MD07G1104600.v1.1

BEST Arabidopsis thaliana protein match is Calcium-binding EF hand family protein (TAIR AT1G64850.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010188
Physical Location & Seq
Reverse (-)
11851213 .. 11853077
1865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1104600.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGGACAGTCTATGAGTTTCATGGGATTGGGAGGAGGGCTGCGATCCAGCCAGATGCTAAACTTTTTAATGGGAAAGGTGTACCAACAGTTCATCGACAAAGATATCCAAAATTTTGAGGATTTCCAGTTGGCCATTCTCGATATTTTCAACACTTTCAATTCTTCTCTGCCCGGTAAACACTACAATGTGCCGTCGCACAAGGAAATTGAGGCTATGTTCATAAGTTGGAAAAATGCCAAAGTGCAAGACAGGAGGGACATCTTCATCCAATTTATGAAGGACAAAGTAAGGCTAAGCAAGGTCGATAACGCTACCGTGATAACAGGAGTTGTAACGCCGCCGGTAGCCATGGCTGCTAAAAGGGCAGGGGAGAGTGTGCCCCAGCTCAAAATGATCAAGGCCATTCCTGACGTCTTTTTCGTGCCATCAGCAACGGTATTGGCTCTCATTTCTGTTAAGATTTCCAGAAGAATTTTCACGGGAAAAAATGCATCTTCGACACACTCCGAACCTGATATCCAGACGAAGACCGAAACCATTGGCGTAGCTAGAGCACCCCAAGGAGGTGCTGCCGCATCTTCGGTCATCAGAAATCACCATGAGGAGCGAGAAATCCTTCCTGAAGGTCCCCCTGCAGCGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

23.63

Weight (kDa)

