Rh1BG170400

BEST Arabidopsis thaliana protein match is Calcium-binding EF hand family protein (TAIR AT1G64850.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010188
Physical Location & Seq
Reverse (-)
27195859 .. 27196966
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG170400.1

Sequence Viewer

Length: 480 bp
ATGGGATTGTTAATGAGTTCCATGTCCCTAGGGTCCGCAAAGATGATCAATTGGGCAATGGAACCAGTGTATGACAAGTTTATACAGAATTCAGTTACAGAGTTTGACGAGTTTCACGCTGCCATGCTTGGTGTTTGCAGCGCATTAAATGCTGCTATGCCCGGTAAACACTACAATGTGCCCGGACTGGAGGAAGTAAAGGAATTTCACAGTAAATGGAACGGCTTATCGACTCAAGAAAAGAGGGAGCAATTCATCCAGTTTATGAAGGATAATGTGAGAGTTAACCAGGCTGATACCGCTACCTTGTTGACAGGAGTACTGGCGCCTCCAGGGGCCATGGCTGCTAAAAAAGCTGGGGAGAGTCTTCCCCAGCTGAAAATGATCAAGAACGTCCCGGACATCCTCTTCGTGCCGTCGGCAACAGTAGTAGCACTCATTACTGTAAGGTTCTCCAAAAGACTTTTCATGCGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.58

Weight (kDa)

