MD07G1105300.v1.1

BEST Arabidopsis thaliana protein match is Calcium-binding EF hand family protein (TAIR AT1G64850.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010188
Physical Location & Seq
Reverse (-)
11913552 .. 11915135
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1105300.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGGACTGTCTATGAGTTTCATGGGATTGGGAGGAGGGCTACCATCCGGCCGGATGCTAAACTTTTTAATGGCAACGCTGTACGATCAGTTCATCAAGAAAGATATCCAAAATTTTGAGGAATTCCAGTTCGCCATTCTCGATATTTTCAACACTTTTAATTCTTCTCTGCCCGGTAAACACTACGAAGTGCCGTTGCGCAAGGAAATCGAGGATATGTTCATACGTTGGGAAAATGCCAAAGTGCAAGACAGGAGGAACATCTTCATCCAATTTATGAAGGACAATGTAAGGCTAAGCAAGGTCGATAACGCTACCGTGATAACAGGAGTTGTAACGCCACCGGTAGCCATGGCTGCTAAAAGAGCAGGGGAGAGTGTGCCCCAGCTGAAAATGATCAAGGCCATTCCTGACGTCTTTTTCGTGCCATCAGCAACAGTGTTGGCTCTCATTTCTGTTAAGATTTCCAGAAGAATTTTCACGGCAAAAAAAGCATCTTCGACACACTCCGAACCGGATATCCAGATGAAGACCGAAACCATTGGCGAAGCCAGAGCACCCGAAACCATTGGCGAAGCCAGAGCACCGCAAGGAGGTGCGCCCACACCTTTGGTCATCGGAAATTCCCATGAGGAGCGAGAAATACGCCCCTCTAACCTGCACAGAACACCTTCGGTCATCGGAACTTCCCATGGGGAGCAAGAAATACGCCCCTCCAACCCGAACAGAAGGTGGTGTTTGACGAAATGCCAAAATGAAGACTGCATCTACTGCGCACGACCTTGTATCTTTGAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

29.79

Weight (kDa)

8.8

Isoelectric Point (pI)

