Rh6DG377700

NAD(P)H-quinone oxidoreductase subunit M

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016132
Physical Location & Seq
Forward (+)
57213236 .. 57214385
1150 bp
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UTR
Exon/CDS
Intron
Rh6DG377700.1

Sequence Viewer

Length: 633 bp
ATGGCAGCGACTTCTTCTTATATGGCTTGTGCAAAATTCTCTATGTTGGGTTATGCTGGAAGAAGAATCCAACTGAGAAGCAGAAGAGACTTCTTTATAAGAGCTCAGCAGCAACCTGGTGAAGTCCAAGAACCACAAGAACTGAAAATACAAGAACCACAAGTACAGAGAGAAAAAGGAGAGCCAAAAGGAACAGTGCCAAGGCCAGTGGAGACACAATTGAATGTGAAGAGTAGGAACATGGCGAGAGAATATGGTGGGGATTGGCTTAGCTGTGCCACAAGGCATGTGAGGATTTATGCAGCTTATATAGACCCAGAAACCTGTGCATTTGATCAGACACAGATGGATAAGCTCACCATTATACTTGATCCAACCAATGAGTTTGTGTGGACTCCAGAGACTACCAACAAGGTCTACTCCTACTTCCAAGAGCTTGTGGATCACTATGAGGGTGCAGATTTGACAGAATACACTCTGCGTCTGATTGGTTCAGACATAGAGCACTACATAAGGAAGCTTCTCTATGACGGAGAAATCAAATACAACATGGATGCAAGAGTCCTTAACTTCAGCATGGGTAAGCCTCGCGTTATGTTCAATAACAACGAATTTCAACCTCAGGATGTGTGA

Protein Analysis

210

Amino Acids

24.49

Weight (kDa)