9.07

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 20 - 148 3.8e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AciI CCGC 2 cut(s) 36, 300
AcsI RAATTY 2 cut(s) 88, 203
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 1 cut(s) 321
AjnI CCWGG 2 cut(s) 288, 331
AjuI GAANNNNNNNTTGG 1 cut(s) 449
AluBI AGCT 3 cut(s) 356, 376, 477
AluI AGCT 3 cut(s) 356, 376, 477
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 4 cut(s) 119, 138, 152, 344
ApoI RAATTY 2 cut(s) 88, 203
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspA2I CCTAGG 1 cut(s) 28
AspLEI GCGC 2 cut(s) 143, 328
AspS9I GGNCC 2 cut(s) 33, 336
AsuC2I CCSGG 3 cut(s) 162, 183, 398
AvaII GGWCC 1 cut(s) 33
AvrII CCTAGG 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 106, 139, 150, 331
BceAI ACGGC 2 cut(s) 238, 400
BciT130I CCWGG 2 cut(s) 290, 333
BclI TGATCA 2 cut(s) 45, 384
BcnI CCSGG 3 cut(s) 162, 183, 398
BfaI CTAG 2 cut(s) 29, 478
BfoI RGCGCY 1 cut(s) 329
BisI GCNGC 4 cut(s) 120, 139, 153, 345
BlnI CCTAGG 1 cut(s) 28
BlsI GCNGC 4 cut(s) 121, 140, 154, 346
BmcAI AGTACT 1 cut(s) 321
Bme1390I CCNGG 5 cut(s) 162, 183, 290, 333, 398
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 2 cut(s) 33, 336
BmiI GGNNCC 4 cut(s) 34, 63, 327, 337
BmrFI CCNGG 5 cut(s) 162, 183, 290, 333, 398
BpiI GAAGAC 1 cut(s) 359
BpmI CTGGAG 2 cut(s) 209, 315
BpuEI CTTGAG 1 cut(s) 219
BpuMI CCSGG 3 cut(s) 162, 183, 398
BsaHI GRCGYC 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 28, 332, 339
Bse1I ACTGG 4 cut(s) 65, 192, 259, 327
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 2 cut(s) 290, 333
BseDI CCNNGG 3 cut(s) 28, 332, 339
BseGI GGATG 2 cut(s) 255, 402
BseMI GCAATG 1 cut(s) 63
BseNI ACTGG 4 cut(s) 65, 192, 259, 327
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 4 cut(s) 106, 139, 150, 331
BseYI CCCAGC 2 cut(s) 356, 372
BshFI GGCC 1 cut(s) 338
BshNI GGYRCC 1 cut(s) 325
BsiSI CCGG 3 cut(s) 162, 183, 398
BslFI GGGAC 2 cut(s) 10, 380
BsmFI GGGAC 2 cut(s) 10, 380
BsnI GGCC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 45, 384
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 2 cut(s) 36, 300
BspANI GGCC 1 cut(s) 338
BspLI GGNNCC 4 cut(s) 34, 63, 327, 337
BspT107I GGYRCC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 63
BsrI ACTGG 4 cut(s) 65, 192, 259, 327
BssECI CCNNGG 3 cut(s) 28, 332, 339
BssMI GATC 2 cut(s) 45, 384
BssNI GRCGYC 1 cut(s) 326
BssT1I CCWWGG 2 cut(s) 28, 339
Bst2UI CCWGG 2 cut(s) 290, 333
Bst4CI ACNGT 3 cut(s) 212, 427, 445
Bst6I CTCTTC 1 cut(s) 413
BstACI GRCGYC 1 cut(s) 326
BstAPI GCANNNNNTGC 1 cut(s) 149
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 255, 402
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 2 cut(s) 143, 328
BstKTI GATC 2 cut(s) 48, 387
BstMBI GATC 2 cut(s) 45, 384
BstMWI GCNNNNNNNGC 4 cut(s) 149, 299, 344, 353
BstNI CCWGG 2 cut(s) 290, 333
BstSCI CCNGG 5 cut(s) 160, 181, 288, 331, 396
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 4 cut(s) 106, 139, 150, 331
BstV2I GAAGAC 1 cut(s) 359
BsuRI GGCC 1 cut(s) 338
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 255, 402
BtsIMutI CAGTG 1 cut(s) 72
CfoI GCGC 2 cut(s) 143, 328
Cfr13I GGNCC 2 cut(s) 33, 336
Csp6I GTAC 1 cut(s) 320
CviAII CATG 4 cut(s) 22, 124, 340, 469
CviJI RGCY 7 cut(s) 225, 293, 338, 344, 356, 376, 477
CviKI_1 RGCY 7 cut(s) 225, 293, 338, 344, 356, 376, 477
CviQI GTAC 1 cut(s) 320
DinI GGCGCC 1 cut(s) 327
DpnI GATC 2 cut(s) 47, 386
DpnII GATC 2 cut(s) 45, 384
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Eco130I CCWWGG 2 cut(s) 28, 339
Eco47I GGWCC 1 cut(s) 33
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 2 cut(s) 288, 331
EcoT14I CCWWGG 2 cut(s) 28, 339
EgeI GGCGCC 1 cut(s) 327
EheI GGCGCC 1 cut(s) 327
