pycom15g12020

NAD(P)H-quinone oxidoreductase subunit M

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016132
Physical Location & Seq
Reverse (-)
8161509 .. 8162602
1094 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12020.1

Sequence Viewer

Length: 615 bp
ATGGCAGCAACAATGGCAAAGTTTCCACTCTTGGGCTATTTTGGAGGCAAAAAAAAGCTCCAAAAGAGAAGGGATTTCTCCGTAGCAGCTCAGCAGCCAGAAGTCCAAGAGTCGCAGGAGGAGAGAGAAAGTGTGAGGACAGAGAAAGTGCTGGGAACGGGAGGCACACCACTGAGGCCGGTGGAGAAGCAGGTGAATGTGGAGAGCAAGAACATGGGGAGGGAGTACGGTGGGGATTGGCTGAGCAGTGCCACGAGGCACGTCAGGATCTACGCAGCTTACATTGACCCAGAGACTTGCGCCTTCGACCAGACTCAGATGGACAAGCTTACTATTATTCTGGACCCCACCAATGAGTTTGTGTGGACTTCTGAGTCCTGCAACAAGGTCTACTCTTACTTCCAAGAGCTTGTGGATCACTACGAGGGAGCTCCATTGACAGAGTACACTCTCCGTCTGATCGGTTCGGACATCGAGCACTACATAAGGAAGCTGCTATACGATGGGGAAATAAAGTACAACATGGATGCCAGAGTCCTCAACTTCAGCATGGGCAAGCCTCGCATGATGTTCAATAACAATGACATTCAACCGCAAGATGTACAACAGTCATAA

Protein Analysis

205

Amino Acids

23.5

Weight (kDa)

