Rw1G017170

BEST Arabidopsis thaliana protein match is Calcium-binding EF hand family protein (TAIR AT1G64850.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010188
Physical Location & Seq
Reverse (-)
37997305 .. 37998222
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017170.1

Sequence Viewer

Length: 519 bp
ATGGGATTGTTAATGAGTTCCATGTCCATAGGTTATTTTTCTTTTCGTCTCCTTTTCTCATCTTATGGTGGGTCCGCAAAGATGATCAATTATGCAATGGAACCGGTGTATGACAAGTTTATACAGAATTCAGTTACAGATTTTGACGAGTTTCACGCTGCCATGCTTGGTGTTTGCAGCGCATTAAATGCTGCTATGCCCGGTAAACACTACAATGTGCCCGGACTGGAGGAAGTAAAGGAATTTCACAGTAAATGGAACGACTTATCGACTCAAGAAAAGAAGGAGCAATTCATCCAGTTTATGAAGGATAATGTGAGAGTTAACCAGGCTGATACCGCTACCTTGTTGACAGGAGTACTGGCGCCTCCAGGGGCCATGGCTGCTAAAAAAGCTGGGGAGAGTCTTCCCCAGCTGAAAATGATCAAGAACGTCCCGGACATCCTCTTCGTGCCGACGGCAACAGTAGTAGCACTCATTACTGTAAGGTTCTCCAAAAGACTTTTCACGCGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

19.09

Weight (kDa)

