Rh6BG385400

NAD(P)H-quinone oxidoreductase subunit M

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0016132
Physical Location & Seq
Forward (+)
61375069 .. 61376184
1116 bp
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UTR
Exon/CDS
Intron
Rh6BG385400.1

Sequence Viewer

Length: 609 bp
ATGGCAGCGACTTCTTCTTATATGGCTTGTGCAAAATTCTCTATGTTGGGTTATGCTGGAAGAAGAATCCAACTGAGAAGCAGAAGAGACTTCTTTATAAGAGCTCAGCAGCAACCTGGTGAAGTCCAAGAACCACAAGTACAGAGAGAAAAAGGAGAGCCAAAAGGAACAGTGCCAAGGCCAGTGGAGACACAATTGAATGTGAAGAGTAGGAACATGGCGAGAGAATATGGTGGGGATTGGCTTAGCTGTGCCACAAGGCATGTGAGGATTTATGCTGCTTATATAGACCCAGAAACCTGTGCATTTGATCAGACACAGATGGATAAGCTCACCATTATACTTGATCCAACCAATGAGTTTGTGTGGACTCCAGAGACTACCAACAAGGTCTACTCCTACTTCCAAGAGCTTGTGGATCACTATGAGGGTGCAGATTTGACAGAATACACTCTGCGTCTGATTGGTTCAGACATAGAGCACTACATAAGGAAGCTTCTCTATGACGGAGAAATCAAATACAACATGGATGCAAGAGTCCTTAACTTCAGCATGGGTAAGCCTCGCGTTATGTTCAATAACAATGAATTTCAACCTCAGGATGTATGA

Protein Analysis

202

Amino Acids

23.52

Weight (kDa)