ErhI CCWWGG 2 cut(s) 28, 339
FaeI CATG 4 cut(s) 25, 127, 343, 472
FaiI YATR 8 cut(s) 23, 72, 83, 125, 158, 266, 341, 470
FaqI GGGAC 2 cut(s) 10, 380
FatI CATG 4 cut(s) 21, 123, 339, 468
FbaI TGATCA 2 cut(s) 45, 384
Fnu4HI GCNGC 4 cut(s) 120, 139, 153, 345
FokI GGATG 2 cut(s) 242, 389
Fsp4HI GCNGC 4 cut(s) 120, 139, 153, 345
FspBI CTAG 2 cut(s) 29, 478
GlaI GCGC 2 cut(s) 142, 327
GluI GCNGC 4 cut(s) 120, 139, 153, 345
GsaI CCCAGC 2 cut(s) 360, 376
GsuI CTGGAG 2 cut(s) 209, 315
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 1 cut(s) 338
HapII CCGG 3 cut(s) 162, 183, 398
HhaI GCGC 2 cut(s) 143, 328
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 4 cut(s) 25, 127, 343, 472
Hin6I GCGC 2 cut(s) 141, 326
HinP1I GCGC 2 cut(s) 141, 326
HincII GTYRAC 2 cut(s) 286, 312
HindII GTYRAC 2 cut(s) 286, 312
HinfI GANTC 2 cut(s) 232, 364
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 3 cut(s) 162, 183, 398
Hpy166II GTNNAC 3 cut(s) 167, 286, 312
Hpy188III TCNNGA 2 cut(s) 236, 388
Hpy8I GTNNAC 3 cut(s) 167, 286, 312
Hpy99I CGWCG 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 3 cut(s) 212, 427, 445
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 299, 344, 353
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 4 cut(s) 25, 127, 343, 472
HspAI GCGC 2 cut(s) 141, 326
KasI GGCGCC 1 cut(s) 325
Ksp22I TGATCA 2 cut(s) 45, 384
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 45, 384
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 4 cut(s) 106, 139, 150, 331
MaeI CTAG 2 cut(s) 29, 478
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 47, 386
MboI GATC 2 cut(s) 45, 384
MboII GAAGA 2 cut(s) 359, 400
MfeI CAATTG 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 4 cut(s) 49, 88, 203, 251
Mly113I GGCGCC 1 cut(s) 326
MlyI GAGTC 2 cut(s) 226, 373
MnlI CCTC 4 cut(s) 184, 237, 339, 416
MseI TTAA 3 cut(s) 11, 146, 285
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 3 cut(s) 162, 183, 398
MspR9I CCNGG 5 cut(s) 162, 183, 290, 333, 398
MunI CAATTG 1 cut(s) 49
MvaI CCWGG 2 cut(s) 290, 333
MwoI GCNNNNNNNGC 4 cut(s) 149, 299, 344, 353
NarI GGCGCC 1 cut(s) 326
NciI CCSGG 3 cut(s) 162, 183, 398
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 45, 384
NlaIII CATG 4 cut(s) 25, 127, 343, 472
NlaIV GGNNCC 4 cut(s) 34, 63, 327, 337
PfoI TCCNGGA 1 cut(s) 396
PkrI GCNGC 4 cut(s) 121, 140, 154, 346
PleI GAGTC 2 cut(s) 226, 372
PluTI GGCGCC 1 cut(s) 329
PpsI GAGTC 2 cut(s) 226, 372
Psp6I CCWGG 2 cut(s) 288, 331
PspFI CCCAGC 2 cut(s) 356, 372
PspGI CCWGG 2 cut(s) 288, 331
PspN4I GGNNCC 4 cut(s) 34, 63, 327, 337
PspPI GGNCC 2 cut(s) 33, 336
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 3 cut(s) 11, 146, 285
SatI GCNGC 4 cut(s) 120, 139, 153, 345
Sau3AI GATC 2 cut(s) 45, 384
Sau96I GGNCC 2 cut(s) 33, 336
ScaI AGTACT 1 cut(s) 321
SchI GAGTC 2 cut(s) 226, 373
ScrFI CCNGG 5 cut(s) 162, 183, 290, 333, 398
SduI GDGCHC 1 cut(s) 183
SetI ASST 6 cut(s) 308, 358, 378, 396, 452, 479
SfoI GGCGCC 1 cut(s) 327
SinI GGWCC 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 4 cut(s) 49, 88, 203, 251
SsiI CCGC 2 cut(s) 36, 300
SspDI GGCGCC 1 cut(s) 325
SspMI CTAG 2 cut(s) 29, 478
StyD4I CCNGG 5 cut(s) 160, 181, 288, 331, 396
StyI CCWWGG 2 cut(s) 28, 339
TaaI ACNGT 3 cut(s) 212, 427, 445
TaiI ACGT 1 cut(s) 396
TaqI TCGA 1 cut(s) 230
TasI AATT 4 cut(s) 49, 88, 203, 251
TatI WGTACW 1 cut(s) 319
Tru1I TTAA 3 cut(s) 11, 146, 285
Tru9I TTAA 3 cut(s) 11, 146, 285
TscAI CASTG 1 cut(s) 72
TseI GCWGC 4 cut(s) 119, 138, 152, 344
TspDTI ATGAA 3 cut(s) 244, 281, 457
TspRI CASTG 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 2 cut(s) 88, 203
XmaJI CCTAGG 1 cut(s) 28
XspI CTAG 2 cut(s) 29, 478
ZrmI AGTACT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.