8.97

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 33 - 162 2.7e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccII CGCG 1 cut(s) 511
AciI CCGC 2 cut(s) 75, 339
AcsI RAATTY 2 cut(s) 127, 242
AcyI GRCGYC 1 cut(s) 365
AfaI GTAC 1 cut(s) 360
AgeI ACCGGT 1 cut(s) 103
AjnI CCWGG 2 cut(s) 327, 370
AjuI GAANNNNNNNTTGG 1 cut(s) 488
AluBI AGCT 3 cut(s) 395, 415, 516
AluI AGCT 3 cut(s) 395, 415, 516
Alw26I GTCTC 1 cut(s) 53
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 4 cut(s) 158, 177, 191, 383
ApoI RAATTY 2 cut(s) 127, 242
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
AsiGI ACCGGT 1 cut(s) 103
AspLEI GCGC 2 cut(s) 182, 367
AspS9I GGNCC 2 cut(s) 72, 375
AsuC2I CCSGG 3 cut(s) 201, 222, 437
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 4 cut(s) 145, 178, 189, 370
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 2 cut(s) 329, 372
BclI TGATCA 2 cut(s) 84, 423
BcnI CCSGG 3 cut(s) 201, 222, 437
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 1 cut(s) 517
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 4 cut(s) 159, 178, 192, 384
BlsI GCNGC 4 cut(s) 160, 179, 193, 385
BmcAI AGTACT 1 cut(s) 360
Bme1390I CCNGG 5 cut(s) 201, 222, 329, 372, 437
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 72, 375
BmiI GGNNCC 4 cut(s) 73, 102, 366, 376
BmrFI CCNGG 5 cut(s) 201, 222, 329, 372, 437
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 2 cut(s) 248, 354
BpuEI CTTGAG 1 cut(s) 258
BpuMI CCSGG 3 cut(s) 201, 222, 437
BsaHI GRCGYC 1 cut(s) 365
BsaJI CCNNGG 2 cut(s) 371, 378
BsaWI WCCGGW 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 231, 298, 366
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 2 cut(s) 329, 372
BseDI CCNNGG 2 cut(s) 371, 378
BseGI GGATG 2 cut(s) 294, 441
BseMI GCAATG 1 cut(s) 102
BseNI ACTGG 3 cut(s) 231, 298, 366
BseSI GKGCMC 1 cut(s) 222
BseXI GCAGC 4 cut(s) 145, 178, 189, 370
BseYI CCCAGC 2 cut(s) 395, 411
Bsh1236I CGCG 1 cut(s) 511
BshFI GGCC 1 cut(s) 377
BshNI GGYRCC 1 cut(s) 364
BshTI ACCGGT 1 cut(s) 103
BsiSI CCGG 4 cut(s) 104, 201, 222, 437
BslFI GGGAC 1 cut(s) 419
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 2 cut(s) 84, 423
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 2 cut(s) 75, 339
BspANI GGCC 1 cut(s) 377
BspFNI CGCG 1 cut(s) 511
BspLI GGNNCC 4 cut(s) 73, 102, 366, 376
BspT107I GGYRCC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 3 cut(s) 231, 298, 366
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 371, 378
BssMI GATC 2 cut(s) 84, 423
BssNI GRCGYC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 329, 372
Bst4CI ACNGT 3 cut(s) 251, 466, 484
Bst6I CTCTTC 1 cut(s) 452
BstACI GRCGYC 1 cut(s) 365
BstAPI GCANNNNNTGC 1 cut(s) 188
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 294, 441
BstFNI CGCG 1 cut(s) 511
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 2 cut(s) 182, 367
BstKTI GATC 2 cut(s) 87, 426
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 84, 423
BstMWI GCNNNNNNNGC 4 cut(s) 188, 338, 383, 392
BstNI CCWGG 2 cut(s) 329, 372
BstSCI CCNGG 5 cut(s) 199, 220, 327, 370, 435
BstSLI GKGCMC 1 cut(s) 222
BstUI CGCG 1 cut(s) 511
BstV1I GCAGC 4 cut(s) 145, 178, 189, 370
BstV2I GAAGAC 1 cut(s) 398
BsuRI GGCC 1 cut(s) 377
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 2 cut(s) 294, 441
CfoI GCGC 2 cut(s) 182, 367
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 72, 375
Csp6I GTAC 1 cut(s) 359
CspAI ACCGGT 1 cut(s) 103
CviAII CATG 3 cut(s) 22, 163, 379
CviJI RGCY 6 cut(s) 332, 377, 383, 395, 415, 516
CviKI_1 RGCY 6 cut(s) 332, 377, 383, 395, 415, 516
CviQI GTAC 1 cut(s) 359
DinI GGCGCC 1 cut(s) 366
DpnI GATC 2 cut(s) 86, 425
DpnII GATC 2 cut(s) 84, 423
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 72
EcoRI GAATTC 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 327, 370
