MD15G1133300.v1.1

NAD(P)H-quinone oxidoreductase subunit M

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016132
Physical Location & Seq
Reverse (-)
9663594 .. 9664802
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1133300.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCAGCAACAATGGCAAAGTTTCCACTCTTGGGCTATTTTGGAGGCAAAAAACAGCTCCAAAAAAGAAGGGATTTCTCCATAGCAGCTCAGCAGTCACAAGTCCAAGAGTCGCAAGAGAAAGCAGAGGAGGAGAGAGAGAGTGTGAGGACAGAGAAAGTGCTGGGAACGGGAGGCACACCACTGAGGCCGGTGGAGAAGCAGGTGAACGTGGAGAGCAAGAACATGGGGAGGGAGTACGGTGGGGATTGGCTGAGCAGTGCCACGAGGCACGTCAGGATCTACGCAGCTTACATTGACCCAGAGACATGTGCCTTCGACCAGACTCAGATGGATAAGCTTACTATTATTCTGGACCCCACCAATGAGTTTGTGTGGACTTCTGAGTCCTGCAACAAGGTCTACTCTTACTTCCAAGAGCTTGTGGATCACTATGAGGGAGCTCCATTGACAGAGTACACTCTCCGTCTGATCGGATCGGACATCGAGCACTACATAAGGAAGCTGCTATACGATGGGGAAATAAAGTACAACATGGATGCCAGAGTCCTCAATTTCAGCATGGGCAAGCCTCGCATGATGTTCAATAACAATGACATTCAACCTCAAGATGTACAACAGTCATAA

Protein Analysis

209

Amino Acids

23.96

Weight (kDa)