55.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 24 - 151 8.4e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 417
Acc16I TGCGCA 2 cut(s) 200, 775
Acc36I ACCTGC 1 cut(s) 666
AciI CCGC 1 cut(s) 587
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 4 cut(s) 112, 122, 474, 622
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 593
AgeI ACCGGT 1 cut(s) 343
AgsI TTSAA 2 cut(s) 151, 794
AloI GAACNNNNNNTCC 4 cut(s) 113, 145, 504, 536
AluBI AGCT 1 cut(s) 388
AluI AGCT 1 cut(s) 388
Alw21I GWGCWC 2 cut(s) 559, 586
AoxI GGCC 2 cut(s) 49, 402
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 4 cut(s) 112, 122, 474, 622
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AsiGI ACCGGT 1 cut(s) 343
Asp700I GAANNNNTTC 2 cut(s) 263, 670
AspLEI GCGC 3 cut(s) 201, 601, 776
AsuC2I CCSGG 1 cut(s) 174
BaeGI GKGCMC 1 cut(s) 384
BbsI GAAGAC 2 cut(s) 536, 765
Bbv12I GWGCWC 2 cut(s) 559, 586
BbvI GCAGC 1 cut(s) 343
BccI CCATC 2 cut(s) 52, 436
BceAI ACGGC 2 cut(s) 178, 498
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 666
BisI GCNGC 1 cut(s) 357
BlpI GCTNAGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 45, 503, 774
BpiI GAAGAC 2 cut(s) 536, 765
Bpu1102I GCTNAGC 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 351, 691
BsaWI WCCGGW 2 cut(s) 343, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 351, 691
BseGI GGATG 3 cut(s) 44, 60, 267
BseLI CCNNNNNNNGG 1 cut(s) 593
BseNI ACTGG 1 cut(s) 127
BseRI GAGGAG 2 cut(s) 48, 647
BseSI GKGCMC 1 cut(s) 384
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 644
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 51, 404
BshTI ACCGGT 1 cut(s) 343
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 2 cut(s) 559, 586
BsiSI CCGG 5 cut(s) 48, 52, 174, 344, 515
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 1 cut(s) 593
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 2 cut(s) 51, 404
Bsp1286I GDGCHC 3 cut(s) 384, 559, 586
Bsp143I GATC 2 cut(s) 85, 396
Bsp1720I GCTNAGC 1 cut(s) 296
Bsp19I CCATGG 2 cut(s) 351, 691
BspACI CCGC 1 cut(s) 587
BspANI GGCC 2 cut(s) 51, 404
BspHI TCATGA 1 cut(s) 797
BspMI ACCTGC 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 351, 691
BssMI GATC 2 cut(s) 85, 396
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 351, 691
Bst4CI ACNGT 3 cut(s) 9, 319, 439
BstACI GRCGYC 1 cut(s) 414
BstAPI GCANNNNNTGC 1 cut(s) 771
BstDEI CTNAG 1 cut(s) 296
BstDSI CCRYGG 2 cut(s) 351, 691
BstF5I GGATG 3 cut(s) 44, 60, 267
BstHHI GCGC 3 cut(s) 201, 601, 776
BstKTI GATC 2 cut(s) 88, 399
BstMBI GATC 2 cut(s) 85, 396
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 4 cut(s) 356, 365, 491, 771
BstSCI CCNGG 1 cut(s) 172
BstSLI GKGCMC 1 cut(s) 384
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 2 cut(s) 536, 765
BstXI CCANNNNNNTGG 1 cut(s) 610
BstZI CGGCCG 1 cut(s) 49
BsuRI GGCC 2 cut(s) 51, 404
BtgI CCRYGG 2 cut(s) 351, 691
BtsCI GGATG 3 cut(s) 44, 60, 267
BtsIMutI CAGTG 1 cut(s) 444
BveI ACCTGC 1 cut(s) 666
CciI TCATGA 1 cut(s) 797
CfoI GCGC 3 cut(s) 201, 601, 776
Cfr10I RCCGGY 1 cut(s) 343
Csp6I GTAC 1 cut(s) 82
CspAI ACCGGT 1 cut(s) 343
CviAII CATG 5 cut(s) 22, 352, 629, 692, 798
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 1 cut(s) 296
DpnI GATC 2 cut(s) 87, 398
DpnII GATC 2 cut(s) 85, 396
EaeI YGGCCR 1 cut(s) 49
EagI CGGCCG 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 351, 691
Eco32I GATATC 2 cut(s) 106, 520
Eco52I CGGCCG 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 122
EcoRV GATATC 2 cut(s) 106, 520
EcoT14I CCWWGG 2 cut(s) 351, 691
ErhI CCWWGG 2 cut(s) 351, 691
FaeI CATG 5 cut(s) 25, 355, 632, 695, 801
FaiI YATR 9 cut(s) 14, 23, 218, 224, 278, 353, 630, 693, 799
FaqI GGGAC 1 cut(s) 18
FatI CATG 5 cut(s) 21, 351, 628, 691, 797
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 3 cut(s) 31, 67, 254
Fsp4HI GCNGC 1 cut(s) 357
FspI TGCGCA 2 cut(s) 200, 775
GlaI GCGC 3 cut(s) 200, 600, 775
GluI GCNGC 1 cut(s) 357
GsaI CCCAGC 1 cut(s) 388
HaeIII GGCC 2 cut(s) 51, 404
HapII CCGG 5 cut(s) 48, 52, 174, 344, 515
HhaI GCGC 3 cut(s) 201, 601, 776
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 5 cut(s) 25, 355, 632, 695, 801
Hin6I GCGC 3 cut(s) 199, 599, 774
HinP1I GCGC 3 cut(s) 199, 599, 774
HinfI GANTC 1 cut(s) 794
HpaII CCGG 5 cut(s) 48, 52, 174, 344, 515
Hpy166II GTNNAC 1 cut(s) 179
Hpy188I TCNGA 3 cut(s) 511, 620, 683
Hpy188III TCNNGA 6 cut(s) 97, 140, 410, 468, 523, 798
Hpy8I GTNNAC 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 274, 681, 723
HpyCH4III ACNGT 3 cut(s) 9, 319, 439
HpyCH4IV ACGT 2 cut(s) 226, 414
HpyCH4V TGCA 3 cut(s) 247, 661, 765
HpyF10VI GCNNNNNNNGC 4 cut(s) 356, 365, 491, 771
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 2 cut(s) 226, 414
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 5 cut(s) 25, 355, 632, 695, 801
HspAI GCGC 3 cut(s) 199, 599, 774
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 2 cut(s) 85, 396
LmnI GCTCC 2 cut(s) 634, 697
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 3 cut(s) 45, 503, 774
MaeII ACGT 2 cut(s) 226, 414
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 87, 398
MboI GATC 2 cut(s) 85, 396
MboII GAAGA 6 cut(s) 156, 256, 483, 489, 541, 770
MhlI GDGCHC 3 cut(s) 384, 559, 586
MluCI AATT 6 cut(s) 112, 122, 160, 272, 474, 622
MmeI TCCRAC 1 cut(s) 741
MnlI CCTC 9 cut(s) 26, 29, 112, 205, 249, 587, 625, 661, 724
MroXI GAANNNNTTC 2 cut(s) 263, 670
MseI TTAA 3 cut(s) 68, 159, 459
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 5 cut(s) 48, 52, 174, 344, 515
MspR9I CCNGG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 356, 365, 491, 771
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 2 cut(s) 351, 691
NdeII GATC 2 cut(s) 85, 396
NlaIII CATG 5 cut(s) 25, 355, 632, 695, 801
NsbI TGCGCA 2 cut(s) 200, 775
PagI TCATGA 1 cut(s) 797
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 420
PdmI GAANNNNTTC 2 cut(s) 263, 670
PfeI GAWTC 1 cut(s) 794
PinAI ACCGGT 1 cut(s) 343
PkrI GCNGC 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 384
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SaqAI TTAA 3 cut(s) 68, 159, 459
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 2 cut(s) 85, 396
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 3 cut(s) 384, 559, 586
SfaNI GCATC 3 cut(s) 45, 503, 774
Sse9I AATT 6 cut(s) 112, 122, 160, 272, 474, 622
SsiI CCGC 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 2 cut(s) 351, 691
TaaI ACNGT 3 cut(s) 9, 319, 439
TaiI ACGT 2 cut(s) 229, 417
TaqI TCGA 4 cut(s) 141, 210, 306, 500
TaqII GACCGA 2 cut(s) 548, 664
TasI AATT 6 cut(s) 112, 122, 160, 272, 474, 622
TfiI GAWTC 1 cut(s) 794
Tru1I TTAA 3 cut(s) 68, 159, 459
Tru9I TTAA 3 cut(s) 68, 159, 459
TscAI CASTG 1 cut(s) 444
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 7 cut(s) 10, 82, 211, 256, 293, 542, 771
TspRI CASTG 1 cut(s) 444
XapI RAATTY 4 cut(s) 112, 122, 474, 622
XmnI GAANNNNTTC 2 cut(s) 263, 670
ZraI GACGTC 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.