EcoT14I CCWWGG 1 cut(s) 378
EgeI GGCGCC 1 cut(s) 366
EheI GGCGCC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 1 cut(s) 53
FaeI CATG 3 cut(s) 25, 166, 382
FaqI GGGAC 1 cut(s) 419
FatI CATG 3 cut(s) 21, 162, 378
FbaI TGATCA 2 cut(s) 84, 423
Fnu4HI GCNGC 4 cut(s) 159, 178, 192, 384
FokI GGATG 2 cut(s) 281, 428
Fsp4HI GCNGC 4 cut(s) 159, 178, 192, 384
FspBI CTAG 1 cut(s) 517
GlaI GCGC 2 cut(s) 181, 366
GluI GCNGC 4 cut(s) 159, 178, 192, 384
GsaI CCCAGC 2 cut(s) 399, 415
GsuI CTGGAG 2 cut(s) 248, 354
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 1 cut(s) 377
HapII CCGG 4 cut(s) 104, 201, 222, 437
HhaI GCGC 2 cut(s) 182, 367
Hin1I GRCGYC 1 cut(s) 365
Hin1II CATG 3 cut(s) 25, 166, 382
Hin6I GCGC 2 cut(s) 180, 365
HinP1I GCGC 2 cut(s) 180, 365
HincII GTYRAC 2 cut(s) 325, 351
HindII GTYRAC 2 cut(s) 325, 351
HinfI GANTC 2 cut(s) 271, 403
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 4 cut(s) 104, 201, 222, 437
Hpy166II GTNNAC 3 cut(s) 206, 325, 351
Hpy188III TCNNGA 2 cut(s) 275, 427
Hpy8I GTNNAC 3 cut(s) 206, 325, 351
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 2 cut(s) 277, 301
HpyCH4III ACNGT 3 cut(s) 251, 466, 484
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 2 cut(s) 95, 177
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 338, 383, 392
HpySE526I ACGT 1 cut(s) 432
Hsp92I GRCGYC 1 cut(s) 365
Hsp92II CATG 3 cut(s) 25, 166, 382
HspAI GCGC 2 cut(s) 180, 365
KasI GGCGCC 1 cut(s) 364
Ksp22I TGATCA 2 cut(s) 84, 423
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 84, 423
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 4 cut(s) 145, 178, 189, 370
MaeI CTAG 1 cut(s) 517
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 133
MalI GATC 2 cut(s) 86, 425
MboI GATC 2 cut(s) 84, 423
MboII GAAGA 2 cut(s) 398, 439
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 4 cut(s) 88, 127, 242, 290
Mly113I GGCGCC 1 cut(s) 365
MlyI GAGTC 2 cut(s) 265, 412
MnlI CCTC 3 cut(s) 223, 378, 455
MseI TTAA 3 cut(s) 11, 185, 324
MslI CAYNNNNRTG 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 4 cut(s) 104, 201, 222, 437
MspR9I CCNGG 5 cut(s) 201, 222, 329, 372, 437
MvaI CCWGG 2 cut(s) 329, 372
MvnI CGCG 1 cut(s) 511
MwoI GCNNNNNNNGC 4 cut(s) 188, 338, 383, 392
NarI GGCGCC 1 cut(s) 365
NciI CCSGG 3 cut(s) 201, 222, 437
NcoI CCATGG 1 cut(s) 378
NdeII GATC 2 cut(s) 84, 423
NlaIII CATG 3 cut(s) 25, 166, 382
NlaIV GGNNCC 4 cut(s) 73, 102, 366, 376
PfoI TCCNGGA 1 cut(s) 435
PinAI ACCGGT 1 cut(s) 103
PkrI GCNGC 4 cut(s) 160, 179, 193, 385
PleI GAGTC 2 cut(s) 265, 411
PluTI GGCGCC 1 cut(s) 368
PpsI GAGTC 2 cut(s) 265, 411
Psp6I CCWGG 2 cut(s) 327, 370
PspFI CCCAGC 2 cut(s) 395, 411
PspGI CCWGG 2 cut(s) 327, 370
PspN4I GGNNCC 4 cut(s) 73, 102, 366, 376
PspPI GGNCC 2 cut(s) 72, 375
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 3 cut(s) 11, 185, 324
SatI GCNGC 4 cut(s) 159, 178, 192, 384
Sau3AI GATC 2 cut(s) 84, 423
Sau96I GGNCC 2 cut(s) 72, 375
ScaI AGTACT 1 cut(s) 360
SchI GAGTC 2 cut(s) 265, 412
ScrFI CCNGG 5 cut(s) 201, 222, 329, 372, 437
SduI GDGCHC 1 cut(s) 222
SetI ASST 7 cut(s) 34, 347, 397, 417, 435, 491, 518
SfoI GGCGCC 1 cut(s) 366
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 4 cut(s) 88, 127, 242, 290
SsiI CCGC 2 cut(s) 75, 339
SspDI GGCGCC 1 cut(s) 364
SspMI CTAG 1 cut(s) 517
StyD4I CCNGG 5 cut(s) 199, 220, 327, 370, 435
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 3 cut(s) 251, 466, 484
TaiI ACGT 1 cut(s) 435
TaqI TCGA 1 cut(s) 269
TasI AATT 4 cut(s) 88, 127, 242, 290
TatI WGTACW 1 cut(s) 358
Tru1I TTAA 3 cut(s) 11, 185, 324
Tru9I TTAA 3 cut(s) 11, 185, 324
TseI GCWGC 4 cut(s) 158, 177, 191, 383
TspDTI ATGAA 2 cut(s) 283, 320
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 127, 242
XspI CTAG 1 cut(s) 517
ZrmI AGTACT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.