5.35

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 85 - 193 6.6e-46 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
AasI GACNNNNNNGTC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 193
AccI GTMKAC 1 cut(s) 402
AclWI GGATC 3 cut(s) 287, 435, 484
AfaI GTAC 4 cut(s) 239, 458, 530, 615
AfiI CCNNNNNNNGG 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 2 cut(s) 586, 602
AjiI CACGTC 1 cut(s) 274
AluBI AGCT 7 cut(s) 58, 89, 290, 340, 421, 443, 505
AluI AGCT 7 cut(s) 58, 89, 290, 340, 421, 443, 505
Alw21I GWGCWC 2 cut(s) 445, 492
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 3 cut(s) 287, 435, 484
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 4 cut(s) 5, 86, 287, 505
AspS9I GGNCC 1 cut(s) 355
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 445
BarI GAAGNNNNNNTAC 2 cut(s) 494, 526
BauI CACGAG 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 445, 492
BbvI GCAGC 4 cut(s) 17, 98, 299, 492
BccI CCATC 2 cut(s) 325, 509
BcoDI GTCTC 1 cut(s) 299
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 4 cut(s) 6, 87, 288, 506
BlpI GCTNAGC 2 cut(s) 90, 254
BlsI GCNGC 4 cut(s) 7, 88, 289, 507
Bme18I GGWCC 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 1 cut(s) 357
BmsI GCATC 1 cut(s) 529
Bpu1102I GCTNAGC 2 cut(s) 90, 254
BpuEI CTTGAG 1 cut(s) 591
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 190
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 5 cut(s) 104, 176, 245, 341, 375
BseRI GAGGAG 2 cut(s) 143, 146
BseXI GCAGC 4 cut(s) 17, 98, 299, 492
BseYI CCCAGC 1 cut(s) 163
BshFI GGCC 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 445, 492
BsiSI CCGG 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 299
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 445, 492
Bsp1407I TGTACA 1 cut(s) 613
Bsp143I GATC 4 cut(s) 279, 427, 471, 476
Bsp1720I GCTNAGC 2 cut(s) 90, 254
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 5 cut(s) 103, 177, 246, 340, 376
BspLI GGNNCC 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 3 cut(s) 287, 435, 484
BsrFI RCCGGY 1 cut(s) 190
BsrGI TGTACA 1 cut(s) 613
BssAI RCCGGY 1 cut(s) 190
BssMI GATC 4 cut(s) 279, 427, 471, 476
BssSI CACGAG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 265
Bst4CI ACNGT 2 cut(s) 242, 621
BstAUI TGTACA 1 cut(s) 613
BstC8I GCNNGC 1 cut(s) 569
BstDEI CTNAG 5 cut(s) 90, 185, 254, 327, 384
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 4 cut(s) 282, 430, 474, 479
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 4 cut(s) 279, 427, 471, 476
BstMWI GCNNNNNNNGC 2 cut(s) 14, 573
BstNSI RCATGY 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 17, 98, 299, 492
BstX2I RGATCY 1 cut(s) 279
BstYI RGATCY 1 cut(s) 279
BsuRI GGCC 1 cut(s) 190
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 2 cut(s) 182, 265
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 569
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 1 cut(s) 355
Csp6I GTAC 4 cut(s) 238, 457, 529, 614
CviAII CATG 5 cut(s) 226, 309, 535, 562, 577
CviQI GTAC 4 cut(s) 238, 457, 529, 614
DdeI CTNAG 5 cut(s) 90, 185, 254, 327, 384
DpnI GATC 4 cut(s) 281, 429, 473, 478
DpnII GATC 4 cut(s) 279, 427, 471, 476
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 355
Eco53kI GAGCTC 1 cut(s) 443
EcoICRI GAGCTC 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 445
FaeI CATG 5 cut(s) 229, 312, 538, 565, 580
FatI CATG 5 cut(s) 225, 308, 534, 561, 576
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 6, 87, 288, 506
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 4 cut(s) 6, 87, 288, 506
GluI GCNGC 4 cut(s) 6, 87, 288, 506
GsaI CCCAGC 1 cut(s) 167
HaeIII GGCC 1 cut(s) 190
HapII CCGG 1 cut(s) 191
Hin1II CATG 5 cut(s) 229, 312, 538, 565, 580
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 110, 325, 386, 546
HpaII CCGG 1 cut(s) 191
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 4 cut(s) 208, 378, 403, 459
Hpy188I TCNGA 5 cut(s) 330, 385, 471, 476, 481
Hpy188III TCNNGA 3 cut(s) 277, 353, 608
Hpy8I GTNNAC 4 cut(s) 208, 378, 403, 459
HpyAV CCTTC 2 cut(s) 63, 325
HpyCH4III ACNGT 2 cut(s) 242, 621
HpyCH4IV ACGT 2 cut(s) 210, 273
HpyCH4V TGCA 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 573
HpyF3I CTNAG 5 cut(s) 90, 185, 254, 327, 384
HpySE526I ACGT 2 cut(s) 210, 273
Hsp92II CATG 5 cut(s) 229, 312, 538, 565, 580
Kzo9I GATC 4 cut(s) 279, 427, 471, 476
LmnI GCTCC 3 cut(s) 63, 440, 448
LpnPI CCDG 9 cut(s) 149, 188, 204, 262, 315, 335, 338, 403, 556
Lsp1109I GCAGC 4 cut(s) 17, 98, 299, 492
LweI GCATC 1 cut(s) 529
MaeII ACGT 2 cut(s) 210, 273
MaeIII GTNAC 1 cut(s) 96
MalI GATC 4 cut(s) 281, 429, 473, 478
MboI GATC 4 cut(s) 279, 427, 471, 476
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 445, 492
MluCI AATT 1 cut(s) 553
MlyI GAGTC 4 cut(s) 119, 319, 395, 555
MspI CCGG 1 cut(s) 191
MwoI GCNNNNNNNGC 2 cut(s) 14, 573
NdeII GATC 4 cut(s) 279, 427, 471, 476
NlaIII CATG 5 cut(s) 229, 312, 538, 565, 580
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 96
NspI RCATGY 1 cut(s) 312
PaqCI CACCTGC 1 cut(s) 193
PciI ACATGT 1 cut(s) 308
PkrI GCNGC 4 cut(s) 7, 88, 289, 507
PleI GAGTC 4 cut(s) 118, 319, 394, 554
PpsI GAGTC 4 cut(s) 118, 319, 394, 554
PscI ACATGT 1 cut(s) 308
Psp124BI GAGCTC 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 1 cut(s) 355
PsuI RGATCY 1 cut(s) 279
RsaI GTAC 4 cut(s) 239, 458, 530, 615
RsaNI GTAC 4 cut(s) 238, 457, 529, 614
SacI GAGCTC 1 cut(s) 445
SatI GCNGC 4 cut(s) 6, 87, 288, 506
Sau3AI GATC 4 cut(s) 279, 427, 471, 476
Sau96I GGNCC 1 cut(s) 355
SchI GAGTC 4 cut(s) 119, 319, 395, 555
SduI GDGCHC 2 cut(s) 445, 492
SfaNI GCATC 1 cut(s) 529
SinI GGWCC 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
Sse9I AATT 1 cut(s) 553
SstI GAGCTC 1 cut(s) 445
TaaI ACNGT 2 cut(s) 242, 621
TaiI ACGT 2 cut(s) 213, 276
TaqI TCGA 2 cut(s) 318, 486
TasI AATT 1 cut(s) 553
TatI WGTACW 3 cut(s) 456, 528, 613
TscAI CASTG 2 cut(s) 189, 265
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 4 cut(s) 5, 86, 287, 505
Tsp45I GTSAC 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 2 cut(s) 189, 265
VpaK11BI GGWCC 1 cut(s) 355
XceI RCATGY 1 cut(s) 312
XmiI GTMKAC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.