5.51

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NdhM PF10664 81 - 189 6.3e-46 Cyanobacterial and plastid NDH-1 subunit M
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37925
fragaria_vesca FvH4_2g27960
malus_domestica MD15G1133300.v1.1
prunus_persica Prupe.1G488900_v2.0.a1
pyrus_communis pycom15g12020
rosa_chinensis RchiOBHm_Chr6g0296781
rosa_laevigata RLG00000011659
rosa_multiflora Rmu_ssc0000192.1_g000012
rosa_roxburghii Rroxscaffold_7G00171170
rosa_rugosa Rorug06G0266100 Rorug06G0266200
rosa_samantha Rh6AG377200 Rh6BG385400 Rh6DG377700
rosa_wichuraiana Rw6G032960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 181
AasI GACNNNNNNGTC 1 cut(s) 373
Acc36I ACCTGC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 390
AciI CCGC 1 cut(s) 593
AclWI GGATC 2 cut(s) 275, 423
AcuI CTGAAG 1 cut(s) 529
AfaI GTAC 4 cut(s) 227, 446, 518, 603
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 2 cut(s) 574, 590
AjiI CACGTC 1 cut(s) 262
AluBI AGCT 7 cut(s) 58, 89, 278, 328, 409, 431, 493
AluI AGCT 7 cut(s) 58, 89, 278, 328, 409, 431, 493
Alw21I GWGCWC 2 cut(s) 433, 480
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 2 cut(s) 275, 423
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 5 cut(s) 5, 86, 94, 275, 493
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 205
AvaII GGWCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 433
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BauI CACGAG 1 cut(s) 253
Bbv12I GWGCWC 2 cut(s) 433, 480
BbvI GCAGC 5 cut(s) 17, 98, 106, 287, 480
BccI CCATC 2 cut(s) 313, 497
BcoDI GTCTC 1 cut(s) 287
BfuAI ACCTGC 1 cut(s) 181
BisI GCNGC 5 cut(s) 6, 87, 95, 276, 494
BlpI GCTNAGC 2 cut(s) 90, 242
BlsI GCNGC 5 cut(s) 7, 88, 96, 277, 495
Bme18I GGWCC 1 cut(s) 343
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 345
BmsI GCATC 1 cut(s) 517
Bpu1102I GCTNAGC 2 cut(s) 90, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 178
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 5 cut(s) 104, 164, 233, 329, 363
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 5 cut(s) 17, 98, 106, 287, 480
BseYI CCCAGC 1 cut(s) 151
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 2 cut(s) 433, 480
BsiSI CCGG 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 287
BsnI GGCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 433, 480
Bsp1407I TGTACA 1 cut(s) 601
Bsp143I GATC 3 cut(s) 267, 415, 459
Bsp1720I GCTNAGC 2 cut(s) 90, 242
BspACI CCGC 1 cut(s) 593
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 5 cut(s) 103, 165, 234, 328, 364
BspLI GGNNCC 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 2 cut(s) 275, 423
BsrFI RCCGGY 1 cut(s) 178
BsrGI TGTACA 1 cut(s) 601
BssAI RCCGGY 1 cut(s) 178
BssMI GATC 3 cut(s) 267, 415, 459
BssSI CACGAG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 230, 609
BstAUI TGTACA 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 557
BstDEI CTNAG 5 cut(s) 90, 173, 242, 315, 372
BstF5I GGATG 1 cut(s) 532
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 3 cut(s) 270, 418, 462
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 3 cut(s) 267, 415, 459
BstMWI GCNNNNNNNGC 2 cut(s) 14, 561
BstV1I GCAGC 5 cut(s) 17, 98, 106, 287, 480
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 1 cut(s) 178
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 2 cut(s) 170, 253
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 557
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 343
Csp6I GTAC 4 cut(s) 226, 445, 517, 602
CviAII CATG 4 cut(s) 214, 523, 550, 565
CviQI GTAC 4 cut(s) 226, 445, 517, 602
DdeI CTNAG 5 cut(s) 90, 173, 242, 315, 372
DpnI GATC 3 cut(s) 269, 417, 461
DpnII GATC 3 cut(s) 267, 415, 459
DrdI GACNNNNNNGTC 1 cut(s) 373
DseDI GACNNNNNNGTC 1 cut(s) 373
Ecl136II GAGCTC 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 433
FaeI CATG 4 cut(s) 217, 526, 553, 568
FaiI YATR 7 cut(s) 215, 485, 499, 524, 551, 566, 613
FatI CATG 4 cut(s) 213, 522, 549, 564
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 5 cut(s) 6, 87, 95, 276, 494
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 433
Fsp4HI GCNGC 5 cut(s) 6, 87, 95, 276, 494
GlaI GCGC 1 cut(s) 301
GluI GCNGC 5 cut(s) 6, 87, 95, 276, 494
GsaI CCCAGC 1 cut(s) 155
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 179
HhaI GCGC 1 cut(s) 302
Hin1II CATG 4 cut(s) 217, 526, 553, 568
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 4 cut(s) 110, 313, 374, 534
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 3 cut(s) 366, 391, 447
Hpy188I TCNGA 4 cut(s) 318, 373, 459, 469
Hpy188III TCNNGA 2 cut(s) 265, 341
Hpy8I GTNNAC 3 cut(s) 366, 391, 447
HpyAV CCTTC 2 cut(s) 63, 313
HpyCH4III ACNGT 2 cut(s) 230, 609
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 561
HpyF3I CTNAG 5 cut(s) 90, 173, 242, 315, 372
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 4 cut(s) 217, 526, 553, 568
HspAI GCGC 1 cut(s) 300
Kzo9I GATC 3 cut(s) 267, 415, 459
LmnI GCTCC 3 cut(s) 63, 428, 436
Lsp1109I GCAGC 5 cut(s) 17, 98, 106, 287, 480
LweI GCATC 1 cut(s) 517
MaeII ACGT 1 cut(s) 261
MalI GATC 3 cut(s) 269, 417, 461
MboI GATC 3 cut(s) 267, 415, 459
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 2 cut(s) 433, 480
MlyI GAGTC 4 cut(s) 119, 307, 383, 543
MspI CCGG 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 14, 561
NdeII GATC 3 cut(s) 267, 415, 459
NlaIII CATG 4 cut(s) 217, 526, 553, 568
NlaIV GGNNCC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 181
PkrI GCNGC 5 cut(s) 7, 88, 96, 277, 495
PleI GAGTC 4 cut(s) 118, 307, 382, 542
PpsI GAGTC 4 cut(s) 118, 307, 382, 542
Psp124BI GAGCTC 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 1 cut(s) 343
PsuI RGATCY 1 cut(s) 267
RsaI GTAC 4 cut(s) 227, 446, 518, 603
RsaNI GTAC 4 cut(s) 226, 445, 517, 602
SacI GAGCTC 1 cut(s) 433
SatI GCNGC 5 cut(s) 6, 87, 95, 276, 494
Sau3AI GATC 3 cut(s) 267, 415, 459
Sau96I GGNCC 1 cut(s) 343
SchI GAGTC 4 cut(s) 119, 307, 383, 543
SduI GDGCHC 2 cut(s) 433, 480
SfaNI GCATC 1 cut(s) 517
SinI GGWCC 1 cut(s) 343
SsiI CCGC 1 cut(s) 593
SstI GAGCTC 1 cut(s) 433
TaaI ACNGT 2 cut(s) 230, 609
TaiI ACGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 306, 474
TatI WGTACW 3 cut(s) 444, 516, 601
TscAI CASTG 2 cut(s) 177, 253
TseI GCWGC 5 cut(s) 5, 86, 94, 275, 493
TspGWI ACGGA 2 cut(s) 70, 443
TspRI CASTG 2 cut(s) 177, 253
VpaK11BI GGWCC 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.