5.97

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 82 - 190 1.8e-46 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AccI GTMKAC 1 cut(s) 393
AccII CGCG 1 cut(s) 567
AclWI GGATC 2 cut(s) 341, 426
AcsI RAATTY 2 cut(s) 35, 587
AcuI CTGAAG 1 cut(s) 532
AfaI GTAC 1 cut(s) 141
AgsI TTSAA 3 cut(s) 199, 577, 593
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 5 cut(s) 104, 249, 331, 412, 496
AluI AGCT 5 cut(s) 104, 249, 331, 412, 496
Alw21I GWGCWC 2 cut(s) 106, 483
Alw26I GTCTC 3 cut(s) 81, 182, 371
AlwI GGATC 2 cut(s) 341, 426
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 3 cut(s) 5, 109, 278
ApoI RAATTY 2 cut(s) 35, 587
AsuHPI GGTGA 2 cut(s) 131, 325
AxyI CCTNAGG 1 cut(s) 597
BanII GRGCYC 1 cut(s) 106
Bbv12I GWGCWC 2 cut(s) 106, 483
BbvI GCAGC 3 cut(s) 17, 121, 265
BccI CCATC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 81, 182, 371
BisI GCNGC 3 cut(s) 6, 110, 279
BlpI GCTNAGC 2 cut(s) 105, 245
BlsI GCNGC 3 cut(s) 7, 111, 280
Bme1390I CCNGG 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 520
BpmI CTGGAG 1 cut(s) 357
Bpu1102I GCTNAGC 2 cut(s) 105, 245
BsaJI CCNNGG 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 182
Bse21I CCTNAGG 1 cut(s) 597
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 176
BseGI GGATG 2 cut(s) 535, 607
BseMII CTCAG 2 cut(s) 65, 119
BseNI ACTGG 1 cut(s) 182
BseXI GCAGC 3 cut(s) 17, 121, 265
BsgI GTGCAG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 1 cut(s) 181
BsiHKAI GWGCWC 2 cut(s) 106, 483
BsmAI GTCTC 3 cut(s) 81, 182, 371
BsnI GGCC 1 cut(s) 181
Bsp1286I GDGCHC 2 cut(s) 106, 483
Bsp143I GATC 3 cut(s) 310, 346, 418
Bsp1720I GCTNAGC 2 cut(s) 105, 245
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 2 cut(s) 66, 118
BspFNI CGCG 1 cut(s) 567
BspPI GGATC 2 cut(s) 341, 426
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 3 cut(s) 310, 346, 418
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 79, 200
BstDEI CTNAG 4 cut(s) 74, 105, 245, 597
BstF5I GGATG 2 cut(s) 535, 607
BstFNI CGCG 1 cut(s) 567
BstKTI GATC 3 cut(s) 313, 349, 421
BstMAI GTCTC 3 cut(s) 81, 182, 371
BstMBI GATC 3 cut(s) 310, 346, 418
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 115
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 17, 121, 265
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 1 cut(s) 181
BtsCI GGATG 2 cut(s) 535, 607
BtsIMutI CAGTG 2 cut(s) 177, 189
CseI GACGC 1 cut(s) 446
CsiI ACCWGGT 1 cut(s) 115
Csp6I GTAC 1 cut(s) 140
CspCI CAANNNNNGTGG 2 cut(s) 165, 200
CviAII CATG 4 cut(s) 217, 263, 526, 553
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 4 cut(s) 74, 105, 245, 597
DpnI GATC 3 cut(s) 312, 348, 420
DpnII GATC 3 cut(s) 310, 346, 418
Eam1104I CTCTTC 2 cut(s) 79, 200
EarI CTCTTC 2 cut(s) 79, 200
Ecl136II GAGCTC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 532
Eco81I CCTNAGG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 4 cut(s) 220, 266, 529, 556
FatI CATG 4 cut(s) 216, 262, 525, 552
FbaI TGATCA 1 cut(s) 310
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 6, 110, 279
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 3 cut(s) 6, 110, 279
GluI GCNGC 3 cut(s) 6, 110, 279
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 181
HgaI GACGC 1 cut(s) 446
Hin1II CATG 4 cut(s) 220, 266, 529, 556
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 3 cut(s) 66, 370, 537
HphI GGTGA 2 cut(s) 131, 325
Hpy166II GTNNAC 2 cut(s) 369, 394
Hpy188I TCNGA 3 cut(s) 315, 462, 472
Hpy188III TCNNGA 2 cut(s) 374, 599
Hpy8I GTNNAC 2 cut(s) 369, 394
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 4 cut(s) 32, 305, 434, 533
HpyF3I CTNAG 4 cut(s) 74, 105, 245, 597
Hsp92II CATG 4 cut(s) 220, 266, 529, 556
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 3 cut(s) 310, 346, 418
LpnPI CCDG 8 cut(s) 42, 102, 129, 195, 306, 313, 387, 584
Lsp1109I GCAGC 3 cut(s) 17, 121, 265
LweI GCATC 1 cut(s) 520
MabI ACCWGGT 1 cut(s) 115
MalI GATC 3 cut(s) 312, 348, 420
MboI GATC 3 cut(s) 310, 346, 418
MboII GAAGA 5 cut(s) 6, 72, 75, 96, 217
MfeI CAATTG 1 cut(s) 194
MhlI GDGCHC 2 cut(s) 106, 483
MluCI AATT 3 cut(s) 35, 194, 587
MlyI GAGTC 2 cut(s) 364, 546
MmeI TCCRAC 2 cut(s) 94, 374
MnlI CCTC 4 cut(s) 261, 421, 573, 606
MseI TTAA 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 1 cut(s) 567
NdeII GATC 3 cut(s) 310, 346, 418
NlaIII CATG 4 cut(s) 220, 266, 529, 556
NspI RCATGY 1 cut(s) 266
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 3 cut(s) 7, 111, 280
PleI GAGTC 2 cut(s) 364, 545
PpsI GAGTC 2 cut(s) 364, 545
PsiI TTATAA 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PsrI GAACNNNNNNTAC 2 cut(s) 123, 155
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
SacI GAGCTC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 3 cut(s) 6, 110, 279
Sau3AI GATC 3 cut(s) 310, 346, 418
SchI GAGTC 2 cut(s) 364, 546
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 2 cut(s) 106, 483
SetI ASST 9 cut(s) 106, 118, 251, 302, 333, 393, 414, 498, 598
SexAI ACCWGGT 1 cut(s) 115
SfaNI GCATC 1 cut(s) 520
Sse9I AATT 3 cut(s) 35, 194, 587
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 1 cut(s) 172
TasI AATT 3 cut(s) 35, 194, 587
TatI WGTACW 1 cut(s) 139
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 2 cut(s) 177, 189
TseI GCWGC 3 cut(s) 5, 109, 278
TspDTI ATGAA 1 cut(s) 600
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 177, 189
XapI RAATTY 2 cut(s) 35, 587
XceI RCATGY 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.