5.72

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 90 - 198 2e-46 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 98
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 591
AclWI GGATC 2 cut(s) 365, 450
AcsI RAATTY 2 cut(s) 35, 611
AcuI CTGAAG 1 cut(s) 556
AfaI GTAC 1 cut(s) 165
AgsI TTSAA 3 cut(s) 223, 601, 617
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 6 cut(s) 104, 273, 305, 355, 436, 520
AluI AGCT 6 cut(s) 104, 273, 305, 355, 436, 520
Alw21I GWGCWC 2 cut(s) 106, 507
Alw26I GTCTC 3 cut(s) 81, 206, 395
AlwI GGATC 2 cut(s) 365, 450
AoxI GGCC 1 cut(s) 203
ApeKI GCWGC 3 cut(s) 5, 109, 302
ApoI RAATTY 2 cut(s) 35, 611
AsuHPI GGTGA 2 cut(s) 131, 349
AxyI CCTNAGG 1 cut(s) 621
BanII GRGCYC 1 cut(s) 106
Bbv12I GWGCWC 2 cut(s) 106, 507
BbvI GCAGC 3 cut(s) 17, 121, 314
BccI CCATC 1 cut(s) 340
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 81, 206, 395
BisI GCNGC 3 cut(s) 6, 110, 303
BlpI GCTNAGC 2 cut(s) 105, 269
BlsI GCNGC 3 cut(s) 7, 111, 304
Bme1390I CCNGG 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 381
Bpu1102I GCTNAGC 2 cut(s) 105, 269
BsaJI CCNNGG 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 621
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 200
BseGI GGATG 2 cut(s) 559, 631
BseMII CTCAG 2 cut(s) 65, 119
BseNI ACTGG 1 cut(s) 206
BseXI GCAGC 3 cut(s) 17, 121, 314
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 1 cut(s) 591
BshFI GGCC 1 cut(s) 205
BsiHKAI GWGCWC 2 cut(s) 106, 507
BsmAI GTCTC 3 cut(s) 81, 206, 395
BsnI GGCC 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 106, 507
Bsp143I GATC 3 cut(s) 334, 370, 442
Bsp1720I GCTNAGC 2 cut(s) 105, 269
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 2 cut(s) 66, 118
BspFNI CGCG 1 cut(s) 591
BspPI GGATC 2 cut(s) 365, 450
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 3 cut(s) 334, 370, 442
BssT1I CCWWGG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 2 cut(s) 79, 224
BstDEI CTNAG 4 cut(s) 74, 105, 269, 621
BstF5I GGATG 2 cut(s) 559, 631
BstFNI CGCG 1 cut(s) 591
BstKTI GATC 3 cut(s) 337, 373, 445
BstMAI GTCTC 3 cut(s) 81, 206, 395
BstMBI GATC 3 cut(s) 334, 370, 442
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 115
BstUI CGCG 1 cut(s) 591
BstV1I GCAGC 3 cut(s) 17, 121, 314
Bsu36I CCTNAGG 1 cut(s) 621
BsuRI GGCC 1 cut(s) 205
BtsCI GGATG 2 cut(s) 559, 631
BtsIMutI CAGTG 2 cut(s) 201, 213
CseI GACGC 1 cut(s) 470
CsiI ACCWGGT 1 cut(s) 115
Csp6I GTAC 1 cut(s) 164
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 4 cut(s) 241, 287, 550, 577
CviQI GTAC 1 cut(s) 164
DdeI CTNAG 4 cut(s) 74, 105, 269, 621
DpnI GATC 3 cut(s) 336, 372, 444
DpnII GATC 3 cut(s) 334, 370, 442
Eam1104I CTCTTC 2 cut(s) 79, 224
EarI CTCTTC 2 cut(s) 79, 224
Ecl136II GAGCTC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 200
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 4 cut(s) 244, 290, 553, 580
FatI CATG 4 cut(s) 240, 286, 549, 576
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 6, 110, 303
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 3 cut(s) 6, 110, 303
GluI GCNGC 3 cut(s) 6, 110, 303
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 205
HgaI GACGC 1 cut(s) 470
Hin1II CATG 4 cut(s) 244, 290, 553, 580
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 3 cut(s) 66, 394, 561
HphI GGTGA 2 cut(s) 131, 349
Hpy166II GTNNAC 2 cut(s) 393, 418
Hpy188I TCNGA 3 cut(s) 339, 486, 496
Hpy188III TCNNGA 2 cut(s) 398, 623
Hpy8I GTNNAC 2 cut(s) 393, 418
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 5 cut(s) 32, 302, 329, 458, 557
HpyF3I CTNAG 4 cut(s) 74, 105, 269, 621
Hsp92II CATG 4 cut(s) 244, 290, 553, 580
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 334, 370, 442
LpnPI CCDG 8 cut(s) 42, 102, 129, 219, 330, 337, 411, 608
Lsp1109I GCAGC 3 cut(s) 17, 121, 314
LweI GCATC 1 cut(s) 544
MabI ACCWGGT 1 cut(s) 115
MalI GATC 3 cut(s) 336, 372, 444
MboI GATC 3 cut(s) 334, 370, 442
MboII GAAGA 5 cut(s) 6, 72, 75, 96, 241
MfeI CAATTG 1 cut(s) 218
MhlI GDGCHC 2 cut(s) 106, 507
MluCI AATT 3 cut(s) 35, 218, 611
MlyI GAGTC 2 cut(s) 388, 570
MmeI TCCRAC 2 cut(s) 94, 398
MnlI CCTC 4 cut(s) 285, 445, 597, 630
MseI TTAA 1 cut(s) 567
MspR9I CCNGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 218
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 1 cut(s) 591
NdeII GATC 3 cut(s) 334, 370, 442
NlaIII CATG 4 cut(s) 244, 290, 553, 580
NspI RCATGY 1 cut(s) 290
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 3 cut(s) 7, 111, 304
PleI GAGTC 2 cut(s) 388, 569
PpsI GAGTC 2 cut(s) 388, 569
PsiI TTATAA 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PsrI GAACNNNNNNTAC 4 cut(s) 132, 147, 164, 179
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
SacI GAGCTC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 3 cut(s) 6, 110, 303
Sau3AI GATC 3 cut(s) 334, 370, 442
SchI GAGTC 2 cut(s) 388, 570
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 2 cut(s) 106, 507
SexAI ACCWGGT 1 cut(s) 115
SfaNI GCATC 1 cut(s) 544
Sse9I AATT 3 cut(s) 35, 218, 611
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 196
TasI AATT 3 cut(s) 35, 218, 611
TatI WGTACW 1 cut(s) 163
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 2 cut(s) 201, 213
TseI GCWGC 3 cut(s) 5, 109, 302
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 2 cut(s) 201, 213
XapI RAATTY 2 cut(s) 35, 611
XceI RCATGY 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.