AT4G37925

Subunit NDH-M of NAD(P)H plastoquinone dehydrogenase complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016132
Physical Location & Seq
Reverse (-)
17830533 .. 17831619
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G37925.1

Sequence Viewer

Length: 654 bp
ATGGTTGCAGCATTCTCTTACACTGCCTGCACCAAGCTTTCTCTATTACATCCTTCTATGGTTGCTCAAATCCGACCAAGAACAACACAAAAGGCGTTCGTGGTGACAAATCCGGAACAAGACAGTACTCTTGAAGTACAAGAAACAGAGACACTCAAAGAAGAGCAATCAACAGAGAAGATGAAGAAGCAGCCAACACCATTGAGACCAGTGGAGAAACAGCTGAACGTGAAGAGCAAGGGTATGGGCGATTTTGGTGGTCAGTGGCTAAGCAGTGTCACACGTCACGTTAGGATCTACGCTGCTTATATTGATCCAGAGACTTGTGAGTTTGACCAGTCACAAATGGATAAACTCACTTTGATTCTTGATCCTACTGAAGAATTTGTGTGGGATGATGAAAGCTGTAACAAGGTCTATTCTTATTTCCAAGAACTCGTCGATCACTACGAGGGAGCACCATTAACGGAGTACACATTGAGATTGATAGGATCGGACGTAGAACATTACATAAGGAAGATGTTGTTCGATGGAGAAATACAATACAATATGGATGCAAGAGTTCTTAATTTCAGTATGGGAAAGCCTCGTGTACAATTCAATACCAGTAACATTGAAGGTGGTGGAGATGGTCAGCCTCAAGAGGACGCTTAG

Protein Analysis

217

Amino Acids

24.79

Weight (kDa)