9.48

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7874 PF25284 24 - 151 1.3e-06 Family of unknown function (DUF7874)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 417
AciI CCGC 2 cut(s) 341, 576
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 2 cut(s) 112, 474
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 566
AgsI TTSAA 2 cut(s) 151, 160
AloI GAACNNNNNNTCC 2 cut(s) 504, 536
AluBI AGCT 2 cut(s) 388, 551
AluI AGCT 2 cut(s) 388, 551
Alw21I GWGCWC 1 cut(s) 559
AlwI GGATC 1 cut(s) 39
AoxI GGCC 2 cut(s) 132, 402
ApeKI GCWGC 4 cut(s) 40, 356, 572, 638
ApoI RAATTY 2 cut(s) 112, 474
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 1 cut(s) 629
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 590
AvaII GGWCC 1 cut(s) 629
BaeGI GKGCMC 1 cut(s) 384
BalI TGGCCA 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 3 cut(s) 27, 343, 559
BccI CCATC 1 cut(s) 436
BceAI ACGGC 1 cut(s) 178
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 1 cut(s) 174
BfaI CTAG 1 cut(s) 552
BfmI CTRYAG 1 cut(s) 636
BisI GCNGC 6 cut(s) 41, 341, 357, 573, 576, 639
BlpI GCTNAGC 1 cut(s) 296
BlsI GCNGC 6 cut(s) 42, 342, 358, 574, 577, 640
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 629
BmgT120I GGNCC 1 cut(s) 629
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 45, 503, 587
BpiI GAAGAC 1 cut(s) 536
Bpu1102I GCTNAGC 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 351, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 566
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 351, 562
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 566
BseNI ACTGG 1 cut(s) 127
BseRI GAGGAG 2 cut(s) 48, 620
BseSI GKGCMC 1 cut(s) 384
BseXI GCAGC 3 cut(s) 27, 343, 559
BseYI CCCAGC 1 cut(s) 384
BshFI GGCC 2 cut(s) 134, 404
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 2 cut(s) 174, 344
BslFI GGGAC 3 cut(s) 18, 272, 615
BslI CCNNNNNNNGG 1 cut(s) 566
BsmFI GGGAC 3 cut(s) 18, 272, 615
BsnI GGCC 2 cut(s) 134, 404
Bsp1286I GDGCHC 2 cut(s) 384, 559
Bsp143I GATC 2 cut(s) 44, 396
Bsp1720I GCTNAGC 1 cut(s) 296
Bsp19I CCATGG 1 cut(s) 351
BspACI CCGC 2 cut(s) 341, 576
BspANI GGCC 2 cut(s) 134, 404
BspLI GGNNCC 1 cut(s) 631
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 351, 562
BssMI GATC 2 cut(s) 44, 396
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 351, 562
Bst4CI ACNGT 4 cut(s) 9, 90, 319, 439
BstACI GRCGYC 1 cut(s) 414
BstDEI CTNAG 1 cut(s) 296
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 267
BstKTI GATC 2 cut(s) 47, 399
BstMBI GATC 2 cut(s) 44, 396
BstMWI GCNNNNNNNGC 2 cut(s) 356, 365
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 636
BstSLI GKGCMC 1 cut(s) 384
BstV1I GCAGC 3 cut(s) 27, 343, 559
BstV2I GAAGAC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 134, 404
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 629
CseI GACGC 1 cut(s) 630
Csp6I GTAC 1 cut(s) 82
CviAII CATG 3 cut(s) 22, 352, 602
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 1 cut(s) 296
DpnI GATC 2 cut(s) 46, 398
DpnII GATC 2 cut(s) 44, 396
EaeI YGGCCR 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 351, 562
Eco32I GATATC 2 cut(s) 106, 520
Eco47I GGWCC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 629
EcoRV GATATC 2 cut(s) 106, 520
EcoT14I CCWWGG 2 cut(s) 351, 562
EcoT22I ATGCAT 1 cut(s) 496
ErhI CCWWGG 2 cut(s) 351, 562
FaeI CATG 3 cut(s) 25, 355, 605
FaiI YATR 7 cut(s) 14, 23, 218, 224, 278, 353, 603
FaqI GGGAC 3 cut(s) 18, 272, 615
FatI CATG 3 cut(s) 21, 351, 601
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 6 cut(s) 41, 341, 357, 573, 576, 639
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 6 cut(s) 41, 341, 357, 573, 576, 639
FspBI CTAG 1 cut(s) 552
GluI GCNGC 6 cut(s) 41, 341, 357, 573, 576, 639
GsaI CCCAGC 1 cut(s) 388
HaeIII GGCC 2 cut(s) 134, 404
HapII CCGG 2 cut(s) 174, 344
HgaI GACGC 1 cut(s) 630
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 3 cut(s) 25, 355, 605
HpaII CCGG 2 cut(s) 174, 344
HphI GGTGA 1 cut(s) 590
Hpy166II GTNNAC 2 cut(s) 82, 179
Hpy188I TCNGA 2 cut(s) 511, 593
Hpy188III TCNNGA 5 cut(s) 140, 410, 468, 523, 623
Hpy8I GTNNAC 2 cut(s) 82, 179
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 3 cut(s) 274, 620, 629
HpyCH4III ACNGT 4 cut(s) 9, 90, 319, 439
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 3 cut(s) 247, 494, 638
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 365
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 3 cut(s) 25, 355, 605
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 2 cut(s) 44, 396
LmnI GCTCC 1 cut(s) 607
Lsp1109I GCAGC 3 cut(s) 27, 343, 559
LweI GCATC 3 cut(s) 45, 503, 587
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 46, 398
MboI GATC 2 cut(s) 44, 396
MboII GAAGA 6 cut(s) 156, 256, 483, 489, 541, 573
MhlI GDGCHC 2 cut(s) 384, 559
MlsI TGGCCA 1 cut(s) 134
MluCI AATT 5 cut(s) 112, 160, 207, 272, 474
MluNI TGGCCA 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 209
MnlI CCTC 7 cut(s) 26, 29, 112, 205, 249, 560, 598
Mox20I TGGCCA 1 cut(s) 134
Mph1103I ATGCAT 1 cut(s) 496
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 134
MseI TTAA 3 cut(s) 68, 459, 646
MslI CAYNNNNRTG 1 cut(s) 186
Msp20I TGGCCA 1 cut(s) 134
MspI CCGG 2 cut(s) 174, 344
MspR9I CCNGG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 356, 365
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 351
NdeII GATC 2 cut(s) 44, 396
NlaIII CATG 3 cut(s) 25, 355, 605
NlaIV GGNNCC 1 cut(s) 631
NsiI ATGCAT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PdmI GAANNNNTTC 1 cut(s) 618
PkrI GCNGC 6 cut(s) 42, 342, 358, 574, 577, 640
PpuMI RGGWCCY 1 cut(s) 629
Psp5II RGGWCCY 1 cut(s) 629
PspFI CCCAGC 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 631
PspPI GGNCC 1 cut(s) 629
PspPPI RGGWCCY 1 cut(s) 629
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PstI CTGCAG 1 cut(s) 640
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 3 cut(s) 68, 459, 646
SatI GCNGC 6 cut(s) 41, 341, 357, 573, 576, 639
Sau3AI GATC 2 cut(s) 44, 396
Sau96I GGNCC 1 cut(s) 629
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 2 cut(s) 384, 559
SetI ASST 8 cut(s) 81, 306, 390, 417, 517, 553, 571, 631
SfaNI GCATC 3 cut(s) 45, 503, 587
SfcI CTRYAG 1 cut(s) 636
SinI GGWCC 1 cut(s) 629
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 5 cut(s) 112, 160, 207, 272, 474
SsiI CCGC 2 cut(s) 341, 576
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 2 cut(s) 351, 562
TaaI ACNGT 4 cut(s) 9, 90, 319, 439
TaiI ACGT 1 cut(s) 417
TaqI TCGA 4 cut(s) 96, 141, 306, 500
TaqII GACCGA 2 cut(s) 548, 574
TasI AATT 5 cut(s) 112, 160, 207, 272, 474
TauI GCSGC 2 cut(s) 343, 578
Tru1I TTAA 3 cut(s) 68, 459, 646
Tru9I TTAA 3 cut(s) 68, 459, 646
TseI GCWGC 4 cut(s) 40, 356, 572, 638
TspDTI ATGAA 5 cut(s) 10, 82, 211, 256, 293
VpaK11BI GGWCC 1 cut(s) 629
XapI RAATTY 2 cut(s) 112, 474
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 1 cut(s) 552
ZraI GACGTC 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.