 2.9e-07 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 34
AccB1I GGYRCC 1 cut(s) 34
AccII CGCG 2 cut(s) 218, 220
AciI CCGC 3 cut(s) 220, 344, 353
AfaI GTAC 1 cut(s) 36
AfiI CCNNNNNNNGG 2 cut(s) 193, 205
AgsI TTSAA 3 cut(s) 12, 154, 481
AjnI CCWGG 1 cut(s) 166
AloI GAACNNNNNNTCC 2 cut(s) 360, 392
AluBI AGCT 1 cut(s) 98
AluI AGCT 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 454
AseI ATTAAT 1 cut(s) 392
Asp718I GGTACC 1 cut(s) 34
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 313, 424
AvaII GGWCC 1 cut(s) 196
BanI GGYRCC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 1 cut(s) 168
BclI TGATCA 1 cut(s) 318
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 1 cut(s) 465
BisI GCNGC 2 cut(s) 345, 354
BlsI GCNGC 2 cut(s) 346, 355
Bme1390I CCNGG 2 cut(s) 168, 194
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 2 cut(s) 168, 194
BpuMI CCSGG 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 205
Bse118I RCCGGY 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 354
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 193, 205
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 395
Bsh1236I CGCG 2 cut(s) 218, 220
BshNI GGYRCC 1 cut(s) 34
BsiSI CCGG 3 cut(s) 194, 324, 341
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 2 cut(s) 193, 205
BsmAI GTCTC 1 cut(s) 454
BsmFI GGGAC 1 cut(s) 407
Bsp143I GATC 1 cut(s) 318
BspACI CCGC 3 cut(s) 220, 344, 353
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 2 cut(s) 218, 220
BspLI GGNNCC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 340
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 357, 381
BstEII GGTNACC 1 cut(s) 430
BstFNI CGCG 2 cut(s) 218, 220
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 1 cut(s) 321
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 3 cut(s) 350, 353, 362
BstNI CCWGG 1 cut(s) 168
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 166, 192
BstUI CGCG 2 cut(s) 218, 220
Cac8I GCNNGC 1 cut(s) 173
CfoI GCGC 1 cut(s) 220
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 35
CviAII CATG 1 cut(s) 274
CviJI RGCY 6 cut(s) 18, 98, 161, 292, 356, 443
CviKI_1 RGCY 6 cut(s) 18, 98, 161, 292, 356, 443
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 2 cut(s) 357, 381
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 196
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 166
FaeI CATG 1 cut(s) 277
FaiI YATR 5 cut(s) 59, 275, 317, 404, 416
FaqI GGGAC 1 cut(s) 407
FatI CATG 1 cut(s) 273
FbaI TGATCA 1 cut(s) 318
Fnu4HI GCNGC 2 cut(s) 345, 354
Fsp4HI GCNGC 2 cut(s) 345, 354
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 219
GluI GCNGC 2 cut(s) 345, 354
HapII CCGG 3 cut(s) 194, 324, 341
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HpaII CCGG 3 cut(s) 194, 324, 341
HphI GGTGA 2 cut(s) 313, 424
Hpy166II GTNNAC 2 cut(s) 71, 299
Hpy188I TCNGA 1 cut(s) 26
Hpy188III TCNNGA 1 cut(s) 465
Hpy8I GTNNAC 2 cut(s) 71, 299
Hpy99I CGWCG 2 cut(s) 46, 116
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 353, 362
HpyF3I CTNAG 2 cut(s) 357, 381
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 1 cut(s) 218
KpnI GGTACC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 318
Kzo9I GATC 1 cut(s) 318
LpnPI CCDG 8 cut(s) 153, 157, 180, 207, 337, 351, 354, 420
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 310, 430
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 3 cut(s) 254, 257, 262
MluCI AATT 3 cut(s) 83, 257, 389
MnlI CCTC 1 cut(s) 390
MseI TTAA 3 cut(s) 144, 386, 392
MspI CCGG 3 cut(s) 194, 324, 341
MspR9I CCNGG 2 cut(s) 168, 194
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 2 cut(s) 218, 220
MwoI GCNNNNNNNGC 3 cut(s) 350, 353, 362
NciI CCSGG 1 cut(s) 194
NdeII GATC 1 cut(s) 318
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 1 cut(s) 137
NmuCI GTSAC 2 cut(s) 310, 430
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PkrI GCNGC 2 cut(s) 346, 355
PshBI ATTAAT 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 166
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 196
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
SaqAI TTAA 3 cut(s) 144, 386, 392
SatI GCNGC 2 cut(s) 345, 354
Sau3AI GATC 1 cut(s) 318
Sau96I GGNCC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 168, 194
SetI ASST 4 cut(s) 100, 126, 210, 409
SgrAI CRCCGGYG 1 cut(s) 340
SinI GGWCC 1 cut(s) 196
Sse9I AATT 3 cut(s) 83, 257, 389
SsiI CCGC 3 cut(s) 220, 344, 353
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 2 cut(s) 166, 192
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 111, 180, 303
TasI AATT 3 cut(s) 83, 257, 389
TauI GCSGC 2 cut(s) 347, 356
Tru1I TTAA 3 cut(s) 144, 386, 392
Tru9I TTAA 3 cut(s) 144, 386, 392
TseFI GTSAC 2 cut(s) 310, 430
Tsp45I GTSAC 2 cut(s) 310, 430
TspDTI ATGAA 3 cut(s) 68, 253, 262
VpaK11BI GGWCC 1 cut(s) 196
VspI ATTAAT 1 cut(s) 392
XbaI TCTAGA 1 cut(s) 464
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.