4.81

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 90 - 199 6.1e-47 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 112
AclWI GGATC 4 cut(s) 302, 308, 365, 499
AcsI RAATTY 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 4 cut(s) 127, 138, 473, 594
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 3 cut(s) 134, 601, 617
AjiI CACGTC 1 cut(s) 284
AluBI AGCT 3 cut(s) 37, 223, 405
AluI AGCT 3 cut(s) 37, 223, 405
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 3 cut(s) 143, 199, 314
AlwI GGATC 4 cut(s) 302, 308, 365, 499
Aor13HI TCCGGA 1 cut(s) 112
ApeKI GCWGC 3 cut(s) 8, 190, 302
ApoI RAATTY 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 115
BauI CACGAG 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 3 cut(s) 20, 202, 289
BccI CCATC 2 cut(s) 524, 623
BcoDI GTCTC 3 cut(s) 143, 199, 314
BisI GCNGC 3 cut(s) 9, 191, 303
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 3 cut(s) 10, 192, 304
BmcAI AGTACT 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 284
BmsI GCATC 1 cut(s) 544
Bpu1102I GCTNAGC 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 199
BsaWI WCCGGW 1 cut(s) 112
Bse1I ACTGG 3 cut(s) 209, 337, 606
BseAI TCCGGA 1 cut(s) 112
BseGI GGATG 3 cut(s) 49, 400, 559
BseNI ACTGG 3 cut(s) 209, 337, 606
BseXI GCAGC 3 cut(s) 20, 202, 289
BsgI GTGCAG 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 460
BsiSI CCGG 1 cut(s) 113
BsmAI GTCTC 3 cut(s) 143, 199, 314
BsmI GAATGC 1 cut(s) 11
Bso31I GGTCTC 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 460
Bsp13I TCCGGA 1 cut(s) 112
Bsp1407I TGTACA 1 cut(s) 592
Bsp143I GATC 5 cut(s) 294, 313, 370, 442, 491
Bsp1720I GCTNAGC 1 cut(s) 269
BspEI TCCGGA 1 cut(s) 112
BspPI GGATC 4 cut(s) 302, 308, 365, 499
BspQI GCTCTTC 2 cut(s) 156, 227
BspTNI GGTCTC 1 cut(s) 199
BsrGI TGTACA 1 cut(s) 592
BsrI ACTGG 3 cut(s) 209, 337, 606
BssMI GATC 5 cut(s) 294, 313, 370, 442, 491
BssSI CACGAG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 588
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 2 cut(s) 156, 227
BstAUI TGTACA 1 cut(s) 592
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 269, 651
BstF5I GGATG 3 cut(s) 49, 400, 559
BstKTI GATC 5 cut(s) 297, 316, 373, 445, 494
BstMAI GTCTC 3 cut(s) 143, 199, 314
BstMBI GATC 5 cut(s) 294, 313, 370, 442, 491
BstV1I GCAGC 3 cut(s) 20, 202, 289
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 3 cut(s) 49, 400, 559
BtsI GCAGTG 2 cut(s) 21, 280
BtsIMutI CAGTG 4 cut(s) 21, 216, 269, 280
Cac8I GCNNGC 1 cut(s) 28
Csp6I GTAC 4 cut(s) 126, 137, 472, 593
CviJI RGCY 7 cut(s) 37, 193, 223, 268, 405, 586, 637
CviKI_1 RGCY 7 cut(s) 37, 193, 223, 268, 405, 586, 637
CviQI GTAC 4 cut(s) 126, 137, 472, 593
DdeI CTNAG 2 cut(s) 269, 651
DpnI GATC 5 cut(s) 296, 315, 372, 444, 493
DpnII GATC 5 cut(s) 294, 313, 370, 442, 491
Eam1104I CTCTTC 2 cut(s) 156, 227
EarI CTCTTC 2 cut(s) 156, 227
Eco31I GGTCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 399
FaiI YATR 6 cut(s) 59, 245, 309, 512, 551, 578
Fnu4HI GCNGC 3 cut(s) 9, 191, 303
FokI GGATG 3 cut(s) 36, 407, 566
Fsp4HI GCNGC 3 cut(s) 9, 191, 303
GluI GCNGC 3 cut(s) 9, 191, 303
HapII CCGG 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 1 cut(s) 364
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 474, 593
Hpy188I TCNGA 2 cut(s) 74, 496
Hpy188III TCNNGA 5 cut(s) 113, 131, 317, 368, 641
Hpy8I GTNNAC 2 cut(s) 474, 593
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 63, 611
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 4 cut(s) 228, 283, 288, 498
HpyCH4V TGCA 3 cut(s) 8, 30, 557
HpyF3I CTNAG 2 cut(s) 269, 651
HpySE526I ACGT 4 cut(s) 228, 283, 288, 498
Kpn2I TCCGGA 1 cut(s) 112
Kzo9I GATC 5 cut(s) 294, 313, 370, 442, 491
LguI GCTCTTC 2 cut(s) 156, 227
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 6 cut(s) 40, 126, 222, 330, 350, 619
Lsp1109I GCAGC 3 cut(s) 20, 202, 289
LweI GCATC 1 cut(s) 544
MaeII ACGT 4 cut(s) 228, 283, 288, 498
MaeIII GTNAC 6 cut(s) 103, 277, 284, 339, 407, 608
MalI GATC 5 cut(s) 296, 315, 372, 444, 493
MboI GATC 5 cut(s) 294, 313, 370, 442, 491
MboII GAAGA 6 cut(s) 173, 190, 196, 244, 392, 529
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 3 cut(s) 383, 568, 596
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 4 cut(s) 445, 597, 637, 648
MroI TCCGGA 1 cut(s) 112
MseI TTAA 2 cut(s) 464, 567
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 11
NdeII GATC 5 cut(s) 294, 313, 370, 442, 491
NmuCI GTSAC 4 cut(s) 103, 277, 284, 339
PciSI GCTCTTC 2 cut(s) 156, 227
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 3 cut(s) 10, 192, 304
PsuI RGATCY 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 4 cut(s) 127, 138, 473, 594
RsaNI GTAC 4 cut(s) 126, 137, 472, 593
SapI GCTCTTC 2 cut(s) 156, 227
SaqAI TTAA 2 cut(s) 464, 567
SatI GCNGC 3 cut(s) 9, 191, 303
Sau3AI GATC 5 cut(s) 294, 313, 370, 442, 491
ScaI AGTACT 1 cut(s) 127
SduI GDGCHC 1 cut(s) 460
SetI ASST 9 cut(s) 39, 225, 231, 286, 291, 407, 417, 501, 622
SfaNI GCATC 1 cut(s) 544
SmlI CTYRAG 1 cut(s) 639
SmoI CTYRAG 1 cut(s) 639
Sse9I AATT 3 cut(s) 383, 568, 596
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 4 cut(s) 231, 286, 291, 501
TaqI TCGA 2 cut(s) 441, 528
TasI AATT 3 cut(s) 383, 568, 596
TatI WGTACW 4 cut(s) 125, 136, 471, 592
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 464, 567
Tru9I TTAA 2 cut(s) 464, 567
TscAI CASTG 4 cut(s) 28, 216, 269, 280
TseFI GTSAC 4 cut(s) 103, 277, 284, 339
TseI GCWGC 3 cut(s) 8, 190, 302
Tsp45I GTSAC 4 cut(s) 103, 277, 284, 339
TspDTI ATGAA 2 cut(s) 197, 414
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 4 cut(s) 28, 216, 269, 280
XapI RAATTY 1 cut(s) 383
ZrmI